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translation and expression
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  翻译表达
     Objective To verify whether inhibition of the overexpressed eukaryotic initiation factor-4E (eIF-4E) in human colon adenocarcinoma cell line LS-174T may facilitate the degradation of heparanase mRNA and alter the translation and expression levels of heparanase protein.
     目的确定阻抑大肠腺癌LS-174T细胞中过量表达的真核细胞起始因子-4E(eIF-4E)是否促进乙酰肝素酶mRNA的降解,并改变其翻译表达水平。
短句来源
     Objective To study the possibility of translation and expression of mRNA from recombinant yeast in mammalian cells.
     目的 研究酵母mRNA在哺乳动物细胞中的翻译表达
短句来源
  “translation and expression”译为未确定词的双语例句
     Conclusion Defective translation and expression of CTLA-4 take place in patients with IDC, it is possibly related to the CTLA-4 gene microsatellite polymorphism.
     结论IDC患者CTLA-4基因转录和表达缺陷,该缺陷与CTLA-4基因3′末端非翻译区(AT)n重复序列多态性存在关联。
短句来源
     (5) There was no definite result of Northern blotting because of the selection of the probe. But we can inferred by the results from morphology and cytology analysis that the translation and expression of the genes that control the cell division and elongation were likely to be inhibited, and then the mitotic of the wheat root-tip meristematic cell and the growth and development of its primary root were influencd.
     (5) 由于探针选取的问题,Northern杂交未能得到确切结果,但根据形态学和细胞学研究结果推测,DNA甲基化水平的升高很可能抑制了控制细胞分裂和生长的基因的转录和表达,进而影响了小麦根尖细胞有丝分裂和初生根的生长发育。
短句来源
     Besides the architecture of the optimized chain models can also help to understand the procedures of gene translation and expression in eukaryotic cells.
     同时 ,经过优化的模型也有助于了解真核细胞中基因转录和表达的过程。
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  相似匹配句对
     translation.
     三是翻译。
短句来源
     On pictographic translation
     浅议拟形性形译词
短句来源
     On Onomatopoeia And Its Translation
     浅谈拟声词及其翻译对策
短句来源
     On Translation and Culture
     论翻译与文化
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     The expression
     COX-2、VEGF-C mRNA的表达与肿瘤TNM
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  translation and expression
Sequence analysis, in vitro translation and expression of the cDNA for rat liver minoxidil sulfotransferase.
      


Objective To verify whether inhibition of the overexpressed eukaryotic initiation factor-4E (eIF-4E) in human colon adenocarcinoma cell line LS-174T may facilitate the degradation of heparanase mRNA and alter the translation and expression levels of heparanase protein. Methods A 20-mer antisense s-oligodeoxynucleotide (asODN) targeted against the translation start site of eIF-4E mRNA was introduced into LS-174T cells by means of lipid-mediated DNA-transfection, followed ...

Objective To verify whether inhibition of the overexpressed eukaryotic initiation factor-4E (eIF-4E) in human colon adenocarcinoma cell line LS-174T may facilitate the degradation of heparanase mRNA and alter the translation and expression levels of heparanase protein. Methods A 20-mer antisense s-oligodeoxynucleotide (asODN) targeted against the translation start site of eIF-4E mRNA was introduced into LS-174T cells by means of lipid-mediated DNA-transfection, followed by Western blotting analysis and reverse transcription-PCR to determine eIF-4E protein and mRNA levels, respectively. Northern methods was applied to determine heparanase mRNA expression level, with the alterations of heparanase expression assessed by Western blotting analysis. Results The 20-mer asODN against eIF-4E specifically and significantly inhibited eIF-4E protein expression, and as a result, a significant reduction in heparanase mRNA level was observed by Northern blotting in conjunction with significantly decreased heparanase protein expression. Conclusion The inhibition of eIF-4E strongly reduces the stability of heparanase mRNA in colon adenocarcinoma cell line LS-174T and results in an apparent reduction in the expression of heparanase protein.

目的确定阻抑大肠腺癌LS-174T细胞中过量表达的真核细胞起始因子-4E(eIF-4E)是否促进乙酰肝素酶mRNA的降解,并改变其翻译表达水平。方法应用脂质体包裹与eIF-4E mRNA翻译起始点互补的反义寡聚核苷酸,转染处理人大肠腺癌细胞LS-174T。Western blotting和RT-PCR方法分别检测eIF-4E被阻抑后转录和翻译水平的改变。采用Northern blotting定量检测乙酰肝素酶mRNA的细胞内水平,Western blotting检测其蛋白表达水平的改变。结果反义寡聚核苷酸经脂质体转染LS-174T细胞后,eIF-4E基因表达明显受到抑制,其蛋白表达产物也显著下降。伴随eIF-4E被阻抑表达,Northern blotting结果显示乙酰肝素酶mRNA水平下降,且其蛋白翻译表达量也降低。结论阻抑eIF-4E影响LS-174T细胞乙酰肝素酶mRNA的稳定表达,促使其降解,并降低乙酰肝素酶表达。

Objective Splice junction sites are the boundaries between exons and introns in eukaryotic gene sequences. If the splice junction sites in a gene sequence could be predict correctly, the coding regions in a gene would be separated from the non-coding regions. Method This paper proposes a machine learning approach for the modeling and prediction of splice junction sites by an effective feature selection algorithm. This algorithm selects every pair of parent and child node in original chain models for splice...

Objective Splice junction sites are the boundaries between exons and introns in eukaryotic gene sequences. If the splice junction sites in a gene sequence could be predict correctly, the coding regions in a gene would be separated from the non-coding regions. Method This paper proposes a machine learning approach for the modeling and prediction of splice junction sites by an effective feature selection algorithm. This algorithm selects every pair of parent and child node in original chain models for splice junction sites as features, and then uses genetic algorithm and a MAP(Maximum A Posteriori) classifier to select the features. Results and Conclusion The experiment result shows that the new algorithm can optimize the chain models and improve the prediction accuracy of splice junction sites. Besides the architecture of the optimized chain models can also help to understand the procedures of gene translation and expression in eukaryotic cells.

目的 剪接位点是真核细胞生物基因序列中外显子和内含子的相邻区域 ,如果能准确预测基因序列中的剪接位点 ,就能将基因中的表达区域和非表达区域分开。方法 从机器学习的角度出发 ,提出了一种有效的特征选择算法用于剪接位点的建模和预测。该算法首先将初始链模型中每一对父子节点作为特征量提取 ,然后通过遗传算法和最大后验分类器进行特征选择。结果及结论 对剪接位点数据的预测结果显示 ,这种新算法能够有效地优化链模型的结构 ,提高对剪接位点的预测能力。同时 ,经过优化的模型也有助于了解真核细胞中基因转录和表达的过程。

Objective To study the possibility of translation and expression of mRNA from recombinant yeast in mammalian cells. Methods The expression of GFP gene in recombinant yeast was induced by galacose. The mRNA was extracted and identified by RT-PCR and was transfected with liposome into mammalian cell HUVEC and COS-7. Results Green fluorescence appeared in transfected cells and mRNA of GFP gene was translated and expressed in HUVEC and COS-7 cells. Conclusion mRNA of recombinant yeast may be...

Objective To study the possibility of translation and expression of mRNA from recombinant yeast in mammalian cells. Methods The expression of GFP gene in recombinant yeast was induced by galacose. The mRNA was extracted and identified by RT-PCR and was transfected with liposome into mammalian cell HUVEC and COS-7. Results Green fluorescence appeared in transfected cells and mRNA of GFP gene was translated and expressed in HUVEC and COS-7 cells. Conclusion mRNA of recombinant yeast may be used as novel gene therapy materials for oral gene therapy.

目的 研究酵母mRNA在哺乳动物细胞中的翻译表达。方法 半乳糖诱导GFP基因在酿酒酵母细胞中表达,提取其mRNA ,RT -PCR鉴定,并以脂质体为介导,对血管内皮细胞HUVEC以及绿猴肾细胞COS - 7进行体外转染。结果 被转染HUVEC和COS - 7细胞分别出现绿色荧光,报告基因GFPmRNA在动物细胞中被识别、翻译。结论 提示酵母mRNA可能作为基因治疗物质应用于口服基因治疗的研究。

 
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