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   rotary shaker 的翻译结果: 查询用时:0.009秒
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rotary shaker
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     The optimal fermentation conditions were investigated: initial pH7.0, temperature 33℃, with 2% citrus peel powder and 1% lactose as carbon source, 1% (NH4)2SO4 and 0.3% yeast extract paste as nitrogen source, after fermenting for 32h on a rotary shaker.
     其最适发酵条件为:以2%桔皮粉+1%乳糖作碳源,以1%(NH4)2SO4+0.3%酵母膏作氮源,在起始pH7.0,33℃摇床发酵32h左右达产酶高峰。
短句来源
     The other conditions: volumeof medium is 100ml/250ml, culture temperature is 30℃, the speed of rotary shaker is150r/min.
     摇瓶装液量为100ml/250 ml,培养温度为30℃,摇床转速为150r/min。
短句来源
     The composition of a medium is as following (g/L): glucose 80, yeast extract 1.25, KH2PO4 3.0, MgSO4 - 7H2O 0.05, CaCO3 10.0, pH 6.0. When culture incubated on rotary shaker at 30℃ for 6 days, The yield of extracellular polysaccharide reached 17.38 g/L.
     培养基组成(g/L):葡萄糖80.00,酵母膏1.25,KH_2PO_3.00,MgSO_4·7H_2O0.05,CaCO_3 10.00,pH6.0。 在30℃,旋转式摇床(200r/min)上培养6天。
短句来源
     The preferred conditions:the initial pH of medium is 4.2,culture temperature is 32~C,the speed of rotary shaker is 120r/min,inoculum size is 7.5%and inoculation time is 48h.
     最适培养条件为:培养基的起始pH为4.2,培养温度为32℃,摇床转速为120r/min,接种量为7.5%,种子种龄为48小时。
短句来源
     The cultivation was carried out by rotary shaker(250 r/min) at (29±1) ℃ with initial pH 7.5 for 96 h, during which 0.4 g/dL peanut oil was fed at 36, 54, and 72 h respectively.
     发酵培养基起始 pH 7.5,发酵温度 (2 9± 1 )℃ ,摇床转速 2 50r/min ,发酵周期 96h ,发酵期间于 3 6,54,72h分别流加 0 .4 g/dL花生油 .
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  “rotary shaker”译为未确定词的双语例句
     The medium for fermentation consisted of 1% tryptone, 0.5% yeast extract, 5% sweet potato meal, 0.4% Na2HPO4 · 12H2O, 0.03% KH2PO4, 0.1% Na2CO3. The strain produced maximux alkaline protease activity after growth at 42℃ for 44-48 h on a rotary shaker.
     适宜的发酵条件:培养基(%)胰蛋白胨1,酵母膏0.5,玉米粉5,Na_2HP0_4·12H_20 0.4,KH_2P0_4 0.03,Na_2CO_3 0.1,自然pH。
短句来源
     Under these conditions,the yield ofexopolysaccharide was as high as 23.2 g/L on rotary shaker at 25℃ for 108 h.
     在上述条件下,25℃振荡(200r/min)培养108h,纯多糖产量可达23.2g/L。
短句来源
     The germ culturing condition was as follows: the inoculums was grown in 50 ml seed medium in 500 ml Erlenmeyer flasks at 30℃ for 30 h on a rotary shaker (220 r/min).
     确定的种子培养条件为:初始pH值7.5,装液量50mL/500mL,温度30℃,转速220 r/min,种龄30h。
短句来源
     The best growing temprature for the two srrains was about 30℃. Each strain had its own highest density in certain medium, on rotary shaker in YB medium, thehighest densities of B88 and B237 were 6.1×108·mL-1 and 1.54×108·mL-1, respectively.
     B88和B237菌株最适生长温度为30℃,在YB培养基中生长的最高浓度:B88为6.1×108个·mL-1; B237为1.54×108个·mL-1。
短句来源
     The indicator strain was cultivated at 29 on a rotary shaker at 170r/min for 48h until the concentration was 108/mL.
     指示菌于29℃、170r/min条件下培养48h使指示菌液浓度达10~8个/mL。
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  相似匹配句对
     Development of rotary drum shale shaker
     旋转滚筒振动筛的研制
短句来源
     The optimum fermentation temperature and agitation speed of rotary shaker were found by monofactorial experiment.
     最后通过单因子试验确定最适发酵温度和摇床转速。
短句来源
     Rotary Broaching Technology
     回转拉削工艺
短句来源
     Rotary Curved Screen
     回转形弧形筛
短句来源
     Performance Study of the Shaker Mixers
     摆动式混合机性能研究
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  rotary shaker
The highest yield of protoplasts in the strainAspergillus niger K 10 was obtained from young, freshly harvested hyphae, grown on simple medium of sucrose-asparagine type on a rotary shaker.
      
When the operations were conducted in Erlenmeyer flasks with a volume liquid/volume flask of 0.150 in a rotary shaker at 250 rev/min and 2.5 cm eccentricity, the oxygen absorption rate was 355 ml O2/l/h.
      
The flasks were incubated on a rotary shaker in the dark at 30 °C.
      
The somatic embryos obtained in the bioreactor with 50% DO showed similar behavior to the somatic embryos obtained in the rotary shaker.
      
Secondary multiplication of somatic embryos was achieved in liquid media in rotary shaker and in bioreactors.
      
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Abstract A new species of Tolypocladium was isolated from the scleoderma of Cordyceps sinensis (Berk) Sacc. Which were collected freshly from Dijing Tibeten Austonompus Prefecture, Yunan Provice, China and named as Tolypocladium sinensis Li sp. nov. The morphological characteristic and biological property of new species was studied in several solid mediums. It forms phalide and phialospores in all solid mediums. The base of phalide is spherical or elliptical and terminated by narrow neck bearing globose conidia,...

Abstract A new species of Tolypocladium was isolated from the scleoderma of Cordyceps sinensis (Berk) Sacc. Which were collected freshly from Dijing Tibeten Austonompus Prefecture, Yunan Provice, China and named as Tolypocladium sinensis Li sp. nov. The morphological characteristic and biological property of new species was studied in several solid mediums. It forms phalide and phialospores in all solid mediums. The base of phalide is spherical or elliptical and terminated by narrow neck bearing globose conidia, neck of phalide is slender and curved. Its morphology is different from other species of Tolypocladium, so we consider that Tolypocladium sinensis is a new species. The species of Tolypocladium was not ever be published in China before. When new species was a submerged culture, The medium was composed of peptone, glucose, inorganic salt etc. when it was incubated at 26℃ on rotary shakers for 12 days. its morphology was more various. We tested the antifungal effects of curde extract which is extracted from fermtation broth by acetic ether and is concentrated under pressure. The curde extract showed inhibitory activites. The results of antifungal experiments proved that Tolypocladium sinensis was able to produce cyclosporin.

云南省迪庆藏族自治州产的冬虫夏草经组织分离获得一纯种,在几种琼脂培养基上形成瓶梗及瓶梗孢子。瓶梗基部呈球形或椭园形膨大,瓶颈细长而弯曲,经鉴定隶属于弯颈霉属Tolypocladitum W.Gams,但与弯颈霉属已知种有明显区别,故认为是弯颈霉属一新种,命名为中国弯颈霉Tolypocladilumsinensis Li sp.nov,弯颈霉属的种类在中国从未发现和报导过,本文首次报导。中国弯颈霉经深层培养,培养基为蛋白胨葡萄糖,无机盐类,生长素等,26℃,PH5.2,振速100次/分。发酵周期12天。其分生孢子梗,瓶梗及瓶梗孢子的形态发生很大变异。发酵液经乙酸乙酯提取,减压浓缩,获粗提物,对几种丝状真菌具抑制作用,并与环孢菌素标准样品进行了对照和比较,证实中国弯颈霉能产生环孢菌素。

Abstract A new species of Tolypocladium was isolated from the scleoderma of Cordyceps sinensis (Berk) Sacc. Which were collected freshly from Dijing Tibeten Austonompus Prefecture, Yunan Provice, China and named as Tolypocladium sinensis Li sp. nov. The morphological characteristic and biological property of new species was studied in several solid mediums. It forms phalide and phialospores in all solid mediums. The base of phalide is spherical or elliptical and terminated by narrow neck bearing globose conidia,...

Abstract A new species of Tolypocladium was isolated from the scleoderma of Cordyceps sinensis (Berk) Sacc. Which were collected freshly from Dijing Tibeten Austonompus Prefecture, Yunan Provice, China and named as Tolypocladium sinensis Li sp. nov. The morphological characteristic and biological property of new species was studied in several solid mediums. It forms phalide and phialospores in all solid mediums. The base of phalide is spherical or elliptical and terminated by narrow neck bearing globose conidia, neck of phalide is slender and curved. Its morphology is different from other species of Tolypocladium, so we consider that Tolypocladium sinensis is a new species. The species of Tolypocladium was not ever be published in China before. When new species was a submerged culture, The medium was composed of peptone, glucose, inorganic salt etc. when it was incubated at 26℃ on rotary shakers for 12 days. its morphology was more various. We tested the antifungal effects of curde extract which is extracted from fermtation broth by acetic ether and is concentrated under pressure. The curde extract showed inhibitory activites. The results of antifungal experiments printed that Tolypocladium sinensis was able to produce cyclosporin.

云南省迪庆藏族自治州产的冬虫夏草经组织分离获得一纯种,在几种琼脂培养基上形成瓶梗及瓶梗孢子。瓶梗基部呈球形或椭园形膨大,瓶颈细长而弯曲,经鉴定隶属于弯颈霉属Tolypocladium W.Gams,但与弯颈霉属已知种有明显区别,故认为是弯颈霉属一新种,命名为中国弯颈霉Tolypocladium sinensis Li sp. nov,弯颈霉属的种类在中国从未发现和报导过,本文首次报导。中国弯颈霉经深层培养,培养基为蛋白胨葡萄糖,无机盐类,生长素等,26℃,PH5.2,振速100次/分,发酵周期12天。其分生孢子梗,瓶梗及瓶梗孢子的形态发生很大变异。发酵液经乙酸乙酯提取,减压浓缩,获粗提物,对几种丝状真菌具抑制作用,并与环孢菌素标准样品进行了对照和比较,证实中国弯颈霉能产生环孢菌素。

Calli were initiated from explants of cotyledon and plumular axis of Medicago lupulina on Ayl medium. Two cell lines were obtained from the ealli of cotyledon and plumular axis in liquid Ay2 medium on a rotary shaker at 120 rpm. Both cell lines were able to form calli through monocell cultures in liquid Ay3 or modified K8p medium. After the further growth of calli on solid Ay3 medium they were transferred onto Af medium for the the plantlet regeneration. Regenerated plantlets were cultured, on As medium...

Calli were initiated from explants of cotyledon and plumular axis of Medicago lupulina on Ayl medium. Two cell lines were obtained from the ealli of cotyledon and plumular axis in liquid Ay2 medium on a rotary shaker at 120 rpm. Both cell lines were able to form calli through monocell cultures in liquid Ay3 or modified K8p medium. After the further growth of calli on solid Ay3 medium they were transferred onto Af medium for the the plantlet regeneration. Regenerated plantlets were cultured, on As medium and root formation was induced. Cell lines derived from calli of plumular axis had a higher cell density and regeneration frequency than that derived from calli of cotyledon. In contrast to Ay3 medium, modified K8p medium was more favourable for monocell cultures of Medicago lupulina. Biotin. calcium pantothenate and glucose promoted cell division, callus formation and differentiation. GA3 promoted the development of regenerated buds into plantlets.

采用了改良K8p和Ay3两种培养基,对天蓝苜蓿进行单细胞培养并得到再生植株。来自天蓝苜蓿胚轴愈伤组织的细胞系,在单细胞培养中的愈伤组织分化率明显高于来自子叶愈伤组织的细胞系。改良K8p培养基较之Ay3培养基更利于天蓝苜蓿单细胞培养。生物素、泛酸钙和葡萄糖对细胞系的细胞分裂、愈伤组织诱导和分化有促进作用。赤霉素促进再生幼芽向植株发育。

 
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