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bacteriophage lambda
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  λ噬菌体
     Bacteriophage lambda DNA was isolated from Escherichia coli W 1485 transfected with lambda bacteriophage.
     报道了λ噬菌体在大肠杆菌W1485中增殖、裂解的条件。
短句来源
  “bacteriophage lambda”译为未确定词的双语例句
     Effects of Ribosomal Protein Mutations on the Expression of Bacteriophage Lambda N Gene in Escherichia coli
     Escherichia coli 核糖体蛋白质突变影响Lambda噬菌体N基因的表达
短句来源
     Isolation and purification of bacteriophage lambda DNA
     λDNA的分离纯化
短句来源
     A mutant of bacteriophage lambda A,Nam7 can not grow in Escherichia coli strain A19 (met,thi,his-95,rna-19,rel-1) since an amber mutation is harboured in λNam7 besides temperature sensitive mutation cI857.λcI857 grows well on E.
     λ Nam 7除其cI基因是cI 857温度敏感突变外,其N基因携有amber无义突变,故在Eseherichia coli A19(met,thi,his-95,rna-19,rel-1)上不能增殖。
短句来源
     Bacteriophage Lambda EMBL4 was purified by density gradient centrifugation in cesium chloride Vector DNA was prepared by double digestion of purified EMBL4 DNA with Bam Hi/Sail.
     以氯化铯密度梯度离心法纯化噬菌体λEMBL4,将纯化的EMBL4 DNA用BamH1/SalI双酶切制成载体。
短句来源
     Bacteriophage Lambda EMBL4 was purified by step gradient centrifugation in glycerol. Vector DNA was prepared by double digestion of purified EMBL4 DNA with BamHI/ Sal I.
     用甘油分级梯度离心法纯化噬菌体入EMBL_4,将纯化的EMBL_4DNA用BamHI/SaLI双酶切。
短句来源
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  相似匹配句对
     Isolation and purification of bacteriophage lambda DNA
     λDNA的分离纯化
短句来源
     Factors Influencing Titer of the Bacteriophage Lambda DNA Packaging Extract
     影响λ噬菌体包装蛋白抽提物效价的因素
短句来源
     PREPARATION OF X BACTERIOPHAGE DNA
     λ噬菌体DNA的制备
短句来源
     Lambda Calculus with Type
     带类型λ-演算
短句来源
     The Progress in Bacteriophage Lysins
     噬菌体裂解酶研究进展
短句来源
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  bacteriophage lambda
coli bacteriophage lambda-gt11 (gt11) were identified by a monoclonal antibody (MAb), H19.47, against a putative transformation-related viral antigen consisting of a complex of three phosphorylated polypeptides, pp41, pp38, and pp24.
      
Oxolinic acid induces bacteriophage lambda similarly to other agents interfering with the normal replication cycle of the bacterial chromosome.
      
Induction of bacteriophage lambda with oxolinic and nalidixic acid
      
Interaction ofEscherichia coli O55 hybrids with bacteriophage lambda.
      
However, emetine decreased the adsorption of the bacteriophage lambda cI 857 onEscherichia coli C 600, probably due to interaction with cellular receptors.
      
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A mutant of bacteriophage lambda A,Nam7 can not grow in Escherichia coli strain A19 (met,thi,his-95,rna-19,rel-1) since an amber mutation is harboured in λNam7 besides temperature sensitive mutation cI857.λcI857 grows well on E.coli A19 since its A7 gene is wild type though cl is temperature sensitive.The only difference between λ,c1857 and λNam7 is a N gene.The plating efficiency or relative yield of λc1857 on A19 or its mutants could be used as index for estimation of the expression of its N gene.In...

A mutant of bacteriophage lambda A,Nam7 can not grow in Escherichia coli strain A19 (met,thi,his-95,rna-19,rel-1) since an amber mutation is harboured in λNam7 besides temperature sensitive mutation cI857.λcI857 grows well on E.coli A19 since its A7 gene is wild type though cl is temperature sensitive.The only difference between λ,c1857 and λNam7 is a N gene.The plating efficiency or relative yield of λc1857 on A19 or its mutants could be used as index for estimation of the expression of its N gene.In this paper 24 different ribosomal protein mutants were used for host to test the plating efficiencies and relative yield of λc1857.Results indicated that in ribosomal protein mutants S3+L22,S4+L16+L24,S21+L25,L24,no L1 and no L3,plating efficiencies were decreased to 10-6-10-6.In ribosomal protein mutants S3+S18+L6+L24+L27 and L27,the plating efficiencies were 6.02 and 3.56 times more respectively.The relative yields were changed correspondingly with the changes of plating efficiencies.All of these suggested that ribosomal protein mutations affect the expression of λ N gene.

λ Nam 7除其cI基因是cI 857温度敏感突变外,其N基因携有amber无义突变,故在Eseherichia coli A19(met,thi,his-95,rna-19,rel-1)上不能增殖。λcI 857只有cI 857温度敏感突变,其N基因是野生型,所以能以E. coli A19为宿主进行增殖。λcI 857和λ Nam7只有1个N基因之差。λcI 857在A19及其衍生株上增殖的优劣,可以作为判断N基因表达程度高低的标准。本文以24种核糖体蛋白质突变体为宿主,测定λcI 857的成斑率。结果在S3+L22,S4+L16+L24,S21+L25,L24,缺L1,缺S3等突变体中,成斑率下降到10~(-6)—10~(-6);在S3+S18+L6+L24+L27和L27突变体中,成斑率分别提高6.02和3.56倍。以上结果说明核糖体蛋白质突变影响λ N基因的表达。

This paper presents a procedure for construction of a genomiclibrary from human gastric carcinoma cell line MGC80-3. Large EcoRI partial digested MGC80-3 DNA fragments (15-20 Kb) are covalently joined to bacteriophage Lambda Charon 4A vectors and packaged into viable phage particles in vitro; Approximately 9.4 × 10 5 recombinant phages are formed per microgramme MGC80-3 DNA, and amplified by low density growth on agar plates to establish a permanent library of MGC80-3 cell line.

本文报道用Lambda Charon4A为载体构建人胃癌细胞系MGC80—3基因组文库的全过程。用限制性内切酶EcoRI不完全酶解MGC 80—3 DNA,分离获得15—20kb“目的”基因片段,再通过T4DNA连接酶的作用使之与Lambda Charon4A载体臂进行重组,经体外包装反应和Xgal呈色反应鉴定获得了9.4×10~5重组噬菌体,经低密度平皿扩增后,构建了一个人胃癌MGC 80—3细胞系基因组文库,为进一步开展胃癌基因的分离研究奠定了基础。

Bacteriophage Lambda EMBL4 was purified by density gradient centrifugation in cesium chloride Vector DNA was prepared by double digestion of purified EMBL4 DNA with Bam Hi/Sail. High molecular weight wild soybean DNA was extracted by CTAB method and was partially digested with Sau3A. The wild soybean insert DNA fragments ranging from 10 to 22 kb were recovered from agarose gel and joined with the EMBL4 vectors. The resulting recombinant DNA was packaged in vitro, and 8×10 pfu were obtained. This is completely...

Bacteriophage Lambda EMBL4 was purified by density gradient centrifugation in cesium chloride Vector DNA was prepared by double digestion of purified EMBL4 DNA with Bam Hi/Sail. High molecular weight wild soybean DNA was extracted by CTAB method and was partially digested with Sau3A. The wild soybean insert DNA fragments ranging from 10 to 22 kb were recovered from agarose gel and joined with the EMBL4 vectors. The resulting recombinant DNA was packaged in vitro, and 8×10 pfu were obtained. This is completely met with the theoretical value required f6r wild soybean genomic library. The library was screened by using α'-cDNA of cultivated soybean storage protein as a probe and one positive clone was obtained.

以氯化铯密度梯度离心法纯化噬菌体λEMBL4,将纯化的EMBL4 DNA用BamH1/SalI双酶切制成载体。用CTAB(十六烷基三甲基溴化铵)法提取野生大豆(种名待定)大分子DNA,Sau3A部分酶解,从琼脂糖凝胶中回收10—22kb“目的”DNA片段,与载体连接,体外包装成重组噬菌体。所得重组子值为8×10(?)pfu(噬菌斑形成单位),达到了构建野生大豆基因文库要求的理论值。以栽培大豆7S贮藏蛋白a′-cDNA作探针,用噬菌斑原位杂交法从文库中筛选出一个阳性克隆。

 
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