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culture primary
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  原代培养
     Conclusion It is an effective method to culture primary RAECs from explants digested for 1h by collagenase type Ⅰ(2.0 g·L-1),that can shorten primary culture time.
     结论经2.0g·L-1Ⅰ型胶原酶消化1h的组织块,组织块迁出率及内皮细胞迁出速度明显提高,从而大大缩短原代培养的时间。
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     AIM:To culture primary retinal microvascular endothelial cells (RMEC) from Wistar rats with magnetic cell sorting (MACS) system and to observe the effects of cocultured Müller cells on proliferation and migration of RMEC.
     目的:用免疫磁珠法原代培养大鼠视网膜微血管内皮细胞(RMEC),观察共培养Müller细胞对视网膜微血管内皮细胞增生和迁移的影响。
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     Modified culture system (serum free, 3T 3 cells free) was used to culture primary human oral keratinocytes with modified tissue culture technique in vitro.
     采用改良的无血清及无 3T3 细胞的培养体系和改良组织块法原代培养口腔黏膜上皮细胞。
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     PURPOSE: To culture primary mouse odontoblast and to provide a base for study on inducing ES cells to odontoblast.
     目的:原代培养小鼠成牙本质细胞,为诱导胚胎干细胞(ES细胞)向成牙本质细胞分化提供基础。
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     Objective To culture primary human umbilical vein endothelial cells in vitro and research its radiation-effect.
     目的 :初步探讨人脐静脉内皮细胞 (HUVEC)原代培养的电离辐射效应。
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  “culture primary”译为未确定词的双语例句
     During 2 weeks of culture, primary cells began to double for 3-4 days and reached confluency at 7-8 days after passage.
     原代细胞在培养2周后可达到融合,一般其倍增时间为3~4d,传代后7~8d即可达到融合。
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     On the Impact of Chinese Traditional Culture on Corporate Culture Primary Hypothesis
     论中国传统文化对组织文化基本假设的影响
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     Conclusion To culture primary porcine keratinocytes with the medium that DKSFM containing 10% FBS and to cultivate the second passage with the medium containing 5% FBS, the proliferation of porcine keratinocytes are faster.
     结论10%胎牛血清的DKSFM培养原代猪角朊细胞,5%胎牛血清的DKSFM培养传代猪角朊细胞,细胞生长速度快。
短句来源
     To investigate the stable model for cultivating hUASMC in vitro. Obtain the human umbilical artery,use the methods of sticking pieces and sticking pieces plus enzymolysis to isolate and culture primary hUASMC in vitro.
     探索人脐动脉平滑肌细胞(human umbilical artery smooth muscle cell,hUASMC)体外培养稳定的模型。
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     The culture philosophy is often comprehendedat three meanings: science of culture, primary theory of culturology andphilosophical cultural philosophy.
     人们通常在三种层面上理解文化哲学:关于文化的科学,关于文化学的元理论,哲学的文化哲学。
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  相似匹配句对
     MethodsrRLECs were primary culture.
     方法:取RLECs进行原代培养。
短句来源
     Primary Culture of Guayule
     银胶菊初代组织培养技术研究
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     CULTURE
     论文化
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     Culture
     文化
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  culture primary
We have utilized a system to culture primary human airway epithelial cells in order to study the interaction of NTHI and human airway epithelium.
      
To date, only a few laboratories worldwide have been able to culture primary human isolates.
      
Keratinocyte culture Primary human epidermal keratinocytes were obtained according to the Rheinwald-Green system.
      


Objective Primary human thymic epithelial cell growth was compared under different culture conditions.The effect of supernatant on T Cell line 85 cell proliferation and on 85 cell srvival were investigated. Methods Culthe ofhuman thymic epithelial cells .Proliferation assay of 85 Cells, Trypan- blue staining and FACS analysis . Results Serum containing medium and growth factor containing medium both could culture primary thymic epithelial cells. TES. alone could promote the proliferation of 85 cells...

Objective Primary human thymic epithelial cell growth was compared under different culture conditions.The effect of supernatant on T Cell line 85 cell proliferation and on 85 cell srvival were investigated. Methods Culthe ofhuman thymic epithelial cells .Proliferation assay of 85 Cells, Trypan- blue staining and FACS analysis . Results Serum containing medium and growth factor containing medium both could culture primary thymic epithelial cells. TES. alone could promote the proliferation of 85 cells and improve the nsurvival rate of 85 cells. Conclusion Ther was no obvious thebetween containing medium and growth factor containing medium. Cutting alone could culture pure thymic epithelialcell. TES could promote the proliferation of 85 Cells. This indicate that there are maybe unknown factors in supernatants. TEScould maitin 85 cell's subsistence. Maybe supernatant affect growth cycle of T cell line.

目的比较不同培养条件下原代人胸腺上皮细胞生长情况,观察上清液对T细胞林85细胞增殖及存活的效应。方法原代胸腺上皮细胞培养、85细胞增殖测定、台盼兰试验及FACS分析。结果合血清的培养基与合生长日子的培养基均能培养出原代胸球上皮细胞,TES上清单独能促使85细胞增殖且能提高85细胞的存活率。结论研究发现合血清的培养基与合生长因子的培养基培养效果无明显差异,仅剪切也能培养纯净的麻腺上皮细胞。TES上清单独能促使85细胞增殖,提示上清液中可能存在一种未知的细胞因子。上清液能维持85细胞生存,可能与影响T细胞生长周期有关。

Objective To study the regulation of prostaglandin E 2 to OPGmRNA expression in osteoblasts in order to elucidate the mechanism of prostaglandin E 2 induced bone resorption.Methods Culture primary osteoblastic cells from calvaria of newborn SD mice,treated with prostaglandin E 2 according to the experimental design.Total cellular ribonucleic acid was isolated and semiquantitative reverse transcriptase PCR method were used to screen the level of OPGmRNA.Result Prostaglandin E 2 dose- and time-dependently...

Objective To study the regulation of prostaglandin E 2 to OPGmRNA expression in osteoblasts in order to elucidate the mechanism of prostaglandin E 2 induced bone resorption.Methods Culture primary osteoblastic cells from calvaria of newborn SD mice,treated with prostaglandin E 2 according to the experimental design.Total cellular ribonucleic acid was isolated and semiquantitative reverse transcriptase PCR method were used to screen the level of OPGmRNA.Result Prostaglandin E 2 dose- and time-dependently down-regulated the mRNA levels of OPG.OPGmRNA levels were significantly decreased.Conclusion The mechanism of inhibiting OPG expression in osteoblasts might be involved in prostaglandin E 2 induced bone resorption.

目的 研究前列腺素E2 (PGE2 )对成骨细胞OPG基因表达的调节 ,探讨PGE2 引发骨吸收的机制。方法 体外培养大鼠成骨细胞 ,分别在不同时间、给予不同剂量PGE2 进行干预试验 ,提取总RNA进行半定量逆转录PCR分析 ,检测OPGmRNA水平的改变。结果 PGE2 可以抑制OPG基因的表达 ,其作用强度随时间、剂量而改变。结论 PGE2 调节骨代谢、增加骨吸收的作用 ,与其下调成骨细胞OPG基因表达 ,诱导破骨细胞形成及功能有关。

Objective To study the regulation of prostagla ndin E1toos teoprotegerin mRNA expression in osteoblasts in order to elucidate the mechanism of the effection of pro staglandin E 1 on BMD.Method Culture primary osteoblastic cells from calvaria of newborn SD mice,treated with prostaglandin E 1 .Cells were collected and semiquant itative reverse transcriptase PCR m ethod was used to screen the mRNA levels of OPG.Result Prostaglandin E 1 increase the expression of OPG mRNA a nd the effect...

Objective To study the regulation of prostagla ndin E1toos teoprotegerin mRNA expression in osteoblasts in order to elucidate the mechanism of the effection of pro staglandin E 1 on BMD.Method Culture primary osteoblastic cells from calvaria of newborn SD mice,treated with prostaglandin E 1 .Cells were collected and semiquant itative reverse transcriptase PCR m ethod was used to screen the mRNA levels of OPG.Result Prostaglandin E 1 increase the expression of OPG mRNA a nd the effect varied with time and dose.Conclusion The regulation of OPG mRNA in osteoblast might be involved in the effection of prostaglan din E 1 increase BMD.Prostaglandin E1inhi bit osteoclast differention by the way of increasing OPG expression.

目的探讨前列腺素E1PGE1的作用机制是否涉及保骨素OPG基因表达的改变从而影响破骨细胞分化。方法体外培养大鼠成骨细胞,分别给予不同时间、剂量的前列腺素E1进行干预实验,提取总RNA进行半定量逆转录聚合酶链反应PCR分析,检测OPGmRNA水平的改变。结果PGE1具有促进OPGmRNA表达的作用,强度随时间和浓度而改变。结论PGE1抑制骨吸收、促进成骨的作用与OPG对信号传导的调节有关。

 
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