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normal media
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  正常培养基
     the cell numbers did not significantly increase after the cells were treated for 24 h and then replaced by culture in normal media for 96 h.
     ③经BPF提取物处理24h后,即使更换为正常培养基,人肝癌细胞数在96h的恢复培养中基本无增加。
短句来源
     (3) Had been treated for 1 week then cultured in normal media for 3 weeks, the human RRP-TB cells slowly increased in number.
     (3)经ACVE处理1 wk后,在更换为正常培养基进行恢复培养过程中,RRP-TB细胞数呈现缓慢增长。
短句来源
     (3)Had been treated for 24 h then cultured in normal media for 72 h,the human hepatoma cells slowly increased in number;
     3经SSCC提取物处理24h后,在更换为正常培养基恢复培养过程中,细胞数呈现缓慢增长;
短句来源
     Results DCN extracts could were treated for 24h and then replaced by cultured in normal media for 96h, the cells entered immedeately into logarithmic phase after the drug was removed from the medium.
     (2)经DCN提取物处理24h后的人肝癌细胞,既使更换为正常培养基,其线粒体代谢活性及细胞增殖与对照相比仍差异显著。
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  “normal media”译为未确定词的双语例句
     Since the normal media is not very effective on this condition, then synthetic diamond factory, as the semi finished product enterprise, have to take cyber-marketing and international trade by building their cyber-marketing system to fight a way in domestic market.
     人造金刚石企业作为典型专用性中间产品的生产企业在其他广告媒体不能起到有效促销作用的情况下,进行网络营销体系的建设并通过网络营销体系开展网上营销和对外贸易从而开拓国际市场并从国内的恶性价格战中突围就成了金刚石企业的最好选择;
短句来源
     d NBP and l NBP(0 1~100 μmol·L -1 ) concentration dependently increased 6 keto PGF 1α release, decreased TXB 2 release from neuronal cells, and significantly enhanced extracellular 6 keto PGF 1α /TXB 2 ratio in primary cultured rat cortical neurons exposed to hypoxic hypoglycemic media for 5 h or hypoxic hypoglycemic media for 5 h following normal media for 3 h.
     用放射免疫方法,结果发现神经细胞在低糖低氧5h或低糖低氧5h/恢复糖氧3h条件下,d,l和dlNBP(01~100μmol·L-1)能够剂量依赖性升高细胞外液中的6酮PGF1α含量,降低TXB2水平,从而使6酮PGF1α与TXB2比值升高。
短句来源
     METHODS: Human normal esophageal epithelial cells cultured in vitro were exposed to media containing 6 different kinds of bile acids (250 μmol/L) respectively for 3-60 min,in comparison with the control group (in normal media without bile acid).
     方法体外培养人正常食管黏膜上皮细胞,分别在含有6种不同胆酸(250μmol/L)的培养液中培养3~60min,并设对照(培养液不含胆酸)进行比较.
短句来源
     2. The wave reflection and refraction on the boundary between a normal media and a 1DLHM, whose dispersion relation is with a hyperbolic k-surface, are studied.
     2.在具有双曲线色散特性的一维各向异性异向介质界面上,当双曲线的渐近线垂直于界面时,平面波的折射反射行为只有在介质被考虑为有损的情况下才能得到合理的解释。
短句来源
     The experiments of effects of SSCC extracts on the proliferation were performed with the treatments of SSCC extracts at dose of 500 μg/ml for 7 d and the proliferation of human hepatoma cells were also examined after treated with SSCC extracts for 24 h and then replaced by cultured in normal media(containing 10% FCS) for 72 h.
     人肝癌SMMC-7721细胞经SSCC提取物处理不同时间后,以MTT法测SSCC提取物对人肝癌细胞线粒体代谢活性的影响,以细胞记数法观察了其对细胞增殖的影响。
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  相似匹配句对
     Media
     媒体互动
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     Media
     媒体
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     Ways and Normal forms to the Research of Media Economy
     试论我国传媒经济的研究
短句来源
     Construction of Normal Vector Wave Functions in Graded Media
     渐变折射率介质中标准矢量波函数的构成
短句来源
     The X chromosome was normal.
     X染色体无变化。
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  normal media
Neither 5 mM lysine or glutamine which abolished arginine transport through systems y+ and B0,+, respectively, reduced nitrite release in cells incubated in normal media.
      
Ca withdrawal increased the efflux of exogenous GABA, primary amines, and TCA-precipitable radioactivity but not of TCA-soluble radioactivity when normal media were used.
      
The release of enzyme activity from endothelial cells was inversely proportional to calcium concentrations ranging between 0.01 mM (activity equivalent to calcium-free media) and 0.5 mM (activity equivalent to normal media).
      
The release of enzyme activity from endothelial cells was inversely proportional to calcium concentrations ranging between 0.01 mM (activity equivalent to calcium-free media) and 0.5 mM (activity equivalent to normal media).
      
Salt tolerance in this culture was characterizedby an altered growth behavior, reduced cell volume, and accumulation of Na+, Cl-, proline and sugars when grown under salt stress, as well as on normal media.
      
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The effects of 3 n butylphthalide(NBP) on the levels of 6 keto PGF 1α , TXB 2 and 6 keto PGF 1α /TXB 2 ratio were studied with methods of RIA. d NBP and l NBP(0 1~100 μmol·L -1 ) concentration dependently increased 6 keto PGF 1α release, decreased TXB 2 release from neuronal cells, and significantly enhanced extracellular 6 keto PGF 1α /TXB 2 ratio in primary cultured rat cortical neurons exposed to hypoxic hypoglycemic media for 5 h or hypoxic hypoglycemic...

The effects of 3 n butylphthalide(NBP) on the levels of 6 keto PGF 1α , TXB 2 and 6 keto PGF 1α /TXB 2 ratio were studied with methods of RIA. d NBP and l NBP(0 1~100 μmol·L -1 ) concentration dependently increased 6 keto PGF 1α release, decreased TXB 2 release from neuronal cells, and significantly enhanced extracellular 6 keto PGF 1α /TXB 2 ratio in primary cultured rat cortical neurons exposed to hypoxic hypoglycemic media for 5 h or hypoxic hypoglycemic media for 5 h following normal media for 3 h. Aspirin(0 1~100 μmol·L -1 ) was also shown to inhibit TXB 2 release from cortical neurons in a dose dependent manner. However aspirin only increased 6 keto PGF 1α /TXB 2 ratio at low dose because aspirin inhibited both 6 keto PGF 1α and TXB 2 release simultaneously at large dose(10~100 μmol·L -1 ). This suggests that the action of l NBP, d NBP and dl NBP on the increase of 6 keto PGF 1α /TXB 2 ratio might be one of the mechanisms in which NBP enhanced focal cerebral blood flow and improved ischemic brain damage.

目的旨在观察丁基苯酞(NBP)对神经细胞培养液中6酮PGF1α和TXB2含量及其比值的影响。用放射免疫方法,结果发现神经细胞在低糖低氧5h或低糖低氧5h/恢复糖氧3h条件下,d,l和dlNBP(01~100μmol·L-1)能够剂量依赖性升高细胞外液中的6酮PGF1α含量,降低TXB2水平,从而使6酮PGF1α与TXB2比值升高。而阿司匹林仅在小剂量(01,1μmol·L-1)时能升高6酮PGF1α与TXB2比值,大剂量(10,100μmol·L-1)时无影响。提示:NBP对6酮PGF1α/TXB2比值的升高可能与其增加局部脑血流和改善缺血性脑损伤有关。

Festuca rubra copper tolerant Merlin and sensitive 559 seedings that socked in deionized water for5 d were divided into seven groups, six of which were cultured respeCtively in normal media for 0, 2, 4, 6, 8and 10 d, and correspondingly named as D1, D2, D4, D6, D8 and D10 prior to their exposure to copper at themass concentration (ρ ) of 5 mg. L- 1 in the same media, and the rest one was grown in normal media throughout the culture. About half of each was harvested on 20 d and the others...

Festuca rubra copper tolerant Merlin and sensitive 559 seedings that socked in deionized water for5 d were divided into seven groups, six of which were cultured respeCtively in normal media for 0, 2, 4, 6, 8and 10 d, and correspondingly named as D1, D2, D4, D6, D8 and D10 prior to their exposure to copper at themass concentration (ρ ) of 5 mg. L- 1 in the same media, and the rest one was grown in normal media throughout the culture. About half of each was harvested on 20 d and the others on 27 d. Among these seedlings, Merlin D6 had the highest dry material contents in shoots on 27 d and in roots on 20 d maximum chlorophyll contents on both d 20 and 27 seedlings, minimal copper accumulation in the rootS of seedlings on 27 d, and thetallest shoots in Merlin seedlings on 20 d. FOr seedlings on 20 d, Merlin D6 showed a maximum increase inshoots and maximum decrease in roots of dry mass production, maximum reduction of chlorophyll content andminimal quantities of copper accumulation in roots after the further culture for a week. These results indicatedthat even in the copper tOlerant Merlin, different tolerance to metal might express and a maximal tolerant pointmight exist when Merlin was treated by copper at the different developmental stages.

紫羊茅(Festucarubra)抗铜品种Merlin和敏感品种559种子在水中吸胀5d后长出的幼苗在一般培养液中分别培养了0、2、4、6、8和10d(分别称为D0、D2、D4、D6、D8和D10)后再置于ρ(Cu2+)=5mg/L的相同培养液中,其中约一半培养至d20,其余至d27.结果与同龄的幼苗相比,MerlinD6在27d和20d的根中均有最高的干物质含量和最大的叶绿素浓度;在27d苗的根中铜积累了最少;而MerlinD6的苗在各个抗性处理组中最高在从20d至27d的培养期间,MerlinD6地上部分的干物质增加最多,而根中则减少最多,叶绿素含量也减少最多,在根中积累的铜也最少;可是在敏感的S59中则没有这种特殊现象,这些现象暗示,即使是在抗性品种的Merlin中,在其不同的生长时期经铜处理,也表现出不同的抗性,并可能有一个抗性最大的生长期。

Objective:To dimonstrate the effects of the extracts from Sophora Subprostrata chun et T chan(SSCC) on the proliferation and mitochondria metabolism of human hepatoma SMMC 7721 cells.Methods:After the test of dose dependent experiment,human hepatoma cells were treated with SSCC extracts at dose of 250 μg/ml for 48 h and mitochondria metabolism activity was detected by MTT method.The experiments of effects of SSCC extracts on the proliferation were performed with the treatments of SSCC extracts at dose of 500...

Objective:To dimonstrate the effects of the extracts from Sophora Subprostrata chun et T chan(SSCC) on the proliferation and mitochondria metabolism of human hepatoma SMMC 7721 cells.Methods:After the test of dose dependent experiment,human hepatoma cells were treated with SSCC extracts at dose of 250 μg/ml for 48 h and mitochondria metabolism activity was detected by MTT method.The experiments of effects of SSCC extracts on the proliferation were performed with the treatments of SSCC extracts at dose of 500 μg/ml for 7 d and the proliferation of human hepatoma cells were also examined after treated with SSCC extracts for 24 h and then replaced by cultured in normal media(containing 10% FCS) for 72 h.Result:(1)SSCC extracts could significantly decrease the activity of mitochondria metabolism,and the A570 absorption in the treatment group was only 62.1% of that in control group after 48 h treatment and 9.64% after 72 h treatment.(2)The human hepatoma cell number in the treatment group was much lower than that in control groups during the two groups were treated for seven days.(3)Had been treated for 24 h then cultured in normal media for 72 h,the human hepatoma cells slowly increased in number;(4)After treated for 24 h,the mitosis index in the treatment group was only 50.6% of that in the control group.Conclusion:SSCC extracts could effectively inhibit the proliferation and mitochondria metabolism activty of human hepatoma cells. [

目的:研究中药山豆根(SSCC)提取物对体外培养的人肝癌细胞的抑癌活性。方法:SSCC经粉碎、浸提、超速离心、减压浓缩、冷冻干燥得SSCC提取物。人肝癌SMMC-7721细胞经SSCC提取物处理不同时间后,以MTT法测SSCC提取物对人肝癌细胞线粒体代谢活性的影响,以细胞记数法观察了其对细胞增殖的影响。结果:1经SSCC提取物处理48h,其A570光吸收降为对照组的62.1%,72h为9.64%;2SSCC提取物处理7d后,人肝癌细胞数明显低于对照组;3经SSCC提取物处理24h后,在更换为正常培养基恢复培养过程中,细胞数呈现缓慢增长;4经SSCC提取物处理24h后其有丝分裂指数为对照组的50.6%。结论:中药SSCC提取物能抑制体外培养的人肝癌细胞的增殖,降低线粒体代谢活性。

 
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