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exonuclease activity
相关语句
  外切核酸酶活性
     Taq pol, MMLV- RT and human pol 茁 are polymerases lack of 3′-5 ′ exonuclease activity.
     Taq聚合酶(Taqpol)、失去核酸酶结构域的鼠白血病逆转录酶(MMLVRT-)和人源的聚合酶#(pol$)缺少3′→5′外切核酸酶活性.
短句来源
     They also show that the ATP-dependent dsDNA exonuclease activity of the RecBC enzyme has nothing to do with the recA gene in chromosome replication.
     实验结果还说明,RecBC酶的依赖于ATP的双链DNA外切核酸酶活性和recA基因的作用无关。
短句来源
     [WT5”HZ]Conclusion:[WT5”BZ]It is a economical and effective way to amplify longer DNA fragment after some enzymes which has 3’→5’ exonuclease activity and proofreading function were added to the normal PCR system. [WT5”HZ]
     结论 :在一般的 PCR体系中添加适量的具有 3’→ 5’外切核酸酶活性和校正功能的Tli DNA聚合酶扩增稍长 DNA片段是经济可行的
短句来源
  “exonuclease activity”译为未确定词的双语例句
     P53Δ37 Protein Exhibits 3′ 5′ Exonuclease Activity
     P53Δ37蛋白3′-5′核酸外切酶活性的初步研究
短句来源
     SPR Biosensor Study of The Molecular Recognition Between The DNA Polymerase Without 3′→5′ Exonuclease Activity With DNA
     SPR生物传感器研究缺乏3′→5′核酸外切酶活性的DNA聚合酶对DNA的分子识别功能
短句来源
     Pfu DNA polymerase, having 3'-5' exonuclease activity, is one of the DNA polymerases that have the highest fidelity.
     Pfu DNA酶具有3’-5’外切酶活性,是保真度最高的DNA聚合酶之一。
短句来源
     Exo has 5'→3' exonuclease activity, that can generate 3'-extruding ends of dsDNA.
     Exo具有5’端到3’端的外切酶活性,可以产生3’突出末端;
短句来源
     In the present study, we intended to know whether mutated P53 protein—P53Δ37 has this 3′ 5′exonuclease activity or not.
     本研究对一种缺陷型 P5 3蛋白 P5 3Δ 37是否具有这种核酸外切酶活性进行初步研究。
短句来源
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  相似匹配句对
     The activity of E.
     淀粉酶活性在50℃,pH 6.0条件下最高.
短句来源
     Academic activity
     学术活动
短句来源
     Effect of 3′ exonuclease activity of polymerase on extension of phosphorothioate-modified primers
     聚合酶3′外切活性对3′硫化修饰引物聚合反应的影响
短句来源
     P53Δ37 Protein Exhibits 3′ 5′ Exonuclease Activity
     P53Δ37蛋白3′-5′核酸外切酶活性的初步研究
短句来源
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  exonuclease activity
We were unable to detect any DNA polymerase, DNA ligase, or 5' or 3' exonuclease activity associated with this purified material.
      
A closer examination of the neuronal exonuclease activity revealed that bases are excised from the 3' end in a sequential and nonspecific manner, although initial excision of a mismatched base was slightly faster.
      
The Presence of 3'-5' Exonuclease Activity in Rat Brain Neurons and Its Role in Template-Driven Extension of 3'-Mismatched Prime
      
Here we show that Trm2p purified as a fusion protein displayed 5' → 3' exonuclease activity on double-strand (ds) DNA, and endonuclease activity on single-strand (ss) DNA, properties characteristic of previously isolated endo-exonucleases.
      
NM23-H1 is a metastasis suppressor protein that exhibits 3'-5' exonuclease activity in vitro.
      
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Hind Ⅲ and Hind Ⅱ have been purified from Haemophilus influenzae Rd strain.Some problems in the purification procedure were investigated.Endonucleolyticdigestion of DNA from plasmid pBR322 and phage λcI857S7 has been examined andthe DNA fragments resolved by agarose electrophoresis.The digestion profilesproduced are unique and characteristic for both DNA's and the endonucleases.The ligation of Hind Ⅲ generated fragments indicates the existence of cohesiveends of the Hind Ⅲ products.It also shows that the preparation...

Hind Ⅲ and Hind Ⅱ have been purified from Haemophilus influenzae Rd strain.Some problems in the purification procedure were investigated.Endonucleolyticdigestion of DNA from plasmid pBR322 and phage λcI857S7 has been examined andthe DNA fragments resolved by agarose electrophoresis.The digestion profilesproduced are unique and characteristic for both DNA's and the endonucleases.The ligation of Hind Ⅲ generated fragments indicates the existence of cohesiveends of the Hind Ⅲ products.It also shows that the preparation of Hind Ⅲis free of significant exonuclease activity.The molecular weight of Hind Ⅲ wasestimated to be 135,000 as determined by polyacrylamide gel electrophoresis,Seph-adex G150 gel filtration,and sucrose density gradient centrifugation.Electro-phoresis in the presence of SDS revealed two polypeptides,MW 70,000 and 62,000,which is consistent with the presence of nuclease and methylase componentsin this enzyme.

从流感嗜血杆菌(Haemophilus influenzae)Rd 株中纯化了限制性内切核酸酶HindⅢ及部份纯化了Hind Ⅱ;探讨了纯化过程中的某些问题;检查了酶对底物双链DNA 的特异性内切作用,从琼脂糖平板凝胶电泳中观察到的经消化后产生的DNA 断片表明:酶对各种底物具有专一性作用;通过DNA 断片的重新连接证明:经Hind Ⅲ消化后生成的断片具有粘性末端,也说明纯化的Hind Ⅲ中不合明显的外切酶活力;用聚丙烯酰胺-十二烷基硫酸钠平板凝胶电泳,葡聚糖凝胶G150分子筛层析及蔗糖密度梯度离心法测定了Hind Ⅲ的分子量并对其亚单位进行了讨论.

We have reported in our previous paper that replication of E. colt chromosome initiated by the integrated F' placmid depends, on the recA gene. Here we report on work dealing with the role played by recA, recB, recC, lexA alleles in chromosome replication. Our results show that the recA gene takes part in chromosome replication through homologous recombination rather than SOS pathway and that Chi hot spot is not concerned with. They also show that the ATP-dependent dsDNA exonuclease activity of the RecBC...

We have reported in our previous paper that replication of E. colt chromosome initiated by the integrated F' placmid depends, on the recA gene. Here we report on work dealing with the role played by recA, recB, recC, lexA alleles in chromosome replication. Our results show that the recA gene takes part in chromosome replication through homologous recombination rather than SOS pathway and that Chi hot spot is not concerned with. They also show that the ATP-dependent dsDNA exonuclease activity of the RecBC enzyme has nothing to do with the recA gene in chromosome replication.

我们在前文中报道由整合的F'质粒所发动的大肠杆菌染色体的复制依赖于recA基因。本文报道有关recA、recB、recC以及lexA等在染色体复制中的作用,实验结果说明,recA基因通过同源重组途径而不是通过SOS途径参与复制,而且recA基因和Chi热点无关。实验结果还说明,RecBC酶的依赖于ATP的双链DNA外切核酸酶活性和recA基因的作用无关。

Objective To explore a method for eliminating the shadow bands in PCR amplification of trinucleotide repeats.Methods CTG trinucleotide repeat sequence in the 3′ untranslated region of the human myotonin protein kinase gene was used as templates DNA in PCR amplification, and the effects of Taq DNA polymerase alone and its mixture with Pwo DNA polymerase on occurrence of the shadow bands' were compared. The PCR products containing (CTG) 5, both from Taq DNA polymerase and from Taq+Pwo polymerase amplification,...

Objective To explore a method for eliminating the shadow bands in PCR amplification of trinucleotide repeats.Methods CTG trinucleotide repeat sequence in the 3′ untranslated region of the human myotonin protein kinase gene was used as templates DNA in PCR amplification, and the effects of Taq DNA polymerase alone and its mixture with Pwo DNA polymerase on occurrence of the shadow bands' were compared. The PCR products containing (CTG) 5, both from Taq DNA polymerase and from Taq+Pwo polymerase amplification, were cloned into pBluecript KS and sequenced on ALF express TM DNA sequencer respectively. Results The PCR of DNA sequence containing CTG repeats frequently produced a main band (usually darker) and a shadow band (lighter) that differed from the main band by 3 base pairs when Taq DNA polymerase was used alone. However, when the mixture of Taq DNA polymerase and Pwo DNA polymerase was used, the shadow bands usually disappeared. In the 4 clones containing PCR products amplified by Taq DNA polymerase, one clone contained only 4 CTG repeats. However, this kind of decrease of repeat copy was not observed in the 5 clones containing PCR products amplified by using mixture of Taq+Pwo DNA polymerase. Conclusion The results of this experiment demonstrate that shadow bands occur during the PCR. Pwo DNA polymerase in known to possess not only DNA polymerase activity, but also 3′-5′ exonuclease activity, or proofreading ability. The mixture of Taq DNA polymerase and Pwo DNA polymerase has higher processivity than Taq DNA polymerase itself. This may explain the effect on eliminating or reducing the occurrence of shadow bands in polymerase chain reaction.

目的为了消除PCR扩增三核苷酸重复序列时产生的影子带。方法以人类强直性肌营养不良致病基因-肌张力蛋白激酶基因(myotoninproteinkinasegene,MT-PK)3′端非翻译区中的CTG重复序列为例,主要比较了PCR反应中单独使用TaqDNA聚合酶和使用Taq+Pwo混合的DNA聚合酶对影子带产生的影响。结果实验表明PCR反应中单独使用TaqDNA聚合酶时,其PCR产物经PAGE电泳,银染显色后常有影子带形成,而向TaqDNA聚合酶中加入具有3′-5′外切酶活性的PwoDNA聚合酶则可完全消除影子带。结论本实验结果表明影子带产生于PCR过程本身,而比TaqDNA聚合酶具有更高加工性的组合酶则有可能消除或减弱影子带。

 
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