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peripheral monocytes
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  外周血单核细胞
     Changes in the expression of β_2-integrin on the peripheral monocytes during perioperative period
     围术期外周血单核细胞β_2整合素表达的变化
短句来源
     Results: 1.The VCAM-1 expression by peripheral leukocytes: (1)The VCAM-1 expression by peripheral monocytes and lymphocytes before delivery was significantly different between PIH group and normal group (P<0.001, 0.01);
     结果:外周血单核细胞、淋巴细胞VCAM-1的表达:(1)产前外周血单核细胞、淋巴细胞VCAM-1的平均对数荧光强度指数,妊高征组与对照组比较,差异均有极显著性(P<0.001,0.01)。
短句来源
     (Results:) The expression level of GM-CSFR in peripheral monocytes of LN patients was significantly higher than that of normal subjects(P<0.01).
     结果:LN外周血单核细胞GM-CSFR表达水平显著高于正常对照组(P<0.01);
短句来源
     RhoA/ROK signal pathway is required for LPS-induced cytokine secretion by human peripheral monocytes
     RhoA/ROK信号通路对LPS诱导的人外周血单核细胞分泌细胞因子的调控作用
短句来源
     The expression of HLA-DR on peripheral monocytes was measured in 54 patients by flow cytometry on 24h、 3d、5d、7d、10d and 14d of imtensive care in parallel with serum CRP , severity of the disease (APACHE II score, SOFA score) and clinical data collection.
     在入院的24h、3d、5d和7d、10d、14d用流式细胞仪测定外周血单核细胞HLA-DR的变化,并同时收集炎症反应(血TNF-a、IL-6和C-RP)、病情严重度(APACHE Ⅱ评分、SOFA评分)、感染并发症和预后等资料,在两组之间进行比较。
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  “peripheral monocytes”译为未确定词的双语例句
     Results The BTK expressions in peripheral monocytes of the patient and his mother were 96.9% and 97.8% respectively.
     结果患者及其母亲细胞内BTK表达分别为96.9%和97.8%。
短句来源
     Compared with the conventional treatment, tight control of blood glucose also increased the HLA-DR expression of peripheral monocytes, and there were significantly difference on 3d,5d and 7d (p<0.05).
     严格控制血糖组HLA-DR表达的恢复较常规治疗组更快,第3、第5和第7天存在显著差异(p<0.05);
     Compared with the conventional treatment, tight control of blood glucose also increased the HLA DR expression of peripheral monocytes, and there were significantly difference on 3 d, 5 d and 7 d ( P <0 05).
     严格控制血糖组HLA DR表达的恢复较常规治疗组更快 ,入院后 3d、5和 7d存在显著差异 (P <0 0 5 ) ;
短句来源
     THYMOSIN α1 AND INTERFERON-α IN REGULATING C-C CHEMOKINES IN NEONATE CORD AND ADULT PERIPHERAL MONOCYTES
     胸腺肽α1和α干扰素对新生儿和成人单核细胞分泌三种C-C趋化因子的调节作用
短句来源
     Immunologic status (distribution of T-cell subgroups and IL-2 activity induced by peripheral monocytes) was investigated in 13 cases of childhood aplastic anemia.
     本文对13例小儿再障的细胞免疫状况及T淋巴细胞亚群和外周血淋巴细胞诱生的白细胞介素-2(IL-2)活力进行测定。 其中3例作免疫治疗前后的动态比较。
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  相似匹配句对
     Methods Human peripheral blood monocytes were isolated.
     方法 分离人外周血单个核细胞;
短句来源
     THE OBSERVATION ON THE ENZYMATIC ACTIVITY IN HUMAN PERIPHERAL BLOOD MONOCYTES
     人周围血单核细胞酶活性之观察
短句来源
     PERIPHERAL PSUDOANEURYSMS
     周围动脉假性动脉瘤
短句来源
     (2) peripheral;
     (2)周边强化型;
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  peripheral monocytes
In contrast, in peripheral monocytes HIV-1 DNA was not regularly found.
      
Varying numbers of FACS-sorted CD4+ cells, CD8+ cells and peripheral monocytes were assayed for HIV-1 proviral DNA (env andgag region) by PCR.
      
Following each viremic episode, provirus levels in the peripheral monocytes decreased to less than 1 copy in 5×106 cells.
      
The secretion of IL-6 from peripheral monocytes was significantly higher in glioma patients compared to controls (P = 0.0003).
      
In this study,peripheral monocytes and lamina propria mononuclearcells (LPMNC) were isolated from IBD patients andnormal controls.
      
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We studied the total cell-associated A14-125I-insulin radioactivity (TBd, including surface-bound and internalized radioactivity), insulin internalization (In), and the effects of lysosomotropic agent Chloroquine (CQ) on postreceptor processing in peripheral monocytes (Mo) from the patients with non-insulin-dependent diabetes mellitus (NIDDM, n=6) and normal controls (n= 7). The results indicate that 1) insulin is able to bind to and internalize into Mo; 2) the abilities of TBd and In of Mo from the patients...

We studied the total cell-associated A14-125I-insulin radioactivity (TBd, including surface-bound and internalized radioactivity), insulin internalization (In), and the effects of lysosomotropic agent Chloroquine (CQ) on postreceptor processing in peripheral monocytes (Mo) from the patients with non-insulin-dependent diabetes mellitus (NIDDM, n=6) and normal controls (n= 7). The results indicate that 1) insulin is able to bind to and internalize into Mo; 2) the abilities of TBd and In of Mo from the patients with NIDDM are significantly decreased (4.03±0.40 vs 9.40±0.87 and 1.95±0.44 vs 6.06±0.63 pg/107Mo); 3) intracellular insulin degradation is reduced in Mo from the diabetics; and 4) lysosomotropic agent CQ, has no effects on intracellular A14-125I-insulin radioactivity in Mo from both diabetic and normal subjects. These defects may be related to the cellular insulin resistance present in patients with NIDDM.

本研究测定了NIDDM病人(n=6)和正常人(n=7)循环血中单核细胞(Mo)结合~(125)I-胰岛素总能力、胰岛素-受体复合物进入能力、胰岛素降解能力和趋溶酶体制剂氯喹对受体后过程的影响。结果显示NIDDM时Mo对胰岛素的结合能力、进入能力和降解能力均明显低于正常人。这些改变可能与NIDDM胰岛素抵抗性有关。

Immunologic status (distribution of T-cell subgroups and IL-2 activity induced by peripheral monocytes) was investigated in 13 cases of childhood aplastic anemia. In the diseased children, readings of CD3 and CD4 were higher with CD8 lower than those in the controls, resulting in a significantly lowered CD4/CD8 ratio (0.91 ± 0.46 VS 1. 67 ± 0.24, p<0.01). The mean level of IL-2 activity was significantly increased (529. 76 ± 36.84u/mlVS 316.31 ± 53.99 u/ml, p<0.01). These immunologic disturbances were...

Immunologic status (distribution of T-cell subgroups and IL-2 activity induced by peripheral monocytes) was investigated in 13 cases of childhood aplastic anemia. In the diseased children, readings of CD3 and CD4 were higher with CD8 lower than those in the controls, resulting in a significantly lowered CD4/CD8 ratio (0.91 ± 0.46 VS 1. 67 ± 0.24, p<0.01). The mean level of IL-2 activity was significantly increased (529. 76 ± 36.84u/mlVS 316.31 ± 53.99 u/ml, p<0.01). These immunologic disturbances were corrected in all the 4 patients receiving immuno-suppressive therapy (2 cases with ATG and 2 cases with CSA) with satisfactory remissions.

本文对13例小儿再障的细胞免疫状况及T淋巴细胞亚群和外周血淋巴细胞诱生的白细胞介素-2(IL-2)活力进行测定。其中3例作免疫治疗前后的动态比较。结果显示患儿总T 细胞(CD_3)及T辅助细胞(CD_4)均低于正常儿,而T抑制细胞(CD_8)则高于正常儿,使CD_4/CD_8的比值明显低于正常儿(0.91±0.46:1,正常儿1.67±0.24:1,P<0.01)。而IL-2活性在患儿白细胞中高于正常高限(529.76±±36.84比316.31±53.99,P<0.01),说明患儿有免疫紊乱状态。应用免疫抑制治疗(ATG,环孢素A等),可纠正这种不平衡状态,临床也有良好的效应。

It is well-known that the human granulocyte colony-stimulating factor(hG-CSF) cDNA is extremely difficult to express in E. coli. Previously we cloned the highly active form of hG-CSF cDNA(pBVG4) from stimulated peripheral monocytes. Using pBVG4 as PCR template, the altered one of hG-CSF cDNA which contains the last codon(GCC-Ala) of its signal peptide and the coding region of its entire mature peptide (AAla-hG-CSF) was cloned and located downstream the /I phage PRPL promoter and E. coli atpE translational...

It is well-known that the human granulocyte colony-stimulating factor(hG-CSF) cDNA is extremely difficult to express in E. coli. Previously we cloned the highly active form of hG-CSF cDNA(pBVG4) from stimulated peripheral monocytes. Using pBVG4 as PCR template, the altered one of hG-CSF cDNA which contains the last codon(GCC-Ala) of its signal peptide and the coding region of its entire mature peptide (AAla-hG-CSF) was cloned and located downstream the /I phage PRPL promoter and E. coli atpE translational initiation region(TIR). The candidate recombinants were identified by in situ hybridization with specific probe and induced by temperature-shift(from 37℃ to 42℃ ) to express AAla-hG-CSF in E.coli . The lysates of expressing bacteria showed obvious granulocyte colony-stimulating activity as assayed in vitro. The above results demonstrated that the combination of A phage PRPL promoter and E.coli atpE TIR was effective in initiating expression of hG-CSF cDNA in E.coli.

hG—CSF cDNA在天然状态下很难在大肠杆菌中表达。本文以作者克隆的高活性形式hG—CSF cDNA为模板,经PCR扩增出含hG—CSF信号肽中丙氨酸(Ala)及成熟型全部氨基酸编码序列的cDNA片段,于NcoⅠ、BamHⅠ位点克隆于原核表达载体PJLA602中,用高活性hG—CSF特异探针菌落原位杂交筛选出阳性克隆。此克隆hG—CSF cDNA在λ噬菌体 P_RP_L串联启动子和大肠杆菌atpE翻译起始区(TIR)共同作用下,阳性菌株热诱导表达后经小鼠骨髓细胞体外CFU-G测试表明,表达产物具明显的粒细胞集落刺激活性。

 
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