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hybrid antibody
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  “hybrid antibody”译为未确定词的双语例句
     Methods Firstly, the Fd gene of a murine anti TNF α antibody was paired with a repertoire of human κ chain, and displayed on the filamentous phage forming a hybrid phage antibody library. After four round panning against TNF α, the hybrid antibody fragments containing human κ chain were obtained.
     方法首先用鼠单抗Fd段基因与人的κ链基因库相互配对,构建成人-鼠杂合噬菌体抗体库,筛选可与TNF-α结合的克隆,所获得的人κ链基因再与人Fd基因库组合,建立人源噬菌体抗体库,再进行筛选;
短句来源
     The size of the library, antibody gene recombinant percentage and diversity were identified by colony counting, plasmid digestion and colony sequence analysis, respectively. Purified γ-sm was used as antigen to screen the displayed phage hybrid antibody library rescued by helper phage M13K07 for three rounds.
     通过稀释滴定、限制性酶切和随机测序,对所建杂合库的库容、重组率和多样性分别进行鉴定,以M13K07辅助噬菌体超感染,利用亲和纯化的γ-sm为抗原,对挽救展示的噬菌体抗体库进行3轮淘筛和克隆鉴定。
短句来源
  相似匹配句对
     Construction of human-mouse hybrid Fab phage antibody libraries
     人-鼠杂合Fab噬菌体抗体库的建立
短句来源
     Hybrid Binders
     混合粘结剂
短句来源
     Hybrid Cell Secreting Monoclonal Antibody Against Arginine Vasopressin
     分泌抗精氨酸加压素单克隆抗体杂交瘤细胞株的建立
短句来源
     Hybrid ATM
     混合的ATM技术
短句来源
     antibody drug
     抗体药物
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  hybrid antibody
The immunoenzymatic assay utilized a simple microtiter plate format and an anti-RNA:DNA hybrid antibody for the detection of NASBA product (predominantly RNA) hybridized to an immobilized DNA probe.
      
Our efficacy results in this study suggest that drug, specifically removed from the circulation by hybrid-hybrid antibody previously located to the tumour mass, can be made available in a pharmacologically active from.
      
The mixed hemadsorption hybrid antibody (MHA.HA) test was applied successfully to the detection of antigens on the surface of testicular cells separated into sub-populations by velocity sedimentation at unit gravity in the "staput" apparatus.
      
Using the highly sensitive method of immunoelectron microscopy with a hybrid antibody, it was found that only 5.5% of cells in the thymus carried the Ly-4.2 specificity, compared with 55.7% in the spleen and 20.9% in lymph nodes.
      
Somatic generation of hybrid antibody H chain genes in transgenic mice via interchromosomal gene conversion.
      


The antibody specific for DNA/RNAhybrid was prepared by immunizing rabbitwith polydT?polyrA complexed with meth-ylated bovine serum albumin according tothe method of Stollar. The distribution of DNA/RNA hybridson polytene chromosomes of Drosophila viri-lis was then studied with immunoperoxidasestaining technique,using rabbit anti-DNA/RNA hybrids antibody.The bands wereintensely stained and found to be occurredin many chromosomal subdivisions,whenthe chromosomes were exposed to denaturingcondition...

The antibody specific for DNA/RNAhybrid was prepared by immunizing rabbitwith polydT?polyrA complexed with meth-ylated bovine serum albumin according tothe method of Stollar. The distribution of DNA/RNA hybridson polytene chromosomes of Drosophila viri-lis was then studied with immunoperoxidasestaining technique,using rabbit anti-DNA/RNA hybrids antibody.The bands wereintensely stained and found to be occurredin many chromosomal subdivisions,whenthe chromosomes were exposed to denaturingcondition and then allowed to reanneal.Without previous denaturation and reanneal-ing,we could hardly detect DNA/RNAhybrids in D.virilis salivary gland chro-mosomes.The immunoperoxidase method did ??not stain polytene chromosomes when theywere treated with RNase before denaturationand reannealing.Similar negative results wereobtained when normal rabbit serum was usedinstead of the DNA?RNA antibody.Thepolytene chromosomes were not stained too,when they were treated with RNase-H 1 afterdenaturation and reannealing. So the antibody is specific against DNA?RNA hybrid molecules after it was absorbedwith polyA and oligodT-cellulose.The pos-sible reasons for the necessity of denatura-tion and reannealing proceedure to demon-strate such hybrids in D.virilis polytenechromosomes are briefly disscussed.

应用兔抗polydT·polyrA的特异性抗体,在果蝇D.virilis多线染色体上用免疫酶标的方法检测到DNA·RNA内源杂交分子。多线染色体制片必须先进行变性复性处理(即内源分子杂交技术)才能得到明显的酶标显色带。大致可分辨到363条带,遍及染色体的各个区域。用DNA·RNA杂交分子为特异性底物的RNA酶H处理后再经变性复性处理后的多线染色体,免疫酶标的阳性带几乎全部消失,因此证明经内源分子杂交技术处理后用免疫化学方法检测到的阳性带确是由DNA·RNA内源杂交分子所构成的。

Objective Humanizing murine anti TNF α antibody Fab fragment with the method of epitope guided selection. Methods Firstly, the Fd gene of a murine anti TNF α antibody was paired with a repertoire of human κ chain, and displayed on the filamentous phage forming a hybrid phage antibody library. After four round panning against TNF α, the hybrid antibody fragments containing human κ chain were obtained. The selected human κ chain genes were in turn paired with a repertoire of human Fd fragments...

Objective Humanizing murine anti TNF α antibody Fab fragment with the method of epitope guided selection. Methods Firstly, the Fd gene of a murine anti TNF α antibody was paired with a repertoire of human κ chain, and displayed on the filamentous phage forming a hybrid phage antibody library. After four round panning against TNF α, the hybrid antibody fragments containing human κ chain were obtained. The selected human κ chain genes were in turn paired with a repertoire of human Fd fragments forming a human phage antibody library, and the phages were selected against TNF α again. The other way, human Fd gene was first selected by pairing human Fd repertoire with mouse anti TNF α κ chain. Then the human Fd gene was paired with selected human κ chains, and their binding activity to TNF was determined. Results Human Fabs obtained by first selecting human κ chains using mouse Fd chain as template showed nonspecific binding to some unrelated proteins. When human Fd chain was selected first and paired with selected human κ chain, the resulted human Fab bound to TNF α specifically, and their human origin was proved. Conclusion The epitope guided selection is thougt to be a valid method for humanizing mouse antibodies.

目的用抗原表位定向选择(epitopeguidedselection)方法,将鼠源性抗TNF-α单抗Fab段改造成完全人源性Fab。方法首先用鼠单抗Fd段基因与人的κ链基因库相互配对,构建成人-鼠杂合噬菌体抗体库,筛选可与TNF-α结合的克隆,所获得的人κ链基因再与人Fd基因库组合,建立人源噬菌体抗体库,再进行筛选;类似地以鼠κ链基因和人Fd库组合构建杂合抗体库,筛选人Fd基因,再和筛到的人κ链配对,选择与TNF-α特异结合的克隆。结果以鼠抗体Fd段定向选择人κ链获得能与TNF-α特异结合的杂合抗体Fab片段,再以这些人κ链定向选择人Fd段获得能与TNF-α结合的完全人源性的Fab,但这些Fab除与TNF-α反应外,与一些无关蛋白有交叉反应。以鼠单抗κ链定向选择人Fd段获得能与TNF-α特异结合的杂合Fab,其中一个克隆显示很强的结合活性,以该克隆的Fd与筛到的人κ链配对,得到了能与TNF-α特异结合的人源性Fab片段。结论抗原表位定向选择方法提供了一种有效的鼠单抗人源化路线

There were no definite conclusions about transcription sites of rRNA genes in nucleoli of eukary-otes for a long time. We used the wheat as specimen and observed ultrastructure of chromatin in FC of nucleoli by conventional transmission electron microscope. And we employed anti-DNA antibodies to analyze the distribution of DNA in nucleoli, and found that DNA located in FC, DFC and the transitional region between them. Using anti-UBF antibodies to study localization of RNA polymerase I transcription...

There were no definite conclusions about transcription sites of rRNA genes in nucleoli of eukary-otes for a long time. We used the wheat as specimen and observed ultrastructure of chromatin in FC of nucleoli by conventional transmission electron microscope. And we employed anti-DNA antibodies to analyze the distribution of DNA in nucleoli, and found that DNA located in FC, DFC and the transitional region between them. Using anti-UBF antibodies to study localization of RNA polymerase I transcription factor UBF in nucleoli, we observed that UBF located in DFC and the transitional region between DFC and FC in wheat, but not in FC. Furthermore, with anti-RNA/DNA hybrid antibodies, we directly and selectively labeled the transcription sites of rRNA genes of nucleoli and revealed that it located in DFC and the transitional region between DFC and FC in wheat.

真核细胞核仁中rRNA基因转录位点是长期以来未能解决的问题。以小麦细胞为研究材料,应用常规电子显微镜技术,观察了小麦细胞核仁纤维中心(Fibrillar centers,FC)内染色质的超微结构;并通过DNA抗体阐明了核仁中DNA位于纤维中心、致密纤维组分(Dense fibrillar component,DFC)以及两者的过渡区域;应用RNA聚合酶I相关转录因子UBF(Upstream binding factor)抗体所做的分析显示,小麦细胞核仁中UBF位于FC与DFC的过渡区域以及DFC中,在FC中没有UBF的存在;进一步借助于RNA/DNA杂合体抗体选择性地直接标记核仁中rRNA基因的转录位点,结果表明了小麦细胞核仁rRNA基因的转录位点是在FC与DFC的过渡区域及DFC中。

 
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