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   ribosome binding 在 生物学 分类中 的翻译结果: 查询用时:0.061秒
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ribosome binding
相关语句
  核糖体结合
    Effect of Ribosome Binding Site Sequence on the Expression Level of HBcAg in E. colt
    核糖体结合位点序列对大肠杆菌中HBcAg重组质粒表达的影响
短句来源
    After cut down by restriction enzyme,the dhaT gene with own ribosome binding site was inserted into recombining plasmid pMD19-T Simple/gldABC in tandem. Thereafter,the gene of gldABC and dhaT was subcloned into expression vector pET28a(+).
    将限制性内切酶处理后的含有自身核糖体结合位点的dhaT基因插入到质粒pMD19-T Simple/gldABC中gldABC基因的下游,形成重组克隆质粒pMD19-T Simple/gldABC-dhaT,并进一步将gldABC与dhaT串联基因亚克隆到表达载体pET28a(+)上。
短句来源
    Used promoter P59 as promoter and pGDVM as framework, get Ribosome Binding Site and terminator were got by PCR amplication from pAX01 were cloned to pGDVM , then got the shuttle vector GJ01. Use bga as report gene to detect its express ability, the highest activity of Bga in E.
    以P59为启动子,以构建的pGDVM为骨架,从pAX01上PCR扩增核糖体结合位点RBS1和终止子序列克隆到pGDVM上,即构建成大肠杆菌和芽孢杆菌之间的穿梭载体GJ01。
短句来源
    This region contained the sequences TTGAG and ATCATA which were consistent with those of E. coli promoters 35 and 10 region,respectively,as well as the ribosome binding site GGAGG at upstream position 12 ̄ 8 of ATG.
    在这一区域可见与大肠杆菌(E.coli)-35和-10区同源的碱基顺序TTGAG和ATCATA,以及ATG上游-12~-8处的核糖体结合位点GGAGG。
短句来源
    Used pGDVM as framework,P59 promoter,ribosome binding site(SD) and terminator were cloned to pGDVM,and then got the shuttle expression vector GJ01.Used bga as report gene to detect GJ01,the highest activity of Bga in E.
    在pGDVM上进行载体表达元件的构建,先后将P59启动子、核糖体结合位点SD和终止子克隆到pGDVM上得到穿梭表达载体GJ01。
短句来源
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  “ribosome binding”译为未确定词的双语例句
    In eukaryotes, Selenocysteine incorporation into selenoproteins requires the interaction between selenocysteine insertion sequence (SECIS) element residing in the 3' untranslated region (3'UTR) of selenoprotein mRNAs and SECIS-binding protein SBP2, containing RNA-binding domain and ribosome binding domain.
    真核生物UGA密码子解码为硒代半胱氨酸需要定位在硒蛋白mRNA 3’UTR的硒代半胱氨酸插入序列元件SECIS和SECIS结合蛋白SBP2的存在及二者之间的相互作用。
短句来源
    Sequence structure of Hepatitis B virus (HBV adrNC-l) DNA. was analyzed by computer to compare its sequence homology with other related DNA and search for restriction enzyme sites, reading frame, feature sequence of eukaryotic organism, hairpin loop and eukaryotic ribosome binding sites,etc. It proved that using computer to analyze nucleic acid sequence is one of the important technics in molecular biology research, since these data were obtained with VAX-11/730 computer in ou institute.
    以乙肝病毒DNA序列分析为例,较详细地介绍了电子计算机在分析DNA序列的同源性,寻找内切酶识别位点,开放读码框架,真核生物的特征顺序,发卡环,真核核糖体RNA结合位点等方面所进行的工作,这些工作是在我所VAX-11/730计算机上完成的。
短句来源
    The 5' untranslated leader region is 194bp, the putative ribosome binding site in this region is GGAGG, located immediately upstream of the start codon.
    5’前导区长194bp,其中SD序列为GGAGG,紧邻起始密码子上游.
短句来源
    One of the fragments (called SP2) contains both sequences of promoter and ribosome binding site (RBS),while the another fragment (called SP1) contains not only promoter and RBS but also the signal peptide coding sequence.
    第二种不仅含有第一种DNA片段的全部序列,而且还有贮藏蛋白的信号肽编码序列(SP1)。
短句来源
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  ribosome binding
In this work, we examined the ability of artificial ribosome binding sites (RBSs) containing such an extended (10-nt) SD-sequence (super-SD) to drive translation in vivo, as well as its ability to form the translation initiation complex in vitro.
      
coli promoter's -35 and -10 region respectively, as well as ribosome binding site GGAGG at position-12--8 upstream the ATG were determined by SangerDideoxyribonucleotide-mediated chain-termination method.
      
In contrast, the loop modification, 1-methylguanosine, enhanced ribosome binding, but dramatically decreased thermal stability.
      
Thus, effective ribosome binding of tRNAPhe is a combination of anticodon stem stability and the correct architecture and dynamics of the anticodon loop.
      
The noncoding flanking sequences of HIV structural genes were removed and a putative ribosome binding site was placed in front of the open reading frame of each gene by using crossover linker mutagenesis.
      
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Sequence structure of Hepatitis B virus (HBV adrNC-l) DNA. was analyzed by computer to compare its sequence homology with other related DNA and search for restriction enzyme sites, reading frame, feature sequence of eukaryotic organism, hairpin loop and eukaryotic ribosome binding sites,etc. It proved that using computer to analyze nucleic acid sequence is one of the important technics in molecular biology research, since these data were obtained with VAX-11/730 computer in ou institute.

以乙肝病毒DNA序列分析为例,较详细地介绍了电子计算机在分析DNA序列的同源性,寻找内切酶识别位点,开放读码框架,真核生物的特征顺序,发卡环,真核核糖体RNA结合位点等方面所进行的工作,这些工作是在我所VAX-11/730计算机上完成的。

The recombinant (pKL series) Plasmids showing different levels of HBcAg antigenicity in ELISA were constructed by inserting the HBcAg gene which was randomly deleted at the non-coding region by Ba/-31 exonuclease into plasmid vector pKK223-3 containing tac promoter and SD sequence. SDS-PAGE and Western blot experiments indicated that molecular weight of the HBcAg protein generated by these positive clones was 21000D. Three plasmids with high, moderate and low HBcAg expression level were sequenced and the distance...

The recombinant (pKL series) Plasmids showing different levels of HBcAg antigenicity in ELISA were constructed by inserting the HBcAg gene which was randomly deleted at the non-coding region by Ba/-31 exonuclease into plasmid vector pKK223-3 containing tac promoter and SD sequence. SDS-PAGE and Western blot experiments indicated that molecular weight of the HBcAg protein generated by these positive clones was 21000D. Three plasmids with high, moderate and low HBcAg expression level were sequenced and the distance between SD sequence and HBcAg gene ATG codon was 12, 13 and 19bp respectively. Computer analysis of secondary structure of the ribosome binding sites on RNAtranscripts also revealed energy and structure differences between the low and high level expression plasmids, suggesting the importance of this distance and the mRNA structure to gene expression.

用Bal-31外切酶调整乙肝病毒核心抗原(HBcAg)基因非编码区长度并克隆到质粒pKK 223-3中,获得了不同水平地表达HBcAg的重组质粒pKL系统。该系统所表达的HBcAg蛋白分子量为21000D。DNA序列分析发现高、中、低表达水平的3个重组质粒的SD序列到HBcAg基因的ATG之间的距离分别为12bp、13bp和19bp,高、低表达质粒的mRNA核糖体结合位点序列的二级结构分析显示,二者的自由能相差约3倍,且低表达质粒的mRNA的SD序列中的3个碱基及AUG中的3个碱基都参与配对,而高表达质粒只有SD序列中的2个碱基参与配对,表明SD序列到ATG的距离及mRNA的二级结构均在HBcAg的表达调控中起重要作用。

By using site-directed mutagenesis, rabbit liver cytochrome b5 mRNA was modified for the in vitro expression of both liver and erythrocyte cytochrome b5s.A prokaryotic ribosome binding site 10 base pair from the start codcn ATG and a premature stop codon at amino acid position 99 were introduced.Using guinea pig antibody against rabbit liver cytochrome b5 we monitored the synthesis or both form of b5s on western blotting after inducing the expression vectors with IPTG The inducib-le level were 1.5********ug(liver)and...

By using site-directed mutagenesis, rabbit liver cytochrome b5 mRNA was modified for the in vitro expression of both liver and erythrocyte cytochrome b5s.A prokaryotic ribosome binding site 10 base pair from the start codcn ATG and a premature stop codon at amino acid position 99 were introduced.Using guinea pig antibody against rabbit liver cytochrome b5 we monitored the synthesis or both form of b5s on western blotting after inducing the expression vectors with IPTG The inducib-le level were 1.5********ug(liver)and 4.5*******ug(erythrocyte)b5/mL of culture.

利用体外特定点突变技术,将原核生物核糖体结合位点及十碱基间隔子(spacer)引科兔肝经胞色素b_5 cDNA的5′端,并构建了一个兔红血球b_5cDNA克隆,重组入表达载体pKK223-3质粒中,在大肠杆菌中成功地表达了兔肝和红血球两种形式的b_5蛋白。产量分别为每毫升培养液1.5μg和4.5μg。

 
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