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normal media
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  正常培养基
    the cell numbers did not significantly increase after the cells were treated for 24 h and then replaced by culture in normal media for 96 h.
    ③经BPF提取物处理24h后,即使更换为正常培养基,人肝癌细胞数在96h的恢复培养中基本无增加。
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    (3) Had been treated for 1 week then cultured in normal media for 3 weeks, the human RRP-TB cells slowly increased in number.
    (3)经ACVE处理1 wk后,在更换为正常培养基进行恢复培养过程中,RRP-TB细胞数呈现缓慢增长。
短句来源
    (3)Had been treated for 24 h then cultured in normal media for 72 h,the human hepatoma cells slowly increased in number;
    3经SSCC提取物处理24h后,在更换为正常培养基恢复培养过程中,细胞数呈现缓慢增长;
短句来源
    Results DCN extracts could were treated for 24h and then replaced by cultured in normal media for 96h, the cells entered immedeately into logarithmic phase after the drug was removed from the medium.
    (2)经DCN提取物处理24h后的人肝癌细胞,既使更换为正常培养基,其线粒体代谢活性及细胞增殖与对照相比仍差异显著。
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  “normal media”译为未确定词的双语例句
    The experiments of effects of SSCC extracts on the proliferation were performed with the treatments of SSCC extracts at dose of 500 μg/ml for 7 d and the proliferation of human hepatoma cells were also examined after treated with SSCC extracts for 24 h and then replaced by cultured in normal media(containing 10% FCS) for 72 h.
    人肝癌SMMC-7721细胞经SSCC提取物处理不同时间后,以MTT法测SSCC提取物对人肝癌细胞线粒体代谢活性的影响,以细胞记数法观察了其对细胞增殖的影响。
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  normal media
Neither 5 mM lysine or glutamine which abolished arginine transport through systems y+ and B0,+, respectively, reduced nitrite release in cells incubated in normal media.
      
Ca withdrawal increased the efflux of exogenous GABA, primary amines, and TCA-precipitable radioactivity but not of TCA-soluble radioactivity when normal media were used.
      
The release of enzyme activity from endothelial cells was inversely proportional to calcium concentrations ranging between 0.01 mM (activity equivalent to calcium-free media) and 0.5 mM (activity equivalent to normal media).
      
The release of enzyme activity from endothelial cells was inversely proportional to calcium concentrations ranging between 0.01 mM (activity equivalent to calcium-free media) and 0.5 mM (activity equivalent to normal media).
      
Salt tolerance in this culture was characterizedby an altered growth behavior, reduced cell volume, and accumulation of Na+, Cl-, proline and sugars when grown under salt stress, as well as on normal media.
      
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Objective:To dimonstrate the effects of the extracts from Sophora Subprostrata chun et T chan(SSCC) on the proliferation and mitochondria metabolism of human hepatoma SMMC 7721 cells.Methods:After the test of dose dependent experiment,human hepatoma cells were treated with SSCC extracts at dose of 250 μg/ml for 48 h and mitochondria metabolism activity was detected by MTT method.The experiments of effects of SSCC extracts on the proliferation were performed with the treatments of SSCC extracts at dose of 500...

Objective:To dimonstrate the effects of the extracts from Sophora Subprostrata chun et T chan(SSCC) on the proliferation and mitochondria metabolism of human hepatoma SMMC 7721 cells.Methods:After the test of dose dependent experiment,human hepatoma cells were treated with SSCC extracts at dose of 250 μg/ml for 48 h and mitochondria metabolism activity was detected by MTT method.The experiments of effects of SSCC extracts on the proliferation were performed with the treatments of SSCC extracts at dose of 500 μg/ml for 7 d and the proliferation of human hepatoma cells were also examined after treated with SSCC extracts for 24 h and then replaced by cultured in normal media(containing 10% FCS) for 72 h.Result:(1)SSCC extracts could significantly decrease the activity of mitochondria metabolism,and the A570 absorption in the treatment group was only 62.1% of that in control group after 48 h treatment and 9.64% after 72 h treatment.(2)The human hepatoma cell number in the treatment group was much lower than that in control groups during the two groups were treated for seven days.(3)Had been treated for 24 h then cultured in normal media for 72 h,the human hepatoma cells slowly increased in number;(4)After treated for 24 h,the mitosis index in the treatment group was only 50.6% of that in the control group.Conclusion:SSCC extracts could effectively inhibit the proliferation and mitochondria metabolism activty of human hepatoma cells. [

目的:研究中药山豆根(SSCC)提取物对体外培养的人肝癌细胞的抑癌活性。方法:SSCC经粉碎、浸提、超速离心、减压浓缩、冷冻干燥得SSCC提取物。人肝癌SMMC-7721细胞经SSCC提取物处理不同时间后,以MTT法测SSCC提取物对人肝癌细胞线粒体代谢活性的影响,以细胞记数法观察了其对细胞增殖的影响。结果:1经SSCC提取物处理48h,其A570光吸收降为对照组的62.1%,72h为9.64%;2SSCC提取物处理7d后,人肝癌细胞数明显低于对照组;3经SSCC提取物处理24h后,在更换为正常培养基恢复培养过程中,细胞数呈现缓慢增长;4经SSCC提取物处理24h后其有丝分裂指数为对照组的50.6%。结论:中药SSCC提取物能抑制体外培养的人肝癌细胞的增殖,降低线粒体代谢活性。

AIM To demonstrate the effects of the extracts from traditional Chinese medicine Bolbostemma paniculatum (Maxim) Franquet (BPF) on the proliferation and mitochondria metabolism of human hepatoma SMMC-7721 cells.METHODS The dried BPF was ground to powder, extracted in cold distilled water and centrifuged to discard the pellet and the BPF supernatant was concentrated and lyophilized. After the dose-dependent experiments, human hepatoma cells were treated with 200mg/L BPF extract for 48 h and mitochondria metabolism...

AIM To demonstrate the effects of the extracts from traditional Chinese medicine Bolbostemma paniculatum (Maxim) Franquet (BPF) on the proliferation and mitochondria metabolism of human hepatoma SMMC-7721 cells.METHODS The dried BPF was ground to powder, extracted in cold distilled water and centrifuged to discard the pellet and the BPF supernatant was concentrated and lyophilized. After the dose-dependent experiments, human hepatoma cells were treated with 200mg/L BPF extract for 48 h and mitochondria metabolism activity was detected by MTT method.RESULTS BPF extracts could significantly decrease the activity of mitochondria metabolism, the A570 absorption in BPF treatment group was only 31.3% of that in control group after 48 h treatment and 21.4% after 72 h treatment; after 24 h treatment with BPF, the cell numbers in experimental group were 0.57,0.316,0.29,0.285,0.57, 1.287 and 1.2,in control group were 1.82, 3.67, 6.2, 9.4, 10.1 and 11, 9 and in the NON group were 1.7, 3.35, 5.6, 8.4, 8.9, 9.3 and 7.6. The numbers were not only lower than that in control group but also than that before treatment and maintained lower than that before treatment in the whole treatment (7d) (P<0.01); the cell numbers did not significantly increase after the cells were treated for 24 h and then replaced by culture in normal media for 96 h.CONCLUSION BPF extracts could inhibit the proliferation and mitochondria metabolism activity of human hepatoma cells.

目的 研究中药土贝母(BPF)水提物对体外培养的人肝癌细胞的抑癌活性。 方法 BPF经粉碎、浸提、超速离心、减压浓缩、冷冻干燥得BPF水提物,人肝癌SMMC-7721细胞经BPF提取物处理不同时间后以MTT法测其对人肝癌细胞线粒体代谢活性的影响,以细胞记数法观察了其对细胞增殖的影响。 结果 ①经200mg/L BPF提取物处理48h,人肝癌SMMC-7721细胞线粒体活性为对照组的31.3%,处理72h为21.4%;②以100mg/L BPF提取物处理7d中,人肝癌细胞数分别为0.57,0.316,0.29,0.285,0.57,1.287,1.2,对照FCS组分别为1.82,3.67,6.2,9.4,10.1,11,9,NON组分别为1.7,3.35,5.6,8.4,8.9,9.3,7.6,实验组人肝癌细胞数不仅低于对照组,而且低于处理前细胞数(P<0.01);③经BPF提取物处理24h后,即使更换为正常培养基,人肝癌细胞数在96h的恢复培养中基本无增加。 结论 中药BPF提取物能显著抑制体外培养的肝癌细胞增殖和降低线粒体代谢活性。

Objective To demostrate the effects of the extracts from traditional Chinese drug Dryopteris cras-sirhizoma Nakai (DCN) on the proliferation and mitochondria metabolism of human hepatoma SMMC~7721 cells. Methods The dried DCN was broken to powder, extracted in cold DW and centrifuged to discard the pellet and the DCN supernatant was concentrated and lyophilized. Human hepatoma cells were treated with 50ug/ml DCN extracts for 48h and mitochondria metabolism activity was detected by MTT. After treated with DCN...

Objective To demostrate the effects of the extracts from traditional Chinese drug Dryopteris cras-sirhizoma Nakai (DCN) on the proliferation and mitochondria metabolism of human hepatoma SMMC~7721 cells. Methods The dried DCN was broken to powder, extracted in cold DW and centrifuged to discard the pellet and the DCN supernatant was concentrated and lyophilized. Human hepatoma cells were treated with 50ug/ml DCN extracts for 48h and mitochondria metabolism activity was detected by MTT. After treated with DCN extracts for 24h and then replaced by cultured in normal media (containing 10% PCS) for 96h. Results DCN extracts could were treated for 24h and then replaced by cultured in normal media for 96h, the cells entered immedeately into logarithmic phase after the drug was removed from the medium. Conclusion DCN extracts could inhibit the proliferation and mitochondria metabolism activity of human hepatoma cells.

目的研究中药贯众(DCN)提取物对体外培养的人肝癌细胞的抑癌活性。方法 DCN经粉碎、浸提、超速离心、减压浓缩、冷冻干燥得DCN提取物。人肝癌SMMC-7721细胞经DCN提取物处理不同时间后以MTT法测其对人肝癌细胞线粒体代谢活性的影响,以细胞计数法观察了其对细胞增殖的影响。结果(1)DCN提取物能明显抑制人肝癌细胞的生长,降低线粒体代谢活性;(2)经DCN提取物处理24h后的人肝癌细胞,既使更换为正常培养基,其线粒体代谢活性及细胞增殖与对照相比仍差异显著。结论中药DCN提取物含抑癌活性物质,能抑制体外培养的肝癌细胞增殖和降低线粒体代谢活性。

 
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