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fungal chromosomal
相关语句
  真菌染色体
     An efficient protocol for extraction of fungal chromosomal DNA
     硅胶破碎法抽提真菌染色体DNA
短句来源
     When we cloned fungal xylanase gene with the method of Genomic walking PCR,we constructed a simple and cheap protocol for extraction of fungal chromosomal DNA.
     在进行基因组步行PCR克隆真菌木聚糖酶基因的研究中,探索并建立了一套可在普通分子生物学实验室采用的高得率、高质量真菌染色体DNA的抽提方法.
短句来源
  “fungal chromosomal”译为未确定词的双语例句
     Southern hybridization analyses indicated that transformation takes place via the integration of plasmid DNA into the fungal chromosomal DNA. Multiple integrations occur producing tandem iterated arrays of plasmid molecules.
     DNA杂交分析结果表明,在正常转化子中质粒DNA以首尾相接、重复排列的形式整合入受体菌染色体DNA。
短句来源
  相似匹配句对
     An efficient protocol for extraction of fungal chromosomal DNA
     硅胶破碎法抽提真菌染色体DNA
短句来源
     Fungal rhinosinusitis
     真菌性鼻-鼻窦炎
短句来源
     The Chromosomal Changes of Neuroblastoma
     神经母细胞瘤的染色体改变
短句来源
     CHROMOSOMAL SITES AND CARCINOGENESIS
     染色体位点与癌变
短句来源
     Allergic fungal rhinosinusitis
     变应性真菌性鼻-鼻窦炎研究进展
短句来源
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  fungal chromosomal
Transformation took place via the integration of plasmid DNA into the fungal chromosomal DNA.
      
Southern hybridization analyses indicated that transformation takes place via the integration of plasmid DNA into the fungal chromosomal DNA.
      


The phytopathogenic fungu Helminthosporium setariae has been transformed to hygromycin B resistance by using a, plasmid (pAN7-l) containing the Escherichia coli hygromycin phosphotransferase gene (hph).When Hlb-2 was used as a recipient in transformation experiments with pAN7-l, large strongly growing HmB-resistant colonies appeared at frequency of 2/μg DNA. A second type of colony also developed on transformation plates. These appeared at higher frequencies, grew less vigorously and could not be subcultured...

The phytopathogenic fungu Helminthosporium setariae has been transformed to hygromycin B resistance by using a, plasmid (pAN7-l) containing the Escherichia coli hygromycin phosphotransferase gene (hph).When Hlb-2 was used as a recipient in transformation experiments with pAN7-l, large strongly growing HmB-resistant colonies appeared at frequency of 2/μg DNA. A second type of colony also developed on transformation plates. These appeared at higher frequencies, grew less vigorously and could not be subcultured in the presence of hygromycin B. They are believed to be abortive transformants. Southern hybridization analyses indicated that transformation takes place via the integration of plasmid DNA into the fungal chromosomal DNA. Multiple integrations occur producing tandem iterated arrays of plasmid molecules. Some transformants arose as heterokaryons. These could be resolved by propagation through single spore and transformants purified in this way remained mitotically stable.

本文报道了利用具有潮霉素抗性标记的质粒(pAN7-1)对粟长蠕孢菌原生质体进行转化的结果。经pAN7-1质粒DNA转化处理的粟长蠕孢菌原生质体在含潮霉素(200μg/ml)的选择性培养基上出现两类转化子。一类是正常转化子,其转化率为2个转化子/μg DNA;另一类是流产转化子,其产生频率为500—600个转化子/μg DNA。DNA杂交分析结果表明,在正常转化子中质粒DNA以首尾相接、重复排列的形式整合入受体菌染色体DNA。初筛获得的转化子多数以异核状态存在,经单孢分离纯化后可通过有丝分裂稳定传代。

When we cloned fungal xylanase gene with the method of Genomic walking PCR,we constructed a simple and cheap protocol for extraction of fungal chromosomal DNA.Genomic DNA was extracted and isolated from fungal mycelia by two slightly modified methods: liquid nitrogen grind and silica gel disruption together to increase the harvest of DNA,and spermidine treatment to increase the purity of DNA.The properties of the DNA were identified by agarose gel electrophoresis,restriction endonuclease digestion,PCR...

When we cloned fungal xylanase gene with the method of Genomic walking PCR,we constructed a simple and cheap protocol for extraction of fungal chromosomal DNA.Genomic DNA was extracted and isolated from fungal mycelia by two slightly modified methods: liquid nitrogen grind and silica gel disruption together to increase the harvest of DNA,and spermidine treatment to increase the purity of DNA.The properties of the DNA were identified by agarose gel electrophoresis,restriction endonuclease digestion,PCR reaction and U.V absorption rate.The result shows that this method can obtain high molecular weight DNA,which is so pure and thus is suited as substrate for restriction digestion and PCR reaction.

在进行基因组步行PCR克隆真菌木聚糖酶基因的研究中,探索并建立了一套可在普通分子生物学实验室采用的高得率、高质量真菌染色体DNA的抽提方法.采用液氮研磨和硅胶破碎相结合提高染色体DNA得率,采用亚精胺法纯化提高DNA的纯度.抽提的染色体DNA用琼脂糖凝胶电泳、限制酶切、PCR反应及紫外吸收光谱等方法进行了鉴定,结果显示此方法可以获得分子量大、样品纯的染色体DNA,可有效地用于PCR扩增,并能被限制酶有效地消化.

 
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