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spring brassica napus
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  春性甘蓝型油菜
     The results of experiments on sucrose concentration with the spring Brassica napus showed that the number of microspores,survived after 3 days culture in the medium added with 16% sucrose were the largest,as high as 16.13%.
     用春性甘蓝型油菜为材料进行蔗糖浓度的实验结果表明:培养3天后,在16%蔗糖培养基中存活的小孢子最多,达16.13%;
短句来源
     One doubled haploid(DH) population derived from F1 plants of the cross between DH401(P1) and Q2(P2) was used to study the inheritance of flowering time and photoperiod sensitivity of spring Brassica napus in this study. Two parents had exhibited a low and high sensitivity to photoperiod,respectively.
     以对光周期分别表现稳定弱敏感和强敏感的春性甘蓝型油菜品种DH401(P1)与Q2(P2)配制F1代并经小孢子培养获得的DH群体来研究开花期和光周期敏感性的遗传规律。
短句来源
     A genetic linkage map composed of simple sequence repeat(SSR) marker,sequence-related amplified polymorphism(SRAP) marker and amplified fragment length polymorphism(AFLP) marker was built using one DH(doubled haploid) population derived from an F1 between two spring Brassica napus cultivars,early-flowering DH401 and late-flowering Q2,by microspore culture. QTL analysis of flowering time was performed in this DH population.
     在由两个春性甘蓝型油菜双低品种DH401(早花)和Q2(迟花)的F1植株通过小孢子培养所获得的DH(doubled haploid)群体中,应用SSR、SRAP及AFLP标记构建了遗传连锁图谱,并对开花期性状进行了数量性状座位(QTL)分析。
短句来源
  “spring brassica napus”译为未确定词的双语例句
     Breeding of Hufeng 010, a three lines hybrid of spring Brassica napus with double-low
     甘蓝型双低春油菜三系杂交种互丰010的选育
短句来源
     Study on Breeding of Double low Hybrid Kenyou 3 in spring Brassica napus L.
     甘兰型双低春油菜三系杂交种垦油3号的选育
短句来源
     Breeding of Kenyou 5 in Spring Brassica napus L.and Its Cultivation Technique
     甘兰型春油菜垦油5号的选育及简要栽培技术
短句来源
  相似匹配句对
     Spring.
     Spring)水提物中的化学成分。
短句来源
     Spring
     春
短句来源
     Appraisals of Ecotypes of Cultivars in Spring Rape(Brassica campestris L. )
     白菜型春油菜品种生态型鉴定
短句来源
     OBSERVATION ON EMBRYO DEVELOPMENT IN SPRING RAPE(Brassica napus L.)
     甘蓝型春油菜胚胎发育进程的观察
短句来源
     Breeding of Kenyou 5 in Spring Brassica napus L.and Its Cultivation Technique
     甘兰型春油菜垦油5号的选育及简要栽培技术
短句来源
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  spring brassica napus
Doubled haploid (DH) lines were produced from an F1 between two spring Brassica napus cultivars Hyola 401 and Q2.
      


The isolated microspores were cultured by using four winter varieties of Brassica napus growing under the uncontrolled conditions of temperature and light as donor. It was found that the period between third to seventh days was the best time for taking explant, while explants,taken beyond this period,gave no embryoids.In the microspore culture of Brasstica napus only those late-uninucleate microspores could grow into embryoids but in the anther culture,the early-uninucleated microspores tended...

The isolated microspores were cultured by using four winter varieties of Brassica napus growing under the uncontrolled conditions of temperature and light as donor. It was found that the period between third to seventh days was the best time for taking explant, while explants,taken beyond this period,gave no embryoids.In the microspore culture of Brasstica napus only those late-uninucleate microspores could grow into embryoids but in the anther culture,the early-uninucleated microspores tended to grow into embryoids.When the late-uninucleate microspores,developing synchronous were taken in the suitable flowering period,part of them started to enlarge after several hours of culture,which was their earlist marker of development into embryoids.Part of enlarged microspores,then formed multicellular masses and developed further into embryoids. The results of experiments on sucrose concentration with the spring Brassica napus showed that the number of microspores,survived after 3 days culture in the medium added with 16% sucrose were the largest,as high as 16.13%. But the embryoid induction frequency after 30 days culture was highest in the medium with 13% sucrose up to 144 embryoids for one flower bud.When the microspores were cultured in the medium with 16% sucrose for 3 days,and then added an equal volume of medium with 13% sucrose,the embryoid induction frequency was enhanced greatly,up to 3.7 times of that cultured all time in the medium with 13% sugar.The experiment system developed by us for rape,is being used for induction and screening of sclerotinia sclerotiorum resistant mutant and introduction of foreign gene into rape.

以生长在非控温控光下的4个冬性甘蓝型油菜(BrassicanapusL.)品种为供体,进行游离小孢子培养。研究发现,多数品种在开花3-7天取材最为适宜。在甘蓝型油菜小孢子培养中只有单核晚期的小孢子才可能发育成胚状体,而花药培养时处于单核早期的小孢子易于发育成胚状体。在适当花期选取发育比较一致的单核晚期小孢子培养,经数小时后,部分小孢子便开始膨大,这是小孢子发育成胚的最早标志,膨大的小孢子中,有部分形成多细胞球并进一步发育成胚。用春性甘蓝型油菜为材料进行蔗糖浓度的实验结果表明:培养3天后,在16%蔗糖培养基中存活的小孢子最多,达16.13%;培养30天后,胚状体诱导频率则以13%蔗糖浓度为最高,每花蕾可达144个胚状体。如果在16%蔗糖培养基中培养3天后,添加等体积的13%蔗糖培养基,能够大大提高胚状体的诱导频率,为仅用13%蔗糖培养基培养的3.7倍。这一实验体系正在用于抗菌核病的诱变与筛选,并作为外源基因导入的实验体系。

One doubled haploid(DH) population derived from F1 plants of the cross between DH401(P1) and Q2(P2) was used to study the inheritance of flowering time and photoperiod sensitivity of spring Brassica napus in this study.Two parents had exhibited a low and high sensitivity to photoperiod,respectively.Days to flower(DTF) were investigated in the P1,P2 and DH population at Hezheng(Gansu Province) with a long day in summer and at both Zhaoqing(Guangdong Province) and Wuhan(Hubei Province) with a short day in...

One doubled haploid(DH) population derived from F1 plants of the cross between DH401(P1) and Q2(P2) was used to study the inheritance of flowering time and photoperiod sensitivity of spring Brassica napus in this study.Two parents had exhibited a low and high sensitivity to photoperiod,respectively.Days to flower(DTF) were investigated in the P1,P2 and DH population at Hezheng(Gansu Province) with a long day in summer and at both Zhaoqing(Guangdong Province) and Wuhan(Hubei Province) with a short day in winter for consecutive two years.Photoperiod sensitivity index(PSI) was calculated according to DTF obtained from Hezheng and Zhaoqing.The means of DTF and PSI were analyzed by applying a mixed major gene and polygene inheritance model.The results showed that DTF at Hezheng,Wuhan and Zhaoqing was controlled by 3,2,and 2 pairs of major genes with additive and epistatic effect plus polygenes,duplicate effect plus polygene,respectively.Heritability values of the major genes at the three locations were 91.13%,63.05% and 62.02%,and those of polygenes were 4.43%,1.58%,and 22.71%,respectively.The PSI was conditioned by 2 pairs of major genes with epistatic recessiveness effect and some polygenes.The heritability values of the major genes and polygenes were 50% and 37.5%,respectively.Other genetic parameters were also estimated.Therefore,it is speculated that both flowering time and photoperiod sensitivity in this DH population were controlled by 2 or over 2 pairs of major genes and polygenes,and the plants might have some different interaction models among flowering genes under different photoperiods.Meanwhile,how to use the results for Brassica napus breeding was discussed.

以对光周期分别表现稳定弱敏感和强敏感的春性甘蓝型油菜品种DH401(P1)与Q2(P2)配制F1代并经小孢子培养获得的DH群体来研究开花期和光周期敏感性的遗传规律。将P1、P2和DH群体连续2年分别种植在长日照的甘肃省和政县和短日照的广东省肇庆市、湖北省武汉市3地并调查开花期,以从和政和肇庆获得的开花期来计算光周期敏感指数(PSI),采用主基因-多基因混合遗传模型对该DH群体的开花期及光周期敏感指数进行遗传分析。结果表明,在和政、武汉和肇庆,开花期分别由3对加性-上位性主基因+多基因、2对加性-上位性主基因+多基因和2对累加作用主基因+多基因的控制;主基因遗传率分别为91.13%、63.05%和62.02%;多基因遗传率分别为4.43%、1.58%和22.71%。光周期敏感指数由2对隐性上位主基因及多基因控制,主基因和多基因遗传率分别为50%和37.5%。同时也估算了该DH群体的其他遗传参数。因此,开花期和光周期敏感性主要由2对或2对以上主基因及多基因控制,不同光周期条件下开花期基因之间的互作模式有所不同。同时也讨论了本研究结果在育种中的意义。

A genetic linkage map composed of simple sequence repeat(SSR) marker,sequence-related amplified polymorphism(SRAP) marker and amplified fragment length polymorphism(AFLP) marker was built using one DH(doubled haploid) population derived from an F1 between two spring Brassica napus cultivars,early-flowering DH401 and late-flowering Q2,by microspore culture.QTL analysis of flowering time was performed in this DH population.A total of 263 markers including 88 SSR marker loci,101 SRAP marker loci and 74 AFLP...

A genetic linkage map composed of simple sequence repeat(SSR) marker,sequence-related amplified polymorphism(SRAP) marker and amplified fragment length polymorphism(AFLP) marker was built using one DH(doubled haploid) population derived from an F1 between two spring Brassica napus cultivars,early-flowering DH401 and late-flowering Q2,by microspore culture.QTL analysis of flowering time was performed in this DH population.A total of 263 markers including 88 SSR marker loci,101 SRAP marker loci and 74 AFLP marker loci were detected.Out of them,248 markers were assembled into 19 linkage groups (N1~N19).The remaining 15 markers,could not be assembled to any linkage groups.The total map length was 1 634.7cM with an average distance of 6.6cM and a 27.4% of distortion segregation(p<0.01) of markers mainly clustering in N4 and N5.Two and four major-effect QTLs controlling flowering time were identified,which explained 68.63% and 75.83% of phenotype variation in Wuhan and Hezheng,respectively.Moreover,two major-effect QTLs were simultaneously detected in the two locations.In addition epistatic interactions conditioning flowering time were also analyzed.The implications of the results in this study were discussed.

在由两个春性甘蓝型油菜双低品种DH401(早花)和Q2(迟花)的F1植株通过小孢子培养所获得的DH(doubled haploid)群体中,应用SSR、SRAP及AFLP标记构建了遗传连锁图谱,并对开花期性状进行了数量性状座位(QTL)分析。在亲本间共检测到263个有多态性的遗传标记,其中SSR标记有88个、SRAP标记101个及AFLP标记74个。其中248个标记分布于19个连锁群(N1-N19),总遗传距离为1 634.7cM,标记间平均遗传距离为6.6cM,标记偏分离比例达到27.4%(p<0.01)且主要集中在第4、5连锁群。应用QTLMAPPER 1.6在武汉、和政分别检测到2个和4个控制开花期的主效QTL位点,分别解释了68.63%和75.83%的开花期表型变异,其中有2个主效QTL位点在这两地同时被检测到。另外也分析了影响开花期的上位效应并探讨了本研究结果在实际育种中的意义。

 
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