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whole cdna
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  全长cdna
     Methods:Clone plasmid pUC18/VEGF165 containing the whole cDNA sequence of VEGF165 was combined with pcDNA3.1(+) to reconstruct pcDNA3.1/VEGF165.Sixty-nine AVNFH model New Zealand adult rabbits with a mean weight of 2.8 kg were randomly divided into three groups.
     方法:将含人VEGF165全长cDNA序列的克隆载体pUC18/VEGF165与pcDNA3.1(+)载体构建成重组真核表达质粒pcDNA3.1/VEGF165; 69只AVNFH造模成功后的新西兰大白兔随机分成3组。
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     RT-PCR was applied to amplify the whole cDNA of TRAIL gene.
     采用RT-PCR从人胎盘中扩增出TRAIL基因的全长cDNA
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     Douglas’s Group in UBC, Canada isolated whole cDNA fragment of 4CL genes, Ptd4CL3, from Populus trichocarpa x P. deltoides, whose proteins catalyze the CoA ligation of hydroxycinnamic acids, generating activated phenolic precursors for lignin biosynthesis, while Ptd4CL3 is strongly expressed in stem and is thought to be devoted to lignin biosynthesis in developing xylem tissue.
     加拿大UBC 大学Douglas 小组获得了杨树4CL 酶的编码基因Ptd4CL3 的全长cDNA 片段,该基因与木质素生物合成有关,其重组蛋白具有4CL 酶活性,且在木质部中特异性表达。
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     Aim To clone the whole cDNA of tumor associated gene MAGE 1 in human hepatocellar carcinoma cell line HHCC.
     目的 克隆人肝癌细胞系HHCC中的肿瘤相关基因MAGE 1的全长cDNA
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     Cloning of Whole cDNA Sequence, Expression, Locating and Function Analysis of Stress Inducible Phosphoprotein Ⅰ Gene
     磷酸化应激诱导蛋白基因全长cDNA序列的克隆、表达、定位和功能研究
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  “whole cdna”译为未确定词的双语例句
     Cloning the whole cDNA of human cytochrome 1A1 gene by nest- PCR
     巢式PCR法构建人细胞色素P4501A1基因cDNA全长T载体
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     The whole cDNA of the enzyme consists of 855 bp and the genomic DNA of PEL is composed of 1135 bp and has six exons and five short introns (58 bp, 47 bp, 50 bp, 56 bp, and 69 bp).
     该脂肪酶cDNA全长由855个碱基组成; DNA全长由1135个碱基组成,含有5个内含子,大小分别为58bp、47bp、50bp、56bp和69bp。
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     Objective To construct the pGEM-T vector containing the whole cDNA of human cytochrome 1A1(CYP1A1)gene with two recognition sites for the enzymes KpnI and XhoI and provide experimental,material for subclone and toxicological study.
     目的构建含KpnI、XhoI双酶切位点的人细胞色素(CYP1A1)基因cDNA全长的pGEM-T载体,为以后的亚克隆和毒理学研究提供实验材料。
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     The whole cDNA locus was mapped to mouse chromosome 3A1~A2 by BLAST analysis to the mouse genome.
     mPC 1基因cDNA全长为 2 193bp,主要定位于小鼠染色体 3A1 A2区域.
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     The whole cDNA sequence and genomic DNA sequence were obtained and submitted to GenBank and the accession numbers were AF284064 and AF330635 respectively.
     将扩增产物与载体pBluescript Ⅱ SK(+)连接,通过T7和T3引物测序获得了该脂肪酶的cDNA全序列(GenBank登陆号AF284064)和DNA全序列(GenBank登陆号AF330635)。
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     Result The whole cDNA gene was successfully fished.
     结果成功钓取了IL-6相关EST电子克隆的全长cDNA基因。
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     Sequencing the Whole cDNA of Hepatitis E Virus from Changchun
     一株长春地区戊型肝炎病毒全基因cDNA序列测定
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     The whole cells of R.
     直接将霉菌R.
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     Methods The cDNA of g.
     方法合成g.
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     cDNA Gigapack?
     cDNA Gigapack(?)
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  whole cdna
One hundred maize zygotic embryos microdissected at the transition stage were used to construct a cDNA library after non-selective PCR (NS-PCR) amplification of whole cDNA populations.
      
The Bpx and BPX sequences are highly conserved, with 79.8% identity over the whole cDNA sequence.
      
This difference is probably caused by the probe used in the previous study, which was a whole cDNA, including the MADS domain.
      
Obviously, a whole cDNA expression library can also be tested if required.
      
Five independent PCR clones encoding each region were sequenced to verify that a correct whole cDNA sequence had been cloned.
      


he lysates of 3T3HER2/neu,a 3T3 cell line transfected with whole cDNA of human HER2/neu oncogene and expressing an 185 kDa oncogenic protein of HER2/neu,was used to immunize the female Balb/c mice.The resultes show that:the immunized mice splenocytes have strong specific proliferation response against 3T3HER2/neu cells (mean stimulation index,SI=315±188),but low level proliferation to 3T3 control cells (SI=114±097).The specific CTL to p185 oncoprotein can be elicited from one immunized mouse...

he lysates of 3T3HER2/neu,a 3T3 cell line transfected with whole cDNA of human HER2/neu oncogene and expressing an 185 kDa oncogenic protein of HER2/neu,was used to immunize the female Balb/c mice.The resultes show that:the immunized mice splenocytes have strong specific proliferation response against 3T3HER2/neu cells (mean stimulation index,SI=315±188),but low level proliferation to 3T3 control cells (SI=114±097).The specific CTL to p185 oncoprotein can be elicited from one immunized mouse splenocytes after a culture proceduce in vitro sensitization and high level antip185 antibody (OD=102±016) is detected in the immunized mice sera.There are significant differation between experiment and control group (OD=036±010)(P<0001).The studies suggest the p185 oncogenic protein encoded by HERE2/neu gene,as a tumor antigen,can induced the specific immune response.

用表达p185蛋白HER2/neu转基因3T3-HER2/neu细胞裂解物免疫Balb/c小鼠,经3次免疫后,免疫小鼠脾细胞对表达p185蛋白的3T3-HER2/neu细胞呈现较强的增殖反应(平均刺激指数分别为SI=3.15±1.88),而对未转染HER2/neu基因的3T3细胞的应答较弱(SI=1.14±0.97)。并检测到1号免疫鼠脾细胞对3T3-HER2/neu细胞有特异杀伤作用。免疫鼠血清中出现高水平的抗p185抗体(OD均值=1.02±0.16),较正常对照组(OD均值=0.36±0.10)有显著差异(P<0.001),表明HER2/neu表达产物p185蛋白具有免疫原性,用于免疫小鼠可诱导出对p185蛋白的特异免疫应答反应。

Objective To clone the cDNA of GCF-5 antigen and to study its expression in E. Coli.Methods Cultured GCT cells from surgical specimens were used for total RNA isolation with Guanidine HCLand organic solvents. Oligo(dT) -cellulose was used in selection of poly (A) + RNA. Then, synthesis of cDNA,ligation with synthetic EcoR I adapter, and constructing cDNA libraries with bacteriophage A gt 11 Sfi-Notvectors are carried out with Ribo Clone system. After packaging in vitro, the phages were transferred...

Objective To clone the cDNA of GCF-5 antigen and to study its expression in E. Coli.Methods Cultured GCT cells from surgical specimens were used for total RNA isolation with Guanidine HCLand organic solvents. Oligo(dT) -cellulose was used in selection of poly (A) + RNA. Then, synthesis of cDNA,ligation with synthetic EcoR I adapter, and constructing cDNA libraries with bacteriophage A gt 11 Sfi-Notvectors are carried out with Ribo Clone system. After packaging in vitro, the phages were transferred to adsorbE. Coli. Y1090. Immunoscreening of lambda expression libraries with GCF-5 McAb and further cloning wasrepeated with the same procedure. The phages from positive clone was then adsorhed to E. Coli. Y1089.Transcription initiation was regulated with IPTG at 42℃ Cyto-immunology block assay was performed toidentified the fusion proteins, which contain GCF-5 antigen. Results The concentration of poly(A)+RNApurified by Oligo (dT) -cellulose affinity reached 20. 56 g/ml. The ratio of OD260/280 is 1. 869. Gel analysisof cDNA showed it distributed from hundreds to thousands of bp. In the presence of X-Gal and IPTG, re-combinant phage plaques were colorless. The control was blue. Screening the library with GCF-5 McAb, apositive plaque had been isolated. In subsequent repeated cloning, all plaques were GCF-5 stained. Thepositive phages from the positive plaque were used to adsorb E. Coli. Y1089, and cultured scparately at 32℃and 42℃ . The lysogens, which grew confluently at 32℃ and spottily at 42℃, were cultured and induced toproduce fusion proteins with IPTG at 42℃. SDS-PAGE showed a slightly larger band than -galactosidase in thelysogens. GCF-5 McAb, after co-incubation with the crude lysate of the lysogens, could not stain the GCTcell, where as the GCF-5 McAb, incubated with crude lysate of control phage showed positive stain. Conclu-sions After a series of complex procedure, a whole cDNA library of GCT was constructed. GCF-5 antigen'scDNA was cloned out by GCF-5 McAb. The fusion protein contain the GCF-5 antigen were produced by in-duction with IPTG and proper temperature. Cyto-immunology block assay confirmed all of the result.

目的 GCF-5是骨巨细胞瘤(GCT)特异性单抗,其抗原是了解GCT特性的途径之一。为了利用这一途径,对GCF-5抗原的cDNA进行了克隆及表达。方法 盐酸胍和有机溶剂法提取总RNA,Oligo(dT)-Cellulose亲和层析法分离mRNA,反转录合成cDNA,用 gt11Sfi-Not表达载体构建cDNA文库,免疫学筛选并克隆GCF-5抗原的cDNA,大肠杆菌Y1089表达含GCF-5抗原的融合蛋白,细胞免疫抑制实验证实该融合蛋白含有GCF-5抗原成分。结果 经Oligo(dT)-Cellulose亲和层析法分离的mRNA,浓度达20.56μg/ml,OD260/280比值为1.869,经反转录所得的cDNA产物从数百到数千bp之间,产物完整;构建的 gt11Sfi-Not载体经体外包装后均呈无色透明噬斑,用抗体筛选出了一阳性噬斑,重复克隆化所得噬斑均可被抗体染色;该噬菌体感染大肠杆菌Y1089,分别于32℃与42℃培养,选出溶源菌,IPTG诱导融合蛋白,SDS-PAGE显示稍大于正常β-半乳糖苷酶的融合蛋白带,与融合蛋白预孵育后的抗体所做细胞免疫化学染色不显色。结论 构建了完整的GCTcDNA文库;?

Objective To clone and identify the whole cDNA of MXR7 gene and to fied out its exprmsion in human normal and tumor tissues. Method The DNA primers were designed and syntheised acordingto the whole cDNA sequence of MXR7 gene. The cDNA of human HCC was taken as template while the cDNA of MXR7 gene was synthesized by polymerase chain reaction (PCR), Recombinant DNA conforming toreading frame was constructed by connecting purified PCR product of the cDNA of MXR7 gerre with...

Objective To clone and identify the whole cDNA of MXR7 gene and to fied out its exprmsion in human normal and tumor tissues. Method The DNA primers were designed and syntheised acordingto the whole cDNA sequence of MXR7 gene. The cDNA of human HCC was taken as template while the cDNA of MXR7 gene was synthesized by polymerase chain reaction (PCR), Recombinant DNA conforming toreading frame was constructed by connecting purified PCR product of the cDNA of MXR7 gerre with expression vector pGEX-5X-1 of fusion protein. The plasmid MXR7/pGEX-5X-1 was identified by sequencing. Us ing 32 P labeled MXR7cDNA as probe, MXR7mRNA expression was detected by Northern blot analysis in 12different human normal tissues, 7 preoperatively untreated non-liver tumor tissue, 30 preoperatively UntreatedHCC and the corresponding surrounding non-cancerous hepatic tissues samples, 12 normal liver tissues samplesconfirmed pathologically. Results Enzyme digest and sequence analysis confirmed that the insertion sequencein vector PGEX-5X-1 was the same as the cDNA sequence of MXR7 gne. Northern blot analysis showed nodetectable expression of MXR7 mRNA in 12 human normal tissues including liver, 7 non-HCC tumor tissuesand 12 normal liver tissues and the frequencies of MXR7 mRNA expression in HCC and the coresponding sur rounding non-cancerous hepatic tissues are 76.6% and 13.3%, respectivdy. Concluslon MXR7 mRNAhigh expression in human HCC was common and specific, suggesting MXR7 mRNA may serve as a tumorbiomarker for HCC.

进行MXR7基因cDNA的克隆鉴定并检测其在人正常组织和肿瘤组织中的表达。方法以人肝癌组织的cDNA为模板,应用PCR方法合成MXR7基因cDNA,连接于融合蛋白表达载体pGEX-5X-1,构建成符合读框的融合基因;应用Northernblot对正常人12种不同组织和术前做治疗的7例非肝脏肿瘤组织及30例肝癌和癌旁肝组织、12例正常肝组织中MXR7基因的表达进行检测。结果MXR7mRNA在人肝脏等12种正常组织及7例其它部位肿瘤组织中均不表达;在人肝癌中呈高表达,而癌旁肝组织中呈低表达,其阳性表达率分别为76.7%和13.3%。结论MXR7.基因在人肝癌组织中的高水平表达具有普遍性和特异性,提示MXR7基因可以作为肝癌的生物学标志物。

 
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