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detecting concentration
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  检出浓度
    〔Results〕 The recovery of daidzin genistin,daidzein and genistein was 90.4%~102%,88.9%~102%,93.0%~102% and 89.3%~104%,respectively,and the variation coefficient was below 6% for all. The minimal detecting concentration of the 4 soy isoflavones was 0.10 mg/ml.
    〔结果〕黄豆甙 ,染料木甙 ,黄豆甙元和金雀异黄素的加标回收率分别为 90 .4%~ 10 2 % ,88.9%~ 10 2 % ,93 .0 %~ 10 2 % ,89.3 %~ 10 4% ,加标回收相对标准偏差均小于 6% ,最底检出浓度均为 0 .10 μg/ml。
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  “detecting concentration”译为未确定词的双语例句
    The competitive ELISA for detecting the citrinin content in the Hongquproduct was developed, with the detecting concentration range 5.0~200ng/ml of citrinin. The recovery of this method is 97%~104%.
    建立竞争ELISA检测红曲样品桔霉素含量的方法,研究了该法的特异性和桔霉素的加标回收,结果表明,此法具有较高的特异性和加标回收能力,桔霉素的测定浓度范围为5.0~200ng/ml,回收率97%~104%。
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    Objective To explore the method of detecting concentration of bright blue in bitter gourd beer by HPLC.
    目的 探讨利用高效液相色谱法快速测定啤酒中亮蓝的方法。
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  detecting concentration
Assessing the compatibility of many trace elements by conventional methods that rely on detecting concentration changes is extremely difficult.
      
Therefore, 23Na-MRI has the potential to non-invasively differentiate viable from non-viable tissue by detecting concentration changes of intra- and extracellular sodium.
      


A human Ig*#G was incorporated into the humanized milk powder. A rough human Ig*#G from the soundy humanserum was extracted by saturated ammonia sulfate,then the rough human Ig*#G was pured through sephadex G-100 column. The antibody of antihuman Ig*#G was made with pured human Ig*#G injected in rabbit. A enzymelinked immunosorbet assay of human Ig*#G content in humanized milk powder was developed, with a optimal concentration of coated micropore plate of 5.25*!μg/ml,a optimal titration of antihuman Ig*#G serum...

A human Ig*#G was incorporated into the humanized milk powder. A rough human Ig*#G from the soundy humanserum was extracted by saturated ammonia sulfate,then the rough human Ig*#G was pured through sephadex G-100 column. The antibody of antihuman Ig*#G was made with pured human Ig*#G injected in rabbit. A enzymelinked immunosorbet assay of human Ig*#G content in humanized milk powder was developed, with a optimal concentration of coated micropore plate of 5.25*!μg/ml,a optimal titration of antihuman Ig*#G serum of 1∶1*!600,a affinity of antihuman Ig*#G of 2.40×107,the recovery of 88%~94%,a detecting concentration of human Ig*#G in 5.0~160*!μg/ml and a minimal limite of detecteing concentration of 3.25*!μg/ml.

母乳化乳粉中添加保健功能因子人IgG。用饱和硫酸铵法从健康人血清中制备粗IgG ,经SephadexG -1 0 0柱制备纯IgG ;用纯人IgG作为抗原 ,免疫兔子制备兔抗人IgG抗体。建立乳制品中人IgG含量酶联免疫吸附测定 ,最佳包板物浓度为 5.2 5μg/ml,最佳血清效价为 1∶1 60 0 ,抗血清亲和力为 2 .4 0× 1 0 7,回收率为 88%~ 94 % ,测定IgG浓度范围为 5.0~1 60ng/ml,最小检测限为 3 .2 5ng/ml。

The immune antigen of OV-citrinin and the coated antigen of BSA-citrinin were synthesized respectively andidentified with ultraviolet absorption spectrometry. The competitive ELISA for detecting the citrinin content in the Hongquproduct was developed, with the detecting concentration range 5.0~200ng/ml of citrinin. The recovery of this method is 97%~104%.

制备了牛血清白蛋白——桔霉素包被抗原和卵蛋白——桔霉素免疫抗原,用卵蛋白——桔霉素连接物免疫小白鼠,间接ELISA测小鼠抗血清效价,待血清效价达到要求后,腹腔注射骨髓瘤细胞,10~15d后抽取腹水离心得抗体。建立竞争ELISA检测红曲样品桔霉素含量的方法,研究了该法的特异性和桔霉素的加标回收,结果表明,此法具有较高的特异性和加标回收能力,桔霉素的测定浓度范围为5.0~200ng/ml,回收率97%~104%。

〔Objective〕To develop a method based on HPLC-PDA for determination of 4 soy isoflavones in health foods.〔Methods〕 After sonicated for 30 min and filtered thromgh the 0.45 um filter,the samples were separated on a C18 reversed-phase column using methanol:0.050 mol/L ammonium acetate pH4.6 as the mobile phase at a velocity of 1.2 ml/min and detected by photodiode-array detector at 210nm-400nm.Good separation of the daidzin genistin,daidzein and genistein was achieved in about 20 min.〔Results〕 The recovery of daidzin...

〔Objective〕To develop a method based on HPLC-PDA for determination of 4 soy isoflavones in health foods.〔Methods〕 After sonicated for 30 min and filtered thromgh the 0.45 um filter,the samples were separated on a C18 reversed-phase column using methanol:0.050 mol/L ammonium acetate pH4.6 as the mobile phase at a velocity of 1.2 ml/min and detected by photodiode-array detector at 210nm-400nm.Good separation of the daidzin genistin,daidzein and genistein was achieved in about 20 min.〔Results〕 The recovery of daidzin genistin,daidzein and genistein was 90.4%~102%,88.9%~102%,93.0%~102% and 89.3%~104%,respectively,and the variation coefficient was below 6% for all.The minimal detecting concentration of the 4 soy isoflavones was 0.10 mg/ml.〔Conclusions〕 This method is suitable for the determination of soy isoflavones in health foods.

〔目的〕检测保健食品中黄豆甙、黄豆甙元、染料木甙和金雀异黄素 ,建立保健食品中四种大豆异黄酮的二极管阵列检测器高效液相色谱法。〔方法〕样品用甲醇 +水 (80 + 2 0 ,v/v)超声提取 3 0min ,定容后过 0 .45 μm滤膜 ,上 2 5 0×4.6mm ,粒径 10 μm的C18柱 ,以甲醇 + 0 .0 5 0mol/L ,pH4.6醋酸铵 (4 6+ 5 4,v/v)作流动相 ,二极管阵列检测器测定。测定时间只需 2 0min左右 ,黄豆甙 ,染料木甙 ,黄豆甙元和金雀异黄素可得到良好分离。〔结果〕黄豆甙 ,染料木甙 ,黄豆甙元和金雀异黄素的加标回收率分别为 90 .4%~ 10 2 % ,88.9%~ 10 2 % ,93 .0 %~ 10 2 % ,89.3 %~ 10 4% ,加标回收相对标准偏差均小于 6% ,最底检出浓度均为 0 .10 μg/ml。〔结论〕本方法适用于保健食品中大豆异黄酮的测定

 
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