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unit of enzyme
相关语句
  酶单位
     A active unit of enzyme means the level of enzyme in one milliliter working solution which has 50% inhibition rate of pyrogallol self-oxidation in one minute at 25℃, pH8.2 and at 325 nm UV. The activity of SOD in RBC of the students was 533.48±83.81 units/ml RBC.
     1个酶单位是指在25℃、pH8.2和325nm紫外光分光光度法测定时,每ml反应液中每分钟抑制邻苯三酚自氧化速率达50%的酶量。
短句来源
  “unit of enzyme”译为未确定词的双语例句
     The enzyme was extracted with phosphate buffer ,one unit of enzyme activity was defined as the amount of enzyme catalyzing reduction 1μmol NADP+ per minute under the assay conditions. The purification procedure of alcohol dehydrogenase I involved alcohol precipitation and followed by ion-exchange chromatography on DEAE-sepharose, gel filter chromatography on Sephadex G-75 , and affinity chromatography on Blue sepharose Fast flow.
     以为每分钟催化还原1μmolNADP+的活力定义为酶活力单位,收集活性分部,细胞破碎后所得粗酶经乙醇分级沉淀(30-60%),层析柱分离,包括离子交换层析、凝胶过滤层析和亲和层析。
短句来源
     One unit of enzyme activity was defined as the amount of enzyme catalyzing oxydation 1μmol NADH per minute under the assay conditions. The subunit of enzyme was estimated to 40KD on SDS-PAGE.
     凝胶过滤层析后所得酶活力分部进行SDS-PAGE电泳,显示为一条蛋白带,得到电泳纯的醇脱氢酶II,亚基分子量为40kD。
短句来源
     One unit of enzyme activity represented the formation of 1 nmol of methyl-MP per hour of incubation, per milliliter of packed red cells at 37℃.
     TPMT的活性用37℃时每小时每毫升红细胞生成多少纳摩尔的6-MeMP来表示。
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  相似匹配句对
     Stroke Unit
     卒中单元
短句来源
     On the Unit of Translation
     翻译单位论
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     On the Unit Crime
     单位犯罪的法律思考
短句来源
     On Unit Criminal Offence
     论单位犯罪
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     Enzyme technology
     酶技术将废料变为饲料
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  unit of enzyme
Changes in homospecific activity (unit of enzyme activity per unit of enzyme protein; Rush, Kindler and Udenfriend, 1974.
      
The partially purified inactive enzyme releases 0.048 nmol of HCN per unit of enzyme activated.
      
Since MnP decolorized amaranth about 30 times more rapidly than laccase per unit of enzyme activity, MnP should have contributed more to decoloration than laccase in batch cultures.
      
Since, however, the results obtained in different research centres are almost completely incomparable, an attempt has been made to introduce a uniform system for expressing a unit of enzyme activity.
      
The unit of enzyme activity is defined as the oxidation of 1 pmole of DPNH per min.
      
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Activity of superoxide dismutase ( SOD ) in RBC from 117 healthy university students was measured with the method of modified pyro-gallol self-oxidation. A active unit of enzyme means the level of enzyme in one milliliter working solution which has 50% inhibition rate of pyrogallol self-oxidation in one minute at 25℃, pH8.2 and at 325 nm UV. The activity of SOD in RBC of the students was 533.48±83.81 units/ml RBC. There was no significant difference between men and women. In this study, the...

Activity of superoxide dismutase ( SOD ) in RBC from 117 healthy university students was measured with the method of modified pyro-gallol self-oxidation. A active unit of enzyme means the level of enzyme in one milliliter working solution which has 50% inhibition rate of pyrogallol self-oxidation in one minute at 25℃, pH8.2 and at 325 nm UV. The activity of SOD in RBC of the students was 533.48±83.81 units/ml RBC. There was no significant difference between men and women. In this study, the variation in group and between groups were 3.14 and 5.27%, respectively. It is believed that the method is simple and more accurate when the activity of SOD in RBC is expressed by the active unit of SOD in every milliliter RBC.

用改良的邻苯三酚自氧化法检测了117例健康大学生红细胞超氧化物歧化酶(SOD)活性。1个酶单位是指在25℃、pH8.2和325nm紫外光分光光度法测定时,每ml反应液中每分钟抑制邻苯三酚自氧化速率达50%的酶量。健康大学生红细胞SOD活性为533.48±83.81单位/ml红细胞,无显著的性别差异。本法批内及批间变异分别为3.14%和5.27%。作者认为以每ml红细胞中SOD活力单位表示较为简便、正确。

By using BioRex70 ionexchange, electrophoresis through denaturing polyacrylamide gels, activity assay by PCR and other simple methods, purification and activity assay, expressed in E. Coli DH5α, have been accomplished within 30 hours. It concludes that 30 000 units of enzyme are purified from 2 000 ml of fermenting liquor. The resulting enzyme Taq DNA polymerase makes no difference with the Promega in thermostability. Further study is needed in its extended specificities.

采用聚乙烯亚胺沉淀、BioRex70离子交换、变性聚丙烯酰胺凝胶电泳、PCR检测等简单方法,在30h内完成TaqDNA聚合酶的基因重组质粒在工程菌DH5α中超表达酶的提取纯化及酶活标定。2000ml发酵液可提纯3万U的TaqDNA聚合酶,与国外优质产品(promega)相比,热稳定性没有差别,扩增特异性尚需进一步探讨。

Response Surface Analysis(RSA)was used to study the technology for the preparation of with humecants with hydrolyzing wheat-embryo with enzyme.The antioxidant activity of the sausages added with humecants was determined.The results revealed that the optimum production condition could be obtained by treating 400 ml 10% wheat-embryo milk with 860 units of enzyme per gram wheat-embryo for 4.6 hours under 44.4℃.The water activity and the degree of hydrolysis of the humectants under these conditions were...

Response Surface Analysis(RSA)was used to study the technology for the preparation of with humecants with hydrolyzing wheat-embryo with enzyme.The antioxidant activity of the sausages added with humecants was determined.The results revealed that the optimum production condition could be obtained by treating 400 ml 10% wheat-embryo milk with 860 units of enzyme per gram wheat-embryo for 4.6 hours under 44.4℃.The water activity and the degree of hydrolysis of the humectants under these conditions were 0.896 and 26.5% respectively.The shelf-life of the sausages added with humectants extended significantly.

采用响应面分析方法研究了酶法水解麦胚制取持水剂的生产工艺,并对产品应用于香肠中的抗氧化效果进行了试验。试验结果表明:酶法水解麦胚制取持水剂的优化工艺参数为:对10%的麦胚乳400ml,反应温度为44.4℃、反应时间为4.6h、酶与底物浓度比为860μ/g。经优化工艺生产的持水剂的水分活度为0.896,所对应的水解度为26.5%。应用于香肠可显著提高其货架寿命。

 
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