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fungal chromosome
相关语句
  真菌染色体
     A Rapid Method for Preparation of Fungal Chromosome DNA
     一种快速提取真菌染色体DNA的方法
短句来源
     A rapid method for preparation of fungal chromosome DNA was introduced. In this method,the solution containing 100mM Tris,100mM NaCl,50mM EDTA-Na2,and 2% SDS,pH 9.0 was used as the extraction buffer by crashing cell walls of fungi with quartz sand.
     介绍了一种适用于丝状真菌染色体DNA大片段的快速提取方法,该方法以(100mM Tris,100mM NaG l,50mMEDTA-Na22%SDS,pH值9.0)为提取液,经石英砂研磨破壁。
短句来源
     The article introduce a rapid method for preparation of fungal chromosome DNA In this method the quartz sand is used to break the fungal cell wall and the chromosome DNA is harvested rapidly in 1~2 h The method is applied successfully by the author to four kinds of fungi Neurospora crassa , Aspergillus oryzae , Morchella esculcnta , Saccharomyces cervisae All the chromosome DNA extracted has the fragment size lager than 20 kb and can be used directly for either digestion with restriction endoenzyme or PCR
     该方法用石英砂振荡破壁 ,快速便捷地提取真菌染色体DNA ,提取时间仅用 1~ 2h。 作者应用该方法成功地提取了粗糙脉胞菌 (Neurosporacrassa)、米曲霉 (Aspergillusoryzae)、羊肚菌 (Morchellaesculcnta)、酿酒酵母 (Saccharomycescervisae)等 4种不同真菌的染色体DNA ,所提DNA片段均大于2 0kb ,可直接用于限制性内切酶酶切、PCR扩增等分子生物学研究
短句来源
  相似匹配句对
     A Rapid Method for Preparation of Fungal Chromosome DNA
     一种快速提取真菌染色体DNA的方法
短句来源
     2 of chromosome.
     RET基因定位于 10号染色体长臂 1区 1带 2亚带(10all.2)。
短句来源
     were its chromosome markers.
     等为其标志染色体,未显示有完整Y染色体存在。
短句来源
     Fungal rhinosinusitis
     真菌性鼻-鼻窦炎
短句来源
     2.fungal granule;
     2霉菌颗粒;
短句来源
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  fungal chromosome
The DNA was stably integrated into the fungal chromosome.
      
The DNA was stably integrated into the fungal chromosome.
      
Southern hybridization of the genomic DNA from these mutants showed that they all had integrated pK45 at the pksL1 site on the fungal chromosome.
      
Homologous integration of pK45 into the pksL1 locus on the fungal chromosome.
      


The article introduce a rapid method for preparation of fungal chromosome DNA In this method the quartz sand is used to break the fungal cell wall and the chromosome DNA is harvested rapidly in 1~2 h The method is applied successfully by the author to four kinds of fungi Neurospora crassa , Aspergillus oryzae , Morchella esculcnta , Saccharomyces cervisae All the chromosome DNA extracted has the fragment size lager than 20 kb and can be used directly for either digestion with...

The article introduce a rapid method for preparation of fungal chromosome DNA In this method the quartz sand is used to break the fungal cell wall and the chromosome DNA is harvested rapidly in 1~2 h The method is applied successfully by the author to four kinds of fungi Neurospora crassa , Aspergillus oryzae , Morchella esculcnta , Saccharomyces cervisae All the chromosome DNA extracted has the fragment size lager than 20 kb and can be used directly for either digestion with restriction endoenzyme or PCR

介绍了一种适用于多种真菌染色体DNA的快速提取方法。该方法用石英砂振荡破壁 ,快速便捷地提取真菌染色体DNA ,提取时间仅用 1~ 2h。作者应用该方法成功地提取了粗糙脉胞菌 (Neurosporacrassa)、米曲霉 (Aspergillusoryzae)、羊肚菌 (Morchellaesculcnta)、酿酒酵母 (Saccharomycescervisae)等 4种不同真菌的染色体DNA ,所提DNA片段均大于2 0kb ,可直接用于限制性内切酶酶切、PCR扩增等分子生物学研究

A rapid method for preparation of fungal chromosome DNA was introduced.In this method,the solution containing 100mM Tris,100mM NaCl,50mM EDTA-Na2,and 2% SDS,pH 9.0 was used as the extraction buffer by crashing cell walls of fungi with quartz sand.As the results,the fragments with high qualitive chromosomal DNA were obtained from 5 species of fungi Neurospora crassa,Aspergillus oryzae,Penicillium chrysogenum,Cephalosporium sp..And the lengths of all chromosomal DNA fragments from fungi were longer than...

A rapid method for preparation of fungal chromosome DNA was introduced.In this method,the solution containing 100mM Tris,100mM NaCl,50mM EDTA-Na2,and 2% SDS,pH 9.0 was used as the extraction buffer by crashing cell walls of fungi with quartz sand.As the results,the fragments with high qualitive chromosomal DNA were obtained from 5 species of fungi Neurospora crassa,Aspergillus oryzae,Penicillium chrysogenum,Cephalosporium sp..And the lengths of all chromosomal DNA fragments from fungi were longer than 20 kb in nucleotides and were able to be used directly for either digestion with restriction endo-enzyme or PCR analysis.

介绍了一种适用于丝状真菌染色体DNA大片段的快速提取方法,该方法以(100mM Tris,100mM NaG l,50mMEDTA-Na22%SDS,pH值9.0)为提取液,经石英砂研磨破壁。应用该方法成功地提取了粗糙脉胞菌(Neurospora crassa)、米曲霉(Aspergillus oryzae)、产黄青霉(Penicillium chrysogenum)和头孢霉菌(Cep-halosporiumsp.)等4种不同丝状真菌的染色体DNA大片段,且所提DNA片段均大于20kb,可直接用于限制性酶切、PCR等分子生物学研究。

 
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