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during cultivation
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  培养期
     The cytotoxicity grade of the material was determined according to six-grade. All cells were proliferated during cultivation. The cytotoxicity gradation of each group was at 0~1 grade.
     培养期各组细胞大量增殖,毒性级为0~1级,各实验组与阴性对照组比较无显著性差异(P>0 05);
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  培养过程
     Batch culture,fed batch culture and perfusion culture were used,the maximuim viable cell density reached 2.0×10 6,2.7×10 6 and 9.6×10 6? mL -1 ,respectively. The concentration of nutrients (glucose and amino acid) and metabolic by products (ammonia and lactate) were analyzed during cultivation period.
     3种方法最大活细胞密度分别达到了 2 .0× 10 6,2 .7× 10 6和 9.6× 10 6mL-1.对培养过程中葡萄糖和氨基酸及代谢产物氨和乳酸的动态变化进行了检测和分析 .
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     During cultivation in vitro the NPC and Hela cell can be seen. The synthesis of β_2-MG is following propagation of these cells. So we suppose mat the serum β_2-MG Increase of NPC patients may be origented from the cancar cell's synthesis and their release, but this is non specific.
     鼻咽癌及Hela细胞在体外培养过程中,亦见随着细胞的增殖可以不断合成β_2-MG,故可以推测鼻咽癌病人血清中β_2-MG的升高,可能来源于癌细胞的合成和释放,但非特异性。
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     Discussion on Innovation Education During Cultivation of Postgraduate
     研究生培养过程中的创新教育探析
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     The physiological and biochemical characteristics of degradating lignocelluloses of Polyporus frondosus was studied during cultivation of the fungus in oax-sawdust and wheat-bran medium.
     本文对贝叶多孔菌(Polyporus frondosus)在柞树木屑——麦麸基物上生长期间基物的降解特性和培养物中胞外纤维素酶、半纤维素酶和淀粉酶在培养过程中的活性变化规律及其它一些生物化学性质进行了研究。
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     In the combination technology,when 1% of nicotinic acid was kept in cultivation medium by supplement of nicotinic acid at intervals during cultivation of Pseudomonas putida NA-1,the growing culture could consistently hydroxylate nicotinic acid without degradation of its product which was accumulated linearly in the cultivation medium with progress of cultivation;
     该工艺在恶臭假单胞菌NA-1培养过程中持续补充烟酸以维持1%(W/V)浓度,使烟酸被生长细胞转化为羟基化烟酸并在发酵液中线性积累,而不被进一步降解;
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  “during cultivation”译为未确定词的双语例句
     Effect of NaH_2PO_4 and NAA on Test-Tude Plant of Orient Cherry During Cultivation
     NaH_2PO_4及NAA对樱花试管苗壮苗培养的影响
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     The recombinant plasmid was transferred into thecompetent BL21 (DE3) cells and approximately a 28.6 kDa fusion protein was induced by theaddition of isopropyl-β-D -thiogalactoside (IPTG) (1.0 mM) during cultivation.
     将重组质粒pET-28a(+)-p25转化到大肠杆菌BL21(DE3),加入终浓度为1.0 mM的异丙基-β-D-硫代半乳糖苷(isopropyl-β-D -thiogalactoside, IPTG)进行诱导,诱导后表达出分子量大约为28.6 kDa的P25融合蛋白。
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     When Mn2+ concentration was 0.03 g/L,the proportion of D-glutamic acid remained constant throughout cultivation,approximately 75%,this was different from B. subtilis F-2-01,the stereochemical composition of γ-PGA produced by the microorganism changed during cultivation.
     当Mn2+浓度为0.03g/L时,发酵过程中生成的γ-PGA中D-谷氨酸比例不变,维持在75%左右.
短句来源
     The result show that ZL 9601 strain were rod bacteria in morphology(0.8 ̄1.0×1.0 ̄2.5μm), oxygen needed during cultivation. The optimum temperature was 30℃ and optimum pH was 7.4 ̄7.2. the favorite carbon source was glucose.
     结果表明,该菌为短杆菌(0.8~1.0×1.0~2.5μm),需氧,发酵最适温度30℃,最适pH7.0~7.2,最适碳源为葡萄糖,最适氮源为硫酸铵;
短句来源
     During cultivation in the fermentor a highest pullulan yield of 60% was obtained. The pullulan molecular weight ranged from 61.7×10 4~5.5×10 4 Daltons.
     发酵罐中培养得到最高多糖产率 6 0g/L ,多糖分子量大小在 6 1.7× 10 4 ~ 5 .5× 10 4 D之间
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  during cultivation
Utilization of cotton oil soap stock as the only source of carbon during cultivation of the fungus was studied.
      
The most effective utilization was observed during cultivation of the fungus in the medium containing soap stock.
      
The abundance of some strains considerably decreased during cultivation.
      
Factors Contributing to Roquefortine Yield Variability during Cultivation of Penicillium roquefortii
      
The addition of menadione into the medium during cultivation ofNeurospora crassa in the dark activated its constitutive superoxide dismutase.
      
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Electron microscopy is of advan-tage for diagnosis and identification of the histogenetic classification of lung tumors.Using this techniques,an ultrastructural observation on the cha-racteristics and its dynamic changes during cultivation from 1~(st) to 5~(th) day of human lung adenocarcinoma cell line (SPC-A-1) was made. The cultivated cells of 3~(rd)-4~(th) day were more complicated in structure than that of 1~(st)-2~(nd) and 5~(th) day.They showed higher activity of secreting func-tion and more increased...

Electron microscopy is of advan-tage for diagnosis and identification of the histogenetic classification of lung tumors.Using this techniques,an ultrastructural observation on the cha-racteristics and its dynamic changes during cultivation from 1~(st) to 5~(th) day of human lung adenocarcinoma cell line (SPC-A-1) was made. The cultivated cells of 3~(rd)-4~(th) day were more complicated in structure than that of 1~(st)-2~(nd) and 5~(th) day.They showed higher activity of secreting func-tion and more increased in cytoplas-mic constituents.At this stage,the nuclei of cells appeared more hetero-morphic.The majority of nuclei were irregular in contour.Only a small ??amount of short rough endoplasmic reticulum dispersed in cytoplasm,some were extended with very few riboso-mes forming flat empty spaces.Mi-tochondria were swollen,showing dis-solution of cristae and vacuolation.The amount of mucous granules in-creased gradually with the increase of days of subculture.They often fused with each other and some were connected with the intracellular spa-ces.Some mucus accumulated peculiar-ly in the degenerated mitochondria.In peripheral region,many microvilli and short cytoplasmic processes existed,a number of pinocytotic vesicles,coated vesicles and many intracellular cannules could be seen,extending deeply into cytoplasm.All these indicated the en-hanced activity of cellular metabo-lism.However,the cells of 1~(st)-2~(nd) and 5~(th) day were seen simplified in structures.Mucous granules were very few at these stages. It should be pointed out,the in-creased production of mucous gra-nules closely related to cellular activity and their unusual accumulation in degenerated mitochondria and rough endoplasmic reticulum appeared in quite different pan from normal mucin-secreting cells. Besides,mucous granules and va-rious forms and sizes of lamellar bo-dies similar to that of Type II alveolar epithelial cells indicated that this cell line was probably originated from human bronchiolar epithelial cells with mucous metaplasia during carcino-genesis.

本文对体外培养的人肺腺癌细胞(SP-C-A-l)进行了传代周期1—5天的超微结构观察。从第49、61、68和84代细胞的观察结果表明,传代培养5天过程中,细胞的超微结构发生明显变化。主要表现在胞质内粘液颗粒的数量随着细胞进入对数生长期(3—4天)逐渐增多,并有粘液在异常的部位(变性的线粒体)积聚;与此同时,线粒体的数量相应增加,内部结构出现明显改变。当细胞生长活动处于相对稳定阶段(1—2天和5天),粘液颗粒和变性的线粒体明显减少。细胞的高尔基复合体不发达以及正常形态的粗面内质网少的情况,在传代5天内变化不大,提示该系腺癌细胞粘液颗粒的形成与正常的粘液腺细胞不尽相同。结合细胞内有肺泡Ⅱ型上皮细胞特有的板层体的存在,文中进一步讨论了SP-C-A-l细胞系可能来源于人的细支气管上皮细胞在细胞癌变过程中发生粘液化生,而使细胞具有合成和分泌粘液的功能。

Our stndies showed that an immunopotentiator, S-O_2-1 vaccine, Was able to induoe interferon in mouse spleen cells in Vitro. Spleen cells of C_(57)BL/6 mice inoculated with S-O_2-1 Vaccine, after 48hrs of cultivation elaborated high level of interferon. suchh activity could first be detected 4 days after inoculation, reached maximal titer (7.47±0.31 log_2 units/ml) at 7 days and subsequently declined. On the other hand, if S-O_2-1 vaccine was added during cultivation, the maximal level was delayed until...

Our stndies showed that an immunopotentiator, S-O_2-1 vaccine, Was able to induoe interferon in mouse spleen cells in Vitro. Spleen cells of C_(57)BL/6 mice inoculated with S-O_2-1 Vaccine, after 48hrs of cultivation elaborated high level of interferon. suchh activity could first be detected 4 days after inoculation, reached maximal titer (7.47±0.31 log_2 units/ml) at 7 days and subsequently declined. On the other hand, if S-O_2-1 vaccine was added during cultivation, the maximal level was delayed until 10 days, but with significantly higher titer. In unprimed conditions, when 5×10~(6) spleen cells, of normal C_(57)BL/6 mice were cultured in the presence of lx10~7 cells/ml of S-O_2-1 vaccine, interferon activity was detected as early as 12hrs after and reached a maximal level (8.23±0.30 log_2 units/ml) at 48hr. Further study revealed that the interferon induced by S-O_2-1 vaccine possessed similar physicochemical properties as the type-Ⅱ interferon. In addition, S-O_2-1 vaccine also enhanced the production of interferon by mouse spleen cells stimulated with Con A and the amount of interferon produced in the presence of both agents was greater than the sum total of interferon stimulated by each agent acting alone.

本文表明S-O_2-1菌苗具有较强的诱导小鼠脾细胞产生干扰素的能力。将事先用菌苗免疫的C57BL/6小鼠脾细胞体外培养时,即使不加菌苗也能产生较高滴度的干扰素活性,该活性在免疫后4天制备的脾细胞培养中开始出现,免疫后7天则达高峰(7.47±0.31 log_2U/ml),以后逐渐下降。若培养时添加菌苗,干扰素高峰出现稍晚些,于免疫后10天达高峰,但滴度明显上升(9.10±0.26 log_2U/ml)。另外,C57BL/6正常小鼠的脾细胞(5×10~6细胞/ml)添加菌苗(0.1亿菌/ml)体外培养12小时后可开始测出干扰活性,培养48小时后活性达高峰(8.23±0.30log_2U/ml)。该干扰素具有小鼠Ⅱ-型干扰素相同的特性。我们在实验中还发现,将S-O_2-1菌苗与刀豆素A(Con A)合用诱生的干扰素滴度明显高于两者单独使用时诱生滴度的累加值。

The genus Pythium was estabilished by Pringsheim in 1858 and included about one hundred of readily recognized species with wide distributions and hosts ranges up to now from the world.A great number of Pythium isolates have been securved from various kinds of soil and infected plant tissues throughout this country.The present paper recorded 45 species of Pythium in China.And ecological study and geographical distribution were conducted in the meantime of studying taxonomy. Pythium species are ecologically versatile...

The genus Pythium was estabilished by Pringsheim in 1858 and included about one hundred of readily recognized species with wide distributions and hosts ranges up to now from the world.A great number of Pythium isolates have been securved from various kinds of soil and infected plant tissues throughout this country.The present paper recorded 45 species of Pythium in China.And ecological study and geographical distribution were conducted in the meantime of studying taxonomy. Pythium species are ecologically versatile and physiologically unique fungi.They are ubi- quitous in soil and aquatic environments,worldwide in distribution,and have wide host ranges. They are among the most important and destructive plant pahogens because they cause destroyings of seeds;damping-off of seedlings;rots of roots;and decays of fruits and vegetables during cultivation,storage,transit or at the market. The result of this study revealed that the number of Pythium in the investigated soil varied with differen sources: soils from vegetable gardens are quite common and rather abundant;soils from crop field waterside,zoo,garden,park as well as graveyard rank next;soils from arid sand forest and salty swamp are generally poor;soils from grassland,meadow,roadside,moun- tainous region and barren hillock are very rare.In general,they are more abundant in moist, fertile and cultivated lands. In vegetable graden soil the vertical distribution of Pythium species in Beijing district was largely confined to the top 5—15 cm of soil where the soil was enriched with organic material in association with higher moisture.The seasonal variation of Pythium was quite distinct: spring is the most favourable for their activity,autumn ranks next,summer is the least in number. The distribution of the Pythium species in Chinese soil was variable: P.pulchrum and P. aphanidermatum were the most widely distributed species in this country;P.spinosum,P.sinense and P.carolinianum were in next place;on other contrary,10 species including P.acrogenum,P. coloratum,P.connatum etc.appeared only once a time. As for the geographical distribution of the genus Pythium in 30 provinces,cities and auto- nomous regions of China,22 species were recorded in Beijing (Peking) district,13 species were reported from provinces of Hebei,Guangdong and Yunnan simultaneously,Taiwan province has 12 species,and in provinces of Gansu and Xinjiang only one species was wound.No record of Pthiurn has been reported in autonomous regions of Ningxia and Xizing (Tibet),and Oinghai province.

腐霉属 Pythium 真菌全世界已报道约100种。本文记载我国的腐霉45种,以及它们的生态及地理分布。腐霉喜栖息于富含有机质的潮湿土壤里,以菜园土中为最多,其次是大田土、水畔土和陵园土,干旱的沙质土、森林土和含盐碱的沼泽土一般均较少,最贫乏的是草丘土,如草原、牧场、山坡、荒丘及道旁等土壤。在我国的土壤中,以绚丽腐霉P·pulchrum 和瓜果腐霉 P·aphanidermatum 最为常见,其次是刺腐霉 P.spinosum、中国腐霉 P.sinense和卡地腐霉 p.carolinianum,而顶生腐霉 P.acrogenum、色孢腐霉 P.coloratum、壁合腐霉P.connatum 等10个种却较罕见,仅出现过一次。腐霉在土壤中的垂直分布以5—10cm 深处为最多。腐霉在土壤中的活动有着明显的季节性变化:春季最多,秋季次之,夏季最少。在我国的30个省市自治区中,北京发现的腐霉最多,已报道22种;河北、广东、云南省次之,各有13种;台湾省已记录12个种;山西省、甘肃省和新疆维吾尔自治区均仅发现一个种;而青海省、宁夏回族自治区和西藏自治区迄今尚未见有腐霉报道。

 
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