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   ribosome binding 的翻译结果: 查询用时:0.038秒
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ribosome binding
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  “ribosome binding”译为未确定词的双语例句
     In eukaryotes, Selenocysteine incorporation into selenoproteins requires the interaction between selenocysteine insertion sequence (SECIS) element residing in the 3' untranslated region (3'UTR) of selenoprotein mRNAs and SECIS-binding protein SBP2, containing RNA-binding domain and ribosome binding domain.
     真核生物UGA密码子解码为硒代半胱氨酸需要定位在硒蛋白mRNA 3’UTR的硒代半胱氨酸插入序列元件SECIS和SECIS结合蛋白SBP2的存在及二者之间的相互作用。
短句来源
     Methods: The TIP30 and human IFN-γ genes were amplified by PCR and inserted into eukaryotic expression vector pCI-neofor the construction of expression plasmids pCI-TIP30 and pCI-IFN, respectively. A co-expression plasmid pCI-TIP30/IFN was constructed by linking TIP30 and human IFN-γ gene using the sequence of internal ribosome binding sequence (IRES).
     方法:PCR扩增TIP30和人IFN-γ基因,分别插入真核表达载体pCI—neo,构建成重组表达质粒pCI-TIP30、pCI-IFN和TIP30、IFN-γ基因共表达质粒pCI-TIP30/IFN。
短句来源
     Sequencing of the DNA fragment revealed an open reading frame encoding 461 amino acids, homologous to known cbbM genes, with a ribosome binding site (RBS) upstream of cbbM and a terminator downstream of cbbM, without promoter.
     结果表明该DNA片段存在一个完整的阅读框架(ORF),即formⅡ RubisCO基因(cbbM),长为1386个核苷酸,共编码461个氨基酸。
短句来源
     The other ORF of 384 amino acids named ORF 1155 was related to outer membrane protein, but the ribosome binding site was not find from its upstream before initial start codon.
     ORF1155读框则与外膜蛋白相关,但起始密码子上游未发现RBS(可能与起始密码子上游序列长度太短有关),因此无法判断是否是一个真正的ORF;
短句来源
     Sequence structure of Hepatitis B virus (HBV adrNC-l) DNA. was analyzed by computer to compare its sequence homology with other related DNA and search for restriction enzyme sites, reading frame, feature sequence of eukaryotic organism, hairpin loop and eukaryotic ribosome binding sites,etc. It proved that using computer to analyze nucleic acid sequence is one of the important technics in molecular biology research, since these data were obtained with VAX-11/730 computer in ou institute.
     以乙肝病毒DNA序列分析为例,较详细地介绍了电子计算机在分析DNA序列的同源性,寻找内切酶识别位点,开放读码框架,真核生物的特征顺序,发卡环,真核核糖体RNA结合位点等方面所进行的工作,这些工作是在我所VAX-11/730计算机上完成的。
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  相似匹配句对
     protein binding;
     蛋白结合;
短句来源
     Effect of Ribosome Binding Site Sequence on the Expression Level of HBcAg in E. colt
     核糖体结合位点序列对大肠杆菌中HBcAg重组质粒表达的影响
短句来源
     EF1α·GTP catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome.
     GTP催化氨酰tRNA结合到核糖体的A位点。
短句来源
     COM AUTOMATION BINDING
     COM自动化技术的绑定
短句来源
     Ribosome is widely distributed.
     核糖体比比皆是。
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  ribosome binding
In this work, we examined the ability of artificial ribosome binding sites (RBSs) containing such an extended (10-nt) SD-sequence (super-SD) to drive translation in vivo, as well as its ability to form the translation initiation complex in vitro.
      
coli promoter's -35 and -10 region respectively, as well as ribosome binding site GGAGG at position-12--8 upstream the ATG were determined by SangerDideoxyribonucleotide-mediated chain-termination method.
      
In contrast, the loop modification, 1-methylguanosine, enhanced ribosome binding, but dramatically decreased thermal stability.
      
Thus, effective ribosome binding of tRNAPhe is a combination of anticodon stem stability and the correct architecture and dynamics of the anticodon loop.
      
The noncoding flanking sequences of HIV structural genes were removed and a putative ribosome binding site was placed in front of the open reading frame of each gene by using crossover linker mutagenesis.
      
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Sequence structure of Hepatitis B virus (HBV adrNC-l) DNA. was analyzed by computer to compare its sequence homology with other related DNA and search for restriction enzyme sites, reading frame, feature sequence of eukaryotic organism, hairpin loop and eukaryotic ribosome binding sites,etc. It proved that using computer to analyze nucleic acid sequence is one of the important technics in molecular biology research, since these data were obtained with VAX-11/730 computer in ou institute.

以乙肝病毒DNA序列分析为例,较详细地介绍了电子计算机在分析DNA序列的同源性,寻找内切酶识别位点,开放读码框架,真核生物的特征顺序,发卡环,真核核糖体RNA结合位点等方面所进行的工作,这些工作是在我所VAX-11/730计算机上完成的。

The conserved regions in promoters, ribosome-binding sites, teminator sequence and splice sites for each category of sequences are obtained by statistical analysis. On account of the possible important rule of these conserved sites on the regulation of gene expression we have calculated the lecal deviations of double helix structures of DNAs and compared them with conserved sites.

统计分析了12类核酸序列在起始密码子邻近,终止密码子邻近以及内含子两端的保守区,得到了保守区随序列类别变化的经验规律,鉴于这些保守位点对基因表达的调控可能具有重要作用,本文利用Tung-Harvey模型,计算了核酸双螺旋结构的局部偏差,企图发现局部偏差相对较大的位点与保守位点之间的关系。

The recombinant (pKL series) Plasmids showing different levels of HBcAg antigenicity in ELISA were constructed by inserting the HBcAg gene which was randomly deleted at the non-coding region by Ba/-31 exonuclease into plasmid vector pKK223-3 containing tac promoter and SD sequence. SDS-PAGE and Western blot experiments indicated that molecular weight of the HBcAg protein generated by these positive clones was 21000D. Three plasmids with high, moderate and low HBcAg expression level were sequenced and the distance...

The recombinant (pKL series) Plasmids showing different levels of HBcAg antigenicity in ELISA were constructed by inserting the HBcAg gene which was randomly deleted at the non-coding region by Ba/-31 exonuclease into plasmid vector pKK223-3 containing tac promoter and SD sequence. SDS-PAGE and Western blot experiments indicated that molecular weight of the HBcAg protein generated by these positive clones was 21000D. Three plasmids with high, moderate and low HBcAg expression level were sequenced and the distance between SD sequence and HBcAg gene ATG codon was 12, 13 and 19bp respectively. Computer analysis of secondary structure of the ribosome binding sites on RNAtranscripts also revealed energy and structure differences between the low and high level expression plasmids, suggesting the importance of this distance and the mRNA structure to gene expression.

用Bal-31外切酶调整乙肝病毒核心抗原(HBcAg)基因非编码区长度并克隆到质粒pKK 223-3中,获得了不同水平地表达HBcAg的重组质粒pKL系统。该系统所表达的HBcAg蛋白分子量为21000D。DNA序列分析发现高、中、低表达水平的3个重组质粒的SD序列到HBcAg基因的ATG之间的距离分别为12bp、13bp和19bp,高、低表达质粒的mRNA核糖体结合位点序列的二级结构分析显示,二者的自由能相差约3倍,且低表达质粒的mRNA的SD序列中的3个碱基及AUG中的3个碱基都参与配对,而高表达质粒只有SD序列中的2个碱基参与配对,表明SD序列到ATG的距离及mRNA的二级结构均在HBcAg的表达调控中起重要作用。

 
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