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capillary transfer
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  “capillary transfer”译为未确定词的双语例句
     The Application of Downward Capillary Transfer in the Southern Blotting of HBV
     向下转膜法在乙肝病毒Southern杂交中的应用
短句来源
     Objective To investigate the effect of downward capillary transfer in the Southern blotting of HBV. Methods Transfecting the plasmid which contains 1.3 fold overlength genome of HBV to HepG2 cells so as to get the expression of HBV, extracting total genome DNA of cells by common protocol and loading on agarose gel electrophoresis, transfering the DNA of the agarose gel to Nylon membranes by both the downward and upward capillary transfer;
     目的评价向下转膜法在乙肝病毒(HBV)Southern杂交中的应用效果。 方法用带有1.3倍HBV基因的真核表达载体转染HepG2细胞,使其产生瞬时HBV表达,72小时收集细胞并提取细胞总DNA,琼脂糖凝胶电泳后,以向上和向下两种方式转移在尼龙膜上;
短句来源
     conversely,25μg of total DNA of cells can only be detected low DNA expression of HBV in upward capillary transfer.
     而向上转膜所需的总DNA量大,灵敏度低,25μg的细胞总DNA电泳后转膜,仅能检测出弱的条带。
短句来源
     Conclusion The downward capillary transfer is better than that of upward in the Southern blotting of HBV, and should be applied broadly.
     结论向下转膜法是一种较好的转膜方式,适于对乙肝病毒的Southern杂交,值得推广应用。
短句来源
     The DNAs in the agarose gel then were transfered to positively charged nylon membrane by capillary transfer.
     用毛细转移法将凝胶上的DNA转移至正电荷尼龙膜上。
短句来源
  相似匹配句对
     Research on Transfer Characteristics of Solutes in Capillary Electrochromatography
     毛细管电色谱中溶质的输运特征研究
短句来源
     ANALYSIS OF EVAPORATION HEAT TRANSFER IN CAPILLARY TUBES
     毛细管内蒸发传热机理的分析
短句来源
     On Pragmatic Transfer
     语用迁移性之探讨
短句来源
     Syntactic Transfer
     句法迁移
短句来源
     Capillary Hemangioblastoma
     血管母细胞瘤
短句来源
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  capillary transfer
Samples were either blotted manually, or by alkaline capillary transfer using 100 mM NaOH.
      
First, by the capillary transfer of water and salt from the ground, when the stones are located near the sea.
      
This homogeneous network allowed good capillary transfer for long distances (1-2?m).
      
Its porous network was organized into two subnetworks of rectilinear and sinuous cracks, which limited capillary transfer to ~10?cm.
      
Virtual Northern blots were prepared by running 500 ng of amplicon cDNA in 1.5% agarose gels and blotting using standard capillary transfer.
      
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Objective To investigate the effect of downward capillary transfer in the Southern blotting of HBV. Methods Transfecting the plasmid which contains 1.3 fold overlength genome of HBV to HepG2 cells so as to get the expression of HBV, extracting total genome DNA of cells by common protocol and loading on agarose gel electrophoresis, transfering the DNA of the agarose gel to Nylon membranes by both the downward and upward capillary transfer;labelling HBV DNA probe with the Gene Image Alkphos Direct...

Objective To investigate the effect of downward capillary transfer in the Southern blotting of HBV. Methods Transfecting the plasmid which contains 1.3 fold overlength genome of HBV to HepG2 cells so as to get the expression of HBV, extracting total genome DNA of cells by common protocol and loading on agarose gel electrophoresis, transfering the DNA of the agarose gel to Nylon membranes by both the downward and upward capillary transfer;labelling HBV DNA probe with the Gene Image Alkphos Direct labelling system and hybridizing with the total DNA of cells on the Nylon membranes, comparing the sensitivity of upward with downward capillary transfers in the Southern blotting of HBV. Results We found that the sensitivity of downward capillary transfer is higher than that of upward in the Southern blotting of HBV, 0.5μg of total DNA of cells can be detected the DNA expression of HBV, there are only 8 percent of total DNA left in the agarose gel after transferred by downward capillary transfer;conversely,25μg of total DNA of cells can only be detected low DNA expression of HBV in upward capillary transfer. Conclusion The downward capillary transfer is better than that of upward in the Southern blotting of HBV, and should be applied broadly.

目的评价向下转膜法在乙肝病毒(HBV)Southern杂交中的应用效果。方法用带有1.3倍HBV基因的真核表达载体转染HepG2细胞,使其产生瞬时HBV表达,72小时收集细胞并提取细胞总DNA,琼脂糖凝胶电泳后,以向上和向下两种方式转移在尼龙膜上;用化学发光标记和检测试剂盒将乙肝病毒基因片段标记成探针,再与向上和向下转膜后的尼龙膜进行杂交,判断产生明显HBV杂交条带所需的细胞总DNA量,从而验证向下转膜法在乙肝病毒Southern杂交中的应用效果。结果发现向下转膜后,检测HBV表达所需的细胞总DNA量极少,灵敏度高,0.5μg细胞总DNA电泳后转膜,即可检测出HBVDNA的表达,凝胶中仅有8%的DNA残留;而向上转膜所需的总DNA量大,灵敏度低,25μg的细胞总DNA电泳后转膜,仅能检测出弱的条带。结论向下转膜法是一种较好的转膜方式,适于对乙肝病毒的Southern杂交,值得推广应用。

Objective:To study the change of mRNA expreesion of connexin 40(Cx40) and connexin 43(Cx43) in human atrial myocardium from valvular permanent atrial fibrillation(Af). Method:Eleven cases of human right atrial appendage(RAA) samples were collected from rheumatic heart disease during valve replacement, 8 of that were Af samples that average sustained Af period were (~5.6 ±6.5) years, 3 of that remained sinus rhenium(SR), 4 of that were male, 7 of that were female, average (50.8±13.7) years of age, average Ⅱ~Ⅲ...

Objective:To study the change of mRNA expreesion of connexin 40(Cx40) and connexin 43(Cx43) in human atrial myocardium from valvular permanent atrial fibrillation(Af). Method:Eleven cases of human right atrial appendage(RAA) samples were collected from rheumatic heart disease during valve replacement, 8 of that were Af samples that average sustained Af period were (~5.6 ±6.5) years, 3 of that remained sinus rhenium(SR), 4 of that were male, 7 of that were female, average (50.8±13.7) years of age, average Ⅱ~Ⅲ cardiac function class. Total RNA was purified, equal amounts of RNA (11 μg/lane) from each sample were run on formaldehyde-agarose gels and capillary-transferred onto nylon membrane, the resulting membrane was hybridized with radiolabelled Cx43 and Cx40 cDNA probe, respectively. The hybridized membrane was exposed to X-OMAT film. Quantification of Northern blots was carried out by densitometric scanning of the autoradiograms. To correct possible differences in the gel loading, hybridization with β-actin was performed. All the values were expressed as a percentage of the densitometric value of each sample on β-actin. Result:The mRNA levels of Cx43 and Cx40 were no statistically significant differences between Af and SR samples. Conclusion:mRNA expression level of Cx40 and Cx43 remain stable in human atrial myocardium during permanent valvular Af.

目的测定风湿性心脏瓣膜病(风心病)心房颤动(Af)心房肌缝隙连接蛋白(Connexin)40(Cx40)和Connexin43(Cx43)mRNA表达水平的变化。方法11例风心病患者[Af8例,窦性心律3例;男4例,女7例;平均年龄(50.8±13.7)岁;平均Af时间(5.6±6.5)年]的右心耳心肌1小块,提取所有标本的总RNA,等量转移至尼龙膜上,用PCR合成的特异cDNA探针,标记上放射性同位素(α32PdCTP)后与尼龙膜杂交,然后将杂交膜与X光胶片行放射自显影,在灰度扫描仪下得到每例标本mRNA水平的灰度值,为了进一步矫正转膜时可能的定量不统一,将同1块杂交膜先后与Cx40探针、Cx43探针和内参照βactin杂交,以Cx40/βactin和Cx43/βactin的百分比作为统计变量进行分析。结果在Af与窦性心律标本中,Cx40和Cx43的mRNA表达量未发生明显改变(P>0.05)。结论在风心病Af患者中,未发现缝隙连接蛋白Cx40和Cx43的mRNA表达量明显改变。

Objective: To study the mitochondrial deletion DNAs of skeletal muscle from patients with chronic progressive external ophthalmoplegia(CPEO) and Kearns-Sayre syndrome.Methods: DNA samples extracted from skeletal muscles of 11 patients with CPEO and Kearns-Sayre syndrome were digested with the restriction endonuclease(PvuⅡ).DNA fragments were separated by standard protocols of an agarose gel electrophoresis.The DNAs in the agarose gel then were transfered to positively charged nylon membrane by capillary transfer.Total...

Objective: To study the mitochondrial deletion DNAs of skeletal muscle from patients with chronic progressive external ophthalmoplegia(CPEO) and Kearns-Sayre syndrome.Methods: DNA samples extracted from skeletal muscles of 11 patients with CPEO and Kearns-Sayre syndrome were digested with the restriction endonuclease(PvuⅡ).DNA fragments were separated by standard protocols of an agarose gel electrophoresis.The DNAs in the agarose gel then were transfered to positively charged nylon membrane by capillary transfer.Total mitochondrial DNA was extracted from normal people′s blood as probes,which were labeled with digoxigenin-dUTP.The positively charged nylon membranes were studied by prehybridization,hybridization,post-hybridization washes,and immunological detection.Results: One normal and one abnormal bands,corresponding to single mitochondrial DNA deletion were presented in 7 patients.The proportion of deleted fragments ranged from 50%~75%.The deletions ranged in size from 4.5~5.5 kb.Conclusion: We found a high frequency of mitochondrial DNA deletions in Chinese patients with CPEO and KSS.Thus,the mitochondrial DNA deletion is considered to be closely related with CPEO and KSS.

目的:检测慢性进行性眼外肌麻痹(chronic progressive external ophthalmoplegia,CPEO)及Kearns-Sayre综合征(KSS)患者骨骼肌细胞线粒体DNA的缺失情况。方法:从11例CPEO和KSS患者的骨骼肌活检标本中,提取总体DNA。限制性内切酶PvuⅡ消化1 h,将消化后的DNA片段进行琼脂糖凝胶电泳,使DNA片段按大小分离。用毛细转移法将凝胶上的DNA转移至正电荷尼龙膜上。从正常人全血中提取全长线粒体DNA作为探针,进行地高辛-dUTP标记,并与正电荷尼龙膜进行预杂交、杂交、杂交后冲洗及显色反应。结果:11例中,7例除有一条正常大小杂交带外,还有一条异常的缺失型线粒体DNA杂交带。剂量分析表明,缺失型线粒体DNA占总线粒体DNA的50%~75%。缺失片断在4.5~5.5 kb之间。结论:CPEO及KSS患者骨骼肌线粒体DNA缺失率较高,线粒体DNA基因缺失与线粒体疾病密切相关。

 
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