助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   hybrid protein 的翻译结果: 查询用时:0.01秒
图标索引 在分类学科中查询
所有学科
生物学
基础医学
肿瘤学
更多类别查询

图标索引 历史查询
 

hybrid protein
相关语句
  杂交蛋白
     Studies on the Preliminary Purification of Human IFNγ/TNFβHybrid Protein and Its Anti-Tumor Effect
     人IFNγ/TNFβ杂交蛋白的初步纯化及其抗癌效应研究
短句来源
     Utilizing recombinant HuIFN-γ/TNF-β hybrid protein (γTNFβ) prepared from micro-pore glass absorbed chromatography to treals human tumor cells in ritro, the results reveals that this hybrid protein has obvious antitumor effects on all three cell lines detected (human cervical cancer ME180, stomach eancer 7901 and lung cancer G6), and its killing rates are higher than that of either IFN-γ or TNFβ alone at same dose.
     用MPG吸附层析法所制备的HuIFN-γ/HuTNFβ重组双功能杂交蛋白(简称γTNFβ)[1]去处理人宫颈癌细胞株ME180、胃癌细胞株7901和肺癌细胞株G6,结果表明此杂交蛋白对这三个细胞株都有明显的杀伤作用,其杀伤率显著高于同剂量的IFN-γ或TNF-β;
短句来源
     The hybrid protein has appreciable killing effect against human tumor cell lines(such asME 180,7901 as well as G_6)in vitro and its cytotoxic action is significantly stronger than that of single IFNγor TNFβ.
     该杂交蛋白在体外对人宫颈癌细胞株(ME180),人胃癌细胞株(7901)和人肺癌细胞株(G6)均有明显的杀伤作用,并都显著地高于单一的IFNγ或TNFβ对这些肿瘤细胞的杀伤。
短句来源
     PRELIMINARY PURIFICATION OF HUMAN IFNγ/ TNFβ HYBRID PROTEIN WITH MPG
     HuIFN-γ/HuTNF-β重组双功能杂交蛋白的分离和初步纯化
短句来源
     A recombinant hybrid protein──human interferon γ/tumor necrosis factor β(Hu IFNγ / TNFβ, γTNFβ) expressed by the extra fusion gene in E. coli HB 101 was identified and purified by means of micropore glass absorbed chromatography(MPG).
     本文对E.coliHB101菌株的外源基因表达产物"人γ-干扰素/人β-肿瘤坏死因子重组双功能杂交蛋白"(HuIFN-γ/HuTNF-β简称γTNFβ)进行了分离和初步纯化,并将产物作了抗病毒和细胞毒活性检测。
短句来源
更多       
  杂合蛋白
     The cytotoxicities of human recombinant γTNFβ (IFNγ ITNFβ hybrid protein)^ TNF P and IFN Y were measured on murine cell line L929 and human cell line Hela. L929 Cell is sensitive to TNF 3 and Hela is resistant to it.
     本文检测了γTNFβ(IFNγ/TNFβ的杂合蛋白)、TNFβ和IFNγ对TNF的敏感靶细胞L929和抗性靶细胞Hela的细胞毒效应。
短句来源
     IMMUNOREGULATORY PROTERTIES OF RECOMBINANT HUMAN IFN/TNFβ HYBRID PROTEIN
     人干扰素/β肿瘤坏死因子重组杂合蛋白的免疫调节功能
短句来源
     CYTOTOXIC EFFECT OF A HYBRID PROTEIN INTERFE RON γ/TUMOR NECROSIS FACTOR β ON INTERFERON γRESISTANT CELL LINES IN VITRO
     人γ干扰素/β肿瘤坏死因子重组杂合蛋白对γ干扰素抗性细胞株的杀伤效应
短句来源
     The regulatory effects of rhIFN/rhTNFβ on immune responses of normal animals. The results are summarized as follows: When LPSactivated peritoneal macrophages of guinea pigs were incubated with this hybrid protein, the concentration of H2O2 released by macrophages in cellfree supernatant was found to be much higher than that in controls.
     报道了人干扰素/β肿瘤坏死因子(rhIFN-/rhTNF-β)杂合蛋白对正常动物免疫反应的影响,结果如下:经LPS激活的豚鼠腹腔巨噬细胞,当与适宜剂量的杂合蛋白温育后,其无细胞上清液中的H2O2浓度高于对照组。
短句来源
     THE CYTOTOXIC EFFECT OF A HYBRID PROTEIN γ TNF β ON IFN γ RESISTANT CELL LINES IN VITRO
     人γ干扰素/β肿瘤坏死因子重组杂合蛋白对γ干扰素抗性细胞株的杀伤效应
更多       
  “hybrid protein”译为未确定词的双语例句
     The development of a recombinant hybrid protein of Plasmodium falciparum and analysis of its antigenicity and protectivity
     The development of a recombinant hybrid protein of Plasmodium falciparum and analysis of its antigenicity and protectivity
短句来源
     Consturction, Expression and Characterization of the Hybrid Protein Which Fused Peptide 12 Derived from Hirudin to Low Molecular Weight Single-Chain Urokinase
     水蛭素12肽与低分子量单链尿激酶融合基因的构建、表达及产物特性分析
短句来源
     We showed that the hybrid protein retained the binding reactivity for the TAG72 antigen as the original B72.3 antibody, and the metal binding capacity of the mative metallothionein molecule.
     研究表明,杂交分子B723MT1同时保留了原来B723抗体对TAG72抗原的亲和力和天然MT1分子的金属结合能力。
短句来源
     The expression vector was transfected to the heavy chain loss mutant B72.3 mut(k) cell line. The hybrid protein B72.3MT1 was purified from transfectant supernantes using a protein G column.
     这种表达质粒含有选择标识物neo基因、鼠免疫球蛋白促进子和增强子、鼠金属硫因1(MT1)、cDNA基因与B723重链CH2功能区基因相结合的基因片段,以此质粒转染重链缺失突变型B723Mut(k)细胞株,转染上清液通过蛋白G柱纯化杂交分子B723MT1。
短句来源
     The antioxidant activity of different subunits of hybrid protein and nativeallophycocyanin were studied in different vitro experimental models.
     本研究通过以下模型比较了六种重组别藻蓝蛋白和天然别藻蓝蛋白在体外不同抗氧化模型中的活性氧清除作用:
短句来源
更多       
查询“hybrid protein”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  hybrid protein
Two-hybrid protein interaction screening in yeast was used to identify proteins that interact with the HBx nonstructural protein of hepatitis B virus (HBV).
      
Mutant and hybrid protein derivatives for the experimental therapy with plasminogen activators (review)
      
Sera from patients with HAM showed significantly higher activities of antibodies against HTLV-I-gag-env hybrid protein than sera from other HTLV-I-infected individuals including ATLL patients.
      
Activity of a recombinant transforming growth factor-α-Pseudomonas exotoxin hybrid protein against primary human tumor colony-fo
      
They had different albumins, transferrins, and hemoglobins; frogs with hybrid protein patterns were not found in regions where the two species are sympatric.
      
更多          


In a previous paper, we reported 10 genetic recombinants of FMDV and EV isolated by dual infection of IBRS-2 cell culture with these two viruses. A cloned strain, c731, was obtained, which possessed some properties of both FMDV and EV.In this paper we report the results of further characterization of c73l strain.Vpl of c731 capsid was found to be a hybrid protein reacting with both EMDV and EV antisera. The virulence of c731 for mice was mardekly lower than that of the original FMDV strain. Inoculation...

In a previous paper, we reported 10 genetic recombinants of FMDV and EV isolated by dual infection of IBRS-2 cell culture with these two viruses. A cloned strain, c731, was obtained, which possessed some properties of both FMDV and EV.In this paper we report the results of further characterization of c73l strain.Vpl of c731 capsid was found to be a hybrid protein reacting with both EMDV and EV antisera. The virulence of c731 for mice was mardekly lower than that of the original FMDV strain. Inoculation with c731 resulted in antibody production and protection against challenge with virulent FMDV virus.

对不同属间小RNA病毒基因重组株c731,进行了病毒与细胞亲和力、毒蛋白的PAGE,病毒的乳鼠毒力和免疫原性试验,进一步证实,c731是口蹄疫病毒和猪肠道病毒通过基因重组产生的新病毒株。

We show that the fusion between the 3'-end coding sequences of Hu-IFN-aA gene and the 3'-end coding sequences for C-terminal 16 amino acids of Hu-IFN-y was formed by oligonucleotide directed mutagenesis.Under the control of phage A PL promoter,the hybrid protein Hu-IFN-αA/γ was expressed. This molecule has been purified by the use of monoclonal anti-body against Hu-IFN-α. The purified protein mainly exhibited a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and has anti-viral...

We show that the fusion between the 3'-end coding sequences of Hu-IFN-aA gene and the 3'-end coding sequences for C-terminal 16 amino acids of Hu-IFN-y was formed by oligonucleotide directed mutagenesis.Under the control of phage A PL promoter,the hybrid protein Hu-IFN-αA/γ was expressed. This molecule has been purified by the use of monoclonal anti-body against Hu-IFN-α. The purified protein mainly exhibited a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and has anti-viral activity on bovine MDBK celis, The hybrid Hu-IFN-αA/γ can be phosphorylated by [γ-32P] ATP and cAMP-dependent protein kinase. In competition assays, recombinant Hu-IFN-αA displaced 70% of [γ-32P] -IFN-αA/γ binding to MDBK celis

使用寡核苷酸指导的定点突变方法,将人αA型干扰素的完整基因与γ干扰素C端16个氨基酸的编码序列融合,在噬菌体λP_L启动子控制下,合成了一个杂交蛋白质。此蛋白质经抗人α干扰素单克隆抗体纯化后,在MDBK细胞上具有抗病毒活性,并像γ干扰素一样,可被依赖于cAMP的蛋白激酶磷酸化。[γ-~(32)P]杂交蛋白与MDBK细胞的结合,可被αA型干扰素大大抑制(70%)。

The present investigation has been earried out to study hCG B-subunit C-terminal containing hybrind protein synthesized by λgt11 hCG Y1089 through genetic engineering technology. A 36 peptide gene containing h CG B-subunit C-terminal has been artificially synthesized by Oligonucleotide Synthesizer, the double stranded DNA was cloned into an expression vetor λgt11 and the 36 peptide gene fusion product was successfully expressed in host strain E. coli Y1089. h CG B-subunit C-terminal 36 peptide containing hybrid...

The present investigation has been earried out to study hCG B-subunit C-terminal containing hybrind protein synthesized by λgt11 hCG Y1089 through genetic engineering technology. A 36 peptide gene containing h CG B-subunit C-terminal has been artificially synthesized by Oligonucleotide Synthesizer, the double stranded DNA was cloned into an expression vetor λgt11 and the 36 peptide gene fusion product was successfully expressed in host strain E. coli Y1089. h CG B-subunit C-terminal 36 peptide containing hybrid protein (B-galactosidase-h CG) expressed in λgt11 hCG Y1089 was precipitated in the ammonium sulfate solution of 50% saturation, affinity isolated by anti-B-galactosidase sepharose 4B column and finally purified with preparative polyacrylamide gel electrophoresis. Comparative analysis of different preparations revealed that the eluate of affinity column gave a mjor band and two minor bands, and the eluate of preparative polyacrylamide gel electrophoresis only gave one band while the solution of ammonium sulfate precipitated gave more than 30 bands in polyacrylamide gel electrophoresis, it indicated that anti-B-galactosidase affinity chromatography and preparative polyacrylamide gel eleetrophoresis can be used for isolating and purifing B-galactosidase-h CG. The results of western blot technique demonstrated that B-galactosidase-hCG contains immunological property of hCG B-subunit C-terminal. Preliminary studies of antigenicitY of this B-galactosidase-hCG through counter current immunoelectrophoresis and double immunodiffusion techniques have clearly demonstrated that this Purified B-galactosidase-hCG can be crossly immunopreciPitated with rabbit anti-B-galactosidase-hCG serum, it has been concluded that B-galactosidase-hCG possesses the antigenieitY, but the biological activity of this this hybrid protein to neutralizing hCG has to be determined by in vitro or in vivo system.

通过寡核苷酸合成仪,用化学方法合成相当于hcGβ链C末端36肽基因的片段,成功地克隆到噬菌体载体λgt11中,并在宿主菌株E. coli y_(1089)中得到表达。含λgt11 hCG重组体的E. coli y_(1089)所合成的含人类绒毛膜促性腺激素β链c末36肽的杂交蛋白(下称:β—galactosidase—hCG),通过硫酸铵沉淀、亲和层析和制备性聚丙烯酰胺凝胶电泳,可得电泳纯单一色带的β—gaIactosidase—hCG。蛋白质转印(Western Blot)技术证实β—galactasidase—hCG具有hCGβ链c末端36肽的免疫特性。对流免疫电泳和免疫双扩散试验结果表明β—galactosidase—hCG具免疫原性。从一立升培养液的菌体中可分离纯化约3.8毫克的β—galaetosidase—hCG。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关hybrid protein的内容
在知识搜索中查有关hybrid protein的内容
在数字搜索中查有关hybrid protein的内容
在概念知识元中查有关hybrid protein的内容
在学术趋势中查有关hybrid protein的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社