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   isopropyl thiogalactoside 的翻译结果: 查询用时:0.143秒
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isopropyl thiogalactoside
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  “isopropyl thiogalactoside”译为未确定词的双语例句
     Fusion protein was expressed in Escherichia coli by the isopropyl thiogalactoside (IPTG) induction.
     用IPTG(异丙基-硫代-β-D-半乳糖苷)诱导融合蛋白在大肠杆菌中表达;
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  相似匹配句对
     Isopropyl Palmitate
     棕榈酸异丙酯
短句来源
     Fusion protein was expressed in Escherichia coli by the isopropyl thiogalactoside (IPTG) induction.
     用IPTG(异丙基-硫代-β-D-半乳糖苷)诱导融合蛋白在大肠杆菌中表达;
短句来源
     Determination of Isopropyl Chloroformate
     氯代甲酸异丙酯含量的测定方法研究
短句来源
     coli BL21De3 that was consequently induced to express by isopropyl-β-D-thiogalactoside(IPTG).
     coli BL21DE3宿主菌,通过IPTG诱导表达,分离、纯化及Western印迹法鉴定。
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  isopropyl thiogalactoside
Thus, their expression is dependent upon the amount of inducer isopropyl thiogalactoside (IPTG) in the medium.
      
The cloned antifreeze protein gene was expressed in Escherichia coli, and a fusion protein of about 60?kDa was detected after isopropyl thiogalactoside induction.
      
Lane 1, recombinant human IGF-II; lanes 2 and 3, cell lysate from two different clones after induction with isopropyl thiogalactoside.
      
Expression was carried out according to the instructions supplied with the cells with a final induction of 20 h at 4 mm isopropylthio-galactoside.
      
Expression of protein was induced for 3 h in midlog-phase using 0.4 mM isopropyl-thiogalactoside.
      
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Objective To characterize VP4 gene of human rotavirus field strain in China and obtain highly expressed VP4 protein Methods Full length VP4 gene of human rotavirus CR188 strain was amplified by RT PCR and cloned into prokaryotic expression plasmid pET21a(+): (1) The VP4 gene was sequenced with terminal termination method and compared with previously published strains (2) The recombinant plasmid was transformed into E coli BL21 (DE3) and induced by isopropyl thiogalactoside (IPTG) The expressed...

Objective To characterize VP4 gene of human rotavirus field strain in China and obtain highly expressed VP4 protein Methods Full length VP4 gene of human rotavirus CR188 strain was amplified by RT PCR and cloned into prokaryotic expression plasmid pET21a(+): (1) The VP4 gene was sequenced with terminal termination method and compared with previously published strains (2) The recombinant plasmid was transformed into E coli BL21 (DE3) and induced by isopropyl thiogalactoside (IPTG) The expressed protein was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and Western blot Results (1) The cloned VP4 gene was 2359 bp in length and had a long open reading frame (ORF) coding for 775 amino acids Comparative analysis of the deduced amino acid sequences of VP4 indicates a high degree of homology among the same serotypes (94% 98%) and a low degree of homology among the various serotypes (64% 78%) (2) The maximal quantity of expressed protein was harvested at the hours 2 3 following induction and showed 11 % of expression level in the whole cell protein Monoclonal antibodies against group A rotavirus VP4 could specifically recognize the expressed protein epitopes in Western blot assay Conclusion Sequence analysis and laboratory diagnosis using clinical sample indicated consistently that the serotype of CR188 was P1A; The VP4 gene was highly expressed in prokaryotic system and with the same antigenicity of natural VP4

目的 研究轮状病毒(RV) 我国地方株VP4 基因特点,并获得高效表达的VP4。 方法采用RTPCR 技术扩增RVP1A 型我国地方株CR188 的完整VP4 基因,克隆到原核表达质粒pET21a(+ )中:(1)用末端终止法进行核苷酸序列测定,并与人(HRV)标准株作比较分析。(2) 重组质粒转化原核表达宿主菌E-coli BL21(DE3) ,异丙硫半乳糖甙(IPTG) 诱导表达,经十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDSPAGE) 和Western blot 对其表达产物进行鉴定。结果 (1)CR188 VP4 基因全长2359bp,含编码775 个氨基酸的单一的开放读码框架(ORF) ,与相同血清型标准株之间氨基酸序列具有高度同源性(94 % ~98% ),与不同血清型标准株间相比较,则同源性较低(64 % ~78% ) 。(2) 重组VP4表达量在诱导2~3 小时时最高,占细胞总蛋白的11 % ;表达蛋白与小鼠抗A组RVVP4 P1A型特异性单克隆抗体( 单抗) 发生反应。结论 序列分析预测血清型的结果与临床标本的实验室诊断一致,CR188 VP4 为P1A型;VP4 全基因在原核系统中获得表...

目的 研究轮状病毒(RV) 我国地方株VP4 基因特点,并获得高效表达的VP4。 方法采用RTPCR 技术扩增RVP1A 型我国地方株CR188 的完整VP4 基因,克隆到原核表达质粒pET21a(+ )中:(1)用末端终止法进行核苷酸序列测定,并与人(HRV)标准株作比较分析。(2) 重组质粒转化原核表达宿主菌E-coli BL21(DE3) ,异丙硫半乳糖甙(IPTG) 诱导表达,经十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDSPAGE) 和Western blot 对其表达产物进行鉴定。结果 (1)CR188 VP4 基因全长2359bp,含编码775 个氨基酸的单一的开放读码框架(ORF) ,与相同血清型标准株之间氨基酸序列具有高度同源性(94 % ~98% ),与不同血清型标准株间相比较,则同源性较低(64 % ~78% ) 。(2) 重组VP4表达量在诱导2~3 小时时最高,占细胞总蛋白的11 % ;表达蛋白与小鼠抗A组RVVP4 P1A型特异性单克隆抗体( 单抗) 发生反应。结论 序列分析预测血清型的结果与临床标本的实验室诊断一致,CR188 VP4 为P1A型;VP4 全基因在原核系统中获得表达,表达的重组VP4 抗原可被型特?

AIM: To get large amounts of pure antigens to raise specific antibodies and to perform quantifications.METHODS: CYP2B6 (cytochrome P) cDNA fragments was ligated into BamHI restricted PGEX-3b to generate recombinants PGEX/2B6. We identified recombinants PGEX/2B6 by EcoRI digestion. The expression of fusion proteins were induced by adding isopropyl-thiogalactoside(IPTG). Several clones showed high-level expression of fusion proteins. Insoluble proteins was isolated from the bacteria and the fusion...

AIM: To get large amounts of pure antigens to raise specific antibodies and to perform quantifications.METHODS: CYP2B6 (cytochrome P) cDNA fragments was ligated into BamHI restricted PGEX-3b to generate recombinants PGEX/2B6. We identified recombinants PGEX/2B6 by EcoRI digestion. The expression of fusion proteins were induced by adding isopropyl-thiogalactoside(IPTG). Several clones showed high-level expression of fusion proteins. Insoluble proteins was isolated from the bacteria and the fusion proteins was recovered and purified from a preparative (2mm) SDS-PAGE. The polyarcrylamide gel containing the fusion proteins glutathione S-transferase(GST-2B6) were used to immunize BALB/C mice from which polyclonal ascites fluid was prepared. The purified fusion proteins GST-1A1(GST fusion protein of CYP1A1 cDNA246~386aa expressed in this library ,purified by preparative SDS-PAGE), GST-2B6 were used to test the specificity of 2B6pAb. RESULTS:Fusion proteins constructed between GST and CYP2B6 was expressed in Escherichia coli DH5α. Mouse antibodies are raised against the fusion proteins GST-2B6. 2B6pAb was fond to be specific antibody.CONCLUSION:Recombinant PGEX/2B6 were constructed and purified fusion proteins GST-2B6, and specific 2B6pAb were obtained.

目的 :获得纯化抗原用于制备CYP2B6多克隆抗体。方法 :PCR扩增目的基因片段 ,亚克隆入融合蛋白表达载体pGEX - 3b ,构建了重组质粒pGEX/ 2B6。然后将该重组质粒转化大肠杆菌DH5α ,IPTG诱导表达 ,SDS -PAGE分离 ,获纯化融合蛋白GST - 2B6。用GST - 2B6免疫BALB/C小鼠 ,自腹水中获取CYP2B6多克隆抗体 (2B6pAb)。结果 :融合蛋白GST - 2B6 (CYP2B6 2 0 2~ 35 2aa) ,并获其特异的多克隆抗体。结论 :通过构建融合蛋白重组表达质粒pGEX/ 2B6 ,用获得的初步纯化融合蛋白GST - 2B6制备了特异的2B6pAb

To obtain sonic hedgehog (SHH) and to investigate its function in neurodegenerative disorders, reverse transcription-polymerase chain reaction (RT-PCR) methods were used to clone cDNA fragment of N terminal of SHH(SHH-N) gene from different tissues of Wistar embryonic rats at various stages. The acquired cDNA was subcloned into pGEM-T/pQE30 vectors and recombinants were then identified by sequencing. The expression of SHH-N protein was then induced by isopropyl thiogalactoside (IPTG). It had been found...

To obtain sonic hedgehog (SHH) and to investigate its function in neurodegenerative disorders, reverse transcription-polymerase chain reaction (RT-PCR) methods were used to clone cDNA fragment of N terminal of SHH(SHH-N) gene from different tissues of Wistar embryonic rats at various stages. The acquired cDNA was subcloned into pGEM-T/pQE30 vectors and recombinants were then identified by sequencing. The expression of SHH-N protein was then induced by isopropyl thiogalactoside (IPTG). It had been found that the gene sequence showed some nuances as compared with that submitted to the PUBMED databank and the rat SHH-N protein of which the molecular weight is 21ku was expressed after induction. The results suggest that: SHH-N is a development-related gene and it is highly homologous among different species and could be normally expressed in prokaryotic system.

为了获得SHH蛋白以研究它在治疗神经变性疾病中的作用 ,本室提取不同胚龄Wistar胎鼠中的总RNA ,应用RT PCR技术获得SHH N的cDNA ,重组于pGEM T载体 ,经测序鉴定后 ,构建原核表达质粒并获得表达菌株Q15 SHH N ,然后诱导SHH蛋白的表达。结果发现 ,在E11.5 E2 0 .5胎鼠的脊索中能克隆到 6 30bp的cDNA片段 ,并且随胚龄增加 ,此片段的表达量逐渐减少 ;测序结果显示 ,此片段及其所编码的氨基酸序列同数据库中人、小鼠和SD大鼠的SHH N的序列有较高同源性 ;表达菌株经诱导后产生约 2 1kD的融合蛋白。提示SHH N是一种发育相关基因 ,在不同种属中的同源性较高 ,并且在原核系统中能进行表达

 
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