METHODS The drug resistance and mecA gene of the β-lactamase and aac(6′)/aph(2″),aph(3′)-Ⅲ,ant(4′,4″) genes of aminoglycoside and qac(A/B) disinfectant-resistant genes were(detected) in 47 strains of MRSA.
Aminoglycoside antibiotic M 41 A was isolated from the fermentation broth of a strain of Micromonospora olivoasterospora M 41. Based on its physico chemical properties and spectral evidences, andtibiotic M 41 A has been identified as verdamicin.
Compared with K-B,the category agreements of Phoenix-100 for gentamicin for screening high level aminoglycoside resistant(HLAR),ampicillin,ciprofloxacin,erythromycin,nitrofurantoin,vancomycin,and teicoplanin were 94.0%,94.0%,88.0%,94.0%,70.8%,64.0% and 92.0% respectively;
Objective To investigate drug resistance and genotyping of aminoglycoside modifying enzymes(AMEs) in 21 strains of Pseudomonas aeruginosa isolated from the clinical samples.
WUYIMEISU (FORTIMICINS), AN AMINOGLYCOSIDE ANTIBIOTIC PRODUCED BY MICROMONOSPORA ——Ⅲ. ISOLATION AND PURIFICATION OF WUYIMEISU A & B, PHYSICO-CHEMICAL PROPERTIES AND ANTIBACTERIAL ACTIVITY
The positive rate of aminoglycosides modifying enzyme genes aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰand ant(2″)-Ⅰexpression was 8.11%(3/37),43.24%(16/37),18.92%(7/37) and 40.54%(15/37) respectively.
the detection rate of 6 aminoglycoside-modifying enzyme genes, including ant(2”)-Ⅰ, aac(3)-Ⅱ, aac(6’)-Ⅱ, aac(6’)-Ⅰ, ant(3”)-Ⅰ and aac(3)-Ⅰ was 78.6%, 57.1%, 57.1%, 7.1%, 14.3% and 0.0% respectively;
METHODS Drug-reisistant genes encoding AMEs such as aac(3)-Ⅰ,aac(3)-Ⅱ,aac(6′)-Ⅰ,aac(6′)-Ⅱ,ant(3″)-Ⅰ and ant(2″)-Ⅰ were detected by polymerase chain reaction(PCR)(amplification) in 21 PAE isolates.
In a weak acidic medium, the reaction of some aminoglycoside antibiotics (AGs) such as kanamycin sulfate (KANA), gentamycin sulfate (GEN), and tobramycin sulfate (TOB) with acid thiazolyl bisazo dye Titan Yellow (TY) can result in the fading of TY.
This method has high sensitivity and can be applied to the determination of commercial aminoglycoside antibiotics and serum samples with satisfactory results.
A new version of enzyme immunoassay with the use of an amperometric immunosensor was proposed for the determination of an aminoglycoside antibiotic gentamicin.
Sisomicin, an aminoglycoside antibiotic, was used as sole bactericidal therapy in sixteen cases of severe bronchopulmonary infection for an average of 11.5 days (range 7 to 14) with a dose of 3.5 mg/kg per day in three intramuscular injections.
Use of combination therapy consisting of an anti-Pseudomonas penicillin and an aminoglycoside has been shown to offer the greatest success in the treatment ofPseudomonas pulmonary infections.
Using the Inhibition Zone Measurement Instrument Model Z-82-A, we could accurately measure the amount of pure antibiotics and the multi-component of the antibiotics as well as the ratio of the individual factor of new antibiotics in thin layer chromato-graphy with bioautcgraphlc detection. In thin layer chrcmatography of kanamycin, neomy-cin, and tobramycin with silica gel GF 254 plastic film and silica gel sintered glass plate, satisfactory results were obtained. In the case of multi-component amino glycos...
Two hundred seventy nine of the 292 patients with acute pyogenic infections were given with medemycin (China-made Medicamycin) intravenously at a daily dosage of 0.8 to 1.5 million units for 5 to 10 days. The clinical responses were satisfactory in 276(88.8%) patients. Thirteen patients suffering from severe systemic infe-ctions were treated in conjunction with other antibiotics (most of them with aminoglycosides) , and the responses were satisfactory too.Medemycin has remarkable potency against gram-positi...
A strain of streptomyces. 83-1050B was isolated from a soil sample collected from Jil Xiang prefecture in Sichuan Province. According to its morphological,cultural, biochemical characteristics, antimicrobial activity and the chemical composition of cell wall, the strainwas very conformable with the Nebramycins producer-Str. tenebrarius described in thelitera ture, yet there are still some differences between them. We considered, it is a new strain of Str. tenebrarius and give the name Str. tenebrarius 83-10...