Results The size, infiltration and enhancement pattern on CT scans significantly correlated with p21 WAF1, p16 INK4 and PCNA (P<0.05), while pseudo-capsule formation carried no correlation with p21 WAF1, p16 INK4 and PCNA (P>0.05).
Methods Immunohistochemistry was employed to detect the expression of p16 protein in 114 paraffin embedded primary lung cancer tissues. Loss of p16 INK 4 gene exon 2 (E2) was analyzed using PCR technique in 62 cancer samples with p16 protein negative stain and 14 paracancerous lung tissues.
High risk of lung cancer was established for carriers of rare alleles of the Hras1 minisatellite, the hypermethylated p16INK4A promoter, and microsatellite defects in chromosome regions 3p12, 3p14.2, 3p22-24, 3p21, 3p25, 9p21, and 17p13.
Mutation and abnormal expression of P16INK4a in hepatocellular carcinoma
Objective: To investigate the relationship between p16INK4a and primary hepatocellular carcinoma (HCC), especially hepatitis B-related HCC.
Methods: p16INK4a and its protein in HCC were analyzed with PCR-SSCP and the immunohistochemistry methods respectively.
Results: The positive incidence of p16INK4 protein expressing in HCC was lower than that of normal liver tissue (P>amp;lt;0.05), and the absence of p16INK4 protein was associated with HCC metastasis (P>amp;lt;0.05).