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interferon expression
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  “interferon expression”译为未确定词的双语例句
     Results: The extract of compound Radix Scutellariae could inhibit influenza virus's mRNA replication(P<0.01) and induce interferon expression in mice(P>0.05).
     结果:复方黄芩提取物对小鼠体内流感病毒mRNA表达有较好的抑制作用(P<0.01),对干扰素的表达有诱生作用(P>0.05)。
短句来源
     The Difference of Interleukin-4 and Gamma Interferon Expression in Helicobacter Pylori-Infected Subjects and Its Effect on Eradication Therapy
     H.pylori感染者IL-4和IFN-γ表达及其对根除治疗影响的研究
短句来源
     Results:Dot hybridization of total RNA from the neomycin resistant colonies and interferon expression assay indicated that human α-fetoprotein enhancer induced efficient and specific transcription and expression of IFN-β gene drived by the promoter of different origin in the human hepatoma cells in which α-fetoprotein was highly produced.
     结果:NeoR克隆RNA斑点杂交及IFN活性分析证明,人AFPe可促进异源启动子启动HuIFN-β基因在合成AFP的人肝癌细胞中高效特异转录和表达。
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  相似匹配句对
     The expression
     COX-2、VEGF-C mRNA的表达与肿瘤TNM
短句来源
     The expression of E.
     研究了E.
短句来源
     Interferon
     干扰素
短句来源
     Expression and purification of recombinant interferon-gamma
     重组γ干扰素的表达与纯化
短句来源
     Cloning and expression of yak interferon-β gene
     牦牛β-干扰素基因的克隆与表达
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  interferon expression
Hepatic α-interferon expression in cytomegalovirus-infected liver allograft recipients with and without vanishing bile duct synd
      
Interferon expression in the testes of transgenic mice leads to sterility.
      
Interferon expression in the pancreases of patients with type I diabetes.
      


Objective:To study the hepatoma cell-specific expression of human interferon gene mediated by retroviral vectors.Methods:Human interferon-β complementary DNA (IFN-β cDNA) was inserted into polylinker site of PMNSM retroviral vector to construct recombinant retroviral vector pMNSIFNB,in which the transcription of IFN-β gene was drived by SV40 early region promoter,and MNAIFNB,in which the transcription of IFN-β gene was drived by SV 40 early region promoter regulated by α-fetoprotein enhancer.The retroviral constructs...

Objective:To study the hepatoma cell-specific expression of human interferon gene mediated by retroviral vectors.Methods:Human interferon-β complementary DNA (IFN-β cDNA) was inserted into polylinker site of PMNSM retroviral vector to construct recombinant retroviral vector pMNSIFNB,in which the transcription of IFN-β gene was drived by SV40 early region promoter,and MNAIFNB,in which the transcription of IFN-β gene was drived by SV 40 early region promoter regulated by α-fetoprotein enhancer.The retroviral constructs were respectively introduced into PA317 amphotropic packaging cells by means of lipofect AMINETM mediated gene transfer procedure.The plasmids transfection efficiency was among(4 ̄25)×103 colonies/μg DNA/106 PA317 cells.The retrovirus infection efficiency was among(4.5 ̄500)×104 Coloney Forming Units(CFU)/ml.The recombinant retroviruses were used to infect human hepatoma cells,renal cell carcinoma cells and melanoma cell lines in the presence of 4 μg/ml polybrene.Results:Dot hybridization of total RNA from the neomycin resistant colonies and interferon expression assay indicated that human α-fetoprotein enhancer induced efficient and specific transcription and expression of IFN-β gene drived by the promoter of different origin in the human hepatoma cells in which α-fetoprotein was highly produced. Conclusion:Cis-active element of α-fetoprotein gene can drive IFN-β gene specifically expressed in the human hepatoma cells,which present some valuable materials for the hepatoma-specific immune gene therapy.

目的:研究逆转录病毒介导干扰素基因转移及在肝癌细胞中特异表达作用。方法:将人β干扰素(HuIFN-β)cDNA克隆到逆转录病毒载体PL位点,分别构建了转录受SV40启动子驱动的载体MNSIFNB和转录受人甲胎蛋白增强子(AFPe)调控的载体MNAIFNB。用脂质体转染法将载体分别转导PA317包装细胞,质粒转染率为每105PA317细胞(4~25)×103CFU/μg,病毒感染率(4.5~500)×104CFU/ml。重组病毒在4μg/mlpolybrene存在条件下感染人肝癌、肾癌及黑色素瘤细胞。结果:NeoR克隆RNA斑点杂交及IFN活性分析证明,人AFPe可促进异源启动子启动HuIFN-β基因在合成AFP的人肝癌细胞中高效特异转录和表达。结论:AFP"持家基因"顺式调控序列在合成AFP的肝癌细胞中特异驱动IFN-β基因表达,该研究为肝癌特异性免疫基因治疗提供了资料。

AIM To establish the hepatoma cell specific expression of human interferon gene mediated by retroviral vectors. METHODS Human interferon α and interforon β complementary DNA (IFNs cDNA) were cloned into polylinker site of pMNSM retroviral vector to construct recombinant retroviral vector pMNSIFNA and pMNSIFNB, where the transcription of IFN gene was driven by SV40 early region promoter, and pMNAIFNA, pAMNSIFNA and pMNAIFNB, where the transcription of IFN gene was driven by SV40 early region promoter regulated...

AIM To establish the hepatoma cell specific expression of human interferon gene mediated by retroviral vectors. METHODS Human interferon α and interforon β complementary DNA (IFNs cDNA) were cloned into polylinker site of pMNSM retroviral vector to construct recombinant retroviral vector pMNSIFNA and pMNSIFNB, where the transcription of IFN gene was driven by SV40 early region promoter, and pMNAIFNA, pAMNSIFNA and pMNAIFNB, where the transcription of IFN gene was driven by SV40 early region promoter regulated by α fetoprotein enhancer. The retroviral constructs were respectively introduced into retroviral amphotropic packaging cells by means of lipofectamine mediated gene transfer procedure. The plasmids transfection rate was (4~40)×10 3 colonies/μg DNA/10 6 PA317 cells. The retrovirus infection rate was (5~500)×10 4 colony forming units (CFU)/ml. The recombinant retroviruses were used to infect human hepatoma cells, renal carcinoma cells and melanoma cell lines in the presence of 4mg/L polybrene. RESULTS Northern and Dot hybridization of total RNA from the neomycin resistant colonies and interferon expression assay indicated that human α fetoprotein enhancer induced efficient and apecific transcription and expression of IFNs gene driven by the promoter of different origin in human hepatoma cells by which α fetoprotein was highly produced. CONCLUSION Cis active element of α fetoprotein gene can drive specific expression of IFNs gene in human hepatoma cells, which provides some valuable data for the hepatoma specific immune gene therapy.

ConstructionofretroviralvectorstoinduceastrongexpresionofhumanclasⅠinterferongeneinhumanhepatocelularcarcinomacelsinvitroCAOG...

Objective: To study the extract of compound Radix Scutellariae on inhibiting the mRNA replication and inducing the INF expression of influenza virus in mice's vivo.Methods: Influenza virus infected mice pneumono-adaption stock A/FM/1/47(H1N1) evoked mice pneumonia taken as animal model.RT-PCR was adopted to measure the content of influenza virus′s mRNA and INF in mice to study the effect of the extract on mRNA replication and IFN expression.Results: The extract of compound Radix Scutellariae could inhibit influenza...

Objective: To study the extract of compound Radix Scutellariae on inhibiting the mRNA replication and inducing the INF expression of influenza virus in mice's vivo.Methods: Influenza virus infected mice pneumono-adaption stock A/FM/1/47(H1N1) evoked mice pneumonia taken as animal model.RT-PCR was adopted to measure the content of influenza virus′s mRNA and INF in mice to study the effect of the extract on mRNA replication and IFN expression.Results: The extract of compound Radix Scutellariae could inhibit influenza virus's mRNA replication(P<0.01) and induce interferon expression in mice(P>0.05).Conclusion: The extract of compound Radix Scutellariae can induce interferon and inhibit influenza virus replication in mice.

目的:研究复方黄芩提取物对小鼠体内流感病毒(influenza virus,Ⅳ)mRNA复制的抑制作用和干扰素(interferon,IFN)表达的诱生作用。方法:以流感病毒鼠肺适应株A/FM/1/47(H1N1)感染的小鼠肺炎为模型,采用RT-PCR技术测定小鼠体内流感病毒mRNA和干扰素含量。观察复方黄芩提取物对小鼠体内流感病毒mRNA复制和IFN表达的影响。结果:复方黄芩提取物对小鼠体内流感病毒mRNA表达有较好的抑制作用(P<0.01),对干扰素的表达有诱生作用(P>0.05)。结论:复方黄芩提取物具有诱生干扰素、抑制流感病毒在小鼠体内的复制作用。

 
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