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africa green
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  非洲绿
    Objective:To investigate whether shiga toxin Ⅱ e varriant and ricin induce apoptosis in Africa green monkey kideny cell line Vero, and the expression of gene Caspase-9, Bax, Caspase-3.Methods : The raw Stx2e were isolated in the 40 to 80 fraction of ammoniumsulfate precipitation .
    目的:探讨志贺毒素Ⅱ型e突变体(shiga toxin Ⅱe varriant,Stx2e)和蓖麻毒素(ricin)诱导非洲绿猴肾细胞系Vero细胞凋亡的作用及其相关凋亡基因的表达。
短句来源
    This paper described the cell killing activity of toxin A of Clostridium difficile on four cell lines which were Vero(Africa green monkey kidney),TPC-1 (human thyroid papillary carcinoma),NIH 3T3 (mouse fibroblast)and NIH 3T3 ras (NIH 3T3 cell transfected with ras oncogene)cell line.
    本文报道艰难梭菌A毒素对4种培养细胞的细胞致死活性的探讨。 4种培养细胞为Vero(非洲绿猴肾细胞)细胞、TPC─1(人甲状腺肿瘤细胞)细胞、NIH3T3细胞(小鼠成纤维细胞)及将ras癌基因转基因于NIH3T3细胞的NIH3T3ras细胞。
短句来源
    3. Expression of CD81 gene in different eukaryotic cell linesThe recombinant vector pVAXl-CD81 and pcDNA3.1-CD81 were transfected into Africa green monkey kidney COS-7 cell line and human hepatocellular carcinoma HepG2 cell line respectively. And the transient expression products in the transfected cells were detected byimmunofluorescence assay (IFA), immunocytochemical staining andFACS.
    通过脂质体介导,将pVAXI一cD81转染至非洲绿猴肾细胞系COS一7细胞,pcDNA3.1一CD81转染至人肝癌细胞系H即仇细胞,采用间接免疫荧光法(IFA)检测CD81基因在COS一7细胞中的表达,采用免疫组织化学方法(胡C法)及流式细胞技术(FACS)定性及定量的检测CD81基因在HepGZ细胞表达状况。
短句来源
    The polymer having phenylalanine or tryptamine on its surface had low cell toxicity in low dosage and high transfection efficiency in Africa green monkey kidney cell line when R+/-=5.At last, PAMAM, which have Phe and Trp as its graft, could effect plasmid condensation and enhance transfection efficiency.
    在低的浓度下,聚合物有相对较弱的毒性,在电荷比例为5的时候,修饰Phe和Trp的聚合物能够大大提高绿色荧光蛋白在非洲绿猿肾细胞中的表达效率。
短句来源
  “africa green”译为未确定词的双语例句
    Methods pVAX1-GRA8 was purified and transfected into Africa green monkey kindey vero cell lines,expression of GRA8 in vero cell lines were detected by RT-PCR and immunoblot with rabbits sera infected with Toxoplasma gondii.
    方法:大量提取纯化重组真核表达质粒pVAX1-GRA8,瞬时转染培养的vero E-6细胞,RT-PCR和免疫印迹法检测GRA8在细胞中的表达。
短句来源
    ricin were Purified by a Sepharose 4B column and gel filtration on a Sephadex G-100. The Africa green monkey kideny cellline Vero were treated with raw Stx2e and ricin .
    方法:用硫酸铵沉淀法制备Stx2e突变体粗毒素; 经Sephrose 4B柱亲和层析和SephadexG—100凝胶过滤,分离得到ricin。
短句来源
    Methods:HSV-I strain Stocker was proliferated in Vero cells (Africa green monkey kidney cells) and the virus nucleic acid was extracted as template of PCR.
    方法 :采用Vero细胞培养HSV -IStocker株 ,并提取病毒DNA作为PCR模板。
短句来源
    The positive recombinant clone was characterized by PCR and digestion with restriction endonucleases. pVAC-GRA8 was transfected into Africa green monkey kindey Vero cell lines and expression of GRA8 in vero cell lines were detected by RT-PCR and immunoblot with rabbits sera infected with Toxoplasma gondii.
    将构建的真核重组表达质粒pVAC-GRA8转染vero细胞,分析转染vero细胞中GRA8的表达状况。
短句来源
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This paper described the cell killing activity of toxin A of Clostridium difficile on four cell lines which were Vero(Africa green monkey kidney),TPC-1 (human thyroid papillary carcinoma),NIH 3T3 (mouse fibroblast)and NIH 3T3 ras (NIH 3T3 cell transfected with ras oncogene)cell line. The methods to assess the cell killing activity including trypan blue exclusion test,MTS calorimetric assay, membrane damage test and fluorescence micrographs (morphology of cell nuclei)were performed on the four cell lines....

This paper described the cell killing activity of toxin A of Clostridium difficile on four cell lines which were Vero(Africa green monkey kidney),TPC-1 (human thyroid papillary carcinoma),NIH 3T3 (mouse fibroblast)and NIH 3T3 ras (NIH 3T3 cell transfected with ras oncogene)cell line. The methods to assess the cell killing activity including trypan blue exclusion test,MTS calorimetric assay, membrane damage test and fluorescence micrographs (morphology of cell nuclei)were performed on the four cell lines. The results showed the order of sensitivity of rounding of cells of four cell lines to toxin A as follows: NIH 3T3 ras,TPC-1,Vero,NIH 3T3.However, the order of sensitivity of cell killing activity of four cell lines to toxin A as follows TPC -1,NIH 3T3, Vero,NIH 3T3 ras. The results indicated the cell killing activity of toxin A on the four cell lines are not identified and not related to cell rounding of toxin A. Under the same dose of cell rounding of toxin A,the dose of cell killing activity of toxin A and the time-course of cell death were very different on the four cell lines. TPC-1 cell line is most sensitive to cell killing activity of toxin A. The dates suggest that the toxin A may have potential therapeutic value in the treatment of some cancers.

本文报道艰难梭菌A毒素对4种培养细胞的细胞致死活性的探讨。4种培养细胞为Vero(非洲绿猴肾细胞)细胞、TPC─1(人甲状腺肿瘤细胞)细胞、NIH3T3细胞(小鼠成纤维细胞)及将ras癌基因转基因于NIH3T3细胞的NIH3T3ras细胞。应用台酚蓝排除能试验、噻唑蓝(MTS)比色、细胞膜损害测定试验、荧光显微术观察细胞核的形态变化等测定A毒素细胞致死活性。实验表明:4种培养细胞系对A毒素细胞圆缩化作用的敏感性依次为NIH3T3ras,TPC─1,Vero,NIH3T3细胞。而对A毒素细胞致死活性的敏感性依次为TPC─1,NIH3T3,Vero,NIH3T3ras细胞。从而得知A毒素的细胞致死活性不但依细胞种类不同而不同,而且也不一定与A毒素的细胞圆缩化作用有关。肿瘤细胞TPC─1细胞对A毒素致死活性有特殊敏感性。以上结果对探索抗癌新药的研制具有重要意义。

Nontargeted mutagenesis refers to the mutagenesis that happens at undamaged base sites of DNA so as to differ from targeted mutagenesis and its mechanisms in mammalian cells remain to be verified.In this research,the dynamic characteristic of nontargeted mutagenesis induced with chemical mutagen MNNG in Africa green monkey kidney vero cells was illustrated first followed by blocking gene transcription and translation with RNA and protein synthetic inhibitor respectively at the time point generated the...

Nontargeted mutagenesis refers to the mutagenesis that happens at undamaged base sites of DNA so as to differ from targeted mutagenesis and its mechanisms in mammalian cells remain to be verified.In this research,the dynamic characteristic of nontargeted mutagenesis induced with chemical mutagen MNNG in Africa green monkey kidney vero cells was illustrated first followed by blocking gene transcription and translation with RNA and protein synthetic inhibitor respectively at the time point generated the highest level of nontargeted mutation.The results that the mutation frequency declined to control as transcription blocked while risen remarkably when translation blocked suggested the possibility that changes in gene expression involve in nontargeted mutagenesis and hinted the relationship between the signal transduction pathways with the mutagenesis in cells which has yet to be proved.

观察化学诱变剂甲基硝基亚硝胍(MNNG)引起的非洲绿猴肾vero细胞非定标性突变率的时相变化,继而在突变率最高时点分别以RNA合成抑制剂和蛋白质合成抑制剂阻断基因转录和翻译。结果发现前者能使MNNG引起的非定标性突变率下降到对照水平,而后者反而使突变率增高。提示非定标性突变形成过程中可能发生基因表达改变,并提出引起这种改变的细胞信号传导通路假设和今后的研究方向。

Objective: To clone HSV-I gD ectodomain gene HSV-I strain Stocker. Methods:HSV-I strain Stocker was proliferated in Vero cells (Africa green monkey kidney cells) and the virus nucleic acid was extracted as template of PCR. After the restriction endonucleases EcoRI and PstI were dealt with, the PCR products of gD gene fragment were inserted into pBV220 plasmid vectors at corresponding sites then transferred into E.coli DH5α. By PCR and restriction enzyme analysis the recombinant clone of gD gene was obtained...

Objective: To clone HSV-I gD ectodomain gene HSV-I strain Stocker. Methods:HSV-I strain Stocker was proliferated in Vero cells (Africa green monkey kidney cells) and the virus nucleic acid was extracted as template of PCR. After the restriction endonucleases EcoRI and PstI were dealt with, the PCR products of gD gene fragment were inserted into pBV220 plasmid vectors at corresponding sites then transferred into E.coli DH5α. By PCR and restriction enzyme analysis the recombinant clone of gD gene was obtained and named pBV220-gD. The nucleotide sequences of gD gene were determined by sequencing. Results:Comparing the clone with other HSV-I strains and HSV-Ⅱ we verified that they have homology of 99.77%, 86.55% respectively for nucleotide sequence and 99.31%, 88.28% for amino acid sequence. Conclusion:The clone of HSV-I gD gene might contribute to the expression of gD gene, preparation of recombinant gD as diagnostic antigen and subunit vaccine as well as be a meams of study for the structure and function of gD or gD receptor.

目的 :为了克隆单纯疱疹病毒I型 (HSV -I) gD膜外区基因。方法 :采用Vero细胞培养HSV -IStocker株 ,并提取病毒DNA作为PCR模板。PCR扩增出的gD片段经EcoRI和PstI双酶切 ,插入质粒pBV220 相应位点 ,转化E.coliDH5α。重组质粒经PCR、酶切鉴定命名为pBV220-gD。对克隆的HSV -IStocker株gD基因进行序列分析 ,并与其他HSV -I、Ⅱ gD基因相应部分进行比较。结果 :核苷酸序列同源性分别为99.77 %、86.55 % ,氨基酸序列同源性分别为99.31 %、88.28%。结论 :HSV -IgD基因的克隆为表达该基因 ,进一步研制重组抗原诊断试剂、研究亚单位疫苗及gD、gD受体的结构功能奠定了基础。

 
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