助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   p 16 pro 的翻译结果: 查询用时:0.009秒
图标索引 在分类学科中查询
所有学科
更多类别查询

图标索引 历史查询
 

p pro
相关语句
  p16蛋白
     ResultsAfter t reatment with 8-MOP/UVA, fibroblasts displayed morphological and biological cha nges of cell senescence with a permanent switch of mitotic to stably postmitotic phentypes as well as increasing expression of SA-β-galactosidase and p16 pro tein.
     结果  8 MOP/UVA作用后 ,培养真皮成纤维细胞迅速出现细胞衰老特征性的形态学及生物学特性改变 :细胞由分裂表型转化为无分裂活性表型 ,SA β Gal表达增加、p16蛋白表达增加。
短句来源
     Results: The P 16 pro te in expression rate in adenoid cystic carcinoma tissue were 33.33%.
     结果 :36例腺样囊性癌中 ,P16 蛋白阳性表达 12例 ( 33 3% ) ;
短句来源
     19 cases with protein unexpression had geneovariation MTS1/p16 pro tein deletion was positively correlated with pathological stages and clinical pe riods in endometrial carcinoma, gene deletion and 5CpG island methylation indu ced p16 protein inactivation.
     9例发生基因缺失,12例发生甲基化,未发现基因缺失与甲基化同时存在的病例,p16基因总失活率58.33%; 22例p16蛋白表达缺失标本有19例基因失活。
短句来源
  相似匹配句对
     (16) D.
     ⒃马毛虱D.
短句来源
     * (16!)
     *(16!)
短句来源
     Measurement of P16-.
     10.绒癌继发于正常妊娠组p16、P15基因联合阴性率42.9%; 继发于葡萄胎组0%;
短句来源
     On day 16 p.
     p.
短句来源
     ,Pro.
     、脯氨酸(Pro.)
短句来源
查询“p 16 pro”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
没有找到相关例句


Objective: To observe the serum concentration differences of pro-collagen Ⅲ (PP) Pro-collagen Ⅳ(PP) and hyaluronic acid(HA) and study the effects of intervention on Pro-collagen formation and inhibition of left ventricular hypertrophy (LVH) in essential hypertensives(EH) before and after Enalapril treatment. Methods Serum concentrations of PP, PP and HA were measured by radioitnmunoassay (RIA ) in 37 EH patients before and after taking Enalapril...

Objective: To observe the serum concentration differences of pro-collagen Ⅲ (PP) Pro-collagen Ⅳ(PP) and hyaluronic acid(HA) and study the effects of intervention on Pro-collagen formation and inhibition of left ventricular hypertrophy (LVH) in essential hypertensives(EH) before and after Enalapril treatment. Methods Serum concentrations of PP, PP and HA were measured by radioitnmunoassay (RIA ) in 37 EH patients before and after taking Enalapril orally for 12 weeks along with 21 healthy individuals as control group. Results Serum concentrations of PP, PP and HA were significantly higher than those of the control group (P<0.01). The relative indices (PP, PIVP, HA) in EH patients after treatment were markedly lower than those before treatment. Conclusion Serum concentrations of PP, PIVP and HA are closely related to the rise of blood pressure. Enalapril reduces the formation of the serum concentrations of PP, PP and HA when it effectively controls blood pressure and reverses left ventricular hypertrophy.

目的 观察原发性高血压(EH)患者使用依那普利前后血清Ⅲ、Ⅳ型前胶原、透明质酸的浓度变化;探讨依那普利干预血清前胶原生成以及抑制左室肥厚(LVH)形成的作用。方法应用放免技术测定37例EH患者及21例体检正常者血清Ⅲ、Ⅳ型前胶原神经末端肽(PⅢP、PⅣP)及透明质酸(HA)浓度。EH患者口服依那普利治疗12周后复测上述指标。结果治疗前EH组血清PⅢP、PⅣP、HA浓度显著高于对照组(P<0.01);依那普利治疗后EH组相应指标较治疗前明显降低(P<0.01)。结论血清PⅢP、PⅣP、HA水平与血压升高密切相关;依那普利在降压的同时,降低血清PⅢP、PⅣP、HA的生成,具有抑制LVH的作用。

Objective To investigate the fused expression of secreted protein Ag85B-ESAT6 of Mycobacterium tuberculosis , and to provide a promising preventive subunit vaccine against tuberculosis. Methods The gene encoding Ag85B and ESAT6 protein was amplified by PCR from genome of Mycobacterium tuberculosis H_ 37Rv strain,and inserted into cloning vector P GEM-T-easy. After sequence analysis, and digestion by restriction endonuclease, Ag85B-ESAT6 was cloned into corresponding sites of the expression vector P...

Objective To investigate the fused expression of secreted protein Ag85B-ESAT6 of Mycobacterium tuberculosis , and to provide a promising preventive subunit vaccine against tuberculosis. Methods The gene encoding Ag85B and ESAT6 protein was amplified by PCR from genome of Mycobacterium tuberculosis H_ 37Rv strain,and inserted into cloning vector P GEM-T-easy. After sequence analysis, and digestion by restriction endonuclease, Ag85B-ESAT6 was cloned into corresponding sites of the expression vector P PRO EXHT, and the recombinant plasmid was transformed into expressive strain E.coli DH5α,induced with IPTG and fusion protein was purified by Ni-NTA purification system.The specific antibody titer in the sera of BALB/c mouse immunized with two fusion protein was detected by ELISA. Results The sequences of Ag85B and ESAT6 by PCR amplification were identical to those reported by GenBank.The recombinant plasmid fused expression protein of Ag85B-ESAT6 with relative molecular mass(Mr)of 37 000,which was confirmed by Western-blot analysis with specific monoclonal antibody against 6×HismAb. The fused expression protein was insoluble. It could be purified by Ni-NTA purification system.The specific antibody titer in the sera of BALB/c mouse immunized with fusion Ag85B-ESAT6 was 1∶1 000 and that of mouse immunized with fusion protein ESAT6-Ag85B was 1∶5 000. Conclusions Secreted protein Ag85B-ESAT6 of Mycobacterium tuberculosis was successfully fused expressed in E.Coli DH5α. It may become a new type of vaccine against tuberculosis.

目的 融合表达结核分枝杆菌分泌蛋白Ag85B ESAT6 ,为结核病的预防提供有效亚单位疫苗。方法 设计含不同酶切位点的Ag85B和ESAT6引物 ,采用聚合酶链反应 (polymerasechainreaction ,PCR)的方法从结核分枝杆菌毒株H3 7Rv DNA中分别扩增出相应大小的DNA片段 ,将片段分别与PGEM T easy载体连接测序。将测序正确的Ag85B和ESAT6按照不同的酶切位点克隆入PPROEXHT表达载体 ,挑选出阳性克隆 ,将诱导的表达产物进行聚丙烯酰胺凝胶电泳 (SDS PAGE)分析 ,同时与含6个组氨酸 (histidine 6×his)的单克隆抗体 (monoclonalantibody ,mAb)进行固定化蛋白质免疫学测定(Western blot) ,将用含Ni+鳌合剂的Ni NTA亲合柱纯化的表达产物免疫小鼠 ,用结核分枝杆菌培养上清滤液蛋白 (culturefiltrateproteins ,CFP)作为抗原 ,酶联免疫吸附试验 (enzyme linkedimmunosorbentassay ,ELISA)测定免疫小鼠血清特异性抗体的滴度。结果 PCR获得的A...

目的 融合表达结核分枝杆菌分泌蛋白Ag85B ESAT6 ,为结核病的预防提供有效亚单位疫苗。方法 设计含不同酶切位点的Ag85B和ESAT6引物 ,采用聚合酶链反应 (polymerasechainreaction ,PCR)的方法从结核分枝杆菌毒株H3 7Rv DNA中分别扩增出相应大小的DNA片段 ,将片段分别与PGEM T easy载体连接测序。将测序正确的Ag85B和ESAT6按照不同的酶切位点克隆入PPROEXHT表达载体 ,挑选出阳性克隆 ,将诱导的表达产物进行聚丙烯酰胺凝胶电泳 (SDS PAGE)分析 ,同时与含6个组氨酸 (histidine 6×his)的单克隆抗体 (monoclonalantibody ,mAb)进行固定化蛋白质免疫学测定(Western blot) ,将用含Ni+鳌合剂的Ni NTA亲合柱纯化的表达产物免疫小鼠 ,用结核分枝杆菌培养上清滤液蛋白 (culturefiltrateproteins ,CFP)作为抗原 ,酶联免疫吸附试验 (enzyme linkedimmunosorbentassay ,ELISA)测定免疫小鼠血清特异性抗体的滴度。结果 PCR获得的Ag85B和ESAT6序列与基因文库 (GenBank)报道的完全一致。两者在大肠杆菌DH5α株中融合表达的产物约为 370 0 0 ,与预计大小相吻合。Western blot结果显示 ,在相对分子量约 370 0 0处有表达产物与 6×hismAb特异性结合带。表达产物为包涵体 ,通过Ni NTA纯化系统 ,可得到纯化的目的蛋白。Ag8

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关p 16 pro的内容
在知识搜索中查有关p 16 pro的内容
在数字搜索中查有关p 16 pro的内容
在概念知识元中查有关p 16 pro的内容
在学术趋势中查有关p 16 pro的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社