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double-mab
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  双抗体
     Study on application of a double-mAb sandwich ELISA to quantitatively detecting endotoxin
     双抗体夹心ELISA定量检测内毒素的方法学研究
短句来源
     Aim To establish a double-mAb sandwich ELISA for detecting bacteria endotoxin.
     目的  建立检测细菌内毒素(endotoxin, ET)的双抗体夹心ELISA法。
短句来源
     MethodsBy using two anti-LPS mAbs. C3A2and H3D3,as coating and enzyme-labelled antibody respectively, a double-mAb sandwich ELISA was developed.
     方法用自制的抗LPS-mAb C3A2和H3D3,分别作为包被和酶标记mAb,建立双抗体夹心ELISA法,并进行了实验条件的选择。
短句来源
     Methods The levels of interleukin-6 (IL-6) were measured by double-mAb sandwich ELISA,T-lymphocyte subsets (CD3+,CD4+ and CD8+) were also evaluated by direct immunofluorescence. IgG,IgA, IgM and C-reactive protein (CRP) in serum were detemined by rate nephelometry.
     方法采用双抗体夹心ELISA 法检测血清白细胞介素6受体(IL-6)。 直接免疫荧光法测定外周血T淋巴细胞亚群(CD3+,CD4+,CD8+),用速率散射比浊法检测免疫球蛋白、C反应蛋白(CRP),同时对患者进行血液流变学检测。
短句来源
  相似匹配句对
     Methods: The double mAb sandwich ELISA was used.
     方法:用双抗体夹心ELISA法测定NSE含量。
短句来源
     double alkanes.
     双链烷技术。
短句来源
     It has the double functions.
     它具有双重功能:消极功能为限制原告滥诉,积极功能为通过司法生成权利。
短句来源
     Study on application of a double-mAb sandwich ELISA to quantitatively detecting endotoxin
     双抗体夹心ELISA定量检测内毒素的方法学研究
短句来源
     Aim To establish a double-mAb sandwich ELISA for detecting bacteria endotoxin.
     目的  建立检测细菌内毒素(endotoxin, ET)的双抗体夹心ELISA法。
短句来源
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To evaluate the clinical value of detechon of serum CA125 level and itS halife in early and differential sts and prognosis of ovallan canceL Methods: The double mAb Sandwich ELISA was ed. Results: Uaing 40 u/ml of the cut-off value for malignant ovallan tumor, the sensitivity, specificity and accuracy of the ELISA for detection of sertun CA125 were 88. 5%, 87. 3% and 87. 7%, respeCtively. The posihve rates of serum CA125 detectal in sacs I, II, III and IV of pahents with ovarian cancer were succeSSively 60%,...

To evaluate the clinical value of detechon of serum CA125 level and itS halife in early and differential sts and prognosis of ovallan canceL Methods: The double mAb Sandwich ELISA was ed. Results: Uaing 40 u/ml of the cut-off value for malignant ovallan tumor, the sensitivity, specificity and accuracy of the ELISA for detection of sertun CA125 were 88. 5%, 87. 3% and 87. 7%, respeCtively. The posihve rates of serum CA125 detectal in sacs I, II, III and IV of pahents with ovarian cancer were succeSSively 60%, 75%, 93. 3% and 100%. The coincidence rates of serum CA125 level at 3 months after operation with diagnosis condrmed through the second and for exploratory operation respectively were 68. 7% and 72. 7%, and the coincidence rates Of half-life of CA125 with the diagneds were 100% and 90. 6%. Conclusion: Detectioni of serum CAl125 level is of signifecance in dmerentiation between benign and regnant Pelvic masses. Serum CAlf level and its posibye rate are closely related to clinical stages of overman cancer. However, detechon of half-life of CAlfs is better than its serum level for eshmahon Of recurrence of ovarian cancer and its residual foci in vivo.

目的:观察卵巢癌患者血清CA125在该病的早期诊断、鉴别诊断和术后监测中的意义。方法:应用单克隆抗体(mAb)双位点夹心ELISA一步法。结果:检测血清CA125诊断卵巢癌的敏感性、特异性和准确度分别为88.5%,87.3%和87.7%;其中I、II、III和IV期卵巢癌患者血清CA125的阳性率分别为60%,75%,93.3%和100%;术后3个月血清CA125的含量与第2,3次探查术确诊的符合率分别为68.7%和72.7%;CA125的半衰期与第2,3次探查术的符合率分别为100%和90.9%。结论:血清CA125的检测对盆腔包块良恶性的鉴别具有重要的意义。其含量双阳性率与临床分期密切相关;但其半衰期对卵巢癌的复发和术后残存灶的评估优于CA125含量测定。

Seven hybridma cell lines secreting monoclonal antibodies(mAb) to Salmonella paratyphi A flagellin protein were established by hybridma technique and three mAbs of them were characterized The results showed that mAb 2C8 and 5D7 were IgM(κ),mAb 2E6 was IgG(κ) They may recognize different epitopes on the flagellin protien and reacted only with S para typhi A and its flagellin protein, but not with other related enterobacteria including S niarem be, S dakar, S paralyphi B and C, S cholerae suis and...

Seven hybridma cell lines secreting monoclonal antibodies(mAb) to Salmonella paratyphi A flagellin protein were established by hybridma technique and three mAbs of them were characterized The results showed that mAb 2C8 and 5D7 were IgM(κ),mAb 2E6 was IgG(κ) They may recognize different epitopes on the flagellin protien and reacted only with S para typhi A and its flagellin protein, but not with other related enterobacteria including S niarem be, S dakar, S paralyphi B and C, S cholerae suis and S newport etc Immunoblotting ana lysis showed that 3 mAbs bound only to S papatyphi A flagellin protein of relative molecular mass(Mr) 52 000 A double mAb sandwich ELISA employing mAb 2E6(as coating) and 5D7(as enzyme labelled antibody) was used to detect S paratyphi A flagellin antigen in 13 samples from the patients with S paratyphi which blood culture was positive Twelve samples of them were positive and the coincidental rate was of 92 3%

本文采用杂交瘤技术,制备了7 株抗甲型副伤寒沙门氏菌鞭毛抗原单克隆抗体(m Ab) 的杂交瘤细胞株。对3 株mAb(2C8、2E6 和5D7) 进行了鉴定,mAb 2C8 和5D7 为IgM,2E6 为IgG1(κ)亚类,可分别识别不同的抗原表位,均具有很强的特异性,即只与甲型副伤寒沙门氏菌及其鞭毛抗原起反应,而与相关肠道细菌:尼亚里木沙门氏菌、达卡沙门氏菌及伤寒沙门氏菌、乙型和丙型副伤寒杆菌、猪霍乱沙门氏菌、纽波特沙门氏菌等均无交叉反应。免疫印迹显示,3 株mAb 只能与甲型副伤寒沙门氏菌鞭毛抗原Mr 为52 000 的蛋白结合。用mAb 2E6 和5D7 建立的双mAb 夹心ELISA 法,检测了13 例血培养阳性的副伤寒患者血清中的甲型副伤寒沙门氏菌鞭毛抗原,12 例呈阳性,阳性符合率为92-3% 。

ObjectiveTo evaluate the clinical value of detection of serum neuron specific enolase (S NSE) level and its half life in differential diagnosis, monitoring of chemotherapy and prediction of relapse of small cell lung cancer(SCLC).MethodsThe double mAb sandwich ELISA was used.ResultsUsing 20ng/ml of the cut off value for SCLC,the sensitivity, specificity, acuracy of the ELISA for detection of serum NSE were 81.8%, 91.6% and 89 5%, respectively....

ObjectiveTo evaluate the clinical value of detection of serum neuron specific enolase (S NSE) level and its half life in differential diagnosis, monitoring of chemotherapy and prediction of relapse of small cell lung cancer(SCLC).MethodsThe double mAb sandwich ELISA was used.ResultsUsing 20ng/ml of the cut off value for SCLC,the sensitivity, specificity, acuracy of the ELISA for detection of serum NSE were 81.8%, 91.6% and 89 5%, respectively. The positive rates of serum NSE detected in limited disease (LD) and extensive disease(ED) patients with SCLC were 72 7% and 90 9%. The patients whose serum NSE halflife were more than 20ds, the relapse would be found from 43~193d since they had been found to be SCLC, the sensitivity of prediction of relapse by half life of NSE was 83 3%.ConclusionDetection of serum NSE level is of significance in differentiation between SCLC and NSCLC. The half life of serum NSE predicted the relapse of SCLC were better than the detection of serum NSE. [

目的为阐明小细胞肺癌 (SCL C)患者血清神经元特异性烯醇化酶 (NSE)在该病的诊断、化疗和复发中的意义以及 NSE半衰期与 SCL C复发的关系。方法用单克隆抗体 EL ISA一步法测定了 NSE含量。结果 NSE诊断 SCL C的敏感性、特异性及准确度分别为 81.8%、91.6 %和 89.5 % ;其中局限性 (L D) SCL C和扩展性 (ED) SCL C患者的阳性率分别为 72 .7%和90 .9% ;NSE的半衰期大于 2 0 d者 ,其复发在发病 43~ 193d前即被发现 ,NSE半衰期预测 SCL C复发敏感性为 83.3%。结论血清 NSE检测对 SCL C与 NSCL C的鉴别诊断具有重要意义 ,NSE半衰期预测 SCL C复发的敏感性优于 NSE含量测定。

 
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