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terminus
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  5′端
     Methods An complementary sequence of NcoI site and a seqence coding for MG7-Ag mimotope was designed at the 5' terminus of forward primer.
     方法将NcoⅠ位点和MG7-Ag模拟表位编码序列的互补序列设计到上游引物的5′端
短句来源
     The eukaryotic expression plasmid pEGFP-N1/DPF-1 was constructed through fusing rabbit oviduct-specific glycoprotein (DPF-1) gene to the 5' terminus of eGFP gene. After transfecting the recombinant plasmid into HeLa cells, we got some cell strains expressing and secreting eGFP-DPF-1 stably.
     将兔输卵管蛋白(DPF-1)基因连结于增强型绿色荧光蛋白(eGFP)基因5′端,构建了真核表达重组质粒(pEGFP-N1/DPF-1),转染HeLa细胞,获得稳定表达分泌融合蛋白eGFP-DPF-1的HeLa细胞株。
短句来源
     In one way, a secretion signal peptide (SG, 139bp) derived from a mouse IgH chain was introduced into the 5' terminus of
     第二种修饰是除在5′端引入信号肽序列,还在VP4基因3′端引入来自人HLA Ⅰ类分子α链的3′端部分TM(750bp),记为SG-VP4-TM,目的是使获得糖基化的VP4向胞外分泌,并停留于细胞膜,拟提高免疫原性。
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  “5 ' terminus”译为未确定词的双语例句
     Expression of B and C Antigenic Sites of TGEV Spike Gene in Prokaryocyte and Preparation of Nucleic Acid Probe at 5' Terminus
     TGEV S基因B和C抗原位点片段的原核表达及5’核酸探针制备
短句来源
     The fragment containing antigenic sites B and C locating at the 5' terminus in spike gene of TGEV was deleted in PRCV. The 1st pair of primer was designed according to strain TH-98.The fragment (named ts) was amplified by RT-PCR and inserted into pMD18-T vector.
     根据GenBank TH-98株序列设计第1对引物,应用RT-PCR方法体外扩增TGEV TH-98株S基因5’端含有B、C抗原位点的片段(ts),大小为357bp,并将ts连接到pMD18-T载体上。
短句来源
     The VH and Vk genes amplified by addingon PCR,as well as a part of linker peptide gene sequence was added on VH 3' and Vk 5' terminus respectively.
     用加端PCR分别在VH基因3'端和Vk基因5'端延伸部分连接肽基因序列,回收后混合运火;
短句来源
     The plasmid pET22-ScFv-PE40 was sepuenced to select the plasmid in which 5' terminus of the PE40gene follows 3' terminus of the ScFv gene. Recombinant gene expression was induced by the addition of IPTG for five hours.
     测序鉴定插入方向,选取以PE40基因5'端与ScFv基因3'端连接的重组质粒pET22-ScFv-PE40。
短句来源
     The 5'terminus of heat-labile was fused to the 3'terminus of k99 Fimbrial antigen gene by PCR.
     利用PCR技术从原始菌种中扩增得到K99和LTB基因,将K99和LTB基因连接起来,构建了LTB-K99融合基因。
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  相似匹配句对
     (5) O.
     (5)尖叶棘豆O.
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     (5).
     5.商贾家训
短句来源
     The 5'-terminus ~(82)P labeledproduct is verified by sequence analysis.
     产物的5′-OH用~(32)P标记后,同系层析均一,蛇毒磷酸二酯酶部分酶解后,作电泳-同系层析双向图谱,证明核苷酸排列顺序正确。
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  terminus
The 5' terminus of the p19 subgenomic RNA was identified at position 2598 and an essential transcription signal for this mRNA mapped between positions 2534 and 2563.
      
The LChV-2/USA6b genome is potentially ambi-sense, with a negative sense ORF0 at the 5' terminus, from which an 18.1?kDa protein of unknown function can be expressed in vitro.
      
The two small open reading frames (VP5) besides the 5' terminus of the VP2 gene were found on segment A of YAV.
      
The addition of a "G" residue was found at the 5' terminus of all 8 Japanese isolates.
      
Variation within a 523 nucleotide region proximal to the 5' terminus of seven rubella virus strains has been anlysed.
      
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A new colorimetrio method has been devised for the determinaton of 3'-termini of oligoribonucleotides. This method is based on the principle that the terminal ribosyl moieties of oligoribonucleotides are oxidized to the corresponding dialdehydos which react with certain colored hydrazides to produce the terminal labeled oligoribonucleotide hydrazones.In this report, the methods for the preparation of colored hydrazides, 4-methylaminoazobenzene-4'-sulphonyl glycyl hydrazide(DABS-Gly-NHNH_2)and 4-dimothyl-aminoazobenzene-4'-sulphonyl...

A new colorimetrio method has been devised for the determinaton of 3'-termini of oligoribonucleotides. This method is based on the principle that the terminal ribosyl moieties of oligoribonucleotides are oxidized to the corresponding dialdehydos which react with certain colored hydrazides to produce the terminal labeled oligoribonucleotide hydrazones.In this report, the methods for the preparation of colored hydrazides, 4-methylaminoazobenzene-4'-sulphonyl glycyl hydrazide(DABS-Gly-NHNH_2)and 4-dimothyl-aminoazobenzene-4'-sulphonyl hydrazide (DABS-NHNH_2) as well as their nuclooside hydrazones are presented.These hydrazones are hydrolyzed with nueleases and the nucleosido hydrazones released can be visualized directly on the polyamide thin layer plate after spraying hydrochloric acid.The sensitivity of this method may be indicated by the fact that as little as 10~(-9) 10~(-10) mole of nucleosides can be labeled and detected on the polyamide plate.Reagent DABS-Gly-NHNH_2 has been employed to label the 3'-termini of four different oligoribonucleotides, the amount of which ranged from 0.08~0.12 A at 260 nm. (about 10~(-9) mole).The capacity of the two types of hydrazides for labeling the nucleoside dialdehydes is briefly discussed.

本文报告用甲基橙为原料合成了甲基橙的两种衍生物:4-二甲氨基偶氮苯-4'-磺酰-甘氨酰肼(DABS-Gly-NHNH_2,Ⅰ)和4-二甲氨基偶氮苯-4'-磺酰肼(DABS-NHNH_2,Ⅱ),并用(Ⅰ)成功地标记了高碘酸氧化的核苷二醛。标记产物在聚酰胺薄板上分离得很好,其灵敏度达到10~(-9)~10~(-10)克分子,比紫外吸收法灵敏数十倍。我们用试剂(Ⅰ)标记测定了四种不同的寡核糖核苷酸的3'-端,用量为0.08~0.12A_(260)。

This paper describes the purification method of yeast alanine tRNA with DEAESephadex A-25 column chromatography and two half molecules of the tRNA~(ala) were identified. Its purity was 60~70%. The yeast tRNA~(ala) was digested with 15 units of RNase T_1 per mg tRNA for 4 min. At 0℃, and two half molecules resulted were separated by DEAE-cellulose column chromatography. The 3'-terminus of the 5'-half molecule obtained was identified as guanylio acid by fluorescent labeling and [~3H]-labeling methods, and...

This paper describes the purification method of yeast alanine tRNA with DEAESephadex A-25 column chromatography and two half molecules of the tRNA~(ala) were identified. Its purity was 60~70%. The yeast tRNA~(ala) was digested with 15 units of RNase T_1 per mg tRNA for 4 min. At 0℃, and two half molecules resulted were separated by DEAE-cellulose column chromatography. The 3'-terminus of the 5'-half molecule obtained was identified as guanylio acid by fluorescent labeling and [~3H]-labeling methods, and the 5'-terminus of the 3'-half molecule as cytidylic acid by [~(32)p]-labeling method. Therefore, these results confirmed that cleavage site of tRNA~(ala) by limited digestion with RNase T_1 is at G-C link in the anticodon.

本文报道了用DEAE-葡聚糖凝胶A-25柱层析纯化酵母丙氨酸tRNA的方法,纯化的tRNA,按接受丙氨酸的活力计算,其纯度达60~70%。经核糖核酸酶T_1(RNase T_1)限制性降解(tRNA与RNase T_1的比率为1毫克/15单位,0℃,4分钟),柱层析,制备了tRNA的两个半分子。用萤光标记法,[~3H]标记法,测得5′半分子的3′末端为鸟苷酸;用[~(32)P]标记法测得3′半分子的5′末端为胞苷酸。因此,RNase T_1限制性降解丙氨酸tRNA的切点在反密码子的G—C键之间。

This paper describes the purification method of yeast alanine tRNA with DEAE-Sephadex A-25 column chromatography and two half molecules of the tRNA~(ala) wereidentified. Its purity was 60~70%. The yeast tRNA(ala) was digested with 15 unitsof RNase T_1 per mg tRNA for 4 min. At 0℃, and two half molecules resulted wereseparated by DEAE-cellulose column chromatography. The 3′-terminus of the 5′-halfmolecule obtained was identified as guanylic acid by fluorescent labeling and[~3H]-labeling methods, and the...

This paper describes the purification method of yeast alanine tRNA with DEAE-Sephadex A-25 column chromatography and two half molecules of the tRNA~(ala) wereidentified. Its purity was 60~70%. The yeast tRNA(ala) was digested with 15 unitsof RNase T_1 per mg tRNA for 4 min. At 0℃, and two half molecules resulted wereseparated by DEAE-cellulose column chromatography. The 3′-terminus of the 5′-halfmolecule obtained was identified as guanylic acid by fluorescent labeling and[~3H]-labeling methods, and the 5′-terminus of the 3′-half molecule as cytidylic acidby [~(32)p]-labeling method. Therefore, these results confirmed that cleavage site oftRNA~(ala) by limited digestion with RNase T_1 is at G-C link in the anticodon.

本文报道了用DEAE-葡聚糖凝胶A-25柱层析纯化酵母丙氨酸tRNA 的方法,纯化的tRNA,按接受丙氨酸的活力计算,其纯度达60~70%。经核糖核酸酶T_1(RNase T_1)限制性降解(tRNA 与RNase T_1的比率为1毫克/15单位,0℃,4分钟),柱层析,制备了tRNA 的两个半分子。用萤光标记法,[~3H]标记法,测得5′半分子的3′末端为鸟苷酸;用[~(32)P]标记法测得3′半分子的5′末端为胞苷酸。因此,RNase T_1限制性降解丙氨酸tRNA 的切点在反密码子的G—C 键之间。

 
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