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demonstrated clearly
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  清楚显示
     Results Of 42 cases 35(83%) duodenal papillae were demonstrated clearly by MRI.
     结果42例患者中,MRI清楚显示35例十二指肠乳头,占全部病例的83%。
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     Conclusion:Brain and spinal cord MS plaques can be demonstrated clearly on MRI,MRI had important significance in the diagnosis of MS.
     结论:MRI可清楚显示MS病灶,对MS的诊断具有重要意义。
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     All the lesions were demonstrated clearly by contrast enhancement examination while 13 cases showed unclear on T1WI-FLAIR sequence, 5 cases on FRFSE-T2WI sequence and 2 cases on T2WI-FLAIR sequence.
     T1WIFLAIR显示病灶欠清或不清13例,FRFSET2WI、T2WIFLAIR显示欠清或不清楚的分别为5例、2例,增强扫描均清楚显示病灶。
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     The results indicated that the thymus is located in the supra anterior mediastinum and could be demonstrated clearly on CT scan.
     结果显示,胸腺位于前上纵隔,CT扫描可清楚显示胸腺。
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     Results:The images of CPR displayed clearly the entire course of ureter and demonstrated clearly a calculus actually within the ureter, including the stone size, shape, location and the obstruction degree of ureter in all 52 cases.
     结果:52例CPR图像均能显示输尿管全程,并能清楚显示输尿管内结石大小、部位、形态以及结石上方输尿管梗阻扩张程度。
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  “demonstrated clearly”译为未确定词的双语例句
     RII study showed that xenografted tumor was demonstrated clearly on the 3rd and 5th day after injection of 125I labeled L4B4.The T/NT was greater than 4 on the sib day.
     荷瘤裸鼠RII研究显示:注入125I-L4B4后第3天及第5天可清晰显示肿瘤影像,第5天的T/NT>4。
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     All the lesions were demonstrated clearly by MSCT and the sensitivity was 100%. 44 cases of 53 were correctly staged with TNM and the accuracy was 83.0%. The accuracy of T,N and M was 94.3%(50/53),86.8%(46/53),and 98.1%(52/53),respectively.
     MSCT检出了全部大肠癌,敏感度为100%,对大肠癌TNM分期的准确度为83.0%(44/53),其中T分期准确度为94.3%(50/53),N分期的准确度为86.8%(46/53),M分期的准确度为98.1%(52/53)。
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     The wall of the carotid artery,the blood flow,the size and the content of the plaque could be demonstrated clearly by using T1WI,T2WI,PDWI and 3D-TOF.
     T1WI、T2WI、PDWI和3D-TOF的序列组合能准确显示血管壁、血流、斑块大小、形态及斑块内成分。
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     The results showed that(1) All lesions larger than 1.0 cm in diameter and their relations with surrounding structures could be demonstrated clearly by MR.
     结果表明:(1)MR可清晰显示大于1.0cm的病灶及其与周围结构的关系;
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     By means of theories of liberalism, cultural internationalism, idealism and constructivism, the Cultural Diplomacy's origin, roles, organizers and its connections with national power and international order's construction, would be demonstrated clearly.
     运用自由主义、文化国际主义、理想主义和建构主义等国际关系理论,论述文化外交的思想缘起、文化外交中的教育改造功能、文化与权力和文化与国际秩序的关系、以及文化外交行为体多元化的特征。
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     It was demonstrated that D-T.
     用分离出的D-T.f S~(2-)氧化缺陷型菌株进行浸出,发现它比具有这种氧化能力的正常菌株的浸出效果差得多。
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     However the mechanism is not clearly demonstrated.
     但其作用机制尚不明确。
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     The research purpose and meaning is clearly demonstrated.
     阐明了研究目的和意义。
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     It has been demonstrated with FPGA.
     该方案已通过FPGA验证.
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  demonstrated clearly
Densitometric analysis demonstrated clearly measurable signals for both mRNAs.
      
However, such barriers are demonstrated clearly in only one of the two pairs of sympatric species sampled more intensively.
      
Amplitude histograms for eIPSC demonstrated clearly detectable equally spaced peaks.
      
It has been demonstrated clearly that the tail peptide β-CN-(f193-209) is important in maintaining the hydrophobic core of β-CN but the residual association observed argues for a minor role for other sites as well.
      
Parameters including the arthritis index, chemiluminescence and anti-collagen antibody titers were used for the assessment of disease activity: The disease courses demonstrated clearly an inhibitory effect of thalidomide.
      
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The present investigation was undertaken in 1965-1966 to study the oxidative phosphorylation and the fine structure of the flight muscle mitochondria in the housefly Musca domestica vicina Macq. Comparisons were made between the normal (= susceptible) and DDT-resistant strains before and after DDT treatment in order to analyse the relationship between structure and function of the mitochondria. The results may be summarized as follows:1. The mitochondrial suspensions prepared from the flight muscles oxidized...

The present investigation was undertaken in 1965-1966 to study the oxidative phosphorylation and the fine structure of the flight muscle mitochondria in the housefly Musca domestica vicina Macq. Comparisons were made between the normal (= susceptible) and DDT-resistant strains before and after DDT treatment in order to analyse the relationship between structure and function of the mitochondria. The results may be summarized as follows:1. The mitochondrial suspensions prepared from the flight muscles oxidized rapidly a-glycerophosphate and succinate, but slowly or did not oxidize most of the other citric acid cycle intermediates such as pyruvate, a-ketoglutarate, malate and fumarate as well as β-hydroxybutyrate and glutamate. The same results could be obtained when the muscle homogenate or the mitochondria plus supernatant were tested. This result may serve as the evidence in favour of the view that the isolated housefly mitochondria are not readily permeable to these intermediate compounds of citric acid cycle.2. In the cases where a-glycerophosphate was used as the substrate, a high rate of respiration (Qo2 = 413.3) and a high yield of oxidative phosphorylation (P/0 -2.00) may be obtained in the following incubation conditions: the dilute mitochondrial suspension (about 2 mg of protein of mitochondria in 2.0 ml reaction mixture), the short incubation time (about 10 minutes), and the addition of bovine plasma albumin.3. The rates of respiration and oxidative phosphrylation of the mitochondria were significantly inhibited by DDT in vitro and in vivo, but the degree of inhibition was different in normal and DDT-resistant houseflies. In the mitochondria from the normal strain the inhibition of respiration and that of phosphorylation caused by DDT (0.1 mM) in vitro were 40% and 60.9% respectively, whereas in the DDT-resistant strain the corresponding values were 23.4% and 42.7%. AVhen bovine plasma albumin was added into the incubation medium, the inhibition of respiration and that of phosphorylation of the normal housefly mitochondria were 71.1% and 94.8%, whereas in the DDT-resistant houseflies the values were 59.7% and 87.1% respectively. When the houseflies were treated with DDT (0.5%) suspension in vivo, the inhibitions of respiration and phosphorylation in the normal housefly mitochondria were 21% and 33%, whereas in DDT-resistant houseflies the values were 11.5% and 26.9% respectively. After the addition of bovine plasma albumin into the reaction medium the inhibitions of respiration and phosphorylation in the normal housefly mitochondria were 7.0% and 6.1% respectively, whereas in DDT-resistant houseflies no inhibition was observed. These results indicated that the DDT-resistant houseflies in some degree exhibited at the level of mitochondrial function more resistance to the action of DDT than the normal strain.4. An electron microscopic examination on the fine structure of the flight muscle mitochondria from the two strains of the housefly also confirmed that at the level of the cellular organelles the DDT-resistant houseflies were more resistant to the action of DDT. After the insects were treated with DDT in vivo, the mitochondra from the normal strain showed marked lysis, but in the DDT-resistant strain only some mitochondria were slightly damaged. The result demonstrated clearly that in the housefly the changes in structure and function of the mitochondria were correlative during the development of insecticide resistance.

本文报道有关家蝇胸肌线粒体氧化磷酸化作用和亚显微结构的实验和观察结果。试验证明,家蝇胸肌线粒体在离体条件下对α-甘油磷酸有很高的氧化和磷酸化活力,而对三羧酸循环各底物及其他氧化底物的氧化和磷酸化速率则极为微弱。胸肌匀浆以及线粒体与上清液的重组合试验结果和单独用分离的线粒体试验结果基本相同,从而有利于说明造成上述现象的原因可能不是由于线粒体在制备过程中受到损伤,而是家蝇胸肌线粒体膜对这些底物存在着特殊的透性屏障。在以α-甘油磷酸为氧化底物时,线粒体在下述保温条件下可获得很高的氧化速率和P/O比值:线粒体浓度较稀(2毫升反应液中含1—2毫克线粒体蛋白);保温时间较短(10—20分钟之内)以及外加一定量的牛血浆清蛋白(0.2%)。DDT对线粒体的氧化和磷酸化反应均表现强烈的抑制作用,但在正常家蝇和抗性家蝇中则表现出一定的差别。在离体试验的条件下,DDT(0.1mM)对正常家蝇线粒体的氧化抑制40%,对磷酸化抑制60.9%,而抗性家蝇则分别为23.4%和42.7%;在反应系统中外加牛血浆清蛋白时,DDT对正常家蝇线粒体的氧化抑制71.1%,磷酸化抑制94.8%,而抗性家蝇则分别为59.7%和87.1%。在DDT(5%...

本文报道有关家蝇胸肌线粒体氧化磷酸化作用和亚显微结构的实验和观察结果。试验证明,家蝇胸肌线粒体在离体条件下对α-甘油磷酸有很高的氧化和磷酸化活力,而对三羧酸循环各底物及其他氧化底物的氧化和磷酸化速率则极为微弱。胸肌匀浆以及线粒体与上清液的重组合试验结果和单独用分离的线粒体试验结果基本相同,从而有利于说明造成上述现象的原因可能不是由于线粒体在制备过程中受到损伤,而是家蝇胸肌线粒体膜对这些底物存在着特殊的透性屏障。在以α-甘油磷酸为氧化底物时,线粒体在下述保温条件下可获得很高的氧化速率和P/O比值:线粒体浓度较稀(2毫升反应液中含1—2毫克线粒体蛋白);保温时间较短(10—20分钟之内)以及外加一定量的牛血浆清蛋白(0.2%)。DDT对线粒体的氧化和磷酸化反应均表现强烈的抑制作用,但在正常家蝇和抗性家蝇中则表现出一定的差别。在离体试验的条件下,DDT(0.1mM)对正常家蝇线粒体的氧化抑制40%,对磷酸化抑制60.9%,而抗性家蝇则分别为23.4%和42.7%;在反应系统中外加牛血浆清蛋白时,DDT对正常家蝇线粒体的氧化抑制71.1%,磷酸化抑制94.8%,而抗性家蝇则分别为59.7%和87.1%。在DDT(5%)处理整体家蝇时,正常家蝇线粒体的氧化被抑制21%,磷酸化被抑制33%,而抗性家蝇则分别为11.5

Cytochrome b-563, a major b type cytochrome in a facultative anaerobes-Klebsiela rhinoscleromatis was isolated and extracted from the acetone powder of this bacterium with phosphate buffer by the repeated "freezing and thawing" method. It had been purified more than 800 folds from the cell-free crude extract by ammonium sulfate fractionation, cation exchange on Zerolit 226 and chromatography on diethyl aminoethyl cellulose. The purified pigment was showed homogeneous on agarose gel electrophoresis.The oxidized...

Cytochrome b-563, a major b type cytochrome in a facultative anaerobes-Klebsiela rhinoscleromatis was isolated and extracted from the acetone powder of this bacterium with phosphate buffer by the repeated "freezing and thawing" method. It had been purified more than 800 folds from the cell-free crude extract by ammonium sulfate fractionation, cation exchange on Zerolit 226 and chromatography on diethyl aminoethyl cellulose. The purified pigment was showed homogeneous on agarose gel electrophoresis.The oxidized absorption spectra of cytochrome b-563 had peaks at 420, 535 nm and the reduced form had major peaks at 430, 533, 563nm.The pyridine hemochromogen absorption spectra had peaks at 419, 527, 557nm. The above spectral characteristics of cytochrome b-563 and its pyridine haemochromogen demonstrated clearly that pigment belonged to the b-group of cytochrome.

兼性厌气菌鼻硬结克雷白氏杆菌(Klebsiella rhinoscleromatis)具有不完整呼吸链,主要含细胞色素b-563,还可能含细胞色素d。可以用冻化法直接从鼻硬结克雷白氏杆菌丙酮干粉中分离及抽提细胞色素b-563。应用硫酸铵分部沉淀、Zerolit 226阳离子树脂柱层析及DEAE纤维素柱层析可将细胞色素b-563从粗抽出液中纯化800倍以上。纯化的细胞色素b-563在琼脂糖凝胶电泳中均一。氧化型细胞色素b-563的吸收峰为420、535毫微米,还原型为430、533、563毫微米。吡啶血色素原的吸收峰为419、527、557毫微米。根据光谱特性可以说明上述细胞色素属于b型细胞色素。

At present it is well known that the mitochondria are formed by a close intereac-tion of two distinct genetic systems: the nucleo-cytoplasmic system and the mitochon-drial system. However, it is not quite clear how mitochondrial membranes in the cell are assembled. The present investigation provides some evidences indicating that during the process of mitochondria biogenesis in armyworm species the outer mitochondrial membrane is formed first, followed by the inner mitoehondrial membrane and crestae system;...

At present it is well known that the mitochondria are formed by a close intereac-tion of two distinct genetic systems: the nucleo-cytoplasmic system and the mitochon-drial system. However, it is not quite clear how mitochondrial membranes in the cell are assembled. The present investigation provides some evidences indicating that during the process of mitochondria biogenesis in armyworm species the outer mitochondrial membrane is formed first, followed by the inner mitoehondrial membrane and crestae system; but in locust species the mitochondria biogenesis process begins with the formation of inner mitoehondrial membrane vesicles followed by the outer mitochondria] membrane. The experimental results obtained from these two insects are summarized as follows:Ⅰ. Electronmicroscopic examinations (Plates 1:1-4, 11:5-6) indicated that the mitochondria of armyworm are formed from non-membraneous precusors (macromole-cule particles). In the early stage of differentiation and development of flight muscle of this insect (8th day of pupal stage) the outer mitoehondrial membranes are just being assembled (Plate 1:1). From this period on the mitochondria develop and grow rapidly. Finally, the mitochondria of adult moth become a complicated systems with well-developed inner membranes (Plate 11:5-6). The biochemical determinations (Q02, P: O and ATPase activity) of these mitoehondrial preparations have demonstrated clearly that during the process of mitochondria genesis the change of structure corelates with a change of function. Observations on the respiratory rate of intact pupa and the cytochrome oxidase activity of pupal tissue homogenate have indicated that mitochcti-dria assembly become more active beginning from the 8th day of pupal stage. Another series of experiments has shown that in this period no oligomycin-sensitive ATPase-Fi activity can be detected. This suggests that at the early stage of mitochondria formation ATPase-F1 are not yet synthesized or they are not assembled on the inner mitochondrial membranes.Ⅱ. During the 4th instar of larval development of locust differentiation of flight muscle just begins while the mitochondria are being formed. It seems that the inner mitoehondrial membrane vesicles are formed first and then the outer mitoehondrial membrane. In accordance with these there is also a gradual increase in Qo2, P: O as well as in the oligomycin-sensitive ATPase activity from 4th instar larva to adult locust. It was also found that in the 4th instar locust larva mitochondria had no head-like F1 on the inner mitoehondrial membrane and very low oligomycin-sensitive ATPase activity was detected.From these experimental results the following points are made: the mitochondria of the developing insect flight muscle during metamorphosis are formed gradually from non-membraneous precursors, different enzymes are assembled on the inner mitoehondrial membranes at different stages of development, and the peptides synthesized by mitoehondrial genetic system seem to have more importance in the early stage of assembly of mitochondria membranes.

线粒体在细胞中的发生目前有各种观点的争论,其理论意义涉及到真核细胞的起源和进化、染色体和线粒体两个遗传体系之间的相互关系以及生物膜合成和组装机理等。我们对处于分化中的昆虫胸肌线粒体的观察结果是:(1)对粘虫变态期的呼吸和细胞色素氧化酶活力测定表明蛹期第8天的组织形成阶段是胸肌细胞分化和其线粒体发生的开始。电镜观察表明,线粒体形成分两个阶段:由颗粒结构(可能是酶蛋白与脂的复合体)装配成膜片和膜泡;由膜泡分化出内嵴,进而发育为线粒体。(2)QO_2值,P/O比和ATP酶活力的出现与膜结构的分化发育相平行。α-甘油磷酸氧化酶系统比谷氨酸氧化酶系统装配早;电子传递酶系比磷酸化酶系装配早。(3)蝗虫胸肌分化过程的电镜观察证明;先形成内膜小泡(直径约0.1微米左右),后形成外膜,组成简单线粒体;后者进一步分化发育为成熟线粒体。(4)QO_2值,P/O比和ATP酶活力与膜结构分化发育相平行。ATP酶的出现与能量转涣功能呈平行关系。膜形成早期和“幼稚”线粒体阶段,ATP酶尚未装配。(5)综合上述结果:线粒体膜由非膜结构逐步组装形成,线粒体内膜的各酶系组装次序不同步,线粒体DNA控制合成的膜蛋白在膜结构形成中似乎起核心和骨架作...

线粒体在细胞中的发生目前有各种观点的争论,其理论意义涉及到真核细胞的起源和进化、染色体和线粒体两个遗传体系之间的相互关系以及生物膜合成和组装机理等。我们对处于分化中的昆虫胸肌线粒体的观察结果是:(1)对粘虫变态期的呼吸和细胞色素氧化酶活力测定表明蛹期第8天的组织形成阶段是胸肌细胞分化和其线粒体发生的开始。电镜观察表明,线粒体形成分两个阶段:由颗粒结构(可能是酶蛋白与脂的复合体)装配成膜片和膜泡;由膜泡分化出内嵴,进而发育为线粒体。(2)QO_2值,P/O比和ATP酶活力的出现与膜结构的分化发育相平行。α-甘油磷酸氧化酶系统比谷氨酸氧化酶系统装配早;电子传递酶系比磷酸化酶系装配早。(3)蝗虫胸肌分化过程的电镜观察证明;先形成内膜小泡(直径约0.1微米左右),后形成外膜,组成简单线粒体;后者进一步分化发育为成熟线粒体。(4)QO_2值,P/O比和ATP酶活力与膜结构分化发育相平行。ATP酶的出现与能量转涣功能呈平行关系。膜形成早期和“幼稚”线粒体阶段,ATP酶尚未装配。(5)综合上述结果:线粒体膜由非膜结构逐步组装形成,线粒体内膜的各酶系组装次序不同步,线粒体DNA控制合成的膜蛋白在膜结构形成中似乎起核心和骨架作用;线粒体总组装过程在不同细胞中表现为多种途径和方式。

 
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