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hepatitis delta virus ribozyme
相关语句
  hdv核酶
     The Screening of HBV Targeting Hepatitis Delta Virus Ribozyme Vectors
     HBV靶向性HDV核酶载体的筛选
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  “hepatitis delta virus ribozyme”译为未确定词的双语例句
     The plasmid was constructed containing full-length antigenome cDNA of CC-47 that was under control of T7 promoter and joined with hepatitis delta virus ribozyme sequence.
     克隆麻疹病毒CC-47株蛋白N、P、L编码区质粒并置于T7启动子控制下,用4个质粒共转染哺乳动物细胞,在表达T7RNA聚合酶的重组痘苗病毒VTF7-3的作用下进行病毒拯救。
短句来源
  相似匹配句对
     The Molecular Biology of Hepatitis delta virus
     丁型肝炎病毒的分子生物学研究进展
短句来源
     STUDIES ON CELLULAR IMMUNITY OF DELTA HEPATITIS
     HDV感染者细胞免疫状态的研究
短句来源
     CHRONIC TYPE B HEPATITIS WITH DELTA FACTOR INFECTION
     慢性乙型肝炎患者的HDV感染
短句来源
     PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES TO HEPATITIS DELTA VIRUS
     抗丁型肝炎病毒单克隆抗体的制备及其生物学特性
短句来源
     Hepatitis B Virus
     乙型肝炎病毒
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  hepatitis delta virus ribozyme
Properties of Antigenomic Hepatitis Delta Virus Ribozyme That Consists of Three RNA Oligomer Strands
      
The correct 3h end is ensured by the use of the hepatitis delta virus ribozyme sequence.
      
Requirement for canonical base pairing in the short pseudoknot structure of genomic hepatitis delta virus ribozyme.
      


To establish a reverse genetic system of measles virus from its full-length antigenomic cDNA, six overlapped fragments covering full length cDNA of measles virus CC-47 strain were amplified by RT-PCR.Three plasmids encoding nucleocapsid protein,phosphoprotein and large protein of measles virus were constructed and were under the control of T7 RNA polymerase promoter.The plasmid was constructed containing full-length antigenome cDNA of CC-47 that was under control of T7 promoter and joined with hepatitis delta...

To establish a reverse genetic system of measles virus from its full-length antigenomic cDNA, six overlapped fragments covering full length cDNA of measles virus CC-47 strain were amplified by RT-PCR.Three plasmids encoding nucleocapsid protein,phosphoprotein and large protein of measles virus were constructed and were under the control of T7 RNA polymerase promoter.The plasmid was constructed containing full-length antigenome cDNA of CC-47 that was under control of T7 promoter and joined with hepatitis delta virus ribozyme sequence.B95a cell was cotransfected with the four plasmids after infection with a helper virus VTF7-3.Rescued CC-47 virus was identified by immunoflurescence, RT-PCR and sequencing.Measles virus was rescued from the system,after three passages of the transfected stock on B95a cell measles virus could still be detected by IFA and RT-PCR.

为发展新型疫苗和改造目前使用的麻疹病毒疫苗,以麻疹病毒疫苗株为模板,构建了具有感染性的麻疹病毒cDNA克隆。用RT-PCR分6段扩增出麻疹病毒全长基因,通过酶切、拼接构建麻疹病毒疫苗株CC-47的全长正链cDNA序列,并精确地置于T7启动子控制下与丁型肝炎病毒核酶序列之前。克隆麻疹病毒CC-47株蛋白N、P、L编码区质粒并置于T7启动子控制下,用4个质粒共转染哺乳动物细胞,在表达T7RNA聚合酶的重组痘苗病毒VTF7-3的作用下进行病毒拯救。经免疫荧光、PCR等方法检测证实,获得了具有感染性的麻疹病毒。所拯救的病毒在哺乳动物细胞连续传3代后,仍能检出病毒抗原和核酸。

 
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