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   anti digoxin 的翻译结果: 查询用时:0.171秒
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anti digoxin
相关语句
  地高辛
     anti - BrdU anti - human PDGF - B anti - human PDGF( R) anti - human MMP - 1 anti -human MMP - 9, monoclonal anti - rat pancreatic elastase, goat anti digoxin alkaline phosphatase immunocomplex;
     抗BrdU抗体,抗人PDGF-B抗体,抗人PDGF(R)β抗体,抗人MMP-1、MMP-9抗体,多克隆抗大鼠胰腺弹性蛋白酶抗体,羊抗地高辛-碱性磷酸酶复合物;
短句来源
     Aim: To study the effect of antidigoxin antiserum on oxygen stress induced by myocardial ischemia/reperfusion(MI/R) injury in rats.
     目的:探讨地高辛抗血清对大鼠实验性心肌缺血/再灌注损伤(MI/R)的心肌氧化应激的影响。
短句来源
     Aim To research the changes of myocardial endoxin level in rats with myocardial ischemia reperfusion (MIR) and the protevtive effects of anti digoxin antiserum, an endoxin specific antagonist, on MIR injury.
     目的 结扎大鼠左冠状动脉前降支造成心肌缺血再灌注损伤 (MIR) ,观察MIR时心肌组织内洋地黄素水平的变化和内洋地黄素特异性拮抗剂地高辛抗血清对MIR时心肌的保护作用。
短句来源
     Producing the Anti digoxin Antibody
     抗地高辛抗体的制备
短句来源
     (3) CoxB 3m virus in myocardial tissue was detected using in situ RT PCR by direct incorporated technique which employed nucleotide labeling by anti digoxin antibody and bonded with alkaline phosphatase (anti dig AKP method).
     (3)应用碱性磷酸酶联接的抗地高辛抗体标记的核苷酸直接掺入法建立原位PCR检测心肌组织中柯萨奇B3m病毒RNA。
短句来源
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  地高辛
     anti - BrdU anti - human PDGF - B anti - human PDGF( R) anti - human MMP - 1 anti -human MMP - 9, monoclonal anti - rat pancreatic elastase, goat anti digoxin alkaline phosphatase immunocomplex;
     抗BrdU抗体,抗人PDGF-B抗体,抗人PDGF(R)β抗体,抗人MMP-1、MMP-9抗体,多克隆抗大鼠胰腺弹性蛋白酶抗体,羊抗地高辛-碱性磷酸酶复合物;
短句来源
     Aim: To study the effect of antidigoxin antiserum on oxygen stress induced by myocardial ischemia/reperfusion(MI/R) injury in rats.
     目的:探讨地高辛抗血清对大鼠实验性心肌缺血/再灌注损伤(MI/R)的心肌氧化应激的影响。
短句来源
     Aim To research the changes of myocardial endoxin level in rats with myocardial ischemia reperfusion (MIR) and the protevtive effects of anti digoxin antiserum, an endoxin specific antagonist, on MIR injury.
     目的 结扎大鼠左冠状动脉前降支造成心肌缺血再灌注损伤 (MIR) ,观察MIR时心肌组织内洋地黄素水平的变化和内洋地黄素特异性拮抗剂地高辛抗血清对MIR时心肌的保护作用。
短句来源
     Producing the Anti digoxin Antibody
     抗地高辛抗体的制备
短句来源
     (3) CoxB 3m virus in myocardial tissue was detected using in situ RT PCR by direct incorporated technique which employed nucleotide labeling by anti digoxin antibody and bonded with alkaline phosphatase (anti dig AKP method).
     (3)应用碱性磷酸酶联接的抗地高辛抗体标记的核苷酸直接掺入法建立原位PCR检测心肌组织中柯萨奇B3m病毒RNA。
短句来源
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  “anti digoxin”译为未确定词的双语例句
     Using the T3DIG conjugate and a immobilized polyclonal rabbit antiT3 antibody and a labeled polyclonal rabbit antidigoxin F(ab′)2, a sensitivity of 0.089 ng/ml was obtained for T3. Physiological T4 concentration do not interfere with the T3 assay. 
     此方法测定T3的敏感性为0.089ng/ml,生理浓度的T4对其无干扰作用
短句来源
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  anti digoxin
Fractions 2 and 19 also cross-reacted with anti-digoxin antibodies, and another significant immu noreactive peak was present in fraction 8.
      
Anti-digoxin scFv fragments expressed in bacteria and in insect cells have different antigen binding properties.
      


A study on the interaction among digoxin, specific anti-digoxin monoclonal antibody (MAb) and Na+,K+-ATPase was performed with rabbit red blood cell (RRBC) membrane. In vitro experiment with pooled RRBC membrane preparation showed that the Na+, K+-ATPase activity was highly sensitive to digoxin and the IC50 was 95.4pM in our testing system. This inhibition was effectively antagonized by the specific anti-digoxin MAb FD8 (immunoglobulin subclass IgGl, affinity to digoxin...

A study on the interaction among digoxin, specific anti-digoxin monoclonal antibody (MAb) and Na+,K+-ATPase was performed with rabbit red blood cell (RRBC) membrane. In vitro experiment with pooled RRBC membrane preparation showed that the Na+, K+-ATPase activity was highly sensitive to digoxin and the IC50 was 95.4pM in our testing system. This inhibition was effectively antagonized by the specific anti-digoxin MAb FD8 (immunoglobulin subclass IgGl, affinity to digoxin hapten being 4.05×108M-1). MAb FD8 ascites of 1.8×104 fold dilution could lead to 50% recovery of the Na+,K+-ATPase activity inhibited by digoxin of 4 ng/ml concentration. After transfusion of MAb FD8 ascites to rabbits which had been given digoxin 1 mg/kg intravenously, the advanced inhibition of RRBC membrane Na+,K+-ATPase was soon recoveredand the degree of recovery was related to the dose of MAb given. The increase of intracellular Na+ concentration and decrease of intracellular K+ concentration caused by Na+,K+ -ATPase inhibition were also returned to about normal after venoclysis of MAb FD8. Results of radioimmu-noassay showed that the amount of free digoxin in the plasma of those rabbits was significantly reduced by MAb treatment. This work indicates that specific anti-digoxin MAb is a highly effective antagonist to digoxin and also suggests that the antidigoxin MAb's of high affinity combat with digoxin toxic ity by enhancing dissociation of digoxin -Na+,K+-ATPase complex.

本文研究结果表明抗地高辛特异性MAb可有效地拮抗地高辛引起的RRBC膜Na~+,K~+-ATP酶活性抑制。1.8×10~4倍稀释的FD8 MAb腹水,在试管内可使4ng/ml浓度地高辛引起的酶活性抑制恢复达50%。给地高辛中毒兔iv FD8 MAb 腹水后,被抑制的RRBC膜Na~+,K~+-ATP酶活性迅速恢复,酶抑制造成的RRBC内Na~+,K~+浓度异常也随之得到恢复。本研究从受体水平证实了特异性MAb解除地高辛毒性的作用机理

Digoxin and digitoxin can be detected by single sweep polarography in 0.01M ICH_2COONH_4-0.2M NH_4Cl basal solution. The specific immunochemical reaction of digoxin and digitoxin tomonoclonal anti-digoxin antibody, and the cross reaction of other steoid compounds can be deter-mined homogeneously by detecting the decreased peak currents of these haptens. This is a rapidhomogeneous voltammetric immunoassay for studying the monoclonal anti-digoxin antibody.

在含碘乙酸的氯化铵缓冲液中,地高辛和洋地黄毒甙可用单扫示波极谱法检测。抗地高辛单克隆抗体对地高辛和洋地黄毒甙的特异性免疫化学反应,和与其它甾体化合物的交叉反应,可由同一体系中直接测量此类半抗原峰电流受抑制的程度进行鉴别。建立了研究抗地高辛单克隆抗体特征的快速均相伏安免疫法。

Streptavidin (6 25 μg/ml, sigma)diluted in distilled water was coated for 3 h ( 100 μl/well ) at room temperature to microtiter strips. Anti digoxin antibody was modified by biotin. The assay with these strips was used for the measurement of digoxin. It provideds a sensitivity of 0 0964μg/L. The assay has an intra assay coefficient of variation of 8 9% (at 0 5012 μg/L), 5 9% (at 1 5631 μg/L), 2 4% (at 3 9678 μg/L). The inter assay coefficient of variation is 15 8% (at 0 8722...

Streptavidin (6 25 μg/ml, sigma)diluted in distilled water was coated for 3 h ( 100 μl/well ) at room temperature to microtiter strips. Anti digoxin antibody was modified by biotin. The assay with these strips was used for the measurement of digoxin. It provideds a sensitivity of 0 0964μg/L. The assay has an intra assay coefficient of variation of 8 9% (at 0 5012 μg/L), 5 9% (at 1 5631 μg/L), 2 4% (at 3 9678 μg/L). The inter assay coefficient of variation is 15 8% (at 0 8722 μg/L), 10 1% (at 1 1867 μg/L), 9 2% (at 3 1726 μg/L ). In spiking experiments with serum samples, the mean recovery of added digoxin is 97 45% (range 89 07%~107 22%). The assay was compared with FPIA . A linear regression analysis yielded a slope of 0 9640, an intercept of 0 1564 and a correlation coefficient of 0 9488.

采用国产链霉亲和素直接包被塑料板孔 ,生物素标记抗体 ,建立的竞争酶联免疫吸附试验的方法测定血中地高辛浓度 .其测定灵敏度为 0 0 964μg/L ,最低检测限为 0 2 2 51 μg/L ,测定三份低、中、高浓度的血清标本 ,批内变异系数为 8 9% ,5 9% ,2 4 % ;批间变异系数为 1 5 8% ,1 0 1 % ,9 2 % ,测定回收率在 89 1 %~ 1 0 7 2 2 %之间 ,此法与FPIA方法相关良好 (r=0 94 88)

 
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