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mannan-binding
相关语句
  甘露聚糖结合
     Construction of CGT52TGT and GGA57GAA mutants of mannan-binding lectin gene
     CGT52TGT和GGA57GAA甘露聚糖结合凝集素突变体的构建
短句来源
     Objective To investigate the effects of mannan-binding lectin (MBL) on TNF-α and IL-12 production induced by lipopolysaccharide (LPS) in differentiated and activated THP1/CD14 cells.
     目的探讨甘露聚糖结合凝集素(MBL)对脂多糖(LPS)刺激的不同分化和活化状态THP1/CD14细胞产生TNF-α和IL-12的影响。
短句来源
     Objective To investigate the frequencies of three point mutations,CGT52TGT,GGC54GAC and GGA57GAA,in exon 1 of mannan-binding lectin(MBL)structural gene in Chinese Uyghur population.
     目的了解我国维吾尔族人群甘露聚糖结合凝集素(MBL)结构基因外显子1第52、54和57位密码点突变(CGT52TGT、GGC54GAC和GGA57GAA)的情况。
短句来源
     Objective To construct two mutants of human mannan-binding lectin (MBL) gene, CGT52TGT and GGA57GAA.
     目的 构建CCT52TGT和GGA57GAA 2种甘露聚糖结合凝集素(MBL)基因突变体。
短句来源
     Objective To obtain full-length cDNA encoding mouse mannan-binding lectin C(MBL-C) polypeptide.
     目的获得小鼠甘露聚糖结合凝集素-C(MBL-C)基因的全长编码区cDNA。
短句来源
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  “mannan-binding”译为未确定词的双语例句
     Effects of mannan-binding lectin on TNF-α and IL-12 production induced by lipopolysaccharide in differentiated and activated THP1/CD14 cells
     MBL对LPS刺激的不同分化和活化状态THP1/CD14细胞产生TNF-α和IL-12的影响
短句来源
     Construction of GGC54GAC mutant of mannan-binding lectin
     GGC54GAC MBL突变体的构建
短句来源
     AIM: To explore preliminarily the mechanisms of immunodeficiency resulted from the CGT52TGT point mutation of mannan-binding lectin(MBL) gene.
     目的: 初步探索MBL基因CGT52TGT点突变引起调理吞噬缺损的机制。
短句来源
     Frequency of GGC54GAC point mutation of mannan-binding lectin gene in a Han population from Guangdong
     广东地区汉族人MBL基因GGC54GAC点突变的初步筛查
短句来源
     Objective To construct the standard recombinant plasmids for 7 common haplotypes of mannan-binding lectin (MBL) gene.
     目的构建7种常见的MBL基因单倍型标准质粒。
短句来源
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  相似匹配句对
     Electron microscope observation of Mannan
     酵母甘露聚糖的电子显微镜观察
短句来源
     Determination of Mannan in Coconut Shell
     椰子壳中甘露聚糖含量的测定
短句来源
     Mannan-Binding Lectin and Infectious Diseases
     甘露糖结合凝集素与感染性疾病
短句来源
     Purification and Characterization of Mannan-binding Lectin
     甘露聚糖结合凝集素的分离纯化和活性研究
短句来源
     Review of Separation and Purification of Konjac Gluco-mannan
     魔芋葡甘露聚糖的分离纯化方法综述
短句来源
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  mannan-binding
Possible disease-modifying factors: the mannan-binding lectin pathway and infections in hereditary angioedema of children and ad
      
In contrast, two mannose-specific lectins, the mannan-binding protein (MBP) and serum amyloid P component (SAP), isolated from human serum, have inhibitory effects, both in the absence and presence of EGF.
      
Mannan-binding lectin (MBL), a human plasma protein, plays an important role in the innate immune defence.
      
Phase I Safety, Tolerability, and Pharmacokinetic Study of Recombinant Human Mannan-Binding Lectin
      
Mannan-binding lectin (MBL) is an activator of the complement system, and Apolipoprotein A-1 (Apo A-1) is pivotal in the cholesterol homeostasis and has anti-inflammatory properties.
      
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Pinellia ternata lectin (PTL) can bind to yeast mannan. In this paper we examined the effect of some cations on mannan-binding activity of PTL, and investigated systematically the carbohydrate-binding specificity of PTL. Data showed that cations and EDTA had no effect on mannan-binding activity of PTL, except that potassium ion enhanced 61% of the binding activity of PTL at 2mmol/L.A variety of monosaccharides and disaccharides so far used could not inhibit the binding of PTL with manuan, but glycosides...

Pinellia ternata lectin (PTL) can bind to yeast mannan. In this paper we examined the effect of some cations on mannan-binding activity of PTL, and investigated systematically the carbohydrate-binding specificity of PTL. Data showed that cations and EDTA had no effect on mannan-binding activity of PTL, except that potassium ion enhanced 61% of the binding activity of PTL at 2mmol/L.A variety of monosaccharides and disaccharides so far used could not inhibit the binding of PTL with manuan, but glycosides with hydrophobic aglycone displayed prominent inhibition at the concentration ranging from 0.5 to 2mmol/L. It is shown that PTL bound specifically with the glycoconjugates carrying high-mannose oligosaccharide chain (Man_6GlcNAc_2).

半夏凝集素(PTL)可与甘露聚糖结合。本文以PTL与~(125)I标记的甘露聚糖的结合活性为指标,观察了一些金属离子对PTL的糖结合活性的影响,井对PTL的糖结合专一性作了较系统的研究。结果表明常见的金属离子或EDTA对其糖结合活性无显著影响,但K~+可明显增加PTL的糖结合活性。大多数单糖,二糖不抑制PTL与甘露聚糖的结合,但一些疏水配基形成的糖苷可产生显著的抑制效应。PTL专一与高甘露糖型糖链结合。

Mannanbinding lectin(MBL)was isolated from human plasma by affinity chromatography on mannanSepharose 4B.It appears as a single band with an apparentmolecular weight of 29 000 on SDSPAGE under reducing conditions.Binding of 125Imannan to MBL is concentrationdependent,saturable and inhibited by Nacetylmannosamine,Nacetylglucosamine,mannose,or Lfucose but not by glucose,galactose,or other sugars.125Imannan binding to MBL is Ca2+dependent,with 50% of maximal binding at approximately 1mmol/L CaCl2....

Mannanbinding lectin(MBL)was isolated from human plasma by affinity chromatography on mannanSepharose 4B.It appears as a single band with an apparentmolecular weight of 29 000 on SDSPAGE under reducing conditions.Binding of 125Imannan to MBL is concentrationdependent,saturable and inhibited by Nacetylmannosamine,Nacetylglucosamine,mannose,or Lfucose but not by glucose,galactose,or other sugars.125Imannan binding to MBL is Ca2+dependent,with 50% of maximal binding at approximately 1mmol/L CaCl2.

应用甘露聚糖-Sepharose4B柱亲和层析,从人血浆中分离纯化了甘露聚糖结合凝集素(MBL)。在还原条件下进行SDS-PAGE,该结合蛋白显示29KD的单一条带。125I-甘露聚糖与MBL的结合是浓度依赖、可饱和的,且受N-乙酰甘露糖胺、N-乙酰葡糖胺、甘露糖及L-岩藻糖的抑制,但葡萄糖、半乳糖及其它单糖则否。这种结合依赖Ca2+,达50%最大结合量的Ca2+浓度大约为1mmol/L。

The intact cDNA encoding the mannan binding lectin polypeptide including the signal sequence was isolated by using RT PCR method with total RNA extracted from fresh liver tissue of a Han nationality foetus, linked into pGEM T vector and transformed into E.coli TG1. The result of sequencing indicated that the sequence of our cDNA is different greatly from that of the reported human MBL cDNA but identical with that of the mRNA for human...

The intact cDNA encoding the mannan binding lectin polypeptide including the signal sequence was isolated by using RT PCR method with total RNA extracted from fresh liver tissue of a Han nationality foetus, linked into pGEM T vector and transformed into E.coli TG1. The result of sequencing indicated that the sequence of our cDNA is different greatly from that of the reported human MBL cDNA but identical with that of the mRNA for human MBL in GenBank except two nuc leotides, and that its encoded product consists of a typical hydrophobic signal sequence of 20 amino acids and a mature MBL polypeptide of 228 amino acids.

从中国汉族人胎肝组织提取总RNA,以RT-PCR方法获取了编码含信号顺序的全长甘露聚糖结合凝集素(MBL)肽链的cDNA片段,将其与pGEM-T载体连接,转化大肠杆菌TG1,建立了MBL的cDNA克隆。序列分析表明:与文献报道的人MBLcDNA序列比较,差异颇大,但与GenBank中的人MBLmRNA比较,仅2个位置的核苷酸不同;其编码产物是20个残基的信号顺序和228个残基的成熟MBL多肽。

 
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