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expression could
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     Result RT-PCR detection showed that THNAK gene expressed in PBMCs after stimulated with interferon-γfor 3 days, whereas THANK'expression could not be detected after stimulated with LPS,TNF-α,IL-2,IFN-γ,PHA or PMA respectively.
     结果RT-PCR分析表明,当用IFN-γ刺激PMBC 72h后,可诱导THANK基因明显表达; 而IL-2、LPS、TNF-α、PHA和PMA则不能诱导其表达。
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     Green fluorescent protein (GFP) expression could be observed on the third day after packing of the linearized pAdHGF and pAduPA in 293 cells and 4×10 10 efu/ml titer of AdHGF and 6×10 10 efu/ml titer of AduPA were obtained by CsCl gradient purification respectively.
     经 2 93细胞包装 ,3d后均观察到绿色荧光蛋白明显表达 ,Cs Cl梯度离心纯化最终分别获得 4× 10 1 0 efu/ m l滴度的重组病毒 Ad HGF和 6× 10 1 0 efu/ ml Adu PA;
短句来源
     GFP expression could be observed on the third day after packing of the linearized pAdHGF in 293 cells and 4×10 10 efu/ml titer of AdHGF was obtained by CsCl gradient purification.
     经 2 93细胞包装 ,3d后观察到绿色荧光蛋白 (GFP)明显表达 ,氯化铯梯度离心纯化最终获得约 4× 10 10 efu/ml滴度的重组病毒 ;
短句来源
     GFP expression could be observed on the third day after packing of the linearized pAdAChET in 293 cells and 4×1010efu/mL titer of Ad AChET was obtained by CsCI gradient purification.
     经293细胞包装,3 d后观察到绿色荧光蛋白(GFP)明显表达,氯化铯梯度离心纯化最终获得约4×1013efu/L滴度的重组病毒;
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     GFP expression could be observed 3 d after packing of the linearized pAd-E6E7 in 293 cells and 6.9×10~ 10 pfu/L titer of Ad E6E7 was obtained by CsCl gradient purification.
     经人胚肾293细胞包装,3d后观察到绿色荧光蛋白(GFP)明显表达,氯化铯梯度离心纯化最终获得6.9×1010pfu/L滴度的重组病毒;
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  “expression could”译为未确定词的双语例句
     Mutated P53 expression could be observed in SCC(80.0%),BCE(75.0%),BD(33.3%),but not normal tissue and SK(13.3%).
     突变型P53在SCC(80.0%),BCE(75.0%),BD(33.3%)肿瘤组织的阳性率较高,而在SK(13.3%)中基本不表达。
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     VEGF protein expression could be observed in SCC(85.0%),BD(60.0%),SK(53.3%),but not significant in normal tissue and BCE(13.3%).
     VEGF在SCC(85.0%),BD(60.0%),SK(53.3%)的表达阳性率较高,而在正常组织和BCE(13.3%)中基本不表达.
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     Results Maternal serum sFlt-1 was significantly higher in preeclampsia group than control, (16.9±8.32) μg/L vs (5.48±2.09)μg/L, P<0.05.The sFlt-1 mRNA expression could be detected in both groups.
     结果外周血中sFlt-1的水平子痫前期组明显高于对照组,分别为(16.9±8.32)μg/L、(5.48±2.09)μg/L,两者差异有显著性(P<0.05)。
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     While IgH and (or) Vδ 2Dδ 3 turned negative, mdr 1 gene expression could be a supplementary indicator of predicting relapse.
     当IgH或 /和Vδ2 Dδ3 转阴后检测mdr 1基因表达可作为预知复发的一个辅助指标
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     HBV X expression could up-regulate p53 gene expression level induced by As_2O_3,while it suppressed the activity of p53.
     HBX表达上调As_2O_3诱导的p53蛋白水平,但显著抑制p53的结合与转录调节活性。
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     Expression of E.
     将阳性重组质粒转化表达受体菌E.
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     Its activity could be regulated by the expression of its subunit.
     端粒酶的激活可能与其亚单位特别是端粒酶活性催化亚单位的表达、调控有关。
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     Taxol could decrease the cyclinE expression.
     泰素作用MKN-45细胞24h后cyclinE表达下调,cyclinD1表达基本不变;
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     It is the expression of subjectivity.
     好是主体的私意的表达。
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  expression could
The decrease in the level of Six3 expression could be due to antagonistic interrelations of Prox1 and Six3.
      
Its level at stages of 15 to 24 weeks was very low, and no expression could be detected in 27-to 28-week fetuses.
      
High-level expression could be obtained by synthesizing a codon-optimized ST gene and expressing it in the form of inclusion bodies.
      
No expression could be detected until three days after germination, and its peak was registered at the fifth day after germination.
      
For example, adhesion to the urethral and bladder mucosa could be facilitated by pili and their expression could be advantageous in the early phase of prostatitis.
      
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In the present paper the plastic part of the J-integral was derived on the base of the model of pure power hardening materials. The new expression found was J_p=-(U_p)/B((1/p )((?)p) /((?) q)) An attempt was also made to elucidate the thoretical basis and the approximity nature of the expression in current useJ_p =2U_p/B(w-α)· This later expression could be arrived at by assuming (1/p ((?)p) /((?) α)_△≈(1/pL ((?)pL) /((?) α)) and substituting it in the new expression.In this paper J-integral tesistance...

In the present paper the plastic part of the J-integral was derived on the base of the model of pure power hardening materials. The new expression found was J_p=-(U_p)/B((1/p )((?)p) /((?) q)) An attempt was also made to elucidate the thoretical basis and the approximity nature of the expression in current useJ_p =2U_p/B(w-α)· This later expression could be arrived at by assuming (1/p ((?)p) /((?) α)_△≈(1/pL ((?)pL) /((?) α)) and substituting it in the new expression.In this paper J-integral tesistance curves of four kinds of materials were quoted from our laboratory. It was found from the curves that: (1) within a quite wide range of the specimen sizes the plane strain resistance curve seems to be an inherent characteristic of the materials; (2) to the composition, heat treatment and microstructutre of the materials tested the crack initiation toughness is not so sensitive as the resistance curve; (3) for predicting K_(?) of large specimen with the aid of resistance curve, different points thn should be used for the two types of materials. For one type, K_(?) is related to the highest point (saturation point), for the other type to the initial point of the resistance curve.

本文根据纯幂硬化材料模型导出了J积分的塑性部分,又根据的假定,试图说明常用的Jp=2Up/B(W-a)计算式的理论根据和近似性。本文搜集了本实验室对四种材料所散的J积分阻力曲线,结果表明:(1)在相当大的试样尺寸变化范围内,阻力曲线近似为材料特性(2)某些材料的起裂韧性对成分,热处理和微观组织结构不敏感,而阻力曲线则不然(3)用阻力曲线预言大试样K_(IC)时,要区分两种类型材料,其K_(IC)分别相应阻力曲线最高点(饱和点)和起裂点。

Using methods of nucleic acid lybridization In Situ and ultrasonicsplitted gene fragment, this paper reported detections of EBV gene fragments in tumor cells of NPC and Burkitt's lymphoma and host cells transfrred by EBV gene and anal-ysis of gene expression.The experimental results showed that there were three events In Vitro (1) During the latent existence the EBV-RNA transcription and EBNA expression could be demonstrated by molecular lybridazation In Situ; (2) After the EBV gene transfer, the homogenous...

Using methods of nucleic acid lybridization In Situ and ultrasonicsplitted gene fragment, this paper reported detections of EBV gene fragments in tumor cells of NPC and Burkitt's lymphoma and host cells transfrred by EBV gene and anal-ysis of gene expression.The experimental results showed that there were three events In Vitro (1) During the latent existence the EBV-RNA transcription and EBNA expression could be demonstrated by molecular lybridazation In Situ; (2) After the EBV gene transfer, the homogenous base squences of EBV-DNA “W” fragment (Bam HI W/Sau Ⅲ a) had inserted in and localized on host cell DNA; (3) The study also found the closed correlation among the EBV-RNA transcription, the EBNA expression and the transformed cell phenotype by cellular agglutination.This study provided a new thought and some approaches for the mechanism on malignant transformation of host cell. It is worthy of further study whether the EBV-DNA “W” segment is the transforming fragment or not.

本文应用原位核酸分子杂交定位和超声切割基因片段技术,对鼻咽癌、Burkitt淋巴瘤细胞和EBV基因转移细胞进行基因片段探测,以及基因表达状态的分析。实验结果表明:(1)EBV基因的潜伏(隐匿)和再激活开放过程中,EBV-RNA的转录和EBNA的表达相关性,可以应用原位核酸分子杂交法显示和定位,(2)EBV基因转移后,与EBV-W片段的同源顺序,已插入和定位于细胞DNA中;(3)EBV基因转移,EBV-RNA的出现与EBNA产生相关;EBNA的表达,又与细胞转化表型——细胞凝集相关。 EBV基因片段的转移,EBV-RNA的转录、基因产物-EBNA的表达以及细胞凝集转化表型的出现,这一检测系统对细胞恶性转化机制提供了新的技术途径和思路。 EBV-“W”片段(BamHI W/Sau IIIa)作为转化片段的设想值得进一步研究。

In Ref[1], a damage-level function is proposed for analyzing the dynamic fracture processes of expanding cylindrical shell. In this paper, we extend the model to the case of planar geometry and perform numerical simulation. for the dynamic damage processes under uniaxial strain case.The studied material is LY-12 aluminum and a kind of constitutive equation with viscous effect is utilized. The calculated free-surface velocity versue time relation is consistent with the experimental results. The calculated results...

In Ref[1], a damage-level function is proposed for analyzing the dynamic fracture processes of expanding cylindrical shell. In this paper, we extend the model to the case of planar geometry and perform numerical simulation. for the dynamic damage processes under uniaxial strain case.The studied material is LY-12 aluminum and a kind of constitutive equation with viscous effect is utilized. The calculated free-surface velocity versue time relation is consistent with the experimental results. The calculated results also indicate that the spall strength ac and the critical damage-level at fracture plane, ac, are both monotone increase function of strain rate ec, respectively. This kind of ac ~ec relation has been reported in many other articles, for example in Ref[2,3].The relation ac-ec can be expressed as ecexp[ -11.4ac] =2100s-1 in the strain-rate range from 105s-1 to 106s-1.We think this expression could be used as a.kind of spall criterion.The damage-level profiles in the spalled layer are also given from the numerical results, and the specific feature of the profiles are qualitatively in accord with that of the microphotographic results from recovery specimens reporded by Barbee, et al.

本文从文献〔1〕中用于分析柱壳动态膨胀断裂过程的损伤度函数出发,将它推广到对一维应变下层裂过程的数值模拟研究。试件材料为LY-12铝,其特性方程取为含粘性的本构方程形式。数值计算结果很好地再现了实测自由面速度u_(f5)随时间t的变化过程,并表现出层裂强度σ_c及层裂面上的临界损伤度α_c都分别是应变率ε_c的单调递增函数关系。σ_c~ε_c的这种变化规律在许多文献中已屡见报导,例如可见文献〔2,3〕。在10~5S~(-1)-10~6 S~(-1)应变率范围内,α-c~ε_c关系可以表示为ε_c exp(-11.4α_c)=2100S~(-1),这个式子可以做为一种层裂判据使用。数值计算还给出了层裂片内的损伤度削面,其形状特征与Barbee等对回收试件的细观测量结果在定性上一致

 
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