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transdifferentiation
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  转分化
    The Effect of TNF-α on TEMT of Renal Interstitial Fibrosis and Inhibition Effect of Chinese Herbs Nourishing Kidney and Activing Blood on Transdifferentiation of Tubular Epithelium
    TNF-α对肾间质纤维化细胞表型变化的作用及补肾活血法中药对肾小管上皮细胞转分化的抑制作用
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    Objective:To investigate the relationship between renal tubular epithelial-myofibroblast transdifferentiation (TEMT) and renal interstitial fibrosis in the rats with chronic aristolochic acid nephropathy (CAAN).
    目的:研究慢性马兜铃酸肾病(CAAN)大鼠肾小管上皮细胞-肌成纤维细胞转分化(TEMT)与肾间质纤维化之间关系。
短句来源
    [Objective] Using transforming growth factor(TGF-β1)to induce the transdifferentiation(EMT) of HK-2 so as to explore the possible mechanism of molecular signal transduction during the EMT process.
    目的用转化生长因子-β1(TGF-β1)诱导人肾小管上皮细胞(HK-2)转分化,探讨转分化过程中可能的细胞信号转导机制。
短句来源
    Conclusions Novel immunomodulator FTY720 can obviously inhibit infiltration of inflammatory cell,transdifferentiation of renal tubule cell,extracellular matrix accumulation and interstitial fibrosis,thus prevent renal disease progression.
    结论FTY720可以明显减轻单侧输尿管梗阻后大鼠肾间质纤维化的程度,减少肾间质炎性细胞浸润,在一定程度上可抑制肾小管上皮细胞转分化和细胞外基质的积聚,对肾功能有一定的保护作用。
短句来源
    Inhibition of Transdifferentiation of Human Renal Tubular Epithelial Cells by Heptocyte Growth Factor
    肝细胞生长因子对人肾小管上皮细胞转分化抑制作用初步研究
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  “transdifferentiation”译为未确定词的双语例句
    Transdifferentiation of tubular epithelial cells in tubulointerstitial fibrosis
    实验性肾小管间质纤维化中肾小管上皮细胞表型转化的研究
短句来源
    Tubular Epithelial-myofibroblast Transdifferentiation and the Expression of Hepatocyte Growth Factor in Renal Tissues of the Rat with Experimental Diabetic Nephropathy
    糖尿病肾病大鼠肾小管上皮细胞转化与肝细胞生长因子的表达
短句来源
    Effects of interleukin-18 on transdifferentiation in renal tubular epithelial cells
    IL-18对肾小管上皮细胞表型转化的影响
短句来源
    Transforming growth factor-β_1(TGF-β_1), an important factor of fibrosis, can promote tubular epithelial cell transdifferentiation towards myofibroblast, enhance the synthesis of ECM proteins and promotes ECM accumulation by increasing the production of protease inhibitors such as plasminogen activator inhibitor-1 (PAI-1) and by decreasing the activity of proteases such as matrix metalloproteinases.
    转化生长因子-β_1(Transforming growth factor-β_1,TGF-β_1)是致纤维化的重要细胞因子。 新近的研究表明,TGF-β_1是通过结缔组织生长因子(Connectiv tissue growth factor,CTGF)促使细胞增生和细胞外基质合成增加进而促进肾小管间质纤维化的进展。
短句来源
    BMP-7 can prevent and inhibit the mRNA expression of TGF-β1 and its typeⅡreceptor, which may be an important mechanism by which BMP-7 inhibit the transdifferentiation of renal tubular epithelial cell.
    RT-PCR显示BMP-7与TGF-β1共同或者预处理HKC细胞熏α-SMAmRNA表达分别为阳?
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  transdifferentiation
Upon transdifferentiation, the cells of spheroids form rods, bipolar cells, and ganglion and glial cells, thus suggesting the possible regenerative potencies of the stem cells in the ciliary body of the mammalian eye.
      
The main event of retinal regeneration in newts is the transdifferentiation of the pigment epithelium cells.
      
Transdifferentiation Potential of Ciliary and Pigment Epithelial Cells in Lower Vertebrates and Mammals
      
Heterogeneous composition of the pigment epithelium in the newt and axolotl reflects high transdifferentiation potential of these regions.
      
After a brief wound healing phase, new tissues and organs develop as a result of extensive cell migration and transdifferentiation.
      
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AIM: To observe the direct effects of peripheral blood monocytes/macrophages (MO/MAC) on renal tubular epithelial cells (RTEC),and further probe into the possible mechanisms. METHODS: Conditioned medium(M-CM) of human peripheral blood MO/MAC was collected and added to HK-2,a human renal proximal tubular cell line.After incubation with M-CM for 24 hours,HK-2 cells were detected for DNA synthesis by -TdR incorporation,osteopontin (OPN) and α-smooth muscle actin (α-SMA) expression by Western blot,and fibronectin(FN)...

AIM: To observe the direct effects of peripheral blood monocytes/macrophages (MO/MAC) on renal tubular epithelial cells (RTEC),and further probe into the possible mechanisms. METHODS: Conditioned medium(M-CM) of human peripheral blood MO/MAC was collected and added to HK-2,a human renal proximal tubular cell line.After incubation with M-CM for 24 hours,HK-2 cells were detected for DNA synthesis by -TdR incorporation,osteopontin (OPN) and α-smooth muscle actin (α-SMA) expression by Western blot,and fibronectin(FN) secretion by ELISA.Furthermore,anti-TGFβ_1 neutralizing antibody and interlukin-10(IL-10) were used separately to antagonize the effects of M-CM on HK-2 cells. RESULTS: ①DNA synthesis,α-SMA expression and FN secretion were all increased in HK-2 cells when incubated with M-CM.②When adding with anti-TGFβ_1 neutralizing antibody (5 mg/L) in the M-CM,the degree of upregulation of α-SMA and FN in HK-2 cells was much lower than that stimulated by M-CM alone.③M-CM added with IL-10 (20 μg/L) had a weaker ability to induce the increasing in α-SMA expression and FN excretion in HK-2 cells, compared with M-CM itself alone.M-CM from MO/MAC preincubated with IL-10 caused a lower upregulation of α-SMA expression in HK-2 cells than M-CM from non-preincubated MO/MAC. CONCLUSION: MO/MAC can directly induce proliferation,transdifferentiation and extracellular matrix secretion in RTEC.TGFβ_1 and proinflammatory cytokines secreted by MO/MAC might be involved in the aboveeffects.

目的 :观察外周血单核巨噬细胞 (MO/MAC)条件培养基 (M -CM)对肾小管上皮细胞 (RTEC)直接作用的生物学效应并探讨可能的作用机制。方法 :应用正常人外周血M -CM刺激人近端肾小管上皮细胞 (HK - 2 ) ,以[3 H]-TdR掺入法检测HK - 2细胞DNA合成、Westernblot法检测骨桥蛋白 (OPN)和α -平滑肌肌动蛋白 (α -SMA)表达、间接抑制ELISA法检测纤连蛋白 (FN)分泌。进一步采用白细胞介素 - 10 (IL - 10 )和转化生长因子 - β1(TGF - β1)中和抗体进行拮抗。结果 :①M -CM可促进HK - 2细胞DNA合成、α -SMA表达及FN分泌。②TGF - β1中和抗体(5mg/L)与M -CM同时作用于HK - 2细胞 ,其α -SMA表达和FN分泌均明显低于M -CM单独作用组。③IL - 10(2 0 μg/L)与M -CM同时作用组的HK - 2细胞 ,α -SMA表达和FN分泌亦明显低于M -CM组 ;IL - 10预孵育MO/MAC组 ,HK - 2细胞α -SMA表达也明显低于M -CM组。结论 :单核巨噬细胞可直接诱导肾...

目的 :观察外周血单核巨噬细胞 (MO/MAC)条件培养基 (M -CM)对肾小管上皮细胞 (RTEC)直接作用的生物学效应并探讨可能的作用机制。方法 :应用正常人外周血M -CM刺激人近端肾小管上皮细胞 (HK - 2 ) ,以[3 H]-TdR掺入法检测HK - 2细胞DNA合成、Westernblot法检测骨桥蛋白 (OPN)和α -平滑肌肌动蛋白 (α -SMA)表达、间接抑制ELISA法检测纤连蛋白 (FN)分泌。进一步采用白细胞介素 - 10 (IL - 10 )和转化生长因子 - β1(TGF - β1)中和抗体进行拮抗。结果 :①M -CM可促进HK - 2细胞DNA合成、α -SMA表达及FN分泌。②TGF - β1中和抗体(5mg/L)与M -CM同时作用于HK - 2细胞 ,其α -SMA表达和FN分泌均明显低于M -CM单独作用组。③IL - 10(2 0 μg/L)与M -CM同时作用组的HK - 2细胞 ,α -SMA表达和FN分泌亦明显低于M -CM组 ;IL - 10预孵育MO/MAC组 ,HK - 2细胞α -SMA表达也明显低于M -CM组。结论 :单核巨噬细胞可直接诱导肾小管上皮细胞增殖、表型转化以及分泌细胞外基质增加 ;其分泌的TGF - β1及炎性细胞因子可能参与介导上述效应。

Objective To investigate the effects of urinary protein on activation and transdifferen-tiation of proximal tubular cells (PTC), and study the related mechanism. Methods Urinary proteins were abstracted by fractional precipitation method with ammonium sulfate from nephrotic proteinuria of three patients with minimal change disease (MCD) . Cultured HK-2 cells, a human PTC line, were used for this study. Cell proliferation rate was assayed by 3H-TdR incorporation. The level of secretary fibronectin(FN) was detected...

Objective To investigate the effects of urinary protein on activation and transdifferen-tiation of proximal tubular cells (PTC), and study the related mechanism. Methods Urinary proteins were abstracted by fractional precipitation method with ammonium sulfate from nephrotic proteinuria of three patients with minimal change disease (MCD) . Cultured HK-2 cells, a human PTC line, were used for this study. Cell proliferation rate was assayed by 3H-TdR incorporation. The level of secretary fibronectin(FN) was detected by ELBA. Western blot assay was used for detecting the cellularα-SMA, MCP-1 proteins synthesis, and RT-PCR for TGF-P mRNA expression respectively. Results (1)The main components of abstracted urinary protein were albumin(84.5% ), transferring(8.0% ) and IgG(1. 2% ) . (2) Urinary protein, at concentration from 0.2 mg/ml to 10 mg/ml, enhenced 3H-TdR incorporation of HK-2 cells from 3. 33 to 8. 59 folds as compared to control group in a concentration dependent manner ( P < 0. 01 ~ 0. 001). (3) Secretary FN level was increased when incubated with 0. 5- 5mg/ml urinary protein ( P < 0. 001). (4) Urinary protein also induced the synthesis of MCP-1 and α>SMAproteins, as well as the up-regulating TGF-P mRNA expression in HK-2 cells. (5) Urinary protein-inducedMCP-1 protein synthesis and FN secretion were partly attenuated by anti-TGF-β1 neutralizing antibody.Conclusions Urinary protein can activate PTC through stimulating cells proliferation, ECM secretion and chemoattractants and growth factors synthesis, and also induce PTC transdifferentiation by expressing A-SMAproteins. TGF-β intervenes in the stimulating effects of urinary proteins on ECM and MCP-1 protein synthesisof PTC. Those may play important roles on the proteinuria induced tubulointerstitial damage during progression of chronic glomerular disease.

目的 探讨尿蛋白对肾小管损伤的途径及加重肾脏病变的相关机制。方法 用硫酸铵沉淀法提取肾小球微小病变患者尿液中的总蛋白成分。体外培养人近端肾小管上皮细胞系HK-2细胞。用3H-TdR掺入法测定细胞增殖水平,Western blot方法检测细胞合成单核细胞趋化因子(MCP-1)及α-平滑肌肌动蛋白(α-SMA)水平,RT、PCR检测细胞TGF-βmRNA表达,间接竞争ELISA检测细胞上清的纤连蛋白(FN)水平。结果(1)提取的尿蛋白主要成分为白蛋白、转铁蛋白和IgG,分别是84.5%、8.0%和1.2%。(2)0.2~10mg/ml浓度的尿蛋白能刺激HK-2细胞增殖,为对照组的3.33~8.59倍(P<0.01~0.001)。(3)在0.5~5mg/ml浓度的尿蛋白作用下,HK-2细胞分泌FN也明显高于对照组(P<0.001),5mg/ml时的作用最强,为对照组的(2.908±0.544)倍。(4)尿蛋白还诱导HK-2细胞合成MCP-1、α-SMA蛋白及上调表达TGF-β mRNA,当浓度为5~10 mg/ml时其作用最强。(5)TGF-β1中和抗体在浓度为1~2μg/ml时可部分阻断尿蛋白(5mg/ml)对H...

目的 探讨尿蛋白对肾小管损伤的途径及加重肾脏病变的相关机制。方法 用硫酸铵沉淀法提取肾小球微小病变患者尿液中的总蛋白成分。体外培养人近端肾小管上皮细胞系HK-2细胞。用3H-TdR掺入法测定细胞增殖水平,Western blot方法检测细胞合成单核细胞趋化因子(MCP-1)及α-平滑肌肌动蛋白(α-SMA)水平,RT、PCR检测细胞TGF-βmRNA表达,间接竞争ELISA检测细胞上清的纤连蛋白(FN)水平。结果(1)提取的尿蛋白主要成分为白蛋白、转铁蛋白和IgG,分别是84.5%、8.0%和1.2%。(2)0.2~10mg/ml浓度的尿蛋白能刺激HK-2细胞增殖,为对照组的3.33~8.59倍(P<0.01~0.001)。(3)在0.5~5mg/ml浓度的尿蛋白作用下,HK-2细胞分泌FN也明显高于对照组(P<0.001),5mg/ml时的作用最强,为对照组的(2.908±0.544)倍。(4)尿蛋白还诱导HK-2细胞合成MCP-1、α-SMA蛋白及上调表达TGF-β mRNA,当浓度为5~10 mg/ml时其作用最强。(5)TGF-β1中和抗体在浓度为1~2μg/ml时可部分阻断尿蛋白(5mg/ml)对HK-2细胞FN分泌和MCP-1蛋白合成的刺激作用。结论 人类微小病变来源的尿蛋白可刺激人近端肾小管上皮细胞增殖、分泌细胞外基质蛋白、合成炎症趋化因子,诱导肾小管细胞发生以α-SMA为标志物的表型转化。这一效应部分通过TGF-β1的介导作用。这可?

Objective To examine whether there is a synergism of transforming grouth factor β1 (TGF β1) and epidermal grouth factor (EGF) in inducing transdifferentiation of human tubular epithelial cell line (HKC) Methods HKC cells were divided into different groups: (1) serum free(negative control); (2) EGF (10, 20, 40 ng/ml) treated; (3) TGF β1 (3, 10, 20 ng/ml) treated; (4) EGF (10, 20, 40 ng/ml) plus TGF β1 (3, 10, 20 ng/ml) treated The cells were typically incubated for 48 hours after addition of...

Objective To examine whether there is a synergism of transforming grouth factor β1 (TGF β1) and epidermal grouth factor (EGF) in inducing transdifferentiation of human tubular epithelial cell line (HKC) Methods HKC cells were divided into different groups: (1) serum free(negative control); (2) EGF (10, 20, 40 ng/ml) treated; (3) TGF β1 (3, 10, 20 ng/ml) treated; (4) EGF (10, 20, 40 ng/ml) plus TGF β1 (3, 10, 20 ng/ml) treated The cells were typically incubated for 48 hours after addition of cytokines HKC was detected by light microscopy and electron microscopy. The expression of vimentin and cytokeratin were assessed by indirect immunoflourescence, the expressions of E cadherin and α smooth muscle actin (α SMA) of HKC cells were assessed by indirect immunohistochemical double staining. The persentages of α SMA(+) HKC cells were assessed by flow cytometry Results EGF at 10, 20, 40 ng/ml or TGF β1 at 3 ng/ml did not induce visible transdifferentiation of HKC TGF β1 at 10, 20 ng/ml induced light transdifferentiation of HKC There was a synergistic effect of EGF (10, 20, 40 ng/ml) and TGF β1 (3, 10, 20 ng/ml) in induction of transdifferentiation of HKC into myofibroblasts [(35 17±2 86)%,(51 2±1 10)%,(55 43±1 15)% vs (3 53±0 09)%, P <0 05] Conclusion There is a synergism of EGF and TGF β1 at the given concentrations on transdifferentiation of HKC into myofibroblasts

目的 探讨表皮生长因子 (EGF)和转化生长因子 β1(TGF β1)及两者联合应用对人类肾小管上皮细胞 (HKC)转分化的影响。方法 用相差显微镜、透射电镜和扫描电镜观察HKC株的形态和结构。应用间接免疫荧光法检测HKC角蛋白和波形蛋白的表达 ;应用免疫组织化学双染色技术测定HKCE 钙粘连蛋白和α 平滑肌肌动蛋白 (α SMA)的表达。应用流式细胞技术测定HKC表达α SMA阳性的百分率。结果  10、2 0、4 0ng/ml浓度EGF及 3ng/ml浓度TGF β1单独作用时 ,无明显促进HKC转分化的作用。TGF β1单独应用 (10、2 0ng/ml)有轻度的诱导HKC转分化的作用。当TGF β1(3ng/ml)与EGF(10ng/ml)、TGF β1(10ng/ml)与EGF(2 0ng/ml)、TGF β1(2 0ng/ml)与EGF(40ng/ml)共同作用时 ,对诱导HKC转分化有显著的协同作用 [(35 17± 2 86 ) %、(5 1 2± 1 10 ) %、(5 5 4 3± 1 15 ) %vs(3 5 3± 0 0 9) % ,P <0 0 5 ]。结论 一定浓度的T...

目的 探讨表皮生长因子 (EGF)和转化生长因子 β1(TGF β1)及两者联合应用对人类肾小管上皮细胞 (HKC)转分化的影响。方法 用相差显微镜、透射电镜和扫描电镜观察HKC株的形态和结构。应用间接免疫荧光法检测HKC角蛋白和波形蛋白的表达 ;应用免疫组织化学双染色技术测定HKCE 钙粘连蛋白和α 平滑肌肌动蛋白 (α SMA)的表达。应用流式细胞技术测定HKC表达α SMA阳性的百分率。结果  10、2 0、4 0ng/ml浓度EGF及 3ng/ml浓度TGF β1单独作用时 ,无明显促进HKC转分化的作用。TGF β1单独应用 (10、2 0ng/ml)有轻度的诱导HKC转分化的作用。当TGF β1(3ng/ml)与EGF(10ng/ml)、TGF β1(10ng/ml)与EGF(2 0ng/ml)、TGF β1(2 0ng/ml)与EGF(40ng/ml)共同作用时 ,对诱导HKC转分化有显著的协同作用 [(35 17± 2 86 ) %、(5 1 2± 1 10 ) %、(5 5 4 3± 1 15 ) %vs(3 5 3± 0 0 9) % ,P <0 0 5 ]。结论 一定浓度的TGF β1和EGF在诱导HKC转分化中有显著的协同作用 ,为研究HKC转分化提供了模型。

 
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