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stellate cell line
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  星状细胞
     Methods HSC-T6 rat hepatic stellate cell line was chosen as the study model of the activated hepatic stellate cells.
     方法采用HSC-T6肝星状细胞系作为活化的肝星状细胞的研究模型。
短句来源
     In order to investigate the influence of interleukin 1β(IL 1β) and tumor necrosis factor α (TNF α) on gene expression of rat interstitial collagenase (MMP 13 ) in hepatic stellate cells, cells of a hepatic stellate cell line (HSC T6) were cultured in medium containing IL 1β or TNF α for different incubation periods. Total RNA of HSC was extracted and MMP 13 gene expression levels were measured by reverse transcription polymerase chain reaction.
     为了解白介素 1β(IL 1β)、肿瘤坏死因子α(TNF α)对大鼠肝星状细胞间质胶原酶 (MMP13 )基因表达的影响 ,在培养的肝星状细胞系中加入IL 1β、TNF α,于不同的时间点收集细胞 ,提取总RNA ,用逆转录定量PCR方法测定MMP13 的基因表达水平。
短句来源
     AIM: To study the effects of genistein (GE) and quercetin (QU) on proliferation, collagen synthesis, and procollagen messenger RNA (mRNA) expression of rat hepatic stellate cell line HSC-T6 cells.
     目的:研究金雀异黄素和槲皮素对HSC-T6大鼠肝星状细胞增殖和胶原合成及Ⅰ型原胶原mRNA表达的影响。
短句来源
     Objective: To study the effects of six flavonoids (fisetin, quercetin, apigenin, phloretin, hesperectin, and chalcone) on collagen synthesis of immortallized rat hepatic stellate cell line HSC-T6 cells.
     目的:研究黄颜木素、槲皮素、芹菜素、根皮素、橙皮素和查尔酮对血清、巨噬细胞培养上清液和转化生长因子β1刺激的大鼠肝星状细胞HSC-T6胶原合成的影响。
短句来源
     Objective To observe the effects of recombinant augmenter of liver regeneration (ALR) on the proliferation of he patic stellate cell line IG12 and ECM synthesis.
     目的 观察重组大鼠肝再生增强因子 (ALR)对肝星状细胞株IG12增殖及细胞外基质合成的影响 ,探讨其抗肝纤维化作用的可能机制。
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  “stellate cell line”译为未确定词的双语例句
     Objective:To study the effects of Anti-fibrosis Compound contained serum (AFCS) on procollagen type Ⅰ and Ⅳ (ProC-Ⅰ and ProC-Ⅳ), matrix metalloproteinase (MMP) and its tissue inhibitor (TIMP-1) gene expression in hepatic stellate cell line LI90 (HSC-LI90).
     目的 :探讨抗纤复方药物血清对肝星形细胞 (HSC)LI90细胞 (HSC LI90 )Ⅰ型、Ⅳ型前胶原和基质金属蛋白酶 (MMPs)及其组织抑制因子 (TIMP 1)基因表达的影响。
短句来源
     It can inhibit the proliferation and the production of extracellular matrix of hepatic stellate cell line IG12. The rALR can inhibit the production of extrace llular matrix, but not the proliferation of WFB.
     结论 原核细胞产生的rALR具有生物学活性 ,它能抑制大鼠肝肌纤维样细胞IG12的增殖及细胞外基质的产生 ; 对鼠成纤维细胞株WFB的增殖无显著抑制作用 ,能抑制WFB产生细胞外基质。
短句来源
     Objective: To study the effects of fisetin on proliferation and collagen synthesis of immortalized rat hepatic stellate cell line:HSCT6 cells.
     目的:研究黄颜木素对激活的永生型大鼠肝储脂细胞——HSC-T6细胞增殖和胶原合成的影响。
短句来源
     The effects of rALR on hepatic stellate cell line IG12 and rat fibroblas t cell line WFB were studied.
     于体外观察rALR对IG12及大鼠成纤维细胞株WFB增殖及细胞外基质合成的影响。
短句来源
     Hepatic stellate cell line IG12 may be valuable for screening the drugs which act as anti - liver fibrosis agents.
     IG12对于抗肝纤维化药物的筛选具有很大的价值。
短句来源
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  相似匹配句对
     Hepatic stellate cell apoptosis and mitochondrion
     肝星状细胞凋亡与线粒体
短句来源
     research progression of apoptosis of pancreatic stellate cell
     胰腺星状细胞凋亡研究进展
短句来源
     Establishment and application of liver stellate cell lines
     肝脏星形细胞系的建立及其应用
短句来源
     Cell Apoptosis
     略论细胞凋亡
短句来源
     Cell experiment:
     细胞实验:
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  stellate cell line
In this study we showed the in?vitro effects of Ras inhibition in a rat hepatic stellate cell line, HSC-T6.
      
Growth Arrest and Decrease of α-SMA and Type I Collagen Expression by Palmitic Acid in the Rat Hepatic Stellate Cell Line PAV-1
      
The aim of this work was to compare the behavior of two hepatic cell types involved in fibrogenesis: a liver stellate cell line (CFSC-2G) and primary hepatocytes, both in single and mixed cultures.
      
Proliferation, Functionality, and Extracellular Matrix Production of Hepatocytes and a Liver Stellate Cell Line: A Comparison Be
      
A methanol extract of Alsomitra macrocarpa leaves and branches induced a marked alteration of cell morphology in a human stellate cell line (LX-2).
      
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Objective: To study the effects of fisetin on proliferation and collagen synthesis of immortalized rat hepatic stellate cell line:HSCT6 cells. Methods: Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by 3Hproline incorporation. Results: Fisetin (6.25 ̄5000 mol/L)markly inhibited PDGFstimulated proliferation and TGF1stimulated collagen synthesis of HSCT6 cells in a concentrationdependent manner. Conclusion: Fisetin could inhibit...

Objective: To study the effects of fisetin on proliferation and collagen synthesis of immortalized rat hepatic stellate cell line:HSCT6 cells. Methods: Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by 3Hproline incorporation. Results: Fisetin (6.25 ̄5000 mol/L)markly inhibited PDGFstimulated proliferation and TGF1stimulated collagen synthesis of HSCT6 cells in a concentrationdependent manner. Conclusion: Fisetin could inhibit actived hepatic lipocyte proliferation and collagen synthesis

目的:研究黄颜木素对激活的永生型大鼠肝储脂细胞——HSC-T6细胞增殖和胶原合成的影响。方法:细胞增殖采用结晶紫染色法检测,胶原合成采用3H-脯氨酸掺入法分析。结果:黄颜木素(6.25~50.00μmol/L)以剂量依赖方式显著抑制血小板源生长因子(PDGF)刺激的HSC-T6细胞增殖,并抑制转化生长因子β1(TGFβ1)诱导的细胞内胶原合成。结论:黄颜木素具有抑制激活的肝储脂细胞增殖和胶原合成的作用。

Objective:To study the hepato protective and antifibrotic properties of silibinin, and its effects on proliferation and collagen synthesis of immortalized rat hepatic stellate cell line, HSC T6 cells. Methods: Cell proliferation was measured by cristal violet staining assay. Collagen synthesis was determined by 3H proline incorporation. Results: Silibinin (6.25 50 μg/ml) concentration dependently reduced the increase of cell proliferation and collagen synthesis of HSC...

Objective:To study the hepato protective and antifibrotic properties of silibinin, and its effects on proliferation and collagen synthesis of immortalized rat hepatic stellate cell line, HSC T6 cells. Methods: Cell proliferation was measured by cristal violet staining assay. Collagen synthesis was determined by 3H proline incorporation. Results: Silibinin (6.25 50 μg/ml) concentration dependently reduced the increase of cell proliferation and collagen synthesis of HSC T6 cells stimulated by serum, macrophage conditioned medium and cytokines platelet derived growth factor or transforming growth factor β 1. Conclusion: Inhibition of hepatic stellate cell proliferation and collagen synthesis may be one important mechanism of hepatoprotective and antifibrotic properties of silibinin. [

目的 :观察水飞蓟宾对大鼠肝贮脂细胞 HSC- T6增殖和胶原合成的影响 ,探讨其保护肝脏及抗硬化机制。 方法 :采用结晶紫染色法测定细胞增殖 ,3H-脯氨酸掺入法测定胶原合成。 结果 :水飞蓟宾 ( 6 .2 5~ 5 0μg/ m l)以浓度依赖方式抑制血清、巨噬细胞条件培养液以及血小板源生长因子或转化生长因子β1 诱导的细胞增殖和胶原合成。结论 :抑制贮脂细胞增殖和胶原合成可能是水飞蓟宾保护肝脏抗硬化机制之一。

Objective\ To investigate the effects of tumor necrosis factor-α (TNF-α) on stromelysin-1 and tissue inhibitor of metalloproteinase-1 (TIMP-1) gene expression in hepatic stellate cells. Methods\ The hepatic stellate cell line (HSC-T6) was cultured in medium containing TNF-α (30 μg/L) and the cells were collected at different incubation time points (8, 24, 48 and 72 h). Total RNA of HSC was extracted. The gene expression levles of stromelysin-1 and TIMP-1 were detected by using reverse transcription...

Objective\ To investigate the effects of tumor necrosis factor-α (TNF-α) on stromelysin-1 and tissue inhibitor of metalloproteinase-1 (TIMP-1) gene expression in hepatic stellate cells. Methods\ The hepatic stellate cell line (HSC-T6) was cultured in medium containing TNF-α (30 μg/L) and the cells were collected at different incubation time points (8, 24, 48 and 72 h). Total RNA of HSC was extracted. The gene expression levles of stromelysin-1 and TIMP-1 were detected by using reverse transcription polymerase chain reaction method. Results\ Stromelysin-1 gene expression level in TNF-α group was obviously higher than those in control group at the time points of 8, 24, 48 and 72 h, peaked at 24 to 48 h (2 to 3 times higher than in control groups)1. No significant difference in TIMP-1 gene expression level was found between the two groups at 8 to 48 h. However, TIMP-1 gene expression level in TNF-α group was increased remarkably at 72 h and was 3 times higher than those in the control group. Conclusion\ TNF-α could promote gene expression of stromelysin-1 and TIMP-1 in hepatic stellate cells.\;

目的 探讨肿瘤坏死因子 α(TNF α)对肝星状细胞基质分解素 1及金属蛋白酶组织抑制因子 1(TIMP1)基因表达的影响。方法 在培养的肝星状细胞系中加入 30 μg/LTNF α,于 8、2 4、48、72h 4个不同的时间点收集细胞 ,提取总RNA ;用逆转录定量聚合酶链反应 (PCR)方法测定基质分解素 1及TIMP1的基因表达水平。结果 TNF α组肝星状细胞基质分解素 1基因表达水平在 8、2 4、48、72h 4个时间点均明显高于对照组 ;2 4~ 48h达高峰 ,为对照组的 2倍。TNF α组肝星状细胞TIMP1的基因表达水平在 8、2 4、48h与对照组差异无显著性 (P >0 .0 5 ) ,72h显著升高 ,为对照组的近 3倍。结论 TNF α可增强肝星状细胞基质分解素 1及TIMP1基因的表达。

 
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