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j gene
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  “j gene”译为未确定词的双语例句
     The Screening of the Apolipoprotein J Gene in Patients with Pregnancy Induced Hypertension Syndrome
     妊娠高血压综合征患者载脂蛋白J基因的筛查
短句来源
     Study on association of apolipoprotein J gene polymorphism with type 2 diabetes mellitus
     载脂蛋白J基因多态性与2型糖尿病相关性分析
短句来源
     The association between apolipoprotein J gene polymorphism and type 2 diabetes
     载脂蛋白J基因多态性与2型糖尿病相关性分析
短句来源
     The compound of RAG-1 and RAG-2 can identify specifically recombination signal sequence which lie two sides of the V、 DH、J gene segment in Ig germline gene, then break DNA and induce the recombination of V(D)J gene segment.
     RAGs的编码产物RAG-1和RAG-2的复合物能特异性识别Ig胚系基因中V、DH、J基因片段两侧的重组信号序列,切断DNA双链,引发V(D)J基因片段重排。
短句来源
     The study of apolipoprotein J gene polymorphism by PCR-DGGE showed that 31 patients with T2DM had higher frequencies of mutation heterozygotes in exon 5 (100% vs 76.7%) and in exon 2 (61.2% vs 40.0%), as compared with normal control (n=30).
     用PCR变性梯度凝胶电泳法研究载脂蛋白J基因外显子2和5的多态性,发现31例2型糖尿病(T2DM)病人的外显子5的突变杂合子频度高于正常对照(n=30)(100%vs77%,P<0.05),外显子2的突变杂合子频度也高于正常对照(61%vs40%)。
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  相似匹配句对
     -casein gene.
     -酪蛋白基因5'侧序列。
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     The gene smu.
     变形链球菌smu.
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  j gene
In this paper, we analyze a particular stretch of 69 kb centromeric to the HLA-J gene, in which we identify 21 different mRNAs mainly expressed in testis, and characterize five different transcription units, HZFw, HZFc, HCGV, HTEX6, and HTEX4.
      
Noticeably, the subtilisin Pr1J gene from different strains of M.ansiopliae contained different number of introns, the strong evidence in support of introns-late theory.
      
A map-based cloning scheme is being used to isolate the jointless (j) gene of tomato.
      
This mutant, designated insJ, was viable only in the presence of a wild-type J gene carried on a plasmid that could provide J protein.
      
A ?X174 mutant was constructed by inserting an 11 nucleotide sequence into the J gene.
      
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The S-type CMS of maize was reported to be caused by a DNA region designated R in mitochondrial genomes. Through PCR using artificial primers, 1kb DNA fragments were obtained from mitochondrial DNA of Tangxu and WB cytoplasm.The DNA sequences are almost the same as the sequence in R region of CMS-S. Using the DNA fragment from Tangxu cytoplasm as probe to scan the Mo17 CMS-J gene library, several positive clones appeared. The clone B39 contains a 6.7kb BamHI fragment with R region. Nucleotide sequence...

The S-type CMS of maize was reported to be caused by a DNA region designated R in mitochondrial genomes. Through PCR using artificial primers, 1kb DNA fragments were obtained from mitochondrial DNA of Tangxu and WB cytoplasm.The DNA sequences are almost the same as the sequence in R region of CMS-S. Using the DNA fragment from Tangxu cytoplasm as probe to scan the Mo17 CMS-J gene library, several positive clones appeared. The clone B39 contains a 6.7kb BamHI fragment with R region. Nucleotide sequence analysis found two orfs including the chimeric orf77. Seventeen lines of maize under Mo17 and 77 nuclear background including N, T, C, S four types of cytoplasm were used for RFLP. Three types of R were discovered in S-type CMS, but not in C group. The R in N group was different from that in S group. T group had only part of R region. The Southern blot of Tangx and Shuang cytoplasm in R regions showed different ratio of R in different nuclear backgrounds. The heterogeneity of R region suggests that the organization and rearrangement of R controlled by the nuclear background. R region seems to be involved in the mechanism of S group CMS. Varieties of structures of R, the ratio of three R types, different DNA sequences in R region of different CMS mightbe concerned with instability of S-type CMS.

玉米S组细胞质雄性不育(CMS)可能与线粒体基因组中的R区域有关。对不同核背景下唐徐、双2种S胞质的线粒体DNA以R区特异探针的Southern分析发现均有6.7kb、4.5kb、1.8kb的3条谱带,分别对应于2种位于线粒体基因组中间的类型和1个线性末端,并且核背景对这3种不同形式的R区域的量有影响。对Mo17和77核背景下N、T、C_4种胞质17种材料的玉米线粒体基因组中R区的Southern分析发现在C组CMS中不存在R序列,T组中可能只存在R的部分片段,N、S组中都存在R区但有长度上的差别。还观察到了在Mo17和77核背景下不同来源的S胞质材料包括从国外引进的如CMS朣、Vg和在国内玉米品种中发现的CMS材料如唐徐等有着相同的R区结构。用R区域特异引物从S胞质材料唐徐、WB中扩增出了R区的1个1kb的片段并进行DNA序列测定,以此DNA片段为探针从Mo17 CMS朖线粒体基因文库中筛选出并亚克隆了6.7kb的完整的BamHI酶切片段。DNA序列分析表明在R区内有2个开放阅读框(orf),其中orf77中有3段序列与玉米线粒体atp9基因的编码区和3′侧翼有同源性。在6.7kb片段的R区上游的DNA序列...

玉米S组细胞质雄性不育(CMS)可能与线粒体基因组中的R区域有关。对不同核背景下唐徐、双2种S胞质的线粒体DNA以R区特异探针的Southern分析发现均有6.7kb、4.5kb、1.8kb的3条谱带,分别对应于2种位于线粒体基因组中间的类型和1个线性末端,并且核背景对这3种不同形式的R区域的量有影响。对Mo17和77核背景下N、T、C_4种胞质17种材料的玉米线粒体基因组中R区的Southern分析发现在C组CMS中不存在R序列,T组中可能只存在R的部分片段,N、S组中都存在R区但有长度上的差别。还观察到了在Mo17和77核背景下不同来源的S胞质材料包括从国外引进的如CMS朣、Vg和在国内玉米品种中发现的CMS材料如唐徐等有着相同的R区结构。用R区域特异引物从S胞质材料唐徐、WB中扩增出了R区的1个1kb的片段并进行DNA序列测定,以此DNA片段为探针从Mo17 CMS朖线粒体基因文库中筛选出并亚克隆了6.7kb的完整的BamHI酶切片段。DNA序列分析表明在R区内有2个开放阅读框(orf),其中orf77中有3段序列与玉米线粒体atp9基因的编码区和3′侧翼有同源性。在6.7kb片段的R区上游的DNA序列包括有cox_II基因,这种结构不同于已报道的R区的结构。R区的特点可能与S组的CMS形成机理和育性不稳定性有关。

This paper summarized the results of the research on the gene expression profile in adult worm of Schistosoma japonicum(S.j) Mainland strain from 1998 to 2000 The main results are as follows: The EST approach has provided partial sequences of 551 clones Among them, 519 had been deposited in dbEST, which were grouped as 388 different expressed genes of S.j Homologous searching for significant similarities to sequences deposited in schistosoma database of EMBL, and GenBank with the...

This paper summarized the results of the research on the gene expression profile in adult worm of Schistosoma japonicum(S.j) Mainland strain from 1998 to 2000 The main results are as follows: The EST approach has provided partial sequences of 551 clones Among them, 519 had been deposited in dbEST, which were grouped as 388 different expressed genes of S.j Homologous searching for significant similarities to sequences deposited in schistosoma database of EMBL, and GenBank with the basic local alignment search tool program (Blastn & Blastx) showed that 24 ESTs (6 19%) represented genes previously characterized in S.j, 18 ESTs(4 64%) similar to the sequences of Schistosoma mansoni, 90 ESTs(23 19%) similar to the known gene of other species except schistosome, these homologous genes included arginase gene, amidase gene, fibrillin gene, actin gene, cytochrome c oxidase gene, disulfide isomerase gene, elongin gene, heat shock protein 70 gene, Y box binding protein gene and DAD1(Defender Against Apoptotic Death 1) gene etc 256 ESTs(65 98%) of ESTs had no significant matches with sequences of schistosoma or other species, which were considered to be the unidentified genes or newly characterized genes of this organism For a further characterization of those clones, full length sequencing of interesting clones were carried out Some novel S.j genes such as arginase, S.j Y box binding protein and DAD1 genes had been cloned and sequenced, their accession numbers were AF402615, AF367371 and AF333765 respectively The study provides the information about the gene expression profile in S.j adult worm, and adds the sequence data for schistosoma genome database as well as screening of Schistosomiasis vaccine and drug development

报告作者 1998年至 2 0 0 0年有关日本血吸虫 (大陆株 )成虫基因表达谱研究的结果 ,主要包括 :已测定 5 5 1条EST ,其中 5 19条EST已送入国际基因数据库 (GenBank/dbEST) ,并获得了GenBank的登录号。经同源整合后 ,5 19条EST序列归类为 388个基因序列 ,其中 ,2 4条 (6 19%)为日本血吸虫已知基因序列 ;18条 (4 6 4 %)EST与曼氏血吸虫已知基因序列同源 ,90条 (2 3 19%)EST与其它生物的已知基因序列同源 ,同源基因包括精氨酸酶、酰胺酸酶、微纤维蛋白、肌动蛋白、细胞色素C氧化酶、二硫异构酶、加长因子、热激蛋白 70、Y盒结合蛋白和抗凋亡因子等。其余的 2 5 6条 (6 5 98%)EST在Gen Bank中未发现有已知基因的同源序列。将非日本血吸虫同源序列EST或未检索到同源序列的EST初步定义为日本血吸虫亲基因 /未知基因序列EST ,共 36 4条。已克隆日本血吸虫精氨酸酶基因、Y 盒结合蛋白基因和抗凋亡 1因子 3个新基因的全长cDNA ,其GenBank的登录号分别为AF4 0 2 6 15、AF3...

报告作者 1998年至 2 0 0 0年有关日本血吸虫 (大陆株 )成虫基因表达谱研究的结果 ,主要包括 :已测定 5 5 1条EST ,其中 5 19条EST已送入国际基因数据库 (GenBank/dbEST) ,并获得了GenBank的登录号。经同源整合后 ,5 19条EST序列归类为 388个基因序列 ,其中 ,2 4条 (6 19%)为日本血吸虫已知基因序列 ;18条 (4 6 4 %)EST与曼氏血吸虫已知基因序列同源 ,90条 (2 3 19%)EST与其它生物的已知基因序列同源 ,同源基因包括精氨酸酶、酰胺酸酶、微纤维蛋白、肌动蛋白、细胞色素C氧化酶、二硫异构酶、加长因子、热激蛋白 70、Y盒结合蛋白和抗凋亡因子等。其余的 2 5 6条 (6 5 98%)EST在Gen Bank中未发现有已知基因的同源序列。将非日本血吸虫同源序列EST或未检索到同源序列的EST初步定义为日本血吸虫亲基因 /未知基因序列EST ,共 36 4条。已克隆日本血吸虫精氨酸酶基因、Y 盒结合蛋白基因和抗凋亡 1因子 3个新基因的全长cDNA ,其GenBank的登录号分别为AF4 0 2 6 15、AF36 7371和AF33376 5。本研究结果丰富了血吸虫基因组数据 ,并为进一步筛选日本血吸虫疫苗候选基因或药物靶位提供了科学依据。

A PCR for rapid and specific detection of Streptococcus suis type 2 was developed. The primers based on the cps2J-gene of S.suis type 2 could extend a 675 bp PCR product. Of 9 strains of S.suis type 2 isolated from diseased pigs, all were positive by the PCR. Using the same pairs, no PCR product was detected from S.equi subsp zooepidemicus, Staphylococcus hyicus, E.rhusiopathiae, P.pneumotropica and M.hyopneumoniae. Of 88 porcine tonsillar specimens, 36 was positive by the PCR or glass agglutination...

A PCR for rapid and specific detection of Streptococcus suis type 2 was developed. The primers based on the cps2J-gene of S.suis type 2 could extend a 675 bp PCR product. Of 9 strains of S.suis type 2 isolated from diseased pigs, all were positive by the PCR. Using the same pairs, no PCR product was detected from S.equi subsp zooepidemicus, Staphylococcus hyicus, E.rhusiopathiae, P.pneumotropica and M.hyopneumoniae. Of 88 porcine tonsillar specimens, 36 was positive by the PCR or glass agglutination test. The corresponding rate was 100%. The results demonstrated that the PCR is a highly specific and rapid diagnostic tool for detection of S.suis type 2 and can be used to fast diagnosis and epidemic survey.

根据猪链球菌 2型的荚膜多糖抗原基因 cps2 J,合成 1对可扩增长度为 6 75 bp目的片段的引物 ,建立了检测猪链球菌 2型的 PCR法。应用 PCR对 9株经玻片凝集试验检测为猪链球菌 2型的菌株进行了检测 ,均呈阳性 ;而对马链球菌兽疫亚种 (C群 )、猪葡萄球菌、猪丹毒杆菌、猪肺疫巴氏杆菌、猪肺炎霉形体等检测结果均呈阴性 ,表明了本方法的特异性。用此法对 88份正常猪的扁桃体样品的细菌分离物进行了检测 ,36份呈阳性 ,同时用玻片凝集试验进行对照检测 ,也全部呈阳性。而此法不需进行细菌的纯分离培养 ,即可用于猪链球菌 2型的快速诊断以及流行病学调查。

 
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