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conventional rats
相关语句
  普通级大鼠
     Comparison on Some Biologic Properties of SPF and Clean and Conventional Rats
     SPF、清洁及普通级大鼠部分生物学特性的比较
短句来源
     M. pulmonis infcetion was found in 91% of 130 conventional rats tested.
     在所检测的130只普通级大鼠中,抗体阳性率达91%。
短句来源
     Bythis method, it was found that the antibody positive rate of SPF rats maintained inisolators was 0 (0/28), while that of conventional rats maintained in open systemwas 40.9% (34/88), indicating different infective rates of rats in different envi-ronments. The antibody positive rate of conventional mice maintained in the sameopen system was only 3%.
     用该法检测不同饲养环境中的大鼠,结果:饲养在隔离器内的SPF级大鼠的抗体阳性率为0(0/28),饲养在开放系统内的普通级大鼠的抗体阳性率为40.9%(36/88),而饲养在同一开放系统内的普通级小鼠的阳性率仅为3%。
短句来源
  “conventional rats”译为未确定词的双语例句
     Study on Gastroelectric Activity and Gastric Motility in Germ Free Rats and Conventional Rats
     无菌Wistar大鼠胃电与胃运动的观察
短句来源
     This Kit was used in rountine monitoring. The results indicated that the infection rate was 38.2% (55/114) in conventional rats and 0% (0/10) in clear rats.
     并用本试剂盒对154只大鼠的PVM抗体水平进行调查,结果是开放大鼠PVM阳性率为38.2%,清洁大鼠为0。
短句来源
  相似匹配句对
     The conventional R.
     传统R.
短句来源
     CHANGES OF CONVENTIONAL NUTRIENT METABOLISM IN BURNED RATS
     烧伤大鼠常规营养物质代谢的变化
短句来源
     Rats in the normal group were fed with conventional food.
     正常组:常规基础饲料膳食。
短句来源
     with the conventional therapy.
     笔者认为用MEBO治疗隐翅虫皮炎是一种值得推广的方法。
短句来源
     All rats
     ②手术对照组:给大鼠施予与实验组大鼠相同
短句来源
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  conventional rats
In contrast, germ-free rats exhibited relatively high levels of β-D-glucosidase activity (about 80% of total activity in the conventional rats) in the proximal small intestine (PSI) and the distal small intestine (DSI).
      
Enzymatic activity (β-D-glucosidase and β-D-glucuronidase) was greatest in the lumen of cecum and colon of conventional rats.
      
Nephrectomy increased brain histamine and 5-hydroxyindoleacetic acid levels in both germ-free and conventional rats, but had no effect on norepinephrine, dopamine or serotonin.
      
For germ-free rats the increase of non-acid glycolipids was ascribed to an effect of the lectin strictly on the small intestinal mucosa, while for conventional rats an effect was seen also on the large intestinal mucosa.
      
Quantitative and qualitative changes described for small intestine of conventional rats [Bouhours D, Bouhours J-F (1981) Biochem Biophys Res Commun 99:1384-89] were also found in the feces of these germ-free rats.
      
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This paper reported the reseazch work on the establishment of ELISA used for the detection of antibodies against M pulmonis in rats. The reaction with 5μg of lysate antigen per ml and conjugate dilution of 1:16000 resulted in maximum ratio of P/N. OD_(492) value was varied with concentration of antigen and antibodies regularly. The ratio of P/N was higher than 2.1, even though serum was diluted in 1: 1280. Compared with the cultural isolation, ELISA is more sensitive in the detection of natural infection of...

This paper reported the reseazch work on the establishment of ELISA used for the detection of antibodies against M pulmonis in rats. The reaction with 5μg of lysate antigen per ml and conjugate dilution of 1:16000 resulted in maximum ratio of P/N. OD_(492) value was varied with concentration of antigen and antibodies regularly. The ratio of P/N was higher than 2.1, even though serum was diluted in 1: 1280. Compared with the cultural isolation, ELISA is more sensitive in the detection of natural infection of M. pulmonis and the blocking ratio of positive sera was higher then 50%. Evidence above suggested that sensitivity, specificity, and reproducibility of ELISA system are satisfactory. M. pulmonis infcetion was found in 91% of 130 conventional rats tested. The survey also showed the infection of M. arthritidis, Which is mixed infection with M pulmonis. No infection of these two mycoplasma was found in clear rats.

报告了实验大鼠肺支原体感染ELISA检测方法的研究。抗原包被量在0.5μg/ml,酶结合物1:16000稀释时,P/N值最大。波长在492时O.D值随抗原和抗体浓度不同而呈现有规律的变化。阳性血清稀释至1:1280时,P/N值仍在阳性范围之内。与常规的分离培养方法相比较,ELISA的阳性检出率高,且阳性血清的ELISA阻断率均大于50%。重复测定,实验结果的再现性好。以上结果说明所建立的ELISA方法,其敏感性、特异性和重复性均好。在所检测的130只普通级大鼠中,抗体阳性率达91%。另外还发现在实验大鼠中还存在关节支原体的感染,且一般与肺支原体以混合感染的形式存在。在清洁级大鼠中未见这两种支原体的感染。

Based on the identification of antigenicity of PVM, a ELISA Kit, in which antigen was prepared by BHK21 cells inoculated with PVM, was establishment for detecting antibody to PVM in rats The conjugate (specific antibody labled with HRP) was used in ELISA-block test. IFA test was used for further verification of specificity of ELISA Kit. Antibody protein can be detected at a level of 2μg/ml. The results showed the high sensitivity and good reproducibility. This Kit was used in rountine monitoring. The results...

Based on the identification of antigenicity of PVM, a ELISA Kit, in which antigen was prepared by BHK21 cells inoculated with PVM, was establishment for detecting antibody to PVM in rats The conjugate (specific antibody labled with HRP) was used in ELISA-block test. IFA test was used for further verification of specificity of ELISA Kit. Antibody protein can be detected at a level of 2μg/ml. The results showed the high sensitivity and good reproducibility. This Kit was used in rountine monitoring. The results indicated that the infection rate was 38.2% (55/114) in conventional rats and 0% (0/10) in clear rats. Compared with two methods for selection of critical value by using OD value from 154 rats no significant diference was found.

在小鼠肺炎病毒的抗原特性进行鉴定的基础上,将其毒种感染BHK_(21)细胞作为抗原,建立了检测大鼠PVM抗体的酶联免疫吸附试验的试剂盒。经过提纯PVM免疫血清结合辣根过氧化物酶,用于阻断试验、免疫荧光抗体(IFA)试验,考核了本试剂盒的特异性,对该试剂盒的灵敏性及重演性作了验证。并用本试剂盒对154只大鼠的PVM抗体水平进行调查,结果是开放大鼠PVM阳性率为38.2%,清洁大鼠为0。并对154只大鼠标本用光密度OD值定值的两种方法作了探讨。

Using Bacillus piliformis extracted from infected liver homogenate as bacterialantigen, an indirect immunofluorecent assay was established for the detection ofanti-Bacillus piliformis antibody. and proved to be specific to Bacillus piliformis. Bythis method, it was found that the antibody positive rate of SPF rats maintained inisolators was 0 (0/28), while that of conventional rats maintained in open systemwas 40.9% (34/88), indicating different infective rates of rats in different envi-ronments....

Using Bacillus piliformis extracted from infected liver homogenate as bacterialantigen, an indirect immunofluorecent assay was established for the detection ofanti-Bacillus piliformis antibody. and proved to be specific to Bacillus piliformis. Bythis method, it was found that the antibody positive rate of SPF rats maintained inisolators was 0 (0/28), while that of conventional rats maintained in open systemwas 40.9% (34/88), indicating different infective rates of rats in different envi-ronments. The antibody positive rate of conventional mice maintained in the sameopen system was only 3%. As the bacterial antigen used in this method was derivedfrom rat, whether the difference of antibody positive rate between rats and mice reflectsthe different antigenicities of the bacteria derived from different species remains forfurther study.

用大鼠来源的泰泽氏菌芽孢RJ株经口服感染3周龄Wistar系大鼠,获得该菌抗原和抗血清,建立了检测泰泽氏病的免疫荧光法,并证实该法具有特异性。用该法检测不同饲养环境中的大鼠,结果:饲养在隔离器内的SPF级大鼠的抗体阳性率为0(0/28),饲养在开放系统内的普通级大鼠的抗体阳性率为40.9%(36/88),而饲养在同一开放系统内的普通级小鼠的阳性率仅为3%。这种差异是否反映了不同种类动物来源的菌株具有不同的抗原性,尚待进一步研究。

 
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