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heterologous plants
相关语句
  异源植物
     PCR and Southern analysis proved that Pti5-VPl6 gene has already integrated into the genome of the tobacco,and the expression vector has a right open read frame that can express in heterologous plants.
     经卡那霉素筛选,获得了27株卡那霉素抗性植株,经PCR扩增和Southern杂交分析,证明抗性植株中整合了Pti5-VP16基因,表明所构建的含Pti5-VP16基因的植物表达载体pBI121UCH1具有正确的阅读框,并能在异源植物中表达。
短句来源
     Transposons have been used extensively for insertional mutagenesis of endogenous or heterologous plants for gene isolation and gene cloning.
     转座子已经被广泛用于内源或异源植物插入突变的研究 ,用于基因的分离和克隆。
短句来源
  “heterologous plants”译为未确定词的双语例句
     This review focuses on the transposons widely used and how it was successfully used in heterologous plants for insertional mutagenesis and as a tool of reverse genetics in the functional genomics study.
     本文综述了一些广泛应用的转座子以及这些转座子如何成功应用于异源植物的插入突变 ,作为反向遗传学的工具在研究功能基因组学中应用
短句来源
  相似匹配句对
     Advances in studies of the production of heterologous proteins in plants
     利用植物生产异源蛋白的研究进展
短句来源
     Plants are particularly attractive as bioreactors for heterologous protein production.
     植物作为生产异源蛋白的生物反应器,近年来颇受关注。
短句来源
     Cortile and Plants
     中庭植物
短句来源
     Lipoxygenase in Plants
     植物的脂肪氧化酶
短句来源
     Studies on the Heterologous Expression of MTG
     轮枝链霉菌转谷氨酰胺酶异源表达研究
短句来源
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  heterologous plants
Furthermore, the expression of S-VSPs in heterologous plants led to a significant increase in total soluble lysine, suggesting that these proteins may also permit better assimilation of lysine by humans and monogastric animals.
      
Thus, the G9 promoter can be used to drive gene expression in homologous as well as heterologous plants in a tissue-specific manner.
      
To investigate if the altered ERS1 gene can affect the tissue senescence in heterologous plants, we introduced it into coriander by Agrobacterium-mediated transformation.
      
Adapted versions of this regeneration technique have a high potential for providing germinal excision-like events in heterologous plants species which show low transposon activity.
      
Engineering novel phytoalexins in heterologous plants provides a unique strategy to enhance plant innate defense systems.
      


Leaf segments of tobacco were infected by Agrobacterium tumefaciens EHA105 with plasmid pBI121UCHl ,and then 27 kanamycin resistant plants were obtained. PCR and Southern analysis proved that Pti5-VPl6 gene has already integrated into the genome of the tobacco,and the expression vector has a right open read frame that can express in heterologous plants.

以烟草叶片为外植体,通过根癌农杆菌叶盘转化法,将番茄Pti5-VP16基因导入烟草中。经卡那霉素筛选,获得了27株卡那霉素抗性植株,经PCR扩增和Southern杂交分析,证明抗性植株中整合了Pti5-VP16基因,表明所构建的含Pti5-VP16基因的植物表达载体pBI121UCH1具有正确的阅读框,并能在异源植物中表达。

Transposons have been used extensively for insertional mutagenesis of endogenous or heterologous plants for gene isolation and gene cloning. With the rapid development of genome research in several plants, more gene sequences with unknown function should be identified by the reverse genetics. Large scale trasposon mutagenes has become the important approach for the functional genomics. This review focuses on the transposons widely used and how it was successfully used in heterologous plants...

Transposons have been used extensively for insertional mutagenesis of endogenous or heterologous plants for gene isolation and gene cloning. With the rapid development of genome research in several plants, more gene sequences with unknown function should be identified by the reverse genetics. Large scale trasposon mutagenes has become the important approach for the functional genomics. This review focuses on the transposons widely used and how it was successfully used in heterologous plants for insertional mutagenesis and as a tool of reverse genetics in the functional genomics study.

转座子已经被广泛用于内源或异源植物插入突变的研究 ,用于基因的分离和克隆。随着不同植物基因组研究进程加快 ,需要通过反向遗传学来研究已知序列基因的功能 ,大规模的转座子突变已成为功能基因组学研究的一种很重要手段。本文综述了一些广泛应用的转座子以及这些转座子如何成功应用于异源植物的插入突变 ,作为反向遗传学的工具在研究功能基因组学中应用

Because of the concern about escape of antibiotic or herbicide resistant transgenes from transgenic crops, selectable marker genes from plant origin would be an alternative choice for plant transformation. In this study, a feedback insensitive anthranilate synthase gene ( ASA2 ) cloned from a tobacco cell line was tested for Agrobacterium mediated transformation of axis tissue of soybean mature embryo, with a tryptophan analogue 5 methyltryptophan (5 MT) as the selective agent. Southern...

Because of the concern about escape of antibiotic or herbicide resistant transgenes from transgenic crops, selectable marker genes from plant origin would be an alternative choice for plant transformation. In this study, a feedback insensitive anthranilate synthase gene ( ASA2 ) cloned from a tobacco cell line was tested for Agrobacterium mediated transformation of axis tissue of soybean mature embryo, with a tryptophan analogue 5 methyltryptophan (5 MT) as the selective agent. Southern blot analysis of the T 0 transgenic lines confirmed the integration of the ASA2 gene into the soybean genome. Northern blot analysis showed the ASA2 gene was also expressed in the leave tissue, and the free tryptophan content in the leaf tissue of transgenic soybean was about 59% to 123% more than that in the wild type. PCR analysis of the T 1 progeny showed that the transgene was inherited in a Mendelian fashion. All these results indicate that this feedback insensitive ASA2 gene can be used as a selectable marker gene for plant transformation. This work also demonstrated that the ASA2 gene coding for the α subunits from one plant (tobacco) can interact with the β subunits of a heterologous plant (soybean) to form an active anthranilate synthase enzyme. The use of this feedback insensitive gene as a novel selectable marker for plant transformation is also discussed.

目前广泛采用的抗菌素或抗除草剂基因作为植物转化筛选标记基因可能带来转基因逃逸 ,因此寻找能够用于植物转化的来源于植物本身的筛选基因是解决这一问题的方法之一。通过从烟草中克隆的邻氨基苯甲酸合成酶基因 (ASA2 )作为筛选标记基因 ,并采用氨基酸的类似物 5 甲基色氨酸为筛选剂 ,进行了农杆菌介导的大豆成熟胚尖转化研究。Southern杂交结果表明ASA2基因成功整合到大豆基因组 ,Northern杂交也显示该基因在转化大豆叶片中表达。HPLC检测转化大豆叶片游离色氨酸的含量比野生型要高 5 9%~ 12 3%。PCR检测转化子 1代结果显示转化基因通过孟德尔规律稳定遗传。这些结果表明反馈抑制不敏感ASA2基因可以作为筛选标记基因用于大豆遗传转化。同时也证实来源于一种植物 (烟草 )编码的邻氨基苯甲酸α 亚基能够与另一种植物 (大豆 )编码该酶的 β 亚基结合形成具有完整活性的邻氨基苯甲酸合成酶。对ASA2基因作为一种新的植物转化筛选标记基因的优缺点进行了讨论

 
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