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binding abilities
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  结合能力
     The competition binding abilities of La2+,Cu2+,Zn2+,Cd2+ to CaM are higher than that of Al3+ and Mg2+.
     结果表明:La3+、Cu2+、Zn2+、Cd2+与钙调素的竞争结合能力较强,Al3+和Mg2+的竞争能力较弱。
短句来源
     The competition binding abilities of Tb~(3+) and La~(3+) to CaM are higher than that of Ca~(2+), and the affinity of Ca~(2+) to CaM is higher than that of Al~(3+).
     结果表明 :Tb3+ ,La3+ 与钙调素的竞争结合能力强于Ca2 + ,而Tb3+的竞争结合力又大于La3+ ,Ca2 +与钙调素的结合力远大于Al3+。
短句来源
     ② To study the effect of PARP1 binding with rTEF1a on their specific DNA binding abilities and the influence of poly(ADP)-ribosylation on the DNA binding ability of PARP1-rTEF1a complex, Southern-Slot ( protein-DNA ) blot method was used.
     使用狭缝DNA 蛋白杂交技术研究PARP1与rTEF1a结合后对它们各自特异性DNA结合能力的影响 ,以及多聚ADP核糖化反应对PARP1 rTEF1a复合物DNA结合能力的影响。
短句来源
     Abjective:To study the influence of combination of poly(ADP-ribose) polymerase I (PARP1) with rTEF1a on their specific DNA binding abilities.
     目的 :研究多聚ADP核糖化酶 1(PARP1)与rTEF1a间的相互作用对各自特异性DNA结合能力的影响。
短句来源
     Antagonistic peptides(APs) AP 1, AP 2 and AP 6 were labelled with biotin at their N terminals. The binding abilities of APs to HHCC or SMMC7721 cells were determined by FACS and confocal microscope.
     分别在HAb18G/CD14 7拮抗肽AP 1、AP 2和AP 6的N端标记生物素 ,用流式细胞仪和激光共聚焦显微镜测定AP 1、AP 2和AP 6与HHCC或SMMC772 1的结合能力
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  “binding abilities”译为未确定词的双语例句
     NF-κB DNA binding abilities activated by TNF-α were abolished by NF-κB decoy ODN, instead of scrambled ODN. TNF-α-induced gene expression of ET-1, iNOS, ICAM-1, VCAM-1 and MCP-1 was significantly inhibited by NF-κB decoy ODN (all P < 0. 01) .
     转染NF-κB decoy ODN可抑制TNF-α激活的NF-κB活性,从而进一步抑制ET-1、iNOS、ICAM-1、VCAM-I和MCP-1的mRNA表达。
短句来源
     HSP90 binding abilities with substrate protein HSP70, Raf-1 were observed by HSP90 coimmunoprecipitation.
     通过免疫共沉淀方法,观察HSP90表达与底物蛋白HSP70、Raf-1的结合情况。
短句来源
     Results After treatment of MCF 7 cells with 4-16 μmol/L LOV for 48-72 h, the expression levels of Rac2 mRNA and RhoGDI mRNA were up regulated, but the binding abilities of Rho GTPases and GTP decreased AT 72 h after treatment.
     结果  4~ 16μmol L的LOV处理MCF 7细胞 48~ 72h后 ,RhoAmRNA表达均无明显变化 ,而Rac2mRNA和RhoGDImRNA随洛伐他汀的处理 ,各剂量组mRNA表达均呈增加趋势 ;
短句来源
     The influence of combination of poly(ADP-ribose) polymerase I with rTEF1a on their specific DNA binding abilities
     多聚ADP核糖化酶与rTEF1a间的相互作用及其影响
短句来源
     The results show that the fluorescent receptor has a high selectivity and fluorescence responsibility to AcO~-. (It's) binding abilities to several anions follow the order: AcO~->p-NO_2C_6H_4OPO~(2-)_3,H_2PO~-_4Cl~-.
     结果表明,该阴离子受体对AcO-具有较高的选择性和荧光响应,对不同阴离子的络合选择性次序为:AcO->p NO2C6H4OPO2-4 Cl-. 3,H2PO-
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  相似匹配句对
     protein binding;
     蛋白结合;
短句来源
     Synthesis of Isonucleoside-Incorporated Oligonucleotides and Their Binding Abilities with Complementary Sequences
     异核苷掺入寡核苷酸的合成及其与互补序列的结合研究(英文)
短句来源
     So we can presume that PAC-L had the abilities of recognizing and binding to the
     脂质体的加入能够明显降低缺氧对心肌细胞的损伤,PAC-L对缺氧心肌细胞的保护作用明显优于Plain-L。
短句来源
     Experiments Exercises Abilities
     实验 习题 能力
短句来源
     abilities for creative teachers;
     创新型地理教师所具备的教育教学观念和教育能力的创新;
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  binding abilities
The extensive variation in lectin sensitivities and lectin-binding abilities between the EC cell lines is diagnostic of the expression of different carbohydrate structures at their respective cell surfaces.
      
The lectin-binding abilities of different EC cell lines also vary and do not necessarily correlate with their relative lectin sensitivities.
      
In the present work, the insulin binding abilities of these molecules have been thoroughly tested.
      
However, the in vitro binding abilities of the MARs to the nuclear matrices isolated by different methods did not change.
      
The binding abilities of maize LTP, presented in this paper, are discussed and compared to those of lipid-binding proteins from animal tissues.
      
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A simple and time-saving method for the purification of ricin using Se-pharose 4B is reported in this paper. We have discovered that there was great difference between the binding abilities of ricin and its related lectin,ricinus agglutinin to Sepharose 4B at pH5.0. In the presence of 0.2 mol/L D-galactose ricin could be eluted from a Sepharose 4B while the agglutinin was sticked on the column, thereby obtaining pure ricin in one-step chromatography.It is not required to synthesize a special affinity-chromatographic...

A simple and time-saving method for the purification of ricin using Se-pharose 4B is reported in this paper. We have discovered that there was great difference between the binding abilities of ricin and its related lectin,ricinus agglutinin to Sepharose 4B at pH5.0. In the presence of 0.2 mol/L D-galactose ricin could be eluted from a Sepharose 4B while the agglutinin was sticked on the column, thereby obtaining pure ricin in one-step chromatography.It is not required to synthesize a special affinity-chromatographic matrix in this method which is particularly useful for the large-scale isolation of ricin.

本文报道了一种单用琼脂糖(Sepharose4B)来纯化蓖麻毒蛋白的快速简便的方法。我们发现在pH5的条件下,蓖麻毒蛋白和与其密切相关的蓖麻凝集素对琼脂糖的结合能力有很大的差别。在有0.2mol/LD-半乳糖存在下可将蓖麻毒蛋白从Sepharose上洗下,同样条件下蓖麻凝集素仍牢固地结合在柱上。从而经一步柱层析便可得到电泳纯的蓖麻毒蛋白。此法不需另行合成亲和胶,适合于蓖麻毒蛋白的大规模纯化。

The enzymological characterizations of site-mutagenized rat recombinant DNA polymeraseβ, RQ182 and RQ183 were studied The phosphocellulose column chromatographies showed that the mutant and the wild DNA polymerases were all eluted by about 0.5 mol/ L KCI, but the denatured DNA-cellulose chromatographies showed that although the wild enzyme was eluted by 0.35 mol/L KCI, RQ182 and RQ183 were eluted by 0.55 and 0.45 mol/L KCI, respectively, indicating that the binding abilities to DNA of the mutant enzymes...

The enzymological characterizations of site-mutagenized rat recombinant DNA polymeraseβ, RQ182 and RQ183 were studied The phosphocellulose column chromatographies showed that the mutant and the wild DNA polymerases were all eluted by about 0.5 mol/ L KCI, but the denatured DNA-cellulose chromatographies showed that although the wild enzyme was eluted by 0.35 mol/L KCI, RQ182 and RQ183 were eluted by 0.55 and 0.45 mol/L KCI, respectively, indicating that the binding abilities to DNA of the mutant enzymes were increased. Km values for the substrate (dTTP)of the wild enzyme, RQ182 and RQ183 were determined as 38.5, 34.5 and 111.1 μmol/L, respectively,and the Km values for the primer (oligo(dT)) were 1.28, 1.96 and 6.58 μg/ml, respectively. The results showed that the affinities of RQ183 to the substrate and the primer were decreased dramatically. It is suggested that Arg182 and Arg183 were involved in the active site function of DNA polymerase β, in binding to DNA template, in recognizing of primer, and in binding to and catalyzing of substrates of the enzyme.

作者对定位诱变克隆的大鼠DNA聚合酶β(pol β)基因产物的酶学特性作了初步研究。结果表明Gln代替Arg~(182)(RQ 182)或Arg~(183)(RQ 183)后,变异的酶对单链模板DNA的结合增强。RQ 182对底物dTTP和引物Oligo(dT)的Km值与野生型pol β相似,但RQ 183对底物的Km值较野生型pol β增加了3倍,对引物的Km值也显著增加,表明RQ 183对底物和引物的亲和力明显下降。推测Arg~(182)和Arg~(183)参与pol β的活性中心功能,并与酶和模板DNA结合,对引物的识别以及与底物的结合和催化有关。

The binding rate saturation curve and Scatchard plot of the reaction between 125I-CKL and the normal/infertility sperms have been studied. The results show that the normal cells have the higher and wider optimun pH range than the infertility ones. Kd value and saturation quantity of the receptors of CKL on normal and infertility sperms are different, which are 41. 67μg/ mL and 2. 5μg/mL, 1. 3×106/cell and 7. 7× 106/cell,respectively. The concentration of the sperms has different effect on the binding abilities...

The binding rate saturation curve and Scatchard plot of the reaction between 125I-CKL and the normal/infertility sperms have been studied. The results show that the normal cells have the higher and wider optimun pH range than the infertility ones. Kd value and saturation quantity of the receptors of CKL on normal and infertility sperms are different, which are 41. 67μg/ mL and 2. 5μg/mL, 1. 3×106/cell and 7. 7× 106/cell,respectively. The concentration of the sperms has different effect on the binding abilities to the 126I-CKL. Monosacchride inhibitions give irregular competitive curve. The results show that the structure of the receptors are complicated, the chemistry properties of normal and infertility sperms are sharply different.

应用(125)I-CKL对正常人和不育患者精子膜上凝集素受体进行研究的结果表明,不育精子膜上凝集素受体的差异,可能是不育的原因之一。

 
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