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horseradish peroxidase complex
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  偶联辣根过氧化酶复合物
     analysed by means of SDS polyacrylamide gel electrophoresis (SDS PAGE) and followed by Western blotting. The membranes were incubate with biotinylated wheat germ agglutinin (WGA/Bio) and avidin biotin horseradish peroxidase complex (ABC kit) respectively, then developed with 4 chloro 1 naphthol.
     血小板样品经 SDS-聚丙烯酰胺凝胶电泳 (SDS- PAGE)后 ,再转移至硝酸纤维素膜上(Western blotting) ,用生物素化的麦胚凝集素 (WGA/ Bio)偶联辣根过氧化酶复合物试剂盒 (ABC kit)分步孵育 ,再以 4-氯 - 1-萘酚显影。
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  “horseradish peroxidase complex”译为未确定词的双语例句
     To promot sensitivity of immunodiagnosis to Schistosomiasis japonicum, a new bioenlargement,home-made biotin-avidin system(BAS)was used in ELISA with avidin-biotinylated horseradish peroxidase complex (ABC) , and was compared with routine ELISA.
     应用新的生物放大系统BAS作ABC-ELISA,与常规ELISA比较检测可提高试验的敏感性。
短句来源
  相似匹配句对
     Horseradish
     辣根
短句来源
     Determination of Horseradish Peroxidase by Spectrophotometry
     3,3′,5,5′-四甲基联苯胺-H_2O_2-HRP分光光度法测定HRP的研究
短句来源
     Progress of the Mimetic Enzyme of Horseradish Peroxidase
     辣根过氧化物酶模拟酶研究进展
短句来源
     ON "FAULTED COMPLEX"
     浅议“断裂杂岩”
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     Polyelectrolyte Complex
     聚电解质复合物
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  horseradish peroxidase complex
A surfactant-horseradish peroxidase complex was prepared with a nonionic surfactant by utilizing water-in-oil emulsions.
      
Surfactant-horseradish peroxidase complex catalytically active in anhydrous benzene
      
Implants or injections of the wheat germ agglutinin-horseradish peroxidase complex have revealed that all five subdivisions project to different parts of the cerebellar cortex and nuclei.
      
The cerebellar projection from the Edinger-Westphal nucleus was studied in the cat by means of retrograde transport of the wheat germ agglutinin-horseradish peroxidase complex.
      
By means of retrograde transport of the wheat germ agglutinin-horseradish peroxidase complex, afferent fibres to the lateral reticular nucleus from the oculomotor and accessory oculomotor nuclei were demonstrated in the cat.
      
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An avidin-biotinyl-horseradish peroxidase complex (ABC for brevity) p obe, whcih was prepared by using a highly reactive and specific polyclone treated with biotin to aim at the target of antigns from both worms and eggs of schistosoma dotted on the nitrocellulose membrane, was developed. The method was based on the principle of biotin-avidin system to amplify the reactivity multiplicatively. The test was performed by using the granules of polyacrylamide gel to concentrate the serum sample and to dissociate...

An avidin-biotinyl-horseradish peroxidase complex (ABC for brevity) p obe, whcih was prepared by using a highly reactive and specific polyclone treated with biotin to aim at the target of antigns from both worms and eggs of schistosoma dotted on the nitrocellulose membrane, was developed. The method was based on the principle of biotin-avidin system to amplify the reactivity multiplicatively. The test was performed by using the granules of polyacrylamide gel to concentrate the serum sample and to dissociate the circulated immunocompiex.

本文建立了一种以吸附在硝酸纤维素纸上的日本血吸虫循环抗原为靶子,用生物素化的抗虫源性和虫卵源性的高效特异的多克隆抗体为探针检测血吸虫循坏抗原的方法。该法利用生物素一亲和素的多级放大作用,并用聚丙烯酰胺凝胶颗粒浓缩血清及解离复合物等方法来提高敏感性。显示这种探针阳性率高、假阳性率低用于诊断具有较好的敏感性和特异性。该探针与DNA探针比较具有简便、快速、不需特殊仪器等优点,可望成为确诊病人和考核疗效的工具。

Avidin-biotin-horseradish peroxidase complex-enzyme linked immunosorbent assay (ABC-ELISA), standard enzyme linked immunosorbent assay (ELISA), indirect hemagglutination test (IHA), counterimmunoelectrophoresis (CIEP) and Caso-ni's introdermal test(ID)were performed and evaluated for their sensitivities and specificities in 101 patients, 40 patients with miscellaneous non-hydatid diseases andei normal subjects. The sensitivities were: ABC-ELISA. 94.06%, to ELISA, 81.19%, IHA, 87.13%, CIEP. 71.29%, ID:...

Avidin-biotin-horseradish peroxidase complex-enzyme linked immunosorbent assay (ABC-ELISA), standard enzyme linked immunosorbent assay (ELISA), indirect hemagglutination test (IHA), counterimmunoelectrophoresis (CIEP) and Caso-ni's introdermal test(ID)were performed and evaluated for their sensitivities and specificities in 101 patients, 40 patients with miscellaneous non-hydatid diseases andei normal subjects. The sensitivities were: ABC-ELISA. 94.06%, to ELISA, 81.19%, IHA, 87.13%, CIEP. 71.29%, ID: 87.50% respectively. The ID gave much higher false positive rate (37.50%) . The specific ities of the other four serological tests! were from 94.06% to 100%. The results show that the positive rate of anti-E. G antibody in patients with pulmonary cyst was lower than that in those with hepatic cyst. In patients with intact pulmonary cyst full of clear hydatid,fluid the positive rate was the lowerest.But in patients with recurrent or ruptured cyst the positive rate was very high and no difference of antibody response was found between hepatic cyst and pulmonary cyst in these patients. It is suggested that the main factor which may affect the antibody response of hy datidosis patients be the "screen effect of an intact cyst wall.

用ABC法等五种免疫学方法可检测了101例包虫病人,40例其它病人及61例正常人抗E.g抗体。各法敏感性为,ABC为94.06%,ELISA为81.19%,IHA为87.13%,CIEP为71.29%,ID为87.50%。ID假阳性率较高(37.50%),而其余四种方法均获较高特异性(94.06~100%)。分析结果表明,初发且包虫囊完整的肺包虫病人抗体反应性最低,复发或囊破裂的病人抗体反应强且无因包虫囊肿寄生部位不同造成的抗体反应的差异。故认为:影响包虫病人特异性抗体反应的主要因素可能是包虫囊壁的屏障作用。

To promot sensitivity of immunodiagnosis to Schistosomiasis japonicum, a new bioenlargement,home-made biotin-avidin system(BAS)was used in ELISA with avidin-biotinylated horseradish peroxidase complex (ABC) , and was compared with routine ELISA.Using SEA, the positive rate was 100% in ABC-ELISA, and 95.6% in routine ELISA among 68 cases with less than 5 miracidia in stool hatching examination. When AWA was used, the ratio of positive reaction OD to negative OD (P/N) in ABC-ELISA was 1.42 times than that...

To promot sensitivity of immunodiagnosis to Schistosomiasis japonicum, a new bioenlargement,home-made biotin-avidin system(BAS)was used in ELISA with avidin-biotinylated horseradish peroxidase complex (ABC) , and was compared with routine ELISA.Using SEA, the positive rate was 100% in ABC-ELISA, and 95.6% in routine ELISA among 68 cases with less than 5 miracidia in stool hatching examination. When AWA was used, the ratio of positive reaction OD to negative OD (P/N) in ABC-ELISA was 1.42 times than that in routine ELISA. The results of testing rabbit schistosomiasis showed that sensitivity was increased by using ABC-ELISA significantly ( P<0.01 ).It will be seen that BAS system is useful in increasing the sensitivity for detecting the specific antibody of Schistosoma japonicum in human and experimental animals.

应用新的生物放大系统BAS作ABC-ELISA,与常规ELISA比较检测可提高试验的敏感性。采用可溶性虫卵抗原对粪孵毛蚴在5只以下30克粪的68例进行检测,ABC法的检出率可由ELISA的95.6%提高到100%;用成虫抗原时,ABC法的阳性消光值和阴性消光值之比(P/N)为常规ELISA的1.42倍。ABC法用于检测实验家兔血吸虫病可显著提高敏感性(P<0.01),提示BAS系统在人及动物特异性抗体的检测中都是有用的增敏试剂。

 
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