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detecting concentration
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  检测浓度
    Detecting different concentration of L.interrogans blended into normal animal tissue with genechip and PCR,their minimum detecting concentration were 10 and 100 leptospiras, respectively. The sensibility of genechip was higher than that of routine PCR.
    用芯片和PCR对动物组织中不同浓度 钩端螺旋体的检测,最低检测浓度分别为10 条和100条钩端螺旋体,芯片检测的敏感性 高于常规PCR法。
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  detecting concentration
Assessing the compatibility of many trace elements by conventional methods that rely on detecting concentration changes is extremely difficult.
      
Therefore, 23Na-MRI has the potential to non-invasively differentiate viable from non-viable tissue by detecting concentration changes of intra- and extracellular sodium.
      


Different oligonucleotide probes or controls were spotted on glass slides in quarteticate with a low density array format.The fluorescent-labeled PCR products were prepared by incorporation of Cy3-labeled primer during PCR amplification and were hybridized to the genechips for detection of Leptospira interrogans strains and others.Their sensibility,specificity and feasibility were compared with conventional PCR.A specific amplification product with 482 bp length could be found from L.interrogans by conventional...

Different oligonucleotide probes or controls were spotted on glass slides in quarteticate with a low density array format.The fluorescent-labeled PCR products were prepared by incorporation of Cy3-labeled primer during PCR amplification and were hybridized to the genechips for detection of Leptospira interrogans strains and others.Their sensibility,specificity and feasibility were compared with conventional PCR.A specific amplification product with 482 bp length could be found from L.interrogans by conventional PCR,and conversely for the other microorganisms and normal animal tissue.The results showed no difference between genechip hybridization and conventional PCR.Detecting different concentration of L.interrogans blended into normal animal tissue with genechip and PCR,their minimum detecting concentration were 10 and 100 leptospiras, respectively. The sensibility of genechip was higher than that of routine PCR.Detecting the kidney,liver,heart and blood of 5 infected guinea pigs with genechip and PCR,their results were positive;Detecting 28 rat kidney specimens from epidemic area by the two methods,8 and 4 were positive, respectively. Detecting 10 kidney specimens of suspicious infected pigs,7 and 5 were positive, respectively.Detecting 5 rat kidney specimens from non-epidemic area, 5 normal kidney specimens and other microbe,the results were negative.These results revealed that genechip technique could rapidly, sensitively,specifically detect L.interrogans in animal source of infection.

为了将合成的核酸探针点样到玻片 上制备成基因芯片,先用常规PCR扩增动物 组织中钩端螺旋体特异片段,再用Cy3标记 引物,通过不对称PCR获取荧光素标记的靶 序列,然后与制备的芯片杂交,扫描仪记录结 果。同时,以PCR技术为对照,进一步观察 基因芯片技术检测动物传染源中钩端螺旋体 的特异性、敏感性及可行性。常规PCR扩增 问号钩端螺旋体阳性标本,出现单一482bp 的扩增产物,而双曲钩端螺旋体及其他螺旋 体、病原、正常动物组织均无任何DNA扩增 条带,芯片杂交结果与常规PCR方法结果一 致。用芯片和PCR对动物组织中不同浓度 钩端螺旋体的检测,最低检测浓度分别为10 条和100条钩端螺旋体,芯片检测的敏感性 高于常规PCR法。用基因芯片与PCR分别 对标本进行检测,5只人工感染的豚鼠肾、 肝、心、血等组织的检测结果均为阳性;28份 疫区野鼠肾标本阳性结果分别为8份和4 份,10份可疑病猪肾标本阳性结果分别7份 和5份;5份非疫区野鼠肾、5份正常猪肾标 本以及其他微生物的检测结果均为阴性。结 果表明,基因芯片技术可快速、灵敏、特异地 检测动物传染源中问号钩端螺旋体。

Objective To detect concentration of serum leptin and tumor necrosis factor-α(TNF-α) in patients with active lung tuberculosis and to analysis their correlation.Methods Serum leptin and TNF-α concentrations were measured by radioimmunossay in 48 patients with lung tuberculosis and in 30 controls.Cell mediated immunity was also measured,the correlation between leptin and other parameters was analyzed.Results Serum leptin concentration in 48 patients with lung tuberculosis was higher significantly...

Objective To detect concentration of serum leptin and tumor necrosis factor-α(TNF-α) in patients with active lung tuberculosis and to analysis their correlation.Methods Serum leptin and TNF-α concentrations were measured by radioimmunossay in 48 patients with lung tuberculosis and in 30 controls.Cell mediated immunity was also measured,the correlation between leptin and other parameters was analyzed.Results Serum leptin concentration in 48 patients with lung tuberculosis was higher significantly than that in control,serum TNF-α concentration was higher than in control, too.There was no correlation between TNF-α and leptin.Compared with control,T-lymphocyte subsets and total lymphocyte count decreased obviously,and serum leptin level was negatively correlated with the parameters for cell-mediatecl immunity.Conclusion The higher concentration of serum leptin in patients with lung tuberculosis perhaps involved in the decrease of T cell-mediated immunity.

目的检测肺结核患者血清瘦素(leptin)和肿瘤坏死因子-α(TNF-α)浓度并分析其相关性。方法采用放射免疫法测定48例活动性肺结核患者和30例正常人的血清瘦素和TNF-α。同时测定两组实验者的细胞免疫功能,分析瘦素与这些参数的相关性。结果肺结核患者的血清瘦素[(3.0±1.74)μg/L]水平显著高于正常对照组[(1.32±0.18)μg/L(P<0.01)]。而TNF-α血清浓度[(1.8±0.84)μg/L]也高于对照组[(0.79±0.26)μg/L](P<0.01),两组间无显著相关性。肺结核患者的T细胞亚群CD3+T、CD4+T、CD8+T和总淋巴细胞计数显著下降,且与血清瘦素呈负相关关系。结论肺结核患者的血清高瘦素水平可能参与了细胞免疫功能的降低。

 
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