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   in the cells 在 基础医学 分类中 的翻译结果: 查询用时:0.886秒
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in the cells    
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  组细胞
    Quantitative analysis showed that the activities of AKP in the cells of the liver matrix group (the liver matrix P< 0. 01) and the collagen group (the collagen group P<0. 05) were higher than those in the control group in two weeks.
    定量分析显示,培养14d内,肝基质组与鼠尾胶组细胞AKP活性明显高于对照组(肝基质组P<0.01;鼠尾胶组P<0.05);
短句来源
    Cultured for 21 days later, the activities of AKP in the cells of liver matrix group were stronger than those in the collagen group and the control (P <0. 01).
    培养21d后,肝基质组细胞AKP活性分别高于鼠尾胶组和对照组(P<0.01);
短句来源
    Results Typical morphological features of DC were found in the cells of both the two groups by inverse microscopy and electron microscopy. High expression levels of CD1α, HLA-DR, CD80, and CD40 were also found in the cells of the both groups.
    结果 两组细胞在倒置显微镜和扫描电镜下均显示典型的树突状细胞形态 ,流式细胞仪分析均高表达CD1α、HLA DR、CD80和CD4 0分子 ;
短句来源
    The contents of HBV DNA in the cells were detected by fluorescent quantitative polymerase chain reaction (FQ-PCR).
    荧光定量PCR检测各组细胞HBV DNA含量.
短句来源
    In the 1st week,little hepatin was detected in the cells of both groups and the content of carbohydrate in the cells of group 1 was higher than that in cells of the groups 2(u = 7.10,P < 0.01).
    培养第1周,2组细胞几乎均不含糖原,但实验1组细胞的糖水平高于实验2组,差异有统计学意义(u=7.10,P<0.01)。
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  在细胞
    Subsequently all the corresponding proteins were expressed separately in the presence of IPTG, and purified by using affinity chromatography. Meanwhile all the cloned DNA fragments were inserted into the eukaryotic expression vector pcDNA3.1(+) in sense orientation, and transfected into COS-7 cells, and the expression of the target proteins in the cells were identified by immunocytochemistry.
    同时将以上所克隆的各DNA片段分别构建于真核表达载体pcDNA3.1(+),并转染于COS-7细胞,经免疫细胞化学法证实各目的蛋白在细胞中的表达。
短句来源
    The activity of protein kinase C in the liver of dogs was determined 4 hours follwing endoxin administration. The results showed that the activity of both cytosolic and membrane-associated protein kinase C was significantly reduced,and the ratio of the membrane-associated activi- ty to total activity in the cells was also reduced.
    静脉注射内毒素后4小时,狗肝脏细胞浆和细胞膜蛋白激酶 C 活性显著降低,细胞膜蛋白激酶 C 在细胞蛋白激酶 C 总活性中的比例显著减少。
短句来源
    Methods The dominant negative allele of ERK1 was stably overexpressed in the cells and cellular survival assays were performed by using colony formation analysis. ?
    方法 在细胞中稳定表达ERK1及显性失活等位基因ERK1(ERK1-KR) ,然后用克隆形成分析法测定细胞在严重热休克时的存活能力。
短句来源
    Then, HCV RNA of the cultured cells and supernatants was detected by reverse transcriptase polymerase chain reaction (RT PCR) every month and in situ PCR to identify the location of HCV RNA in the cells.
    而后 ,每个月应用逆转录 套式 聚合酶链反应 (RT net PCR)检测 1次培养细胞和上清液中的HCVRNA ,并用原位RT net PCR观察HCVRNA在细胞中存在的部位 ;
短句来源
    BHK\-\{21\},LLC MK\-2, A\-\{549\}, and C6/36 were selected for generation transferring cells to examine the sensitivity of the viruses. The CPE appearance time, characteristics, and titer of the viruses in the cells was observed to carry out the sensitivity testing of the five viruses.
    选择BHK2 1、LLC MK2 、A549和C6 / 36传代细胞 ,进行这 5种病毒的敏感性试验 ,观察病毒在细胞上的CPE出现时间、特点以及滴度。
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  在细胞内
    In this research,gene fusion of cholera toxin B subunit with spike protein,nucleocaspid protein or small membrane protein was obtained,and fusion protein shold be expressed in the cells being transfected with the recombinant plasmid.
    通过将SARS冠状病毒免疫保护相关的核衣壳蛋白和辐条蛋白与霍乱毒素B亚基基因融合,并克隆至真核基因表达载体,所构建的DNA候选疫苗在细胞内可表达霍乱毒素B亚基与SARS抗原的融合蛋白,从而可用于评价是否可有效产生粘膜免疫。
短句来源
    Viral plaque forming assay and MTT assay were used to detect the viral titer change and protective function of the pshRNA7816 on RSV infected Hep2 cell. Results The recombinant RNAi plasmid pshRNA7816 which targets the mRNA of RSV M2-1 gene was successfully constructed. The pshRNA7816 significantly reduced CPE of RSV infected Hep2 cells,reduced the viral titer of RSV in the cells(P<0.001).
    结果成功构建了针对RSV M2-1基因mRNA的pshRNA7816载体质粒,研究发现pshRNA7816能明显改善RSV所致的病变效应,降低RSV在细胞内复制的病毒滴度,并且能明显提高被RSV感染后细胞的存活率(P<0.001),非特异性质粒无抗RSV效应。
短句来源
    At the early stage of the formation, the corpuscles appeared to be secretory granules in the cells.
    在形成的早期,石灰小体以分泌颗粒的形式出现在细胞内
短句来源
    In contrast, only EGF P expression could be detected in K562 cells transfected with empty vector with diffuse distribution in the cells.
    相反 ,转染空载体的细胞仅表达EGFP ,且其在细胞内呈弥散样分布。
短句来源
  后细胞
    RESULTS: The result show that hypoxia and reperfusion increased the necrosis rate and apoptosis rate, enhanced the concentration of LDH in the medium and the [Ca 2+]i overload in the cells (P<0.05), but there was no difference between these groups (P>0.05).
    结果:实验表明,缺氧复氧损伤可以造成不同部位间心肌细胞坏死率和凋亡率明显升高,培养液中LDH的含量增加,细胞内钙离子超载,但各组细胞间并没有明显区别(P>0.05)。 给予缺氧预适应后,细胞损伤的各种指标均显著低于缺氧复氧组(P<0.05)。
短句来源
    The levels of IL-2 and IFN-γ se- cretion enhanced significantly in the cells with lactadherin functional peptide in comparison with those in the untreated cells (P = 0.0394, P = 0.0082, respectively), but IL-4 secretion had no marked change.
    给予乳凝集素处理后细胞培养上清中IL-2(P=0.0394)和IFN-γ(P=0.0082)的含量高于未处理细胞组,而IL-4没有显著的增高;
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  in the cells
Enzyme activity was revealed in the cells grown on medium with elemental sulfur or in the presence of various sulfide minerals and concentrates of sulfide ores.
      
The mutants varied in Cr(VI) resistance, the degree of chromium accumulation in the cells (from 0.1 to 11.6 mg/g dry cells), and the degree of Cr(VI) reduction (from 50% to complete disappearance of bichromate from the culture liquid).
      
Treatment with ambiol enhanced the differentiation of the internal membrane system of plastids in the cells of original and transgenic plants, especially the tubular membrane systems.
      
The concentration of eight kinds of catechins in solution decreased by 29.6-47.6%, respectively; some catechins were absorbed and accumulated by yeast cells, but the amount in the cells was very low during the fermentation process.
      
Specific attention is given to regulation of enzymes of antioxidative defense, operating in the cells of strict anaerobes under the conditions of oxidative stress caused by oxygen, superoxide anion, or hydrogen peroxide.
      
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Acute experiments were carried out on rabbits with the pancreatic duct ligated from one day to two months previously. The change of the concentration of free fatty acid (FFA) in the plasma was observed after electrical stimulation of the peripheral ends of the dorsal and ventral subdiaphragmatic vagus nerves and a correlated histological and histochemical examination of the pancreatic tissue was also undertaken. The results were as follows: The concentration of the plasma FFA in normal rabbits was markedly increased...

Acute experiments were carried out on rabbits with the pancreatic duct ligated from one day to two months previously. The change of the concentration of free fatty acid (FFA) in the plasma was observed after electrical stimulation of the peripheral ends of the dorsal and ventral subdiaphragmatic vagus nerves and a correlated histological and histochemical examination of the pancreatic tissue was also undertaken. The results were as follows: The concentration of the plasma FFA in normal rabbits was markedly increased after vagal stimulation. After ligation of the pancreatic duct, however, the result of stimulation gradually changed with the length of the ligation period: In the first 2 days after ligation, both increase and decrease were observed with about the same frequency; 3—21 days after ligation, the result was more frequently a decrease; 23—60 days after ligation, the effect was uniformly a decrease. Histological and histochemical examination revealed that there was a great deal of degeneration in the parenchyma after ligation of the pancreatic duct and that about 3 weeks after ligation the acinar cells had atrophied and there was an abundant infiltration of fibrous tissue. The lipase granules fn the acinar cells were present after 2 days of ligation. From the third day on, the lipase reaction began to disappear entirely. The alkaline phosphatase reaction was still positive in the cells of the dilated ductules and the islet cells remained normal after 2 months of ligation. It is supposed that the increase and decrease of the plasma FFA after the vagal stimulation might be due respectively to two separate factors in the pancreas. The factor that causes the increase and disappears after atrophy of the parenchyma, might come from the exocrine system of the organ, while the origin of the factor which causes the decrease is not yet clear. Further work is obviously needed in this connection in order to elucidate the exact mechanism.

本工作以兎为实驗对象,在結扎胰导管后1—60日等不同日期,进行急性实驗,电刺激腹、背侧膈下迷走神經外周端,观察血浆自由脂肪酸濃度和胰腺組織学及組織化学的变化。結果如下: 刺激未結扎胰导管兎的膈下迷走神經外周端,血浆自由脂肪酸濃度表現为升高反应。結扎胰导管后不同日期刺激迷走神經,則反应形式发生了明显的阶段性变化:在結扎的初期(1、2日),除升高反应外,有的还表現有降低反应;随着結扎日期的延长(3—21日),仅偶尔出現升高反应,而多数則表現为波动及降低反应;在結扎胰导管3周后(23—60日),全部表現为降低反应。 組織学和組織化学檢查显示:結扎胰导管后,胰腺腺泡細胞逐漸破坏,至3周左右,一般都已消失,代之以增生的結締組織。腺泡細胞內脂肪酶于結扎后1、2日內仍呈阳性反应,而于3日后郎呈阴性反应。导管系統在結扎后卽行扩張,小导管壁硷性磷酸酶在結扎后60日內均呈阳性反应。胰島細胞在結扎胰导管2月內仍正常。根据以上結果,似可推想,兴奋迷走神經有使血浆自由脂肪酸濃度升高和降低的两种作用。在不結扎胰导管情况下,刺激迷走神經所引起血浆自由脂肪酸濃度的升高反应,可能是由于升高作用占优势;結扎胰导管后,在胰腺腺泡細胞逐漸萎縮的情况...

本工作以兎为实驗对象,在結扎胰导管后1—60日等不同日期,进行急性实驗,电刺激腹、背侧膈下迷走神經外周端,观察血浆自由脂肪酸濃度和胰腺組織学及組織化学的变化。結果如下: 刺激未結扎胰导管兎的膈下迷走神經外周端,血浆自由脂肪酸濃度表現为升高反应。結扎胰导管后不同日期刺激迷走神經,則反应形式发生了明显的阶段性变化:在結扎的初期(1、2日),除升高反应外,有的还表現有降低反应;随着結扎日期的延长(3—21日),仅偶尔出現升高反应,而多数則表現为波动及降低反应;在結扎胰导管3周后(23—60日),全部表現为降低反应。 組織学和組織化学檢查显示:結扎胰导管后,胰腺腺泡細胞逐漸破坏,至3周左右,一般都已消失,代之以增生的結締組織。腺泡細胞內脂肪酶于結扎后1、2日內仍呈阳性反应,而于3日后郎呈阴性反应。导管系統在結扎后卽行扩張,小导管壁硷性磷酸酶在結扎后60日內均呈阳性反应。胰島細胞在結扎胰导管2月內仍正常。根据以上結果,似可推想,兴奋迷走神經有使血浆自由脂肪酸濃度升高和降低的两种作用。在不結扎胰导管情况下,刺激迷走神經所引起血浆自由脂肪酸濃度的升高反应,可能是由于升高作用占优势;結扎胰导管后,在胰腺腺泡細胞逐漸萎縮的情况下,升高作用逐渐消失,从而使降低作用占优势。但有关的詳細机制,尚有待于进一步深入研究。

Forty-nine adult male albino rats, each weighing from 140—210 gms, were selected for this experiment. Out of them, 9 were left without any treatment for control, and each of the rest 40 experimental animals received daily intramuscular injection of 25 mg. of cortisone or hydrocortisone. The experimental animals were sacrificed 24 hr. after 1, 3, and 6 injections and on 4, 5, and 9 days after the 6th injections. The control animals were killed at the same time intervals. The spleen of these animals were immediately...

Forty-nine adult male albino rats, each weighing from 140—210 gms, were selected for this experiment. Out of them, 9 were left without any treatment for control, and each of the rest 40 experimental animals received daily intramuscular injection of 25 mg. of cortisone or hydrocortisone. The experimental animals were sacrificed 24 hr. after 1, 3, and 6 injections and on 4, 5, and 9 days after the 6th injections. The control animals were killed at the same time intervals. The spleen of these animals were immediately fixed for both histological and histochemical studies. With repeated injections, as the time advanced, the spleen gradually atrophiel; after the 6th injection or 4 and 5 days after it, its size reached only 1/3 of the normal. Correspondingly, its internal structures showed conspicuous changes. Lymphocytes in the red and white pulps decreased in number, and almost vanished on 4 and 5 days after the last injections. The whole spleen was almost entirely occupied by the red pulp, the white pulps were difficult to identify. These changes tended to recover on the 9th day after the last injection, but never reached the normal level. The content of RNA decreased in lymphocytes of the spleens of the experimental animals, especially in the large and median sized lymphocytes. Pyknotic and fragmented lymphocyte nuclei were universally observed, especially in the Malpigian corpuscles; some were found in phagocytes. Granulocytes accumulated at the periphery of the Malpigian corpuscles. There was an increased amount of macrophages and their engulfed hemosiderin. Proteins and protein-bound SH groups in the cell elements of the spleen of the experimental animals showed no obvious changes in content, but due to the decrease in number of lymphocytes, their total amount in spleen was decreased. The changes of acid and alkaline phosphatases after the injections of cortisone were evident. The activity of ALP showed an initial elevation, reaching the maximum after 3 injections, and then followed by a continuous fall. The activity of ACP, on the other hand, showed consistent increasing, and was most prominent on the 4th and 5th days after the last injections. The former chiefly resided in neutrophils and latter in reticular cells. The activity of adenosine triphosphatase elevated first after injections, but gardually dropped to normal level. 5-Nucleotidase, non-specific esterase and non-specific phosphatase showed little or no changes during the course of administration of cortisone.

一、实验共用成年雄性大白鼠49只,体重为140-210克。其中9只不加任T可处理作为对照。其余40只分别经臀部肌肉注射可的松或氢化可的松25毫克/每日一次。注射分一次、三次、六次以及注六次后恢复4,5和9日后杀死取材。二、随注射次数的增加和实验时间的延长大鼠脾脏逐渐萎缩。到注六次或注六次后恢复4和5日时可缩小到正常的1/3以上。其内部结构也相应地引起了十分明显地变化。红白髓中淋巴细胞迅速减少,到注六次恢复4和5日时脾小体几乎完全消失,红白髓难于辨认。此时整个脾脏几乎被红髓占据。注射六次后恢复9日时,虽有些恢复,但仍未达正常水平。三、实验动物脾脏淋巴细胞中RNA合量减少,尤其大中淋巴细胞较为明显。固缩和破碎的淋巴细胞核普遍存在,尤其脾小体处为多,有些已被亘噬细胞吞噬。而中性粒细胞的数量增加,井多量的堆积于脾小体周围。旦噬细胞及其吞噬的含铁血黄素显著增多。 蛋白质、蛋白质结合一SH和DNA含量未见变化。四、磷酸酶的活性变化,以ALP和ACP较为显著。ALP在注射初期升高,以注射三次者为最明显,随后酶活性一值下降。而ACP活性在实验时期一直上升。前者以中性粒细胞为代表,后者以网状细胞为代表。三磷酸腺普酸酶在注...

一、实验共用成年雄性大白鼠49只,体重为140-210克。其中9只不加任T可处理作为对照。其余40只分别经臀部肌肉注射可的松或氢化可的松25毫克/每日一次。注射分一次、三次、六次以及注六次后恢复4,5和9日后杀死取材。二、随注射次数的增加和实验时间的延长大鼠脾脏逐渐萎缩。到注六次或注六次后恢复4和5日时可缩小到正常的1/3以上。其内部结构也相应地引起了十分明显地变化。红白髓中淋巴细胞迅速减少,到注六次恢复4和5日时脾小体几乎完全消失,红白髓难于辨认。此时整个脾脏几乎被红髓占据。注射六次后恢复9日时,虽有些恢复,但仍未达正常水平。三、实验动物脾脏淋巴细胞中RNA合量减少,尤其大中淋巴细胞较为明显。固缩和破碎的淋巴细胞核普遍存在,尤其脾小体处为多,有些已被亘噬细胞吞噬。而中性粒细胞的数量增加,井多量的堆积于脾小体周围。旦噬细胞及其吞噬的含铁血黄素显著增多。 蛋白质、蛋白质结合一SH和DNA含量未见变化。四、磷酸酶的活性变化,以ALP和ACP较为显著。ALP在注射初期升高,以注射三次者为最明显,随后酶活性一值下降。而ACP活性在实验时期一直上升。前者以中性粒细胞为代表,后者以网状细胞为代表。三磷酸腺普酸酶在注射初期上升,之后则逐渐恢复正常。5一核试酸酶、非特异性磷酸酶和醋酶的活性虽有变化,但不明显。

The cytoskeletal element in several kinds of cells was shown by the new staining technique with coomassie blue. In normal mouse heart cells, and mouse and human esophageal epithelial cells they appeared as straight fibers usually running along the whole of the cell in parallel arrays. They were diminished in number and irregularly arranged in a network in esophageal carcinoma cells (ECa 109). These filamentous structure became diffused by exposuring of the cells to cytochalasin D or hyperthermic treatment. Aggregation...

The cytoskeletal element in several kinds of cells was shown by the new staining technique with coomassie blue. In normal mouse heart cells, and mouse and human esophageal epithelial cells they appeared as straight fibers usually running along the whole of the cell in parallel arrays. They were diminished in number and irregularly arranged in a network in esophageal carcinoma cells (ECa 109). These filamentous structure became diffused by exposuring of the cells to cytochalasin D or hyperthermic treatment. Aggregation of these filamentous organelles into bundles was seen in the cells treated with maytasin(extract of the stem of Maytenus Hookerii Loas)or colchicine. Cells during mitosis were seen attaching to other cells in interphases by protein fibers. It suggested that the cytoskeleton revealed by coomassie blue may be the microfilament bundles seen by immunofluorescence with anti-actin.

用考马斯蓝染色法显示了几种体外培养细胞的细胞骨架。在正常细胞中,如小鼠心肌细胞、小鼠食管上皮细胞以及人正常食管上皮细胞都有贯穿整个细胞并与细胞表面平行的纤维状结构。人食管癌上皮细胞中的纤维状结构少,而且排列不规则,形成网状。这些纤维状骨架受细胞松弛素D和高温(42.5℃与44.5℃)作用后呈弥散状。受美登木素或秋水仙素作用后仍然有部分存在,并有集合成索的现象。分裂细胞有蛋白质支架与其它细胞相连。讨论了用考马斯蓝显示的细胞骨架与肌动蛋白的关系。

 
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