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primary binding
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     The SOAP including of its binding and message are introduced and its primary binding mode in application:RPO/Encoding binding mode and Document/Literal binding mode.
     介绍了SOAP协议,包括消息及其绑定。 对于SOAP协议的应用,文中介绍了其主要的绑定模式:RPC/编码绑定模式及文档/文字绑定模式。
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  “primary binding”译为未确定词的双语例句
     With the selfmade and commercial probes, the number of primary binding sites of Ni(Ⅱ) on HSA was found to be 116 and 123, respectively, and the binding constant was found to be 86×105 L/mol and 75×105 L/mol, respectively. The results suggest that the inexpensive selfmade microdialysis probes could be used as the substitutes of expensive commercial probes. 
     用自制的微透析探针测定Ni(Ⅱ)与HSA之间的第一结合位点数和结合常数分别为1 16和8 6×105L/mol,采用商品的微透析探针测定的结果分别为1 23和7 5×105L/mol。
短句来源
     Results At the low pH values 4.5 and ionic strength 0.2 mol·L -1,the BSM-R8 interaction was principally electrostatic interaction,the five primary binding sites ( n 1) predominantly were supplied by sulfate groups,the secondary binding sites apparently depended on pH,in that percent ionization of sialic acid residues ( n 2) in BSM.
     结果 在pH值小于或等 于4.5和离子强度大于或等于0.2mol·L-1时,R8与BSM的相互作用主要是静电作用。 BSM分子中第一类(n1)5个结合位点主要是由硫酸基团提供,第二类(n2)结合位点明显依赖于溶液的pH值,即粘蛋白分子中唾液酸残基羧 酸根的解离程度。
短句来源
     CE-FA was also used to investigate the effect of 1 8-methyl norethindron on ketoprofen-HSA interaction and the results indicated that 18 -methyl norethindrone can not displace ketoprofen from its primary binding site while ketoprofen could be displaced by high concentration 1 8-methyl norethindrone from its second binding site.
     还应用此法研究了18-甲基炔诺酮对酮洛芬-人血清白蛋白结合作用的影响,结果发现18-甲基炔诺酮不能将酮洛芬从它的第一类结合位点上置换出来,而高浓度的18-甲基炔诺酮可将酮洛芬从它的第二类结合位点上置换出来。
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     HSA is one of the main protein involved in the binding of drugs, with two major primary binding sites located in subdomain IIA and subdomain III A.
     并且人血清白蛋白是药物的重要载体,其与药物的结合位点主要是ⅡA亚畴和ⅢA亚畴。
短句来源
     A double antibody sandwich ELISA, using 6AL42 as the primary binding antibody and conjugated rabbit anti-p27 as the reporter antibody, differentiated between endogenous RAV-O and exogenous p27 antigen in undiluted albumen samples.
     试验用McAb-6AL42作为第一抗体和兔抗P~(27)血清的过氧化物酶标记物作为第二抗体(指示抗体)建立了双抗体夹心ELISA试验。 用这种ELISA试验可区分未经稀释的卵蛋白中RAV-O和RPL-40群特异性抗原。
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  相似匹配句对
     protein binding;
     蛋白结合;
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     It was observered that the the binding of WSSV on the primary cultured cell of shrimp Penaeus.
     以原代培养的中国对虾血淋巴细胞作为基质,观察到了VP37多克隆抗体对WSSV与细胞结合的抑制作用。
短句来源
     Will is primary.
     意志是第一性的,理性则是由意志派生出来的,理性命定为意志服务。
短句来源
     primary prevention.
     初级预防。
短句来源
     Determination of Binding Calcium in Human Erythrocyte Membrane for Primary Hypertension
     原发性高血压红细胞膜结合钙的测定
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  primary binding
It is now known that, for HIV infection, as well as primary binding of virus to the CD4 receptor, entry and infection of cells requires coreceptors which are members of the chemokine group of G-protein coupled receptors.
      
The primary binding site of benzothiadiazides was designated site II, the diazepam site on the HSA molecule, as indicated by displacement experiments using different site-selective probes.
      
Its spatial structure and the conformation of its primary binding segment from Cys3I (P3) to Glu7I (P3'), which contains a reactive scissile bond Arg5I C-Ile6I N, were found to be very similar to the other squash family proteinase inhibitors.
      
Elimination of the 4-hydroxyl group in the coumarin chemical structures decreases the binding affinity of the compounds on the primary binding site of serum albumin, with 4-chromanol the smallest ligand which binds to seroalbumin with high affinity.
      
The primary binding site of C12E6 is on the lauryl subunit of this molecule.
      
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Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus ( PrC-RSV...

Hybridoma cell lines secreting monoclonal antibody ( MCA ) to avian leukosis virus ( ALV ) structural proteins p27 and p19 have been established. MCA 6AL20 ( IgG1 isotype ) reacted with RPL-40, AMV, RAV-2 and Carr-Zilber RSV ( CZ-RSV ) .representing exogenous ALV subgroups A, B and D, respectively, but not the endogenous virus RAV-0 ( subgroup E ) in an indirect enzyme-linked immunosorbent assay ( ELISA ) . MCA 6AL22 reacted as above and, in addition, reacted with the Prague strain of Rous sarcoma virus ( PrC-RSV ) , subgroup C. Both MCAs im-munoprecipitated p19 from 35S-methionine-labeled RPL-40 or RAV-1,but not RAV-O infected chi-cken embryo fibroblasts ( CEF ) . They can be used to differentiate exogenous from endogenous ( RAV-O ) infection either in an indirect antibody ELISA or by immunoprecipitation. A third MCA, 6AL42 ( IgG2a isotype), reacted with exogenous viruses at an antibody titer up to 1,000-fold higher than with endogenous subgroup E, RAV-O virus in indirect ELISAs. MCA 6AL42 immunoprecipitated p27 and the group-specific antigen precursor protein, pr76, from cells infected with RPL-40, RAV-1 or RAV-O. A double antibody sandwich ELISA, using 6AL42 as the primary binding antibody and conjugated rabbit anti-p27 as the reporter antibody, differentiated between endogenous RAV-O and exogenous p27 antigen in undiluted albumen samples.

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb-6AL42可与P~(2...

建立了分泌抗禽白血病毒(ALV)结构蛋白P~(27)和P~(19)的单克隆抗体(McAb)杂交瘤细胞。酶联免疫吸附试验(ELISA),McAb-6AL20(属于IgG_1)分别与外源性ALV:A.B.D亚群的RPL-40、AMV、RAV-2和Carr-Zilber RSV(CZ-RSV)发生特异性反应,而不与内源性ALV:RAV-O(E亚群)发生反应。McAb-6AL_(22)(属于IgG_1)除了上述特性外,还能与劳斯氏肉瘤病毒Prague株(Prc-RSV、属于C亚群)发生反应。这二个McAb均能与〔~(35)S〕甲硫氨酸标记的RPL-40和RAV-1病毒的P~(19)蛋白出现免疫沉淀。但是,它们不与RAV-O病毒的P~(19)蛋白发生免疫沉淀。所以,McAb可用于区别ALV结构蛋白P~(19)(亚群特异性位点的多肽)。应用这二个McAb进行ELISA和免疫沉淀与聚丙烯酰胺凝胶电泳试验可区分RPL-40和RAV-O感染。McAb-6AL42(属于IgG2a)可与以上几个亚群(A.B.C和D)的毒株发生反应,其ELISA的抗体滴度比与RAV-O(E亚群)毒株高1000倍以上。McAb-6AL42可与P~(27)和pr~(76)(群特异性抗原的前体蛋白)发生免疫沉淀。试验用McAb-6AL42作为第一抗体和兔抗P~(27)血清的过氧化物酶标记物作为第二抗体(指示抗体)建立了双抗体夹心ELISA试验。用这种ELISA试验可区分未经稀释的卵蛋白中RAV-O和RPL-40群特异性抗原。

In this paper, a new method-micrdialysis has been established to determine the interaction binding parameters of Cd2+ with human serum albumin (HSA). The recoveries of meta1 ions on microdialysis are Zn2+ 70.45% (RSD of 3.2%, n=6) and Cd2+ 58.36% (RSD 2.1%, n=6). The primary binding numbers and constants are: 1.81 and 3.55×105M-1 for Cd2+. Through competing binding experiments of these metal ions on HSA a way of judging the common binding sites and relative binding strength has been established....

In this paper, a new method-micrdialysis has been established to determine the interaction binding parameters of Cd2+ with human serum albumin (HSA). The recoveries of meta1 ions on microdialysis are Zn2+ 70.45% (RSD of 3.2%, n=6) and Cd2+ 58.36% (RSD 2.1%, n=6). The primary binding numbers and constants are: 1.81 and 3.55×105M-1 for Cd2+. Through competing binding experiments of these metal ions on HSA a way of judging the common binding sites and relative binding strength has been established. There is a common primary binding site of Cd2+ and Zn2+ on HSA. There is a common second binding site of mu1tip1e meta1 ions on HSA. Using this precise, quick and convenient method more than 3/4 protein can be saved.

本文建立了一种新的方法──微透析法用于研究金属离子铺与人血清蛋白(Human Serum Albumin,简称HSA)的相互作用.测定了金属离子Cd2+与HSA之间的结合位点数为1.81和结合常数3.55×105M-1微透析回收率Cd2+为58.36%(R.S.D2.1%,n=6).通过各种离子之间的竞争结合建立了通用的判定金属离子的共同结合位点及相对结合强度的方法.证明在HSA上Cd2+和Zn2+有共同的第一结合位点,它们在HSA上有一多元素的共同结合位点.应用本实验方法可以节约蛋白3/4以上,并具有精确、快速、方便等特点.

A method for the preparation of the microdialysis probe is reported. Effects of the perfusion rate, the temperature and the area of the microdialysis probe membrane on the recovery of nickel ion were studied. The selfmade microdialysis probes showed characteristics similar to those of the commercial ones. The interaction of Ni(Ⅱ) with human serum albumin (HSA) was studied by these microdialysis probes coupled with HPLC. With the selfmade and commercial probes, the number of primary binding sites of Ni(Ⅱ)...

A method for the preparation of the microdialysis probe is reported. Effects of the perfusion rate, the temperature and the area of the microdialysis probe membrane on the recovery of nickel ion were studied. The selfmade microdialysis probes showed characteristics similar to those of the commercial ones. The interaction of Ni(Ⅱ) with human serum albumin (HSA) was studied by these microdialysis probes coupled with HPLC. With the selfmade and commercial probes, the number of primary binding sites of Ni(Ⅱ) on HSA was found to be 116 and 123, respectively, and the binding constant was found to be 86×105 L/mol and 75×105 L/mol, respectively. The results suggest that the inexpensive selfmade microdialysis probes could be used as the substitutes of expensive commercial probes. 

介绍了一种自制微透析探针的制备方法,考察了灌流液流速、温度以及探针膜面积等因素对金属离子Ni(Ⅱ)回收率的影响。实验结果表明:自制微透析探针透析金属离子的各种指标都与商品的微透析探针性能相当,可以替代商品化微透析探针研究金属离子与蛋白质的相互作用。利用微透析与高效液相色谱联用技术研究了Ni(Ⅱ)与人血清白蛋白(HSA)的相互作用。用自制的微透析探针测定Ni(Ⅱ)与HSA之间的第一结合位点数和结合常数分别为1 16和8 6×105L/mol,采用商品的微透析探针测定的结果分别为1 23和7 5×105L/mol。

 
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