Results The NCSE scores in cognitive intervention group after treatment were 44.64±7.82which were higher than 36.26±7.73before treatment and 38.73±7.61in control group after treatment (t=3.56,14.32,P<0.01).
④The expressions of protein and mRNA of caspase 3 in every group: The levels of protein and mRNA of caspase 3 in combination group were obviously lower than those in the simple MPP+ group (t =0.05-0.32,P < 0.05).
After 24h incubation, cellular DNA was isolated and analyzed for BP-derived DNA adducts by 32P-postlabeling technique.
A binary system of photoaffinity reagents was proposed earlier for highly efficient labeling of DNA polymerases by 5"-[32P]DNA primers.
A photoactive 2,3,5,6-tetrafluoro-4-azidobenzoyl (FAB) group was incorporated at the 3"-end of 5"-[32P]DNA primers synthesized by DNA polymerase β or Tte in the presence of one of the dTTP analogs-FAB-4-dUTP, FAB-9-dUTP, or FAB-4-ddUTP.
The interaction of surface proteins from A431 cells and cellular extracts with nucleic acids was investigated using affinity modification with 32P-labeled reactive oligonucleotide derivatives.
Proteins with affinity for nucleic acids were also revealed in the Ruk1 Glu-tagged preparation by retardation of electrophoretic mobility of 32P-labeled oligodeoxyribonucleotides in gel.