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   copper-binding 的翻译结果: 查询用时:0.008秒
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copper-binding
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  铜结合
     The conserved region was 595 bp in length, which encoded 195 amino acids. The deduced amino acid sequence contained putative copper-binding domains characteristic of known PPOs, it had a significant homology to known PPO sequence from grape(Vitis vinifera ), tomato(Lycopersicon esculentum ), potato(Solanum tuberosum )and broad bean(Vicia faba ), were 71.3%, 80.8%, 79.8%, and 70.6%, respectively.
     PPO保守区之间的cDNA片段,测序表明该片段含595bp,编码195个氨基酸,推导的氨基酸序列包含了植物PPO的2个保守的铜结合区域,与葡萄(Vitisvinifera )、番茄(Lycopersiconesculentum)、马铃薯(Solanumtuberosum)和蚕豆(Viciafaba )PPO保守区的同源性分别为71.3%、80.8%、79.8%、70.6%。
短句来源
     RESULTSThe deduced amino sequence of mLOXL2 cDNA presented the characteristic domains of lysyl oxidase (LOX) family, the copper-binding site and the catalytic domain.
     [结果 ]mLOXL 2cDNA推断的氨基酸序列呈现赖氨酰氧化酶 (LOX)家族的特征性区域 ,即铜结合部位和催化区域 .
短句来源
     A novel laccase gene (lacE) was cloned from the genomic DNA isolated from Trametes sp. 420, using the degenerate primers based on the conserved copper-binding regions in fungal laccases. Long distance-inverse PCR (LD-IPCR) was used to amplify the flanking sequences of the gene.
     根据真菌漆酶铜结合区保守的氨基酸序列设计简并引物,扩增Trametes sp.420基因组,结合长距离反向PCR(LD-IPCR),成功获得新型漆酶同工酶基因lacE。
短句来源
     Copper-binding proteins can not only protect cells from copper toxicity, but also eliminate superoxide anion toxicity.
     铜结合蛋白不仅可以保护细胞免受铜的毒害,而且可以消除超氧化阴离子的毒性。
短句来源
     In this research, we screened a blue copper-binding protein (BCB) gene and a glutathione-S-transferase (GST) gene from cDNA library of H. villosa. And we cloned three cyclophilin (CyP) genes by PCR according to wheat CyP sequence retrieved from GenBank.
     本研究从抗病簇毛麦cDNA文库中筛选到1个蓝铜结合蛋白基因(BCB)和1个谷胱甘肽硫转移酶基因(GST),并根据数据库中已知的小麦cyclophilin基因(CyP)序列设计特异引物,从簇毛麦cDNA中克隆到3个CyP基因。
短句来源
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  铜离子结合
     [Results] When 1-4 copper-binding domains of ATP7B gene were deleted one by one, the trafficking of ATP7B delayed or diminished.
     【结果】ATP7B基因1-4铜离子结合区逐个缺失后,ATP7B蛋白定向移动减慢至消失;
短句来源
     [Conclusion] The function of six copper-binding domains of ATP7B gene is different. The 1-4 domains induce trafficking of ATP7B within the cell, while the 5-6 copper-binding domains are directly involved in copper transport.
     【结论】ATP7B基因6个铜离子结合区功能不同,其中1-4结合区主要根据铜浓度诱导ATP7B蛋白细胞内定向移动,而5-6结合区则直接参与铜离子细胞外转运。
短句来源
     [Objective] The deletion mutant of copper-binding domains of ATP7B gene was constructed in order to investigate its function in hepatocytes of toxic milk mouse.
     【目的】构建ATP7B基因铜离子结合区缺失突变体,研究其在Tx小鼠肝细胞中的表达及功能。
短句来源
     When 5-6 copper-binding domains were deleted, copper transport stopped.
     5-6铜离子结合区缺失,细胞铜转运功能停滞。
短句来源
     Laccase is one of key enzymes involved in lignin biosynthesis pathway. Homology-based cloning method is available with the highly conserved copper-binding sites for each of the ligand residues and conserved potential N-linked glycosylation sites in laccases.
     漆酶是木质素生物合成途径的关键酶,其高度保守的铜离子结合区以及N末端糖基化位点使利用同源克隆技术成为可能。
短句来源
  “copper-binding”译为未确定词的双语例句
     Impact of synthetucal copper-binding therapy on the biliary copper excretion and hepatolienal ultrasonography of hepatolenticular degeneration
     综合驱铜治疗对肝豆状核变性患者胆汁排铜及肝脾声像图的影响
短句来源
     PURIFICATION AND CHARACTERIZATION OF COPPER-BINDING PROTEIN IN A COPPER-RESISTANT STRAIN OF SACCHAROMYCES CEREVISIAE
     抗铜酿酒酵母铜结合蛋白的纯化及性质研究
短句来源
     This p-type ATPase has three domains. First is metal binding site domain, including six copper-binding sites, which is in the N-termmal segment of HLD gene product.
     此酶主要有三个功能区:①金属离子结合区(metal binding site domain),位于N端,有6个与特定金属结合的氨基酸保守序列,长约30个氨基酸左右。
短句来源
     coli DH10B. The cDNA library contains about 30,0000 recombinant cDNA clones, with average insert size of 1.6kb and is successfully screened with the probes of wheat glutathione reductase gene (Ta-GR), a blue copper-binding protein (BCB) gene, a glutathione-S-transferase (GST) gene and other genes cloned by ourselves.
     其中,利用特异引物,从易位系中分离获得了两个cDNA片段(Ta-RPM,Ta-M1a),它们的核苷酸序列分别与大麦NBS-LRR类抗病蛋白(AAB96982.1)及大麦CC-NBS-LRR抗病蛋白MLA13基因(AF523682.1)序列同源性为64%和87%。
短句来源
     Based on the amino acid sequences deduced from the cDNA sequences, the conserved amino acid residues in copper-binding regions, including ten His and one Cys residues, and several putative N-glycosilyzation sites were found in the peptide chains of these laccase genes.
     基于cDNA推导的氨基酸序列中均含有保守的10个His残基和1个Cys残基,且酶分子中含有多个潜在的N-糖基化位点。
短句来源
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  copper-binding
APP is a major regulator of neuronal copper homeostasis and has a copper-binding domain (CuBD).
      
Two proteins related to neurodegeneration are copper-binding proteins (1) the amyloid precursor protein (APP), a protein related to Alzheimer's disease, and (2) the Prion protein, related to Creutzfeldt-Jakob disease.
      
Isolation and characterization of copper-binding sites of human ceruloplasmin
      
The potential copper-binding sites, from Trp-196 to Tyr-245, and from Asn-366 to Phe-421, are highly homologous to the corresponding sites in other invertebrates.
      
γ-Globulin molecule has several copper-binding sites differing by binding constants and filled one-by-one as the content of bound metal increased.
      
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Hepatolenticular degeneration (HLD) is an autosomal recessively inherited disease caused by copper metabolic dysfunction. Its primary genetic defect is still not known. A previous study showed that the average copper content of HLD cells was approximately threefold that of normal cells. Cultured fibroblasts were used as an in vitro model to investigate primary molecular defects. We examined the distribution of protein-bound intracellular copper in proteins of HLD cells. Cell lysates were fractionated by gel...

Hepatolenticular degeneration (HLD) is an autosomal recessively inherited disease caused by copper metabolic dysfunction. Its primary genetic defect is still not known. A previous study showed that the average copper content of HLD cells was approximately threefold that of normal cells. Cultured fibroblasts were used as an in vitro model to investigate primary molecular defects. We examined the distribution of protein-bound intracellular copper in proteins of HLD cells. Cell lysates were fractionated by gel fitration. Copper concentration of columnar fractions were determined by anodic stripping voltammetry. Results revealed that the character of copper binding was altered in HLD cells.A decreased ratio of copper to proteins was observed in cytoplasmic proteins having a molecular weight≥30 000. There was more copper specifically bound to lower molecular weight compounds in HLD cells, these results also may serve as a method for early unequivocal diagnosis of this di sorder.

在已经建立的肝豆状核变性(HLD)细胞模型基础上,用凝胶层析法和阳极溶出伏安法,研究铜在皮肤成纤维细胞不同蛋白组分中分布。结果:HLD患者细胞中第一洗脱蛋白峰的铜/蛋白质比值大大低于对照,患者过量累积铜主要与小分子蛋白质结合。此法可用于临床对该病的早期诊断。

Ssveral experiments have been done to demonstrate molybdate sulphur and their compound-trithiomolybdate could affect the metabolism of copper bound proteins in liver cytosol, especially copper on the metallothioncin. A possibility is that molybdate strip copper from metallothionein in the cytosol of liver of cattle. Copper stripped either moved out of cells as a small molecullar compound in vivo, or combined to a large molecullar proteins in cytosol in vitro. Meanwhile, metallothionein may capture copper from...

Ssveral experiments have been done to demonstrate molybdate sulphur and their compound-trithiomolybdate could affect the metabolism of copper bound proteins in liver cytosol, especially copper on the metallothioncin. A possibility is that molybdate strip copper from metallothionein in the cytosol of liver of cattle. Copper stripped either moved out of cells as a small molecullar compound in vivo, or combined to a large molecullar proteins in cytosol in vitro. Meanwhile, metallothionein may capture copper from other source, for example, a large molecullar copper bound proteins. The peak of metallothionein appcars when thiomolybdate excreted from cells. Dithiothretol can stablize the combination of copper to metallothionein. There is no obvious effect on the combination of copper to the proteins when samples were stored in -20℃ for five weeks.

以8头公犊为材料,通过体内和体外试验证明硫、钼及其化合物三硫钼酸盐被吸收或直接注入血液进入肝脏后,对肝内铜代谢的干扰作用主要是促使铜与咪噻宁蛋白质分离。分离后的铜成为小分子化合物离开肝细胞,而在体外试验中,还有部分铜转移到分子量较大的蛋白质上。一旦硫钼酸盐从肝内排泄后,体内铜很快与咪噻宁重新结合。缓冲液中二硫苏糖醇可使铜与咪噻宁结合保持恒定,样品在冰冻情况下保存5周,对咪噻宁与铜结合作用无明显影响。

In the paper, the action of metal ions on the human serum albumin had been investigated by fluorescence quenching method. The results showed that Cu~(2+), Mn~(2+), Co~(2+), Cr~(3+),Eu~(3+),Ni~(2+) may bind to human serum albumin and that the fluorescence of human serum albumin may be quenched by nonradiative energy transfer. In terms of Forster type dipoledipole nonradiative energy transfer the distance between the Trp-214 and the bound Cu~(2+) in the first copper-binding site was calculated and found...

In the paper, the action of metal ions on the human serum albumin had been investigated by fluorescence quenching method. The results showed that Cu~(2+), Mn~(2+), Co~(2+), Cr~(3+),Eu~(3+),Ni~(2+) may bind to human serum albumin and that the fluorescence of human serum albumin may be quenched by nonradiative energy transfer. In terms of Forster type dipoledipole nonradiative energy transfer the distance between the Trp-214 and the bound Cu~(2+) in the first copper-binding site was calculated and found to be about 1.20nm.

通过金属离子对人血清白蛋白内源荧光的猝灭,探讨了金属离子Cu~(2+),Mn~(2+),Ni~(2+),Co~(2+),Cr~(3+)等与人血清白蛋白的结合;基于Forster无辐射能量转移机理,得出了人血清白蛋白第一类Cu~(2+)结合部位与214位色氨酸残基间的距离。

 
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