The expressions of p16~ Ink4a and Rb proteins were also related to the pathological grades. The positive rate of p16~ Ink4a was significantly higher in G_2 and G_3 than that in G_1(P<0.05), and that of Rb was significantly lower in G_3 than in G_1 (P<0.05).
To provide the highest penetration of the laser emission into a medium, the irradiation of samples were carried out with tuning far from the maximum of the Si-O bond absorption band (1055 cm-1) using the 10P16 line (Ωlas= 947.78 cm-1).
In addition to the known nuclear reaction 14N(n, γ)15N, which allows the nitrogen component of explosives to be detected, it is proposed to use the reaction 16O(n, p)16N (β-, γ) → 16O.
Multiplex methylation-sensitive PCR was employed in studying the methylation of the RB1 and CDKN2A/p16 promoter regions in 52 retinoblastomas.
Methylation of p16 was for the first time observed in retinoblastoma (9 tumors, 17%).
Aberrant methylation of the p16 promoter was the second mutation event in two tumors and was not accompanied by RB1 defects in one tumor.