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mitochondria dna encoded genes
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     The six mitochondrial DNA coding genes, ND 4L, ND 4, COX 2, Lys tRNA, ATP 8 and ATP 6 were identified from 69 SSH positive clones. The results indicated that NO can distinctly induce overexpression of mitochondria DNA encoded genes in the esophageal carcinoma cells.
     然后对过表达基因的表达序列标签 (expressedsequencetag ,EST)实施序列测定 ,并与GenBank进行BLAST同源性比较和序列突变分析 . 结果先后两次从 6 9个SSH阳性克隆中共鉴定出 6个线粒体DNA (mitochondrialDNA ,mtDNA)编码的基因 ,即ND 4L、ND 4、COX 2、Lys tRNA、ATP 8和ATP 6 .表明NO可以诱导食管癌细胞mtDNA编码的基因过表达 .
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     Purification of Cotton Mitochondria and Mitochondrial DNA
     棉花线粒体和线粒体DNA的分离与纯化
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     The Research Advance of Mitochondria DNA of Apidae
     蜜蜂(Apis)线粒体DNA(mtDNA)研究进展
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     On Classification of DNA
     DNA分类方法的探讨
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     That is rough DNA.
     95℃水浴加热10min,得到DNA粗提物。
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     migration of mitochondria.
     线粒体的迁移等过程.
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Overexpression of the genes induced by nitric oxide (NO) in EC109 esophageal carcinoma cell line was studied by using suppression subtractive hybridization (SSH), reverse mRNA dot blot and Northern blot. The nucleotide of their expressed sequence tag (EST) was sequenced and analyzed by NCBI database. The six mitochondrial DNA coding genes, ND 4L, ND 4, COX 2, Lys tRNA, ATP 8 and ATP 6 were identified from 69 SSH positive clones. The results indicated that NO can...

Overexpression of the genes induced by nitric oxide (NO) in EC109 esophageal carcinoma cell line was studied by using suppression subtractive hybridization (SSH), reverse mRNA dot blot and Northern blot. The nucleotide of their expressed sequence tag (EST) was sequenced and analyzed by NCBI database. The six mitochondrial DNA coding genes, ND 4L, ND 4, COX 2, Lys tRNA, ATP 8 and ATP 6 were identified from 69 SSH positive clones. The results indicated that NO can distinctly induce overexpression of mitochondria DNA encoded genes in the esophageal carcinoma cells. In addition, it was discovered that there were single nucleotide substitution in three sites of the fragment of ND 4L and ND 4 genes (10 736~11 449, 10 872 T→C, 11 001 A→G, 11 346 A→G), and one single nucleotide deletion (8 380,A) which will lead to occur the frame shift mutation in the peptide in the fragment of COX 2/ Lys tRNA / ATP 8/ ATP 6 genes (8 011~ 8 589 ). The analysis of amino acid sequences showed that an incorrect structural ATP 8 subunit existed possibly in the EC109 cell induced by NO. These results provided a new clue for further exploring the mechanism of NO effecting on carcinoma cells.

以人食管癌细胞系EC10 9作为驱赶方 (driver) ,以被一氧化氮 (nitricoxid ,NO)诱导的EC10 9作为实验方 (tester) ,应用抑制消减杂交 (suppressionsubtractivehybridization ,SSH)、反向mRNA斑点印迹和RNA印迹等技术手段研究了NO诱导的食管癌细胞中基因的过表达情况 .然后对过表达基因的表达序列标签 (expressedsequencetag ,EST)实施序列测定 ,并与GenBank进行BLAST同源性比较和序列突变分析 .结果先后两次从 6 9个SSH阳性克隆中共鉴定出 6个线粒体DNA (mitochondrialDNA ,mtDNA)编码的基因 ,即ND 4L、ND 4、COX 2、Lys tRNA、ATP 8和ATP 6 .表明NO可以诱导食管癌细胞mtDNA编码的基因过表达 .另外 ,在ND 4L/ND 4基因的片段 (10 736~ 114 4 9)上发现了三处同型单核苷酸置换 (10 872T→C ,110 0 1A→G ,11346A→G) ,在COX 2 /Lys tRNA/ATP 8/ATP 6基因片段 (...

以人食管癌细胞系EC10 9作为驱赶方 (driver) ,以被一氧化氮 (nitricoxid ,NO)诱导的EC10 9作为实验方 (tester) ,应用抑制消减杂交 (suppressionsubtractivehybridization ,SSH)、反向mRNA斑点印迹和RNA印迹等技术手段研究了NO诱导的食管癌细胞中基因的过表达情况 .然后对过表达基因的表达序列标签 (expressedsequencetag ,EST)实施序列测定 ,并与GenBank进行BLAST同源性比较和序列突变分析 .结果先后两次从 6 9个SSH阳性克隆中共鉴定出 6个线粒体DNA (mitochondrialDNA ,mtDNA)编码的基因 ,即ND 4L、ND 4、COX 2、Lys tRNA、ATP 8和ATP 6 .表明NO可以诱导食管癌细胞mtDNA编码的基因过表达 .另外 ,在ND 4L/ND 4基因的片段 (10 736~ 114 4 9)上发现了三处同型单核苷酸置换 (10 872T→C ,110 0 1A→G ,11346A→G) ,在COX 2 /Lys tRNA/ATP 8/ATP 6基因片段 (80 11~ 85 89)上发现了一处单核苷酸缺失(8380A) .氨基酸序列分析表明 ,在NO诱导的EC10 9中可能存在着一种结构异常的ATP 8肽链 (一条在N端被截短的只有 11个氨基酸残基的肽链 ,而正常的ATP 8肽链为 6 8个氨基酸残基 ) .这些研究结果为深入揭示NO对肿瘤细胞的作用机制提供了新的重要线索

 
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