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Three series (L-7,L-8 and L-9) of Koch bodies of Theileria annu- lata which had been passaged in tissue cultures for 6,18,35 and 42/45 generations respectively were used in the experiment.11 cattle divided into five batches were inoculated s.c or Ⅳ.with one of the series.At the same time,9 Cattle were used as controls.All except two of the vaccinated animals resisted safely when challeged subsequently by tick biting. The interesting thing is that some of the vaccinated animals remai- ned very resistant.even... Three series (L-7,L-8 and L-9) of Koch bodies of Theileria annu- lata which had been passaged in tissue cultures for 6,18,35 and 42/45 generations respectively were used in the experiment.11 cattle divided into five batches were inoculated s.c or Ⅳ.with one of the series.At the same time,9 Cattle were used as controls.All except two of the vaccinated animals resisted safely when challeged subsequently by tick biting. The interesting thing is that some of the vaccinated animals remai- ned very resistant.even though no blood type and typical koch bodies of Theileria annulata were present.The immunological mechanism is bri- efly discussed. 本文报导了体外培养的牛环形泰氏焦虫考赫氏体不同培养代数的牛只反应性和抗蜱叮咬再感染的免疫原性。试验分五批进行。使用的免疫接种物分别为 L—7系18代、L—7系35代、L—7系42/45代(二次免疫)、L—8系、L—9系6代、L—8系18代和L—9系18代混合、L—8系和 L—9系19代混合材料。免疫接种一个月后以蜱叮咬再感染,观察免疫牛的免疫性。结果表明:1.牛环形泰氏焦虫考赫氏体经体外组织培养继代,毒力迅速减弱。2.接种第6代和18代培养物的牛获得了坚强的免疫力。接种 L—7系35代和42/45代培养物的牛,再感染时发病较重。部分动物死于泰氏焦虫病。由此推断,对牛安全性良好、免疫原性坚强的最适宜的制苗代数应在10~20代之间。 This study discribed 50 cases of recurrent pneumonia (RP) with at least 3 attacks annually. All of them were investigated immunologically and were followed up by our special clinic. It was found that 36% of these patients were immunocompromised, including primary immunodeficiency diseases (5 cases) and secondary or temporary lowering of immunity (13 cases). In the rest of patients, there were 16 cases having allergic histories and/or signs, it appeared that another 32% of RP cases also have been involved with... This study discribed 50 cases of recurrent pneumonia (RP) with at least 3 attacks annually. All of them were investigated immunologically and were followed up by our special clinic. It was found that 36% of these patients were immunocompromised, including primary immunodeficiency diseases (5 cases) and secondary or temporary lowering of immunity (13 cases). In the rest of patients, there were 16 cases having allergic histories and/or signs, it appeared that another 32% of RP cases also have been involved with immunological mechanism. Analysis of the immunological and clinical data showed that the onset of RP usually occurred before 6 month of age in the patients with primary-combined immunodeficiency diseases or other congenital mulformations, after 4-5 years of age in patient with concomitant antibody deficiency and developed under 3 years of age in the group without routinely detectable immunodeficiency. The clinical signs of the atopic patients with pneumonia may be hyper-expressed while the pneumonia happened in immunocompromised patients may be hypo-expressed clinically. To correct or improve the lowered immune function seems to be important for the treatment of RP. Anti-histamine agents may have to be given in the very beginning of respiratory infection in order to prevent the patients from RP. 本文报道在1年内发生3次或3次以上的反复肺炎患儿50例。分析他们的免疫水平与临床表现,发现伴有免疫功能低下或缺陷者占36%,可能有过敏因素参与者占32%,提示了免疫因素在小儿反复的下呼吸道感染发病机理中具有重要意义。对这些病人的临床特点、诊断依据与免疫治疗提出探讨与建议。 Normal pregnancy is a successful semi-hemeotransplantation, but spontaneous abortion is a failure of this kind of hemeotransplantation. Some researches demonstrated that high HLA histocompatibility between abortion patients and their husbands was an important factor of spontaneous abortion. Mixed lymphocyte culture (MLC) is a transplantation reaction activated by allotypic antigen in vitro, which is similar to the situation of immunity of pregnancy. Therefore, MLC can serve as an effectve method in the studies... Normal pregnancy is a successful semi-hemeotransplantation, but spontaneous abortion is a failure of this kind of hemeotransplantation. Some researches demonstrated that high HLA histocompatibility between abortion patients and their husbands was an important factor of spontaneous abortion. Mixed lymphocyte culture (MLC) is a transplantation reaction activated by allotypic antigen in vitro, which is similar to the situation of immunity of pregnancy. Therefore, MLC can serve as an effectve method in the studies of the immunological mechanisms of spontaneous abortion. In the studies of prevention and treatment for spontaneous abortion with Chinese medicine, the kinetic changes of one-way MLCs between pregnant women, who once experienced abortion, and their husbands can be observed before and after the treatment, so that the action of immunoregulation of Chinese medicine exerted on the patients can also be studied. 正常妊娠是一种成功的半同种异体移植,流产则是这种异体移植的失败.研究表明:配隅间HLA抗原的高度相容性是造成流产的重要原因.混合淋巴细胞培养(MLC)是一种同种异型抗原激发产生的体外移植免疫反应,所以较接近妊娠免疫,因此,MLC可作为自然流产的免疫机理研究的一个较有效的方法.在中医防治自然流产的研究中,我们可以观察中药治疗前后的孕期流产妇女与其丈夫间的单向MLC的动态变化,从而探讨中药治疗对患者机体的免疫反应的调节作用.
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