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eight-cell
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  8-细胞
     Survival of Two-step Open-pulled Straw Vitrification of Mouse Eight-cell Embryos
     OPS两步法玻璃化冷冻小鼠8-细胞胚胎生存发育率的研究
短句来源
     Methods The experiments were conducted at 37℃ hot plate and 25℃ room temperature to cryopreserve mouse eight-cell embryos with EDFS30 (an ethylene glycol-based vitrification solution) in Open-pulled Straw (OPS).
     方法以小鼠8-细胞胚胎为材料,在25℃室温和37℃恒温台条件下,利用玻璃化冷冻溶液EDFS30, 对小鼠8-细胞胚胎进行玻璃化冷冻保存,解冻后培养60 h后的囊胚发育率为其体外发育能力的考核指标,同时对解冻后培养1-3 h的胚胎进行移植以判定其体内发育潜力。
短句来源
     Results The higher blastocyst rates of vitrified embryos were 95.6%, which were similar (P>0.05) to that (93.0%) of control. The embryos derived from the best vitrified group or fresh eight-cell embryos (used as control) after culture for 1 to 3h were transferred to 63 to 67h pseudo-pregnant female mice.
     结果小鼠胚胎8-细胞冷冻后囊胚发育率最佳组高达95.6%,与对照组(96.8%)差异无统计学意义(P> 0.05)。
短句来源
     Conclusion The mouse eight-cell embryos could survive cryopreservation by vitrification with EDFS30 in OPS.
     结论 EDFS33为玻璃化冷冻液,OPS两步法能有效地冷冻保存小鼠8-细胞胚胎。
短句来源
  “eight-cell”译为未确定词的双语例句
     Then the cell was inserted into the perivitelline space of the enucleated porcine oocyte to investigate the development of interspecies embryo. Results All the non-transgenic NIH3T3 group,transgenic Oct4-EGFP NIH3T3 and pEGFP-N-1 groups could support the early development of cloned interspecies embryo,and the cleavage rate and development rate into the eight-cell stage were not significantly different.
     结果未转染的NIH3T3阴性对照组、转染Oct4-EGFP的小鼠NIH3T3细胞和转染pEGFP-N1组均能够支持猪异种核移植胚胎的早期发育,但转染pEGFP-N1-Oct4-EGFP实验组重构胚的卵裂率和8细胞发育率与转染pEGFP-N1组和未转染的NIH3T3组的重构胚发育率差异不显著。
短句来源
     The proportion of eight-cell embryos and blastocysts in 10ng/ml P group(54.7%;18.9%)were significantly higher than those of the control(39.0%;9.6%)(P<0.01;P<0.05).
     其中,10ng/ml孕酮添加组的8细胞胚胎发生率(55%)极显著高于对照组(39.0%)(P<0.01); 囊胚发生率(18.9%)亦显著高于对照组(9.6%)(P<0.05)。
短句来源
     progesterone level was strongly correlative. with the number of three to eight-cell cmbryos(r_3 = 0.6692,P<0.01).
     与2细胞以上胚胎数(不包括2细胞)均表现出强正相关(r_3=0.6692,P<0.01)。
短句来源
     To investigate the expression patterns of histone acetyltransferase (GCN5) and historic deacetylase 1 (HDAC1) in preimplantation mouse embryos and the effects of in-vitro cultures on their expressions, immunocytochemistry was used to detect the expressions of GCN5 and HDAC1 in mouse embryos at the stages of two-cell, four-cell, eight-cell, morula and blastocyst in both in-vivo and in-vitro groups.
     为探讨小鼠植入前胚胎组蛋白乙酰化酶GCN5(general control of nucleotide synthesis,GCN5) 和组蛋白去乙酰化酶1(histone deacetyluse1,HDAC1)的表达模式及常规体外培养对它们表达的影响,应用荧光免疫细胞化学技术,检测了体内和体外培养的小鼠2、4、8细胞期卵裂胚胎、桑葚胚和囊胚GCN5和HDAC1的表达。
短句来源
     In vitro matured bovine oocytes, two-cell and eight-cell embryos, and day-9 hatched blastocysts subjected to control and elevated temperature conditions were analyzed by semi-quantitative reverse transcription polymerase chain reaction methods for hsp70 mRNA expression.
     Hsp70mRNA半定量RT-PCR分析证明,体外培养的早期牛胚通过增强基因表达,有能力对热应激产生应答。 2细胞胚、8细胞胚及第9天的囊胚接触4222热应激4h后,可通过转录hsp70基因,对热应激产生应答,随着胚胎发育的进展,应答反应增强。
短句来源
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  相似匹配句对
     Eight warm
     8·温暖
短句来源
     Eight species, D.
     boryanum ,陕甘介蕨D .
短句来源
     Study on eight extra-meridians
     奇经八脉探讨
短句来源
     Results Twenty-eight strains Y.
     结果 检出Y .
短句来源
     Cytotoxic T Cell
     细胞毒T细胞
短句来源
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  eight-cell
Human pregnancies after transfer of fresh (four-to eight-cell) versus frozen-thawed blastocysts resulting from intracytoplasmic
      
We examined whether cryopreserved mouse eight-cell embryos could be thawed, biopsied, refrozen, thawed, and grown in vitro and in vivo in two sets of experiments.
      
The use of acidic Tyrode's solution for clinical assisted hatching of eight-cell embryos is currently under investigation.
      
Cryopreservation resulted in a longer delay in cleavage from the four-to the eight-cell stage of S (about 5 hr)- and G2-phase embryos (about 3 hr) compared to unfrozen controls.
      
(2) Oxygen consumption analysis revealed that cultured blastocysts actively respired at a level close to that observed with freshly recovered eight-cell embryos (slopes of oxygrams: 0.25 and 0.26, respectively).
      
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Blastomeres of the two, four and eight cell stage as well as embryos of the gastrula stage of Bufo melanostictus were irradiated with ruby laser microbeam of focusing diameter 10μ, and pulse duration lusec. After irradiation, a damage spot or a mushroom-like swelling would appear on the surface of the egg. It was found that the mortality of the embryos was closely related to energy densities of the laser microbeam used, and also the earlier tne stage of embryonic development was irradiated, the higher the mortality....

Blastomeres of the two, four and eight cell stage as well as embryos of the gastrula stage of Bufo melanostictus were irradiated with ruby laser microbeam of focusing diameter 10μ, and pulse duration lusec. After irradiation, a damage spot or a mushroom-like swelling would appear on the surface of the egg. It was found that the mortality of the embryos was closely related to energy densities of the laser microbeam used, and also the earlier tne stage of embryonic development was irradiated, the higher the mortality. In the two cell stage, when the energy density was below 312uJ/μm~2, no conspicuous external change was observed.The mortality of the embryos increased as the energy density was raised from 312uJ/μm~2 to 623 uJ/μm~2. However, all the embryos died early when the energy density was raised to 1244uJ/μm~2. Various abnormalities appeared on the embryos or tadpoles, such as, a white patch lacking ectoderm on the body wall, curved tail,a small undeveloped head, smaller tadpoles with abdominal edema and shorter intestine etc. The majority of the enbryos which had been irradiated died in between the tail-bud stage and the closure of the operculum, and only a few embryos could develope to metamorphosis. When the cleavage furrow of the two cell stage was irradiated with an energy density of 623μJ/μm~2, most of the embryos died in the gastrula stage.When the blastomcres of the two cell stage of Bufo melanostictus and Rana limnocharis were irradiated with the same energy density and compared, the mortality of the embryos of the former species was much higher than that of the latter. Since the eggs of Bufo melanostictus are much darker than those of Rana.limnocharis, it is evident that the dark pigmented cortex of the eggs of Bufo melanostictus can enhance the effect of the laser microbeam.

用红宝石激光微束(聚焦光斑直径10μ)照射黑眶蟾蜍二、四、八细胞期的分裂球和原肠胚,产生的畸形胚胎和死亡率,可因发育时期不同和照射能量密度的大小有所差异。发育时期愈早,其死亡率愈高。在二细胞期,当照射能量密度低于312μJ/μm~2时。观察不出有显著的外部变化。照射能量密度提高到312、470或623μJ/μm~2时,胚胎死亡率亦随之增高;但用1244μJ/μm~2的能量密度的光束照射,全部胚胎死亡。正在分裂的卵被照射后,发育成为各样的畸形胚胎或蝌蚪而死亡,只少数能够发育至变态成小蟾蜍。用623μJμm~2能量密度照射二细胞期卵裂沟的卵,多数只能发育至原肠胚,少数发育成为头部分化不全的蝌蚪而死亡。用相同能量密度照射黑眶蟾蜍和泽蛙的二细胞期分裂球,由于黑眶蟾蜍卵皮层的色素较泽蛙的黑,增强了激光微束照射的效应,致使死亡率明显地提高。

The paper describes the experimental results in the following three aspects. 1. The structure of pistil and ovule: The pistil of Hevea consists of three carpels. The transmitting tissue in style can be seen clearly. The ovary is made up of three chambers, each containing only one anatropous ovule. There is an obturator covering the microphyle. The ovule is crassinucellate because the nucellus, with an obvious necellar beak, is massive. The embryo sac is com- posed of eight cells. 2. The development and deterioration...

The paper describes the experimental results in the following three aspects. 1. The structure of pistil and ovule: The pistil of Hevea consists of three carpels. The transmitting tissue in style can be seen clearly. The ovary is made up of three chambers, each containing only one anatropous ovule. There is an obturator covering the microphyle. The ovule is crassinucellate because the nucellus, with an obvious necellar beak, is massive. The embryo sac is com- posed of eight cells. 2. The development and deterioration of endosperm and the formation of prosembryum: The mitosis of the primary endosperm nucleus takes place for the first time 24 hours after pollination, but the division of the cytoplasm does not occur. The free nuclei surrounding the embryo begin to form cell walls 60-65 days after pollination, and all of them become endosperm cells 70 days after pollination. The nutrients of endosperm cells are gradually absorb- ed by the developing embryo and nucellus and, as a result, the cells are red- uced to nothing but a thin layer of dead tissue surrounding the whole embryo. However, the nucellus becomes thicker and thicker and eventually constitutes the major portion of the mature seed and forms the permanent food storing tissue called prosembryum. 3. The development of embryo: The transverse mitosis of zygote occurs about 40 days after pollination. Later the apical cell undergoes longitudinal divisions. Then follows a series of diagonal divisions resulting in the forma- tion of a young embryo possessing the embryo bud initial cells. With the devel- opment of the apical cell,the basal cell undergoes transverse division to form two cells: the upper one forms the single cell suspensor; the lower one Will form the root cap. The development of the embryo in Hevea is of the crucifer type.

本文分三方面叙述。 1.雌蕊及胚珠的构造:巴西橡胶花的雌蕊由三心皮构成。花柱中的诱导组织明显。子房三室,每室仅有一枚倒生胚珠。珠孔上方罩有一帽状珠孔塞。珠心发达,属厚珠心型,有明显的珠心喙。胚囊由8个细胞组成。 2.胚乳的发育、消退和外胚乳的形成过程:初生胚乳核在授粉后24小时开始进行第一次有丝分裂,授粉后60—65天珠孔端的游离核开始产生细胞壁,到授粉后70天左右,全部的游离核都形成了胚乳细胞。以后胚乳细胞的养分逐渐被发育中的幼胚和珠心所吸收,最后只剩下极薄的一层。而珠心组织则一天天增厚,其细胞体积也一天天增大,最后成为种子的永久性的营业养组织即外胚乳。 3.胚的发育过程:合子横分裂是在授粉后40天左右。接着顶细胞进行纵裂,随后胚体细胞以对角线的方式进行分裂产生一个具胚芽原的幼胚。随着顶细胞的发育,基细胞也进行横分裂产生上下两个细胞,上方一个体积略为增大而形成胚柄;下方一个将来发育成根冠。因此巴西橡胶树的胚属十字花型。

VHA-273 and HA-MEV are two strains of multiple-embedded NPVs of different origins of the Chinese cotton ball-worm Heliothis armigera. The former was obtained from Hubei Province, China, while the latter came from U. S. S. R. In order to explore the susceptibility of the cell lines for establishing a superior virus-cell system, eight cell lines of seven lepidopteran insects have been used to infect with these two virus strains. They are as following: BCIRL-HA-AM1 of Heliothis armigera, BCIRL-HZ-AM3 of H. zea,...

VHA-273 and HA-MEV are two strains of multiple-embedded NPVs of different origins of the Chinese cotton ball-worm Heliothis armigera. The former was obtained from Hubei Province, China, while the latter came from U. S. S. R. In order to explore the susceptibility of the cell lines for establishing a superior virus-cell system, eight cell lines of seven lepidopteran insects have been used to infect with these two virus strains. They are as following: BCIRL-HA-AM1 of Heliothis armigera, BCIRL-HZ-AM3 of H. zea, BCIRL-HV-AM2 of H.virescens, BCIRL-SO4-HNU2 and BCIRL-SO3-HNU1 of Spodoptera orinthogalle, IPLB-SF-21 of S. frugiperda, TN-368 (CL1) of Trichoplusia ni, and BCIRL-PX2-HNU3 of Plutella xylostella. In addition, VHA-273 has been used to infect another cell line BCIRL-HS4-HNU4 of cottonworm. Heliothis subflexa.

VHA-273和HA-MEV是两个不同来源的中国棉铃虫多粒包埋核型多角体病毒毒株。前者是从我国湖北省荆州地区获得。后者最初来自苏联。为了探寻对这些病毒敏感的细胞系,建立起较优的病毒——细胞体系,用这两个毒株分别感染了七种鳞翅目昆虫的八个细胞系。这些细胞是中国棉铃虫(Heliothis armigera)的BCIRL-HA-AM1、美国棉铃虫(H.zea)的BCIRL—HZ—AM3、烟夜蛾(H.virescens)的BCIRL—HV—AM2、黄条行军虫(Spodoptera ornithogalle)的BCIRL—SO3—HNU1与BCIRL—SO4—HNU2、草地贪夜蛾(S.frugiperda)的IPLB—SF—21、粉纹夜蛾(Trichoplusia ni)的TN—368(CLI)和小菜蛾。(Plutella xylostella)的BCIRL—PX2—HNU3。此外还用VHA—273感染过另一种实夜蛾(H.Subflexa)的BCIRL—HS4—HNU4。病毒接种物包括稀释的病虫血淋巴以及具有感染性的细胞培养基。不论是病虫血淋巴或具有感染性的培养基都能使敏感细胞受染。病毒的复制以培养的细胞在细胞核中出现...

VHA-273和HA-MEV是两个不同来源的中国棉铃虫多粒包埋核型多角体病毒毒株。前者是从我国湖北省荆州地区获得。后者最初来自苏联。为了探寻对这些病毒敏感的细胞系,建立起较优的病毒——细胞体系,用这两个毒株分别感染了七种鳞翅目昆虫的八个细胞系。这些细胞是中国棉铃虫(Heliothis armigera)的BCIRL-HA-AM1、美国棉铃虫(H.zea)的BCIRL—HZ—AM3、烟夜蛾(H.virescens)的BCIRL—HV—AM2、黄条行军虫(Spodoptera ornithogalle)的BCIRL—SO3—HNU1与BCIRL—SO4—HNU2、草地贪夜蛾(S.frugiperda)的IPLB—SF—21、粉纹夜蛾(Trichoplusia ni)的TN—368(CLI)和小菜蛾。(Plutella xylostella)的BCIRL—PX2—HNU3。此外还用VHA—273感染过另一种实夜蛾(H.Subflexa)的BCIRL—HS4—HNU4。病毒接种物包括稀释的病虫血淋巴以及具有感染性的细胞培养基。不论是病虫血淋巴或具有感染性的培养基都能使敏感细胞受染。病毒的复制以培养的细胞在细胞核中出现病毒多角体为标准。发展最快的,在接毒后三天,核内即可见到多角体,但大多数在一周左右。试验结果表明:对VHA—273敏感的包括粉纹夜蛾、草地贪夜蛾和小菜蛾的三种细胞系,对HA—MEV敏感的有草地贪夜蛾和粉纹夜蛾的二种细胞系。其他各种受试细胞对这些毒株敏感性很低或不敏感。将已复制多角体后的细胞用超声细胞破碎器处理以释出多角体,三次重复计数的结果为:VHA—273在粉纹夜蛾、草地贪夜蛾、小菜蛾细胞内复制量分别为13×10~5PIB_s/ml、11×10~5PIB_s/ml、2.7×10~5PIB_s/ml;HA—MEV在草地贪夜蛾、粉纹夜蛾细胞内复制的量分别为3.8×10~5PIB_s/ml、3.5×10~5PIB_s/ml。用细胞培养复制出的病毒多角体稀释成不向浓度,感染对本病毒敏感的美国棉铃虫的初孵幼虫,每种浓度试验用虫25—50头,其致死中浓度为VHA—273:830PIB_s/cm~2,HA—MEV:478PIB_s/cm~2。当浓度为1000PIB_s/cm~2时,VHA—273与HA—MEV感染幼虫的死亡率分别为74%和82%,浓度为5000PIB_s/cm~2时,其死亡率均达92%。

 
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