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in the cells    
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  组细胞
    Results Typical morphological features of DC were found in the cells of both the two groups by inverse microscopy and electron microscopy. High expression levels of CD1α, HLA-DR, CD80, and CD40 were also found in the cells of the both groups.
    结果 两组细胞在倒置显微镜和扫描电镜下均显示典型的树突状细胞形态 ,流式细胞仪分析均高表达CD1α、HLA DR、CD80和CD4 0分子 ;
短句来源
    The results showed that nearly no GPI-PLD activity was detected in control groups whose PLAP activity was mainly distributed in the cell pellets, whereas high levels of GPI-PLD activity were discovered in the cells transfected with pcDNA3.1(+)/GPI-PLD.
    结果显示 ,对照组细胞几乎检测不到GPI PLD活性 ,其表达的PLAP主要位于细胞膜上 ;
短句来源
    Compared with the cells in the control group, the changes in the number of necrotic cells, concentrations of glucose and lactate, and LDH activity in the cells of the hypoxic group were significantly different after 10 h of hypoxia (P<0.05). However, the flat and polygonal morphology of the astrocytes almost remained unchanged after 8 h of hypoxia in spite of significant changes in the concentrations of glucose and lactate and LDH activity.
    与对照组相比,缺氧10h组死亡细胞数、葡萄糖和乳酸盐的浓度及乳酸脱氢酶(LDH)活性的变化均有明显差异(P<0.05),且与缺氧时间呈正相关,其中缺氧组细胞培养上清液中葡萄糖浓度比对照组明显降低,而乳酸盐浓度和LDH活性则明显升高,而缺氧8h组虽然葡萄糖和乳酸盐的浓度及LDH活性的变化明显,但星形胶质细胞保存着多突触的形态。
短句来源
  在细胞
    To understand the stress induced growth, the study of the transference and distribution of the external force in the cells level inside of the tissue is a key problem.
    要弄清“应力—生长关系”机理,首先要了解宏观的外力如何传递到细胞中去的和外力的刺激是如何在细胞间分布和传递,即要研究宏观的组织受力与细胞层次受力的关系。
短句来源
    They perform different functions in the cells. RNA polymerase Ⅰ synthesizes the three largest rRNAs, 5.8S , 18S and 28S rRNA. RNA polymerase Ⅱ mainly produces mRNA encoding proteins, and RNA polymerase Ⅲ makes 5S rRNA and tRNAs , as well as a few small nuclear RNAs.
    在细胞中它们的功能各不相同:RNA聚合酶Ⅰ负责催化合成5.8S、18S和28S rRNA,RNA聚合酶Ⅱ主要负责mRNA的转录合成,而RNA聚合酶Ⅲ则负责催化5S rRNA、tRNA、多数的Sn RNA以及某些病毒基因(如VA基因)等的转录。
短句来源
    With the ie-1 promotor of Bombyx mori nucleopolyhydrovirus,the DNA vector of RNAi was constructed,which can transcribe dsRNA in the cells to form hairpins and complementary to the region from +19~+446 of ie-1 gene of BmNPV.Experiments demonstrated that the DNA vector inhibited the replication of BmNPV both in vivo and in vitro.
    利用家蚕核型多角体病毒ie-1基因启动子,构建了在细胞内能转录形成与ie-1基因+19~+446区域互补同源的发夹状dsRNA的RNA i DNA载体。 研究结果表明,该载体DNA在体外、体内均能较好地抑制家蚕核型多角体病毒在细胞中的复制。
短句来源
    The active targets of the aneugens in the cells were notonly chromosome and DNA but also all kinds of mitosisapparatuses (such as centriole, kinetochore, microtubule ofspindle etc ),centromere and cell membrane.
    非整倍体诱发剂在细胞中的作用靶标通常不仅是染色体和DNA,也可包括各种有丝分裂器(如中心粒、着丝点、纺锤体微管),着丝粒和细胞膜。
短句来源
    Plasmid pHTX42 constructed from shuttle vector pHT304 was transformed the B. thuringiensis acrystalliferous strain XBU001 with electroporation to obtain the recombinant HTX42. The recombinant protein was found with a molecular mass of 130 kD on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Scanning analysis indicated that the expressed protein accounted up to 79.28% of total cellular proteins and accumulated in the cells mounted up to 64.13% of cellular dry weight.
    利用穿梭载体pHT304构建表达质粒pHTX42,电转化Bt无晶体突变株XBU001,获得重组菌株HTX42,经SDS-PAGE分析,cry1Ac基因得到强表达,蛋白质定量分析显示目的蛋白量占总蛋白量的79.28%,且其在细胞中累积达细胞干重的64.13%,这种产量比文献报道的25%左右增高了一倍以上。
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  在细胞内
    When growing anaerobically in the light using sulfide as electron donor, strain HD_1, HD_2 and HD_4 deposited sulfur granules in the cells.
    菌株HD_1、HD_2和HD_4在厌氧光照条件下利用硫化物,且在细胞内贮存硫粒。
短句来源
    (2) The metabolic behaviour of oxHDL2 in the cells is similar to HDL2. Both of them do not take a lysosomal pathway.
    (2)ox-HDL2在细胞内代谢方式与HDL2相似,均没有经历溶酶体分解途径。
短句来源
    With the ie-1 promotor of Bombyx mori nucleopolyhydrovirus,the DNA vector of RNAi was constructed,which can transcribe dsRNA in the cells to form hairpins and complementary to the region from +19~+446 of ie-1 gene of BmNPV.Experiments demonstrated that the DNA vector inhibited the replication of BmNPV both in vivo and in vitro.
    利用家蚕核型多角体病毒ie-1基因启动子,构建了在细胞内能转录形成与ie-1基因+19~+446区域互补同源的发夹状dsRNA的RNA i DNA载体。 研究结果表明,该载体DNA在体外、体内均能较好地抑制家蚕核型多角体病毒在细胞中的复制。
短句来源
    Apoproteins and CE components dissociate from endocytic lipoprotein in the cells.
    在细胞内载脂蛋白与胆固醇酯(CE)组分经历一个解离过程。
短句来源
    Their interaction may suggest a new mechanisim to localize AP2α in the cells and to regulate the transcriptional activity of AP2α. 
    它们的相互作用可能与AP 2α在细胞内分布及其调控基因转录的方式有关.
短句来源
  后细胞
    The adipose drops began to cumulate in the cells,and to the most quantity until the 21 st day.
    无血清分化培养基培养4d后细胞形态逐渐变圆,并出现球性脂滴,脂滴的数量逐渐增多至分化培养的第21天到达顶峰。
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  in the cells
Enzyme activity was revealed in the cells grown on medium with elemental sulfur or in the presence of various sulfide minerals and concentrates of sulfide ores.
      
The mutants varied in Cr(VI) resistance, the degree of chromium accumulation in the cells (from 0.1 to 11.6 mg/g dry cells), and the degree of Cr(VI) reduction (from 50% to complete disappearance of bichromate from the culture liquid).
      
Treatment with ambiol enhanced the differentiation of the internal membrane system of plastids in the cells of original and transgenic plants, especially the tubular membrane systems.
      
The concentration of eight kinds of catechins in solution decreased by 29.6-47.6%, respectively; some catechins were absorbed and accumulated by yeast cells, but the amount in the cells was very low during the fermentation process.
      
Specific attention is given to regulation of enzymes of antioxidative defense, operating in the cells of strict anaerobes under the conditions of oxidative stress caused by oxygen, superoxide anion, or hydrogen peroxide.
      
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1. The albino mice were used for this study. The Golgi apparatus in the cells of the epididymis and the vas deferens of normal, starved, and refed animals were studied.2. The amount of the Golgi elements can be maintained by feeding, not only by the sex hormone.3. The Golgi apparatus may be regarded as a product of food assimilation, not a definite cell organ.4. It is suggested that the Golgi apparatus has similar organ with that of mitochondria and secretion granules, but with different quantity...

1. The albino mice were used for this study. The Golgi apparatus in the cells of the epididymis and the vas deferens of normal, starved, and refed animals were studied.2. The amount of the Golgi elements can be maintained by feeding, not only by the sex hormone.3. The Golgi apparatus may be regarded as a product of food assimilation, not a definite cell organ.4. It is suggested that the Golgi apparatus has similar organ with that of mitochondria and secretion granules, but with different quantity of lipoids.

(1)本研究以白鼠为材料,就常态白鼠绝食及再餵之白鼠观察其副睾丸及输精管上皮细胞内之高基體。查得在绝食期内副睾上皮细胞之高基體逐渐減少,由网状體变为颗粒,先集于前端後卽散开,至三十六小时高基體绝少,再餵後復有颗粒出现於前端。 (2)输精管上皮细胞内高基體原作线状,列於细胞前部。绝食十三时後,变为颗粒。俟三十六小时後再餵,卽有许多小粒在细胞前端边缘或卽係高基體 (3)由此结果可知高基體之多寡,既视绝食与否而定,或可谓为食物同化之产物,非固定之细胞器官也。 (4)高基體之起源或与粒线體,及分泌粒同,但其所含类脂質之量不同。

(1)A large amount of polyol was found in the mycelium of G. candidum and identified as mannitol by paper chromatography, paper electrophoresis and melting point determinations of the recrystallized polyol and its tetra-acetate derivative.(2)A NADP-specific mannitol dehydrogenase was found in the cell-free extracts of G. candidum, it catalyzed the oxidation of mannitol to fructose by NADP. The reaction was reversible.(3)A phosphatase(or phosphatases)catalyzing the hydrolysis of fructose phosphate esters...

(1)A large amount of polyol was found in the mycelium of G. candidum and identified as mannitol by paper chromatography, paper electrophoresis and melting point determinations of the recrystallized polyol and its tetra-acetate derivative.(2)A NADP-specific mannitol dehydrogenase was found in the cell-free extracts of G. candidum, it catalyzed the oxidation of mannitol to fructose by NADP. The reaction was reversible.(3)A phosphatase(or phosphatases)catalyzing the hydrolysis of fructose phosphate esters to free fructose and inorganic phosphate was also found in the cell-free extracts.(4)No NAD(P)H_2-fructose-6-phosphate reductase activity was detected in the cellfree extracts.(5)The pathway of mannitol formation in G. candidum was established as:D-Fructose-phosphate→Pi→D-Fructose→NADPH_2 NADP→D-Mannitol(6)In xylose-grown mycelium of G. candidum a NAD-specific L-iditol dehydrogenase was adaptively formed, it catalyzed the following reactions:Xylitol→NAD NADH_2→D-Xylulose and D-Sorbitol→NAD NADH_2→D-Fructose.

(1)白地霉菌絲中含有大量的多元醇,經紙层析、紙电泳及提純結晶制备衍生物等方法鉴定为甘露醇。(2)白地霉无細胞提取液中有NADP-甘露醇脫氫酶存在,催化甘露醇被NADP氧化为果糖或相反地果糖被NADPH_2还原为甘露醇。(3)白地霉无細胞提取液中有磷酸酯酶,催化果糖磷酸酯水解为自由果糖及无机磷酸。(4)白地霉中測不出果糖-6-磷酸还原酶活力。(5)确定了甘露醇的形成途径为: D-果糖磷酸→Pi→D-果糖←NADPH_2 NADP→D-甘露醇(6)D-木糖培养的白地霉适应形成了NAD-艾杜糖醇脫氫酶,催化: 木糖醇←NAD NADH_2→D-木酮糖D-山梨醇←NAD NADH_2→D-果糖

The cell-free extract of Streptomyces griseus inhibits strongly the dehydrogenase systems, using NAD or NADP as hydrogen acceptor. Experiments showed that the extract contains a very active NAD(P)nucleosidase which rapidly hydrolyzes NAD(P), thus renders the dehydrogenation impossible to proceed. It accounts for the failure of repeated efforts to demonstrate any NAP(P)-linked dehydrogenase activity in the cell-free extract of this microorganism.The enzyme from Streptomyces griseus differs from that...

The cell-free extract of Streptomyces griseus inhibits strongly the dehydrogenase systems, using NAD or NADP as hydrogen acceptor. Experiments showed that the extract contains a very active NAD(P)nucleosidase which rapidly hydrolyzes NAD(P), thus renders the dehydrogenation impossible to proceed. It accounts for the failure of repeated efforts to demonstrate any NAP(P)-linked dehydrogenase activity in the cell-free extract of this microorganism.The enzyme from Streptomyces griseus differs from that of animals and of other microorganisms. It is closely associated with nucleic acid, and is very stable to heat and to some protein-denaturing agents. After removal of the nucleic acid, the thermostability of the enzyme decreased markedly. Readdition of nucleic acid or its degradation products restores the thermostability. Nucleic acid also affects the pH response of the enzyme. Between pH 5.5—9.0, enzyme activity remains almost equal, yet after the removal of nucleic acid, a pH optimum of 8.0—8.5 appears.

发現鏈霉菌的細胞抽出液能够抑制以NAD或NADP作为受氫体的脫氫酶的反应系統中还原型輔酶的形成。实驗証明,这种抑制作用的原因是細胞抽出液中存在有菸酰胺核苷酸酶,它能迅速地水解菸酰胺輔酶,而使脫氫酶无法表現其活力。这一发現足以解析为什么在鏈霉菌的抽出液中一直不能測出各种脫氫酶的活力来。鏈霉菌的菸酰胺核苷酸酶与已知各种来自动物或微生物的菸酰胺核苷酸酶不同,鏈霉菌中酶的特点是: (1)經純化以后,酶制剂中仍含有核酸。(2)对某些蛋白貭变性剂与对热非常稳定,在除去酶制剂中的DNA以后,其对热稳定性即行降低;向已去核酸的酶中加入核酸或其分解产物,可以使其对热稳定性恢复、甚至超过原来的水平。(3)在pH5.5—9.0之間,酶活力相等,但在去除酶制剂中的核酸以后,出現了酶活力的最适pH值。

 
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